scholarly journals Rhizogenesis in in vitro shoot cultures of passion fruit (Passiflora edulis f. flavicarpa Deg.) is affected by ethylene precursor and by inhibitors

2001 ◽  
Vol 7 (1) ◽  
Author(s):  
W. M. Marota ◽  
W. C. Otoni ◽  
M. Carnelossi ◽  
E. Silva ◽  
A. A. Azevedo ◽  
...  
Keyword(s):  
Ethylene Production ◽  
Time Course ◽  
Root Formation ◽  
Callus Formation ◽  
Passion Fruit ◽  
Shoot Cultures ◽  
Ethylene Inhibitors ◽  
Beneficial Effects ◽  
Ethylene Precursor

The effects of the ethylene precursor ACC and two inhibitors, AgNO3 and AVG, on root formation were tested in in vitro shoots of passion fruit (Passiflora Midis f.flavicalpa Deg.). The organogenic response was assessed on the basis of percentage of shoot-forming. roots, root number and length. The time course of ethylene production was also monitored. ACC inhibited root formation by delaying root emergence and increasine, callus formation at the basis of the shoots. In addition, ACC caused a marked increase in ethylene production, coupled to leaf chlorosis and senescence with lower rooting frequencies, number and length of roots. IAA supplementation increased ethylene production. Both ethylene inhibitors, AgNO3 and AVG, at appropriate concentrations reduced callus formation at the basis of shoots. AVG increased the number of roots per shoot, but drastically reduced length of differentiated roots. Regarding to leaf pigments, ACC promoted a marked reduction on carotenoids and total chlorophyll, whereas AVG and AgNO3 delayed explant senescence and pigments degradation, not differing from IAA supplemented and non-supplemented control treatments. The results confirm previous reports on the beneficial effects of ethylene inhibitors on in vitro rooting and suggest its reliability to be used as an alternative approach to evaluate sensitivity of Passiflora species to ethylene.  

Ethylene inhibitors enhance in vitro root formation from apple shoot cultures

1998 ◽  
Vol 17 (3) ◽  
pp. 211-214 ◽  
Author(s):  
J.-H. Ma ◽  
J.-L. Yao ◽  
D. Cohen ◽  
B. Morris
Keyword(s):  
Root Formation ◽  
Shoot Cultures ◽  
Ethylene Inhibitors ◽  
Apple Shoot

THE ROLE OF CYTOKININS AND ETHYLENE INHIBITORS ON LENTICEL HYPERTROPHY GENERATION AND ETHYLENE PRODUCTION IN IN VITRO CULTURES OF POPULUS EUPHRATICA OLIVIER

1995 ◽  
Vol 43 (4) ◽  
pp. 339-345 ◽  
Author(s):  
M.D. Lledó ◽  
M.B. Crespo ◽  
J.B. Amo-Marco
Keyword(s):  
Ethylene Production ◽  
Populus Euphratica ◽  
In Vitro Cultures ◽  
Nodal Segments ◽  
Ethylene Inhibitors ◽  
Se Spain ◽  
Ethylene Formation ◽  
Free Media

Populus euphratica Olivier is native to the Irano—Turanian areas (Middle East). Elche (Alicante province, SE Spain) is known to be its only European location. Nodal segments from root shoots were established in vitro in a Murashige and Skoog medium supplemented with several cytokinins. Ethylene inhibitors AgNO3 and CoCl2 were used in combination with kinetin. Hormone-free media supplemented with sucrose (20–60 mg 1−1) was also tested. Ethylene was measured by gas chromatography, and both the percentage of sprouting shoots and lenticel hypertrophy in cultures were recorded. Ethylene production was higher in cultures supplemented with cytokinins (especially with meta-topolin), with high sprouting percentages, and lenticel hypertrophy. In cultures supplemented with 6-benzylaminopurine or 6-(γ,γ,-dimethylallylamino)-purine, ethylene production was lower and explants looked unhealthy. Ethylene formation was inhibited in cultures supplemented with AgNO3 (1 mg 1−1), which also decreased percentage of sprouting buds and lenticel hypertrophy.

Effect of Ethylene Inhibitors on in vitro Shoot Multiplication and their Impact on Ethylene Production in Cucumber (Cucumis sativus L.)

