scholarly journals Effects of intracameral brilliant blue on the corneal endothelium of swine: in vitro study

2016 ◽  
Vol 36 (8) ◽  
pp. 775-780 ◽  
Author(s):  
Mariana Terzariol ◽  
Paula S. Hünning ◽  
Gustavo Brambatti ◽  
Luciane de Albuquerque ◽  
Carolina Neumann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Anterior Chamber ◽  
Corneal Endothelium ◽  
Brilliant Blue ◽  
Ultrastructural Changes ◽  
Blue Dye ◽  
Endothelium Cells ◽  
The Right ◽  
Scanning Electron

Abstract: The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue±) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution injection and the cornea was excised too. All the corneas were evaluated by scanning electron microscopy to evaluate the changes on the endothelium caused by the brilliant blue dye. There were no significant differences between the right corneal endothelium cells and the left corneal endothelium cells with scanning electron microscopy after intracameral use of 0.05% brilliant blue dye. The 0.05% brilliant blue dye concentration did not cause deleterious effects for the swine corneal endothelium after intracameral use and can be a choice for safe staining of the anterior capsule of the lens in cataract surgery.

scholarly journals Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium

2020 ◽  
Vol 41 (6supl2) ◽  
pp. 3155-3164
Author(s):  
Luciane de Albuquerque ◽  
◽  
Anita Marchionatti Pigatto ◽  
João Antonio Tadeu Pigatto ◽  
◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Corneal Endothelium ◽  
Cell Loss ◽  
Average Cell ◽  
Hypothermic Preservation ◽  
Scanning Electron ◽  
Preservation Medium

The objective of this study was evaluate the maintenance of the corneal endothelium of horses in cold EUSOL-C® preservation medium over different periods (seven and 14 days) using scanning electron microscopy. A total of 20 pairs of eyes from horses were analysed. The corneas were divided into four groups of 10 corneas each (G1, G2, G3 and G4): G1 - the samples were kept in the preservation medium for seven days; G3 - the samples were kept in the preservation medium for for 14 days; G2 and G4 were formed by the control corneal buttons of G1 and G3, respectively. The average cell loss observed in G1 was 7.62%, in G2 it was 7.04%, in G3 9.12% and in G4 7.16%. No statistically significant differences were observed between the four groups. It was concluded that the Eusol-C® hypothermic preservation medium provided satisfactory preservation of the corneal endothelium in equine species for up to 14 days.

Extra-alveolar veins are contiguous with, and leak fluid into, periarterial cuffs in rabbit lungs

1991 ◽  
Vol 71 (4) ◽  
pp. 1606-1613 ◽  
Author(s):  
D. L. Luchtel ◽  
L. Embree ◽  
R. Guest ◽  
R. K. Albert
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Interstitial Fluid ◽  
Blue Dye ◽  
Alveolar Pressure ◽  
Vessel Lumen ◽  
Freeze Dried ◽  
Orange G ◽  
Pulmonary Arterial ◽  
Scanning Electron

We previously observed physiological evidence that arterial and venous extra-alveolar vessels shared a common interstitial space. The purpose of the present investigation was to determine the site of this continuity to improve our understanding of interstitial fluid movement in the lung. Orange G and Evans blue dyes were added to the arterial and venous reservoirs, respectively, of excised rabbit lungs as they were placed 20 cmH2O into zone 1 (pulmonary arterial and venous pressures = 5 cmH2O, alveolar pressure = 25 cmH2O). After 10 s or 4 h the lungs were fixed by immersion in liquid N2, freeze-dried, cut into 5-mm serial slices, and examined by light macroscopy. Serial sections of 0.25–0.5 mm were subsequently examined by scanning electron microscopy. In the animals subjected to the zone 1 stress for 4 h, arterial and venous extra-alveolar vessels were surrounded by cuffs of edema. The edema ratio (cuff area divided by vessel lumen area) was greater around arteries than veins and decreased with increasing vessel size. Periarterial cuffs usually contained orange dye and frequently contained both orange and blue dye. Lymphatics containing orange or blue dye were frequently seen in periarterial cuffs. Scanning electron microscopy demonstrated that extra-alveolar veins of approximately 100 microns diameter were anatomically contiguous with arterial extra-alveolar vessel cuffs. In rabbit lungs, both arterial and venous extra-alveolar vessels (and/or alveolar corner vessels) leak fluid into perivascular cuffs surrounding arterial extra-alveolar vessels, and lymphatics located in the periarterial cuff contain fluid that originates from both the arterial and venous extra-alveolar vessels.

