scholarly journals In vitro culture of Spondias mombin L. nodal segments

2002 ◽  
Vol 24 (3) ◽  
pp. 776-777 ◽  
Author(s):  
Cristina Paiva da Silveira Carvalho ◽  
Diva Correia ◽  
Abdellatif Kemaleddine Benbadis ◽  
José Magno Queiroz Luz ◽  
Adroaldo Guimarães Rossetti
Keyword(s):  
Root Formation ◽  
Shoot Formation ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Shoot Cultures ◽  
Nodal Explant ◽  
Spondias Mombin ◽  
First Response ◽  
Wide Range

Spondias mombin L. shoot cultures were initiated from nodal explants taken from plants propagated by seeds. Explants coming from 4-6 months old plants, previously disinfected, were cultivated on WPM medium supplemented with a wide range of concentrations of BAP (0.0, 0.22, 0.44, 2.22 and 4.44 muM) and NAA (0.0, 0.27 and 2.70 muM). After four weeks, the responses obtained were axillary shoot and root formation. The first response were preferentially induced with the medium containing only BAP, regardless of the BAP concentration. The addition of NAA on medium reduced significantly axillary shoot formation and induced rhizogenesis. Roots were formed on nodal explant basis, preferentially on medium supplemented with 4.44 muM NAA. The medium supplemented with BAP reduced significantly root formation.

scholarly journals ORGANOGENESIS IN CULTURED ADVENTITIOUS ROOT SEGMENTS AND IN PROTOPLAST-DERIVED CALLUS OF SWEET POTATO

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1121a-1121 ◽  
Author(s):  
Peggy Ozias-Akins ◽  
Srini Perera
Keyword(s):  
Sweet Potato ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Ipomoea Batatas ◽  
Root Formation ◽  
Shoot Formation ◽  
Root Segment ◽  
Shoot Cultures ◽  
Mesophyll Tissue

One cm segments from adventitious roots of sweet potato (Ipomoea batatas (L.) Lam.) will regenerate shoots when cultured on Murashige and Skoog salts and vitamins plus either sucrose (1-3%) or fructose (1-6%). The best source for adventitious roots is sweet potato shoot cultures maintained in Magenta vessels. A low concentration of cytokinin (0.02 mg/liter) promotes shoot formation. Higher levels of cytokinin (0.1-0.5 mg/liter) encourage callus growth. The maximum average number of shoots formed per root segment attained thus far is 0.5. Attempts are being made to increase the frequency of shoot formation. Regeneration of shoots from roots also may be a useful method for obtaining plants from protoplasts of sweet potato. Protoplasts can be isolated from mesophyll tissue and petioles of in vitro grown plants. Plating efficiency of up to 12% routinely can be obtained. Shoot formation directly from callus is sporadic; root formation is more frequent.

scholarly journals Growth and In Vitro Propagation of Peach Plants Transformed with the Shooty Mutant Strain of Agrobacterium tumefaciens

HortScience ◽  
1998 ◽  
Vol 33 (5) ◽  
pp. 897-899 ◽  
Author(s):  
F.A. Hammerschlag ◽  
A.C. Smigocki
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Mutant Strain ◽  
In Vitro Propagation ◽  
Prunus Persica ◽  
Shoot Formation ◽  
Fresh Mass ◽  
Axillary Shoot ◽  
Shoot Cultures ◽  
Cytokinin Biosynthesis

Peach [Prunus persica (L.) Batsch] plants #94-1, #99-1, and #40-1, carrying a cytokinin biosynthesis (ipt) gene following transformation with the shooty mutant strain of Agrobacterium tumefaciens, were evaluated for altered growth habit and axillary shoot formation, both in vitro and in the greenhouse. After 9 weeks of in vitro propagation on four different levels of 6-benzyladenine (BA), only transformant #99-1 exhibited significantly greater axillary shoot formation (on 10 μm BA), and significantly greater fresh mass (on 3,10, and 30 μm BA) than the control #RG-3. Tolerance to a supra-optimal (30 μm) concentration of BA was indicated by fresh mass increases for #99-1 shoot cultures. Delayed senescence on 0 μm BA was exhibited by 87% of the transformants, but by only 12% of the control plants. Greenhouse-grown #99-1 and #40-1 were significantly shorter than #RG-3 plants at 6 weeks and at 1 year, but only #40-1 exhibited significantly greater branching than the controls. Chemical names used: 6-benzyladenine (BA); isopentenyl transferase (ipt).

scholarly journals In vitro Axillary Shoot Regeneration and Direct Protocorm-like Body Induction from Axenic Shoot Tips of Doritis pulcherrima Lindl.