2006 ◽  
Vol 1 (3) ◽  
pp. 110-117 ◽  
Author(s):  
Vasudevan Ayyappan . ◽  
Selvaraj Natesan . ◽  
Ganapathi Andy . ◽  
Chang won Choi . ◽  
Manickavasagam Marka . ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Ethylene Production ◽  
Shoot Multiplication ◽  
Ethylene Inhibitors ◽  
Cucumis Sativus L

scholarly journals Effect of plant growth regulators on the root formation in in vitro culture of Abelmoschus Sagittifolius Kurz

2020 ◽  
Vol 4 (2) ◽  
pp. First
Author(s):  
Trân Huỳnh Ngọc Dương ◽  
Diễm Thị Lê ◽  
Mai Thị Bạch Võ
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Root Formation ◽  
Callus Formation ◽  
Plant Root ◽  
Germination Rate ◽  
Distribution Area ◽  
Increasing Demand

Abelmoschus sagittifolius Kurz is a medicinal plant with typical pharmacological of ginseng. However, the number of trees in the nature wild is declining rapidly due to the increasing demand for logging along with the narrowing of the distribution area and the low incidence of seed germination, affecting the use for researching and developing gene sources for drug production in many areas. In this plant, root is the most important organ of the plant, so the study of root formation in in vitro has been of great significance in assessing the effect of plant growth regulators on induction roots, as well as creating a source of starting material for studies on the biosynthesis of saponin in in vitro compounds as an alternative to outside planting. The results showed that after 2 weeks of culture, the germination rate was highest (88%) when the seeds were disinfected with HgCl2 0.1%, 3 minutes and then soaked in GA3 20,0 mg/L, 120 minutes, finally seed culture on MS + 20 g/L saccharose + GA3 5.0 mg/L + 7 g/L agar. The callus formation from hypocotyl in the environment on MS medium + 20 g/L sucrose + NAA 0.5 mg/L + BA 1.5 mg/L + 7 g/L agar was appropriate for root reduction and the best root formation was applied in the medium of MS + 20 g/L sucrose + IAA 0.3 mg/L + 7 g/L agar. In conclusion, the method of tissue culture is suitable for the formation of adventitious roots from callus formation from hypocotyl of Abelmoschus sagittifolius Kurz.

scholarly journals Identifying Beneficial Qualities ofTrichoderma parareeseifor Plants

2014 ◽  
Vol 80 (6) ◽  
pp. 1864-1873 ◽  
Author(s):  
M. Belén Rubio ◽  
Narciso M. Quijada ◽  
Esclaudys Pérez ◽  
Sara Domínguez ◽  
Enrique Monte ◽  
...  
Keyword(s):  
Time Course ◽  
Plant Cell Wall ◽  
Growth Promotion ◽  
Expression Patterns ◽  
Hyphal Growth ◽  
Defense Responses ◽  
Tomato Plants ◽  
Lateral Root Development ◽  
Beneficial Effects

ABSTRACTTrichoderma parareeseiandTrichoderma reesei(teleomorphHypocrea jecorina) produce cellulases and xylanases of industrial interest. Here, the anamorphic strain T6 (formerlyT. reesei) has been identified asT. parareesei, showing biocontrol potential against fungal and oomycete phytopathogens and enhanced hyphal growth in the presence of tomato exudates or plant cell wall polymers inin vitroassays. ATrichodermamicroarray was used to examine the transcriptomic changes in T6 at 20 h of interaction with tomato plants. Out of a total 34,138Trichodermaprobe sets deposited on the microarray, 250 showed a significant change of at least 2-fold in expression in the presence of tomato plants, with most of them being downregulated.T. parareeseiT6 exerted beneficial effects on tomato plants in terms of seedling lateral root development, and in adult plants it improved defense againstBotrytis cinereaand growth promotion under salt stress. Time course expression patterns (0 to 6 days) observed for defense-related genes suggest that T6 was able to prime defense responses in the tomato plants against biotic and abiotic stresses. Such responses undulated, with a maximum upregulation of the jasmonic acid (JA)/ethylene (ET)-relatedLOX1andEIN2genes and the salt toleranceSOS1gene at 24 h and that of the salicylic acid (SA)-relatedPR-1gene at 48 h after T6 inoculation. Our study demonstrates that theT. parareeseiT6-tomato interaction is beneficial to both partners.