scholarly journals Effect of denture surface glazing on denture plaque formation

2005 ◽  
Vol 16 (2) ◽  
pp. 129-134 ◽  
Author(s):  
Newton Sesma ◽  
Dalva Cruz Laganá ◽  
Susana Morimoto ◽  
Carlos Gil
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bacterial Colonization ◽  
Plaque Formation ◽  
The Other ◽  
Bacterial Plaque ◽  
The Right ◽  
Clinical Experiment ◽  
Scanning Electron

This study evaluated, in vivo, the efficacy of a denture glazing material (Palaseal) in modifying plaque colonization of dentures. Ten subjects were selected and received maxillary temporary partial removable dentures, with complete acrylic palatal coverage. The right half of the fitting surface of the denture bases were glazed with Palaseal, whereas the other half was not glazed. One month after insertion, two fragments of the resin base of all dentures were removed (one from the glazed side and another from the non-glazed side). These samples were prepared and examined by scanning electron microscopy. Three months after insertion, other fragments were obtained and analyzed. Microscopic observation at 1 month revealed that, for all patients, the plaque film was thinner on the treated side in comparison to the non-treated side. However, at the 3-month evaluation, some areas of the glaze showed cracking, and both glazed and non-glazed sides were covered by a dense bacterial plaque film. In conclusion, the findings of this clinical experiment showed that glazing denture's fitting surface did not prevent bacterial colonization, but favored plaque removal while the glaze layer remained intact. After three months, glaze cracks created microretentive areas that increased plaque accumulation.

Morphometric analysis of the corneal endothelium of Yacare caiman (Caiman yacare) using scanning electron microscopy

2004 ◽  
Vol 7 (3) ◽  
pp. 205-208 ◽  
Author(s):  
Joao A. T. Pigatto ◽  
Marcos C. Andrade ◽  
Jose Luiz Laus ◽  
Jaime M. Santos ◽  
Dennis E. Brooks ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphometric Analysis ◽  
Corneal Endothelium ◽  
Scanning Electron

CORNEAL ENDOTHELIUM OF THE MAGELLANIC PENGUIN (SPHENISCUS MAGELLANICUS) BY SCANNING ELECTRON MICROSCOPY

2005 ◽  
Vol 36 (4) ◽  
pp. 702-705 ◽  
Author(s):  
João A. T. Pigatto ◽  
José L. Laus ◽  
Jaime M. Santos ◽  
Cristine Cerva ◽  
Luciana S. Cunha ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Corneal Endothelium ◽  
Magellanic Penguin ◽  
Spheniscus Magellanicus ◽  
Scanning Electron

scholarly journals Intraocular Lens Opacification following Intracameral Injection of Recombinant Tissue Plasminogen Activator to Treat Inflammatory Membranes after Cataract Surgery

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Simon S. M. Fung ◽  
Evripidis Sykakis ◽  
Niaz M. Islam ◽  
Hadi J. Zambarakji ◽  
Ramin Khoramnia ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Cataract Surgery ◽  
Anterior Chamber ◽  
Plasminogen Activator ◽  
Recombinant Tissue Plasminogen Activator ◽  
X Ray ◽  
Tissue Plasminogen ◽  
Scanning Electron

Purpose. To report 7 cases of intraocular lens (IOL) opacification following treatment of postoperative anterior chamber fibrin with recombinant tissue plasminogen activator (rtPA) after cataract surgery. Methods. Retrospective case series of 7 eyes in 7 patients who developed IOL opacification after receiving rtPA for anterior chamber inflammatory membrane formation resulting from phacoemulsification cataract surgery. Three explanted IOLs were investigated with light microscopy, histochemical analysis, scanning electron microscopy, and X-ray spectrometry. Results. All patients underwent uncomplicated cataract surgery and posterior chamber hydrophilic IOL implantation. Anterior chamber inflammatory membranes developed between 1 and 4 weeks of surgery and were treated with intracameral rtPA. IOL opacification was noted between 4 weeks and 6 years after rtPA treatment with reduced visual acuity, and IOL exchange was carried out in 3 patients. Light microscopy evaluation revealed diffuse fine granular deposits on the anterior surface/subsurface of IOL optic that stained positive for calcium salts. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometry (EDS) confirmed the presence of calcium and phosphate on the IOL. Conclusions. Intracameral rtPA, though rapidly effective in the treatment of anterior chamber inflammatory membranes following cataract surgery, may be associated with IOL opacification.

scholarly journals The bone ultrastructural changes on combat trauma condition represented by scanning electron microscopy

Morphologia ◽  
2018 ◽  
Vol 1 (1) ◽  
pp. 7-13
Author(s):  
N. O. Borzykh ◽  
S. S. Strafun ◽  
S. I. Savosko ◽  
O. M. Makarenko ◽  
A. A. Laksha
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Combat Trauma ◽  
Ultrastructural Changes ◽  
Scanning Electron
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