scholarly journals Evaluation of equine (Equus cabbalus) corneal endothelium stored in EUSOL-C® preservation medium

2020 ◽  
Vol 41 (6supl2) ◽  
pp. 3155-3164
Author(s):  
Luciane de Albuquerque ◽  
◽  
Anita Marchionatti Pigatto ◽  
João Antonio Tadeu Pigatto ◽  
◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Corneal Endothelium ◽  
Cell Loss ◽  
Average Cell ◽  
Hypothermic Preservation ◽  
Scanning Electron ◽  
Preservation Medium

The objective of this study was evaluate the maintenance of the corneal endothelium of horses in cold EUSOL-C® preservation medium over different periods (seven and 14 days) using scanning electron microscopy. A total of 20 pairs of eyes from horses were analysed. The corneas were divided into four groups of 10 corneas each (G1, G2, G3 and G4): G1 - the samples were kept in the preservation medium for seven days; G3 - the samples were kept in the preservation medium for for 14 days; G2 and G4 were formed by the control corneal buttons of G1 and G3, respectively. The average cell loss observed in G1 was 7.62%, in G2 it was 7.04%, in G3 9.12% and in G4 7.16%. No statistically significant differences were observed between the four groups. It was concluded that the Eusol-C® hypothermic preservation medium provided satisfactory preservation of the corneal endothelium in equine species for up to 14 days.

scholarly journals Scanning electron microscopy of the corneal endothelium of ostrich

2009 ◽  
Vol 39 (3) ◽  
pp. 926-929 ◽  
Author(s):  
João Antonio Tadeu Pigatto ◽  
Angela Aguiar Franzen ◽  
Fabiana Quartiero Pereira ◽  
Ana Carolina da Veiga Rodarte de Almeida ◽  
José Luis Laus ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Endothelial Cells ◽  
Cell Density ◽  
Coefficient Of Variation ◽  
Corneal Endothelium ◽  
Cell Area ◽  
Corneal Endothelial Cells ◽  
Average Cell ◽  
Scanning Electron

The aim of this study was to examine the endothelial surface morphology and perform a morphometric analysis of the corneal endothelial cells of ostrich (Struthio camelus) using scanning electron microscopy. Polygonality, mean cell area, cell density and coefficient of variation of mean cell area were analyzed. The normal corneal endothelium consisted of polygonal cells of uniform size and shape with few interdigitations of the cell borders. Microvilli appeared as protusions on the cellular surface. The average cell area was 269±18µm² and the endothelial cell density was 3717±240cells mm-2. The coefficient of variation of the cell area was 0.06, and the percentage of hexagonal cells was 75%. The parameters evaluated did not differ significantly between the right and the left eye from the same ostrich. The results of this study showed that the ostrich corneal endothelial cells appear quite similar to those of the other vertebrates.

Morphometric analysis of the corneal endothelium of Yacare caiman (Caiman yacare) using scanning electron microscopy

2004 ◽  
Vol 7 (3) ◽  
pp. 205-208 ◽  
Author(s):  
Joao A. T. Pigatto ◽  
Marcos C. Andrade ◽  
Jose Luiz Laus ◽  
Jaime M. Santos ◽  
Dennis E. Brooks ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphometric Analysis ◽  
Corneal Endothelium ◽  
Scanning Electron

CORNEAL ENDOTHELIUM OF THE MAGELLANIC PENGUIN (SPHENISCUS MAGELLANICUS) BY SCANNING ELECTRON MICROSCOPY

2005 ◽  
Vol 36 (4) ◽  
pp. 702-705 ◽  
Author(s):  
João A. T. Pigatto ◽  
José L. Laus ◽  
Jaime M. Santos ◽  
Cristine Cerva ◽  
Luciana S. Cunha ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Corneal Endothelium ◽  
Magellanic Penguin ◽  
Spheniscus Magellanicus ◽  
Scanning Electron

Cochlear Hair Cell Loss in Ears with Cholesteatomas Scanning Electron Microscopy Study

1988 ◽  
Vol 97 (1) ◽  
pp. 78-82 ◽  
Author(s):  
Richard A. Chole ◽  
Maggie Chiu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Hair Cell ◽  
Hair Cells ◽  
Cell Loss ◽  
Outer Hair Cells ◽  
Mongolian Gerbils ◽  
Hair Cell Loss ◽  
Cochlear Hair Cell ◽  
Scanning Electron

Cochleas from 16 Mongolian gerbils with spontaneous aural cholesteatomas, and four of similar age without cholesteatomas, were examined by scanning electron microscopy to quantify cochlear hair cell loss. Loss of hair cell stereocilia was found in all ears with cholesteatomas and was increased when compared with uninvolved ears from animals of similar age. The hair cell loss assorted with gerbilline cholesteatomas appeared to be most marked in the middle turn of the cochlea and increased in severity with increasing size of the cholesteatomas. Outer hair cells were affected more than inner hair cells. Inner and outer hair cell loss was not significantly different infected cholesteatomas versus sterile cholesteatomas. The greater damage to hair cels at the middle turn compared to the basal turn suggests that these losses may be the result of some agent acting through the cochlear wall rather than through the round window.

scholarly journals Effects of intracameral brilliant blue on the corneal endothelium of swine: in vitro study

2016 ◽  
Vol 36 (8) ◽  
pp. 775-780 ◽  
Author(s):  
Mariana Terzariol ◽  
Paula S. Hünning ◽  
Gustavo Brambatti ◽  
Luciane de Albuquerque ◽  
Carolina Neumann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Anterior Chamber ◽  
Corneal Endothelium ◽  
Brilliant Blue ◽  
Ultrastructural Changes ◽  
Blue Dye ◽  
Endothelium Cells ◽  
The Right ◽  
Scanning Electron

Abstract: The aim was to investigate the ultrastructural changes in the corneal endothelium of pigs induced by intracameral 0.05% brilliant blue. Twenty swine corneas were separated into two groups, the right eye bulbs (control group) and the left eye bulbs (experimental group) of the same animal. All the eye bulbs were evaluated with specular microscopy. The cornea of the right eye bulbs was excised and in the left eye bulbs 0.2ml of 0.05% brilliant blue vital dye (OPTH-blue±) was injected into the anterior chamber, where it remained for one minute. Then the anterior chamber was cleaned with a balanced salt solution injection and the cornea was excised too. All the corneas were evaluated by scanning electron microscopy to evaluate the changes on the endothelium caused by the brilliant blue dye. There were no significant differences between the right corneal endothelium cells and the left corneal endothelium cells with scanning electron microscopy after intracameral use of 0.05% brilliant blue dye. The 0.05% brilliant blue dye concentration did not cause deleterious effects for the swine corneal endothelium after intracameral use and can be a choice for safe staining of the anterior capsule of the lens in cataract surgery.

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