Molecular characterization of group A bovine rotavirus in southeastern and central-western Brazil, 2009-2010

Rotavirus is an important cause of neonatal diarrhea in humans and several animal species, including calves. A study was conducted to examine 792 fecal samples collected from calves among 65 dairy and beef herds distributed in two of Brazil's major livestock producing regions, aiming to detect the occurrence of rotavirus and perform a molecular characterization of the rotavirus according to G and P genotypes in these regions. A total of 40 (5.05%) samples tested positive for rotavirus by the polyacrylamide gel electrophoresis (PAGE) technique. The molecular characterization was performed by multiplex semi-nested RT-PCR reactions, which indicated that the associations of genotypes circulating in herds in Brazil's southeastern region were G6P[11], G10P[11], G[-]P[5] + [11], G[-]P[6] in the state of São Paulo and G6P[11], G8P[5], G11P[11], G10P[11] in the state of Minas Gerais. In the central-western region, the genotypes G6P[5] + [11], G6P[5], G8P[-], G6P[11], G [-] P[1], G[-] P[11], and G[-] P[5] were detected in the state of Goiás, while the genotypes G6P[5], G8[P11], G6[P11], G8[P1], G8[P5], G6[P1] were circulating in herds in the state of Mato Grosso do Sul. The genotypic diversity of bovine rotavirus found in each region under study underlines the importance of characterizing the circulating samples in order to devise the most effective prophylactic measures.

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Severe diarrhea outbreak in beef calves (Bos indicus) caused by G6P[11], an emergent genotype of bovine rotavirus group A

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2014000800001 ◽

2014 ◽

Vol 34 (8) ◽

pp. 717-722 ◽

Cited By ~ 4

Author(s):

Thais N.S. Medeiros ◽

Elis Lorenzetti ◽

Alice F. Alfieri ◽

Amauri A. Alfieri

Keyword(s):

Bos Indicus ◽

Nucleotide Sequence Analysis ◽

High Morbidity ◽

Pathogenic Potential ◽

Mato Grosso ◽

Fecal Samples ◽

Beef Calves ◽

Bovine Rotavirus ◽

Group A

The episodes of diarrhea caused by neonatal bovine rotavirus group A (BoRVA) constitute one of the major health problems in the calf rearing worldwide. The main G (VP7) and P (VP4) genotypes of BoRVA strains involved in the etiology of diarrhea in calves are G6P[1], G10P[11], G6P[5], and G8P[1]. However, less frequently, other G and P genotypes have been described in BoRVA strains identified in diarrheic fecal samples of calves. This study describes the identification and molecular characterization of an emerging genotype (G6P[11]) in BoRVA strains involved in the etiology of a diarrhea outbreak in beef calves in a cattle herd of high production in extensive management system. The diarrhea outbreak, which showed high morbidity (60%) and lethality (7%) rates, occurred in calves (n= 384) Nelore (Bos indicus) up to 30-day-old from the State of Mato Grosso do Sul, Brazil. BoRVA was identified in 80% (16/20) of the fecal samples analyzed by polyacrylamide gel electrophoresis (PAGE) technique. In all PAGE-positive fecal samples were amplified products with 1,062-bp and 876-bp in the RT-PCR assays for VP7 (G type) and VP4 (VP8*) (P type) of BoRVA, respectively. The nucleotide sequence analysis of VP7 and VP4 genes of four wild-type BoRVA strains showed G6-III P[11]-III genotype/lineage. The G6P[11] genotype has been described in RVA strains of human and animal hosts, however, in calves this genotype was only identified in some cross-sectional studies and not as a single cause of diarrhea outbreaks in calves with high morbidity and lethality rates as described in this study. The monitoring of the G and P genotypes of BoRVA strains involved in diarrhea outbreaks in calves is important for both animal and public health by allowing the identification of the most frequent genotypes, the characterization of novel genotypes and to identify reassortments with genotypes described in animal and human hosts. The results of this study show the importance of the monitoring of the genotypes of BoRVA strains involved in episodes of bovine neonatal diarrhea as for characterization of frequency of occurrence and pathogenic potential of uncommon genotypes as for monitoring of the emergency of different BoRVA genotypes not included in commercial vaccines.