scholarly journals In vitro CULTURE OF BIG-SAGE (Lantana camara L.) PLANT

2020 ◽  
Vol 19 (2) ◽  
pp. 67-73
Author(s):  
Majid Abdulhameed Ibrahim ◽  
Manal Zebari Sabty ◽  
Shaimaa Hussein Mussa
Keyword(s):  
Root Length ◽  
Root Formation ◽  
Callus Formation ◽  
Shoot Multiplication ◽  
Lantana Camara ◽  
The Other ◽  
Ms Medium ◽  
Formation Number ◽  
Number Of Shoots

The study was conducted to mass micropropagation of big sage (Lantana camara L.) plant by shoot multiplication technique. The treatments 2.22 and 2.66 µmol·L–1 BA gave the highest significant increase in the percentage of response to shoot multiplication and number of shoots per explant compared to the other treatments as reached 96.70% and 100.00% and 4.33 and 6.00 shoots, respectively. The results showed that these two treatments did not differ significantly between them. While the 1.33 µmol·L–1 BA gave the lowest values in the percentage of response to shoot multiplication and number of shoots per explant were 80.00% and 2.00 shoots per explant, respectively. The MS medium supplemented with 4.30 or 5.37 µmol·L–1 NAA gave a high response to root formation, number of roots per shoot and root length. While the MS medium supplemented with 6.44 or 7.52 µmol·L–1 NAA gave low values in these characteristics. The MS medium with 2.22 or 2.66 µmol·L–1 concentration of BA or 7.52 µmol·L–1 concentration of NAA recorded the highest significant increase in the percentage of response to callus formation. While the MS medium supplemented with 1.33 µmol·L–1 BA or 4.30 µmol·L–1 NAA gave less response to the callus formation.

scholarly journals Pyroligneous Acid Improves In Vitro Rooting of Japanese Pear Cultivars

HortScience ◽  
2002 ◽  
Vol 37 (1) ◽  
pp. 194-195 ◽  
Author(s):  
Masanori Kadota ◽  
Takashi Hirano ◽  
Kiyotoshi Imizu ◽  
Yoshiji Niimi
Keyword(s):  
Root Formation ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
In Vitro Rooting ◽  
Japanese Pear ◽  
Pyrus Pyrifolia ◽  
Shoot Cultures ◽  
Pyroligneous Acid ◽  
In Vitro Shoot Proliferation

Effects of PA on in vitro shoot proliferation and root formation were investigated using shoot cultures of three Japanese pear (Pyrus pyrifolia Nakai) cultivars. PA inhibited shoot multiplication and promoted initiation and development of roots in the cultured shoots of three cultivars, resulting in increasing the proportion of rooted shoots. Chemical name used: pyroligneous acid (PA).

scholarly journals A Comparison of Huckleberry (Vaccinium pahalae) and Bilberry (Vaccinium myrtillus) Shoot Culture Performance on Different Support Systems

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 911A-911
Author(s):  
Linda J. Walker ◽  
R.B. Rogers ◽  
M.A.L. Smith
Keyword(s):  
Shoot Growth ◽  
Support Systems ◽  
Callus Formation ◽  
Shoot Culture ◽  
Shoot Proliferation ◽  
Gellan Gum ◽  
Shoot Cultures ◽  
In Vitro Cell Cultures ◽  
Solution Cultures

In vitro cell cultures of huckleberry and bilberry are sources of phytochemicals for use as food colorants and bioactive chemopreventives. Shoot cultures provide a convenient, presterile source of explants for production of callus rich in extractable pigments or other chemicals. Efficient callus formation only occurs with good-quality shoots. In this study, liquid and gelled support systems were compared in terms of their effect on shoot growth. Gellan gum-based support resulted in excellent shoot proliferation and suitable shoot length for huckleberry cultures, whereas bilberry performed slightly better on agar and agar/gellan gum support. Bilberry had a more-rapid growth rate than huckleberry. Hyperhydricity was found with the use of rafts for both species. These shoot cultures have been used as vegetative explants for callus, and have produced vivid anthocyanins in solution cultures.