2014 ◽  
Vol 23 (2) ◽  
pp. 251-261 ◽  
Author(s):  
Tustu Mondal ◽  
Sumana Aditya ◽  
Nirmalya Banerjee
Keyword(s):  
Root Formation ◽  
Shoot Formation ◽  
Shoot Tips ◽  
Tree Bark ◽  
Axillary Shoot ◽  
Inhibitory Effects ◽  
Terrestrial Orchid ◽  
Root Regeneration ◽  
Shoot Tip Explants

An efficient protocol for in vitro propagation of an important ornamental terrestrial orchid, Doritis pulcherima Lindl. through axillary shoot and direct protocorm-like body (PLB) formation from shoot tip explants derived from six- month-old axenic seedlings has been described. Shoot tips were cultured on modified nutrient medium of Knudson’s C supplemented with 0.1% peptone and combination of various concentrations of NAA and BAP. The effect of NAA and BAP on axillary shoot formation, protocorm-like body induction and root regeneration from the explants was significant. The highest frequency of axillary shoot formation was recorded in the medium containing 2 mg/l BAP and the PLB production was higher in the medium containing 2 mg/l NAA. A higher concentration of BAP showed inhibitory effects on the axillary shoot formation and PLB induction. Efficient root regeneration was observed in low concentration of NAA. However, the profuse root formation was common in the PGR free medium. Rooted plantlets were hardened successfully through the stepwise acclimation protocol and platelets were finally established in the potting mixture containing small pieces of dead tree bark of mango, charcoal pieces and broken bricks in 1 : 2 : 1 ratio. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17526 Plant Tissue Cult. & Biotech. 23(2): 251-261, 2013  (December)

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals Rhizogenesis in in vitro shoot cultures of passion fruit (Passiflora edulis f. flavicarpa Deg.) is affected by ethylene precursor and by inhibitors

2001 ◽  
Vol 7 (1) ◽  
Author(s):  
W. M. Marota ◽  
W. C. Otoni ◽  
M. Carnelossi ◽  
E. Silva ◽  
A. A. Azevedo ◽  
...  
Keyword(s):  
Ethylene Production ◽  
Time Course ◽  
Root Formation ◽  
Callus Formation ◽  
Passion Fruit ◽  
Shoot Cultures ◽  
Ethylene Inhibitors ◽  
Beneficial Effects ◽  
Ethylene Precursor

The effects of the ethylene precursor ACC and two inhibitors, AgNO3 and AVG, on root formation were tested in in vitro shoots of passion fruit (Passiflora Midis f.flavicalpa Deg.). The organogenic response was assessed on the basis of percentage of shoot-forming. roots, root number and length. The time course of ethylene production was also monitored. ACC inhibited root formation by delaying root emergence and increasine, callus formation at the basis of the shoots. In addition, ACC caused a marked increase in ethylene production, coupled to leaf chlorosis and senescence with lower rooting frequencies, number and length of roots. IAA supplementation increased ethylene production. Both ethylene inhibitors, AgNO3 and AVG, at appropriate concentrations reduced callus formation at the basis of shoots. AVG increased the number of roots per shoot, but drastically reduced length of differentiated roots. Regarding to leaf pigments, ACC promoted a marked reduction on carotenoids and total chlorophyll, whereas AVG and AgNO3 delayed explant senescence and pigments degradation, not differing from IAA supplemented and non-supplemented control treatments. The results confirm previous reports on the beneficial effects of ethylene inhibitors on in vitro rooting and suggest its reliability to be used as an alternative approach to evaluate sensitivity of Passiflora species to ethylene.  

