scholarly journals A comparative survey between non-systemic Salmonella spp. (paratyphoid group) and systemic Salmonella Pullorum and S. Gallinarum with a focus on virulence genes

2017 ◽  
Vol 37 (10) ◽  
pp. 1064-1068 ◽  
Author(s):  
Claudete S. Astolfi-Ferreira ◽  
Marcelo R.S. Pequini ◽  
Luis F.N. Nuñez ◽  
Silvana H. Santander Parra ◽  
Ruy Chacon ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Virulence Gene ◽  
Gene Profile ◽  
Salmonella Spp ◽  
Salmonella Pullorum ◽  
Comparative Survey ◽  
Virulence Gene Profile

ABSTRACT: A comparative survey between non-systemic (paratyphoid Salmonellae) and systemic (S. Pullorum and S. Gallinarum) Salmonella strains was performed to produce a virulence gene profile for differentiation among the groups. The following virulence genes were evaluated: invA, spvC, sefC, pefA, fimY, sopB, sopE1, stn and avrA. There are substantial differences among paratyphoid Salmonellae, S. Pullorum, and S. Gallinarum regarding the genes sefC, spvC, sopE1 and avrA. A higher frequency of sefC, spvC, sopE1 and avrA genes were detected in S. Gallinarum and S. Pullorum when compared with strains from the paratyphoid group of Salmonella. These results may be useful for differentiating among different groups and serotypes.

Serological diversity, molecular characterisation and antimicrobial sensitivity of avian pathogenic Escherichia coli (APEC) isolates from broiler chickens in Kashmir, India

2019 ◽  
Vol 59 (2) ◽  
pp. 338
Author(s):  
S. N. Magray ◽  
S. A. Wani ◽  
Z. A. Kashoo ◽  
M. A. Bhat ◽  
S. Adil ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Gene Profile ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli ◽  
Significant Difference ◽  
Virulence Gene Profile

The present study has determined the serological diversity, virulence-gene profile and in vitro antibiogram of avian pathogenic Escherichia coli (APEC) isolates from broiler chickens in India suspected to have died of colibacillosis. The virulence-gene profile of APEC was compared with that of the Escherichia coli isolates from faeces of apparently healthy chickens, called avian faecal E. coli (AFEC). In total, 90 representative isolates of APEC and 63 isolates of AFEC were investigated in the present study. The APEC were typed into 19 serogroups, while some isolates were rough and could not be typed. Most prevalent serogroup was O2 (24.44%). Among the eight virulence genes studied, the prevalence of seven genes (iss, iucD, tsh, cva/cvi, irp2, papC and vat) was significantly higher in APEC than in AFEC isolates. However, there was no significant difference between APEC and AFEC isolates for possession of astA gene. The most frequent gene detected among the two groups of organisms was iss, which was present in 98.88% and 44.44% of APEC and AFEC isolates respectively. The in vitro antibiogram showed that the majority (96.6%) of APEC isolates were resistant to tetracycline, while 82.2% were resistant to cephalexin, 78.8% to cotrimoxazole, 68.8% to streptomycin and 63.3% to ampicillin. However, most of them (84.45%) were sensitive to gentamicin. Thus, it is concluded that APEC from the broiler chickens carried putative virulence genes that attributed to their pathogenicity. Furthermore, the majority of APEC isolates were found to be multi-drug resistant, which, in addition to leading treatment failures in poultry, poses a public health threat.

Genetic diversity, phylogroup distribution and virulence gene profile of pks positive Escherichia coli colonizing human intestinal polyps

2017 ◽  
Vol 112 ◽  
pp. 274-278 ◽  
Author(s):  
Meysam Sarshar ◽  
Daniela Scribano ◽  
Massimiliano Marazzato ◽  
Cecilia Ambrosi ◽  
Maria Rita Aprea ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Virulence Gene ◽  
Gene Profile ◽  
Intestinal Polyps ◽  
Virulence Gene Profile

Molecular detection of biofilm, virulence and antimicrobial resistance associated genes of Salmonella serovars isolated from pig and chicken of Mizoram, India

2019 ◽  
Author(s):  
S. Chakraborty ◽  
P. Roychoudhury ◽  
I. Samanta ◽  
P. K. Subudhi ◽  
Lalhruaipuii Lalhruaipuii ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Virulence Gene ◽  
Microtiter Plate ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
Microtiter Plate Assay ◽  
Antimicrobial Sensitivity ◽  
Salmonella Infections

Salmonella has emerged as one of the most important food-borne pathogens for humans as well as animals and the ability of biofilm formation by these bacteria has further aided their survival in unfavorable environment. Characterization of these biofilm producing bacteria isolated from pigs and chicken may lead to formulation of strategies for prevention and control of Salmonella infections. Therefore, the present study was conducted to isolate Salmonella from pigs and poultry of Mizoram, determine their biofilm producing ability by phenotypic and genotypic methods along with their virulence and antimicrobial resistance properties. A total of 15 Salmonella spp. (pig=9, poultry=6) was isolated from 100 faecal samples from pigs and 50 cloacal swabs from poultry and biofilm producing ability of the isolates was determined by microtiter plate assay. A total of 10 (66.67%) isolates were found to be biofilm producer. All the biofilm producing bacterial isolates were investigated for antimicrobial sensitivity and distribution of selected biofilm associated genes (csgA, csgD and adrA), virulence genes (invA, stn and sefA) and antimicrobial resistance (AMR) genes (blaTEM, blaSHV and blaCTX-M). The most prevalent resistance was found against ceftazidime (80%), ceftriaxone (80%), cefixime (70%), cefotaxime (70%), gentamicin (70%), cotrimoxazole (60%) and ampicillin (60%). A total of 7 (70%) isolates were resistant to at least three different classes of antimicrobial agents and considered as multidrug resistant. All the isolates were positive for adrA (100%) but negative for csgA and csgD genes. The most frequent virulence gene was invA (100%) and stn (100%). Among the AMR genes, blaTEM (60%) was found to be the major AMR determinants. Moreover, a total of 7 Salmonella isolates were positive for at least one of t biofilm associated genes, virulence genes and AMR genes.

scholarly journals Prevalence of Campylobacter species in milk and milk products, their virulence gene profile and antibiogram

2015 ◽  
Vol 8 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Shivani Modi ◽  
M. N. Brahmbhatt ◽  
Y. A. Chatur ◽  
J. B. Nayak
Keyword(s):  
Virulence Gene ◽  
Gene Profile ◽  
Milk Products ◽  
Campylobacter Species ◽  
Virulence Gene Profile

scholarly journals Molecular Characterization Of Pathogenic Salmonella Spp From Raw Beef In Karachi, Pakistan

Antibiotics