Establishment of In Vitro Genebank of Colombian Cultivars of Achira (Canna edulis Ker.)

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 493c-493
Author(s):  
Luz M. Reyes ◽  
Gustavo Ligarreto ◽  
Germán Muñoz ◽  
Pilar Bravo ◽  
Edgar Valbuena
Keyword(s):  
Clonal Propagation ◽  
Root Formation ◽  
Callus Formation ◽  
Plant Genetic Resources ◽  
Shoot Formation ◽  
Growth Conditions ◽  
Degree Of Contamination ◽  
Germplasm Preservation ◽  
Aerial Roots

Clonal propagation of an Andean tuber `Achira' (Canna edulis Ker.) is currently limited to budding. A tissue-culture system to rapidly produce clonal material would be valuable for both production and germplasm preservation. Thirteen cultivars collected at the provinces of Cáqueza (Cundinamarca) and Garzón (Huila) were utilized for the establishment of meristem-tips (5 mm) under in vitro conditions. Based on protocols reported for species of Musa sp., C. indica and Elletaria cardamomun, a complete random design was implemented with 10 treatments and six replications per clone. The analysis of variance showed no significant differences between cultivars, but significant differences among treatments. The variables measured were number of shoots, aerial roots formation, roots (relative amount), callus (absent or present) and degree of etiolation. Double disinfestation protocol was used in this study in order to reduce the degree of contamination of the explants during the culture. Organogenesis was obtained for the whole cultivars, without callus formation, with treatments 9 and 10. However the best results for shoot and root formation was detected for the treatment 10. This was constituted by MS (1/2) supplemented with 0.1 ppm BAP; 0.5 ppm IBA; 3% sucrose, and 2% phytagel. The explants were grown at 26 °C with photoperiod of 16 hours and 2000 lux. After 3 weeks the shoot formation was evident, while the rooting started after 4 weeks. Subcultures were done every 3 weeks after plantlets formation. The 13 cultivars established under in vitro conditions were placed at the active genebank of the Plant Genetic Resources National Program at CORPOICA, for their conservation under low growth conditions.

Ethylene production by ectomycorrhizal fungi, Fusarium oxysporum f. sp. pini, and by aseptically synthesized ectomycorrhizae and Fusarium-infected Douglas-fir roots

1980 ◽  
Vol 26 (11) ◽  
pp. 1340-1347 ◽  
Author(s):  
J. H. Graham ◽  
R. G. Linderman
Keyword(s):  
Fusarium Oxysporum ◽  
Ectomycorrhizal Fungi ◽  
Ethylene Production ◽  
Mycorrhizal Fungi ◽  
Root Formation ◽  
Pathogenic Fungus ◽  
Douglas Fir ◽  
Cenococcum Geophilum ◽  
Host Interactions

The ectomycorrhizal fungi Cenococcum geophilum, Hebeloma crustuliniforme, and Laccaria laccata produced ethylene in vitro in modified Melin–Norkrans liquid medium only if amended with 2.5–10 mM methionine; Pisolilhus tinctorius failed to produce ethylene unless the cultures were renewed with fresh methionine-amended medium before ethylene assay. An additional 19 ectomycorrhizal fungi, plus five isolates of Fusarium oxysporum f. sp. pini, all produced ethylene in renewed and (or) nonrenewed media. Although the rates varied, ethylene production by many ectomycorrhizal fungi equaled that of Fusarium.Culture filtrates of H, crustuliniforme and L. laccata also evolved ethylene that was apparently of nonenzymatic origin.Ethylene was produced by aseptically grown Douglas-fir seedlings inoculated with C. geophilum, H. crustuliniforme, and L. laccata and appearance of ethylene coincided with the formation of mycorrhizae; production by P. tinctorius inoculated seedlings was inconsistent. Lateral root formation of Douglas-fir was stimulated by inoculation with C. geophilum, H. crustuliniforme, and L. laccata but was inhibited by P. tinctorius. Fusarium-inoculated seedlings produced more ethylene sooner than seedlings inoculated with mycorrhizal fungi. The disparity in the levels of ethylene associated with ectomycorrhiza formation compared with Fusarium infection suggests a possible differential role for ethylene in symbiotic and pathogenic fungus–host interactions.

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