scholarly journals Effect of 6-BA on nodal explant bud sproutings of Coffea arabica cv. Mundo Novo

Bragantia ◽  
2005 ◽  
Vol 64 (2) ◽  
pp. 185-190
Author(s):  
Luis Carlos da Silva Ramos ◽  
Julieta Andrea Silva de Almeida
Keyword(s):  
Coffea Arabica ◽  
Multiple Shoots ◽  
Nodal Explants ◽  
Nodal Explant ◽  
In Vitro Plantlets ◽  
Lateral Buds ◽  
Bud Sprouting ◽  
Wide Range ◽  
Coffee Plants

Coffee plants can be micropropagated by nodal bud sprouting using the 6-benzylaminopurine (6-BA) hormone. However, literature reports the use of a wide range of 6-BA, from 0.5 to 88.8 µM L-1. So, this study was performed to narrow that range. Nodal explants of Coffea arabica cv Mundo Novo obtained from in vitro plantlets were inoculated on gelled-MS medium supplemented with different concentrations of 6-BA. Two assays were carried out: in the first one, 6-BA was used at concentrations of 0, 5, 25, 50, and 100 µM L-1, being evaluated at 43 and 123 days. In the second experiment, dosis of 10, 20 and 30 µM L-1, have evaluated at 65 and 100 days. Treatments with 6-BA induced multiple sprouting from the nodal explants, which were best characterized around 100 days after inoculation. The nodal explants grew taller and showed multiple shoots, whereas the effect of 6-BA at 5 to 25 µM L-1 was similar to that with higher concentrations (50 and 100 µM L-1). Nodal explants yielded from 2.9 to 6.0 buds per node, achieving height of 1.3 to 1.5 cm at 5 to 25 µM L-1 of 6-BA, whereas they yielded from 4.3 to 4.9 buds per node but the sprouting grew about 0.8 cm at 50 and 100 µM L-1 of 6-BA. This study indicated that multiple sprouting of lateral buds can be induced by lower concentrations of 6-BA, for example, from 10 to 30 µM L-1, diminishing possible risks of somaclonal variation due to high levels of hormone concentration.

scholarly journals Vegetative Propagation of Phytophthora cinnamomi-Tolerant Holm Oak Genotypes by Axillary Budding and Somatic Embryogenesis

Forests ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 841
Author(s):  
Maria Teresa Martínez ◽  
Francisco Javier Vieitez ◽  
Alejandro Solla ◽  
Raúl Tapias ◽  
Noelia Ramírez-Martín ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Phytophthora Cinnamomi ◽  
Shoot Proliferation ◽  
Holm Oak ◽  
Induction Medium ◽  
Axillary Shoot ◽  
Oak Decline ◽  
Shoot Cultures

Holm oak (Quercus ilex) is one of the most widely distributed tree species in the Mediterranean basin. High mortality rates have been observed in holm oak populations in the southwest of the Iberian Peninsula as a result of oak decline syndrome. Selection and propagation of genotypes tolerant to this syndrome could aid the restoration of affected areas. In this article, we report micropropagation and conservation procedures based on axillary budding and somatic embryogenesis (SE) of holm oak plants, selected for their tolerance to Phytophthora cinnamomi—the main biotic factor responsible for oak decline. Forced shoots were obtained from potted plants of eight different genotypes, and used as stock material to establish in vitro shoot proliferation cultures. Reliable shoot proliferation was obtained in seven out the eight genotypes established in vitro, whereas multiplication rates were genotype-dependent. The highest rooting rates were obtained by culturing shoots for 24 h or 48 h on rooting induction medium containing 25 mg L−1 indole-3-butyric acid, followed by transfer to medium supplemented with 20 µM silver thiosulphate. Axillary shoot cultures can be successful conserved by cold storage for 12 months at 4 °C under dim lighting. Shoot tips, excised from axillary shoot cultures established from tolerant plants, were used as initial explants to induce SE. Somatic embryos and/or nodular embryogenic structures were obtained on induction medium with or without indole-acetic acid 4 mg L−1, in two out the three genotypes evaluated, and induction rates ranged between 2 and 4%. Plantlet recovery was 45% after two months cold stratification of somatic embryos and eight weeks of culture on germination medium. Vegetative propagation of P. cinnamomi-tolerant Q. ilex trees is a valuable milestone towards the restoration of disease-affected areas.

scholarly journals Plant Regeneration Through Nodal Explant Derived Callus in Wood Apple (Aegle marmelos L.)