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Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822012000400021 ◽

2012 ◽

Vol 45 (4) ◽

pp. 520-522 ◽

Cited By ~ 3

Author(s):

Maria Sandra Moura Costa ◽

Paulo Afonso Nogueira ◽

Gleicienne Félix Magalhães ◽

Paula Taquita ◽

Luis André Mariúba ◽

...

Keyword(s):

Molecular Characterization ◽

Gel Electrophoresis ◽

Polyacrylamide Gel Electrophoresis ◽

Brazilian Amazon ◽

Rt Pcr ◽

Rotavirus A ◽

Positive Stool ◽

Group A ◽

Stool Samples

INTRODUCTION: During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon. METHODS: Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis. RESULTS: A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P[8], G3P[8], and G4P[8] genotypes. The G1P[8] genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains. CONCLUSIONS: The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon.

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Molecular characterization of hasB from an operon required for hyaluronic acid synthesis in group A streptococci. Demonstration of UDP-glucose dehydrogenase activity.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)53153-7 ◽

1993 ◽

Vol 268 (10) ◽

pp. 7118-7124

Author(s):

B.A. Dougherty ◽

I. van de Rijn

Keyword(s):

Hyaluronic Acid ◽

Dehydrogenase Activity ◽

Molecular Characterization ◽

Glucose Dehydrogenase ◽

Acid Synthesis ◽

Group A Streptococci ◽

Group A

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Molecular characterization of G and P-types bovine rotavirus strains from Goiás, Brazil: high frequency of mixed P-type infections

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762010000800014 ◽

2010 ◽

Vol 105 (8) ◽

pp. 1040-1043 ◽

Cited By ~ 12

Author(s):

Thabata Alessandra Ramos Caruzo ◽

Willia Marta Elsner Diederichsen de Brito ◽

Veridiana Munford ◽

Maria Lúcia Rácz

Keyword(s):

Molecular Characterization ◽

High Frequency ◽

Bovine Rotavirus ◽

P Type

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Detection and partial molecular characterization of Picobirnavirus in swine from the state of Minas Gerais, Brazil

Journal of Veterinary Medical Science ◽

10.1292/jvms.19-0565 ◽

2020 ◽

Vol 82 (12) ◽

pp. 1798-1801

Author(s):

Nívia Carolina Lopes ROSADO ◽

Marcus Rebouças SANTOS ◽

Laura Morais Nascimento SILVA ◽

Viviane Sisdelli ASSAO ◽

Elaine Nery ARAÚJO ◽

...

Keyword(s):

Molecular Characterization ◽

Minas Gerais ◽

The State

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Molecular characterization of the first human G15 rotavirus strain of zoonotic origin from the bovine species

Journal of General Virology ◽

10.1099/jgv.0.001581 ◽

2021 ◽

Vol 102 (4) ◽

Author(s):

Takeshi Tsugawa ◽

Yoshiki Fujii ◽

Yusuke Akane ◽

Saho Honjo ◽

Kenji Kondo ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Molecular Characterization ◽

Human Infection ◽

Potential Reservoir ◽

Group A Rotaviruses ◽

Paediatric Outpatient ◽

Group A ◽

Human Infections ◽

Bovine Species

Group A rotaviruses (RVAs) infect a wide variety of mammalian and avian species. Animals act as a potential reservoir to RVA human infections by direct virion transmission or by contributing genes to reassortants. Here, we report the molecular characterization of a rare human RVA strain Ni17-46 with a genotype G15P[14], isolated in Japan in 2017 during rotavirus surveillance in a paediatric outpatient clinic. The genome constellation of this strain was G15-P[14]-I2-R2-C2-M2-A13-N2-T9-E2-H3. This is the first report of an RVA with G15 genotype in humans, and sequencing and phylogenetic analysis results suggest that human infection with this strain has zoonotic origin from the bovine species. Given the fact that this strain was isolated from a patient with gastroenteritis and dehydration symptoms, we must take into account the virulence of this strain in humans.

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