1970 ◽  
Vol 44 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Ranjoy Das ◽  
M Faruk Hasan ◽  
Harunar Rashid ◽  
Motiur Rahman
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Nodal Explant ◽  
Aegle Marmelos ◽  
Half Strength ◽  
Subsequent Regeneration

This study reports on an improved protocol for callus induction and subsequent regeneration from nodal segment of wood apple (Aegle marmelos L.) Creamish friable competent callus was achieved from nodal segments on MS medium augmented with 4.0 mg1-1 2,4-D within two weeks of inoculation. The callus produced large number of shoots when cultured on MS medium fortified with 2.0 mgl-1 BAP+0.1 mgl-1 NAA within ten days of culture. In vitro raised shoots were rooted on half strength MS medium enriched with 1.0 mgl-1 IBA within fifteen days of culture. The rooted plantlets were successfully established with 80% survival. Key words: Plant regeneration; Callus induction; Nodal explant; Aegle marmelos. DOI: 10.3329/bjsir.v44i4.4590 Bangladesh J. Sci. Ind. Res. 44(4), 415-420, 2009

scholarly journals Micropropagation of Genista aetnensis [(Raf. ex Biv.)DC]

2015 ◽  
Vol 43 (2) ◽  
pp. 542-546 ◽  
Author(s):  
Giovanni IAPICHINO ◽  
Marcello AIRÒ ◽  
Emilio LO PRESTI ◽  
Leo SABATINO
Keyword(s):  
Acetic Acid ◽  
Shoot Proliferation ◽  
Shoot Development ◽  
In Vitro Rooting ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
High Plasticity ◽  
Single Node ◽  
Indole 3 Acetic Acid

Genista aetnensis [(Raf. ex Biv.)DC] is a large deciduous shrub or small tree native to the Italian islands of Sardinia and Sicily. Being winter hardy and characterized by high plasticity in altitude and ecology, the species is grown in gardens and landscaping, both for flower and for its attractive shape. Genista species are generally propagate by seed or semi-hardwood cuttings. In this report an efficient in vitro technique for propagation of G. aetnensis was investigated. Multiple shoots were induced on nodal segments of a mature plant of Genista aetnensis. The Murashige and Skoog medium, augmented with different concentrations of N-6-benzyladenine either singly or in combination with indole-3-acetic acid, as potential medium for shoot multiplication by nodal segments was tested. In the following experiment equal molar concentrations of four cytokinins (2-isopenthenyladenine, kinetin, zeatin and N-6-benzyladenine) were tested for ability to induce axillary shoot development from single node stem segments. The highest rate of axillary shoot proliferation was induced on the medium supplemented with 0.44 µM BA. Growth regulator requirements for shoot proliferation in G. aetnensis were satisfied by BA alone. Explants were divided, subcultured and continued to proliferate shoots. A proliferation rate of 3.5 shoots per single node explants every four weeks occurred. Seven indole-3-acetic acid concentrations (0, 0.23, 0.45, 0.91, 1.82, 3.64 or 7.29 µM) were tested to determine the optimum conditions for in vitro rooting of microshoots. The highest rooting percentage was obtained with indole-3-acetic acid at 3.64 mM (57%). Eighty percent of the in vitro rooted plantlets were successfully established in soil. This micropropagation system of G. aetnensis based on axillary shoot development from nodal segments followed by in vitro rooting should be preferred for rapid and efficient mass propagation of selected clones and could represent an alternative method to sexual and conventional asexual propagation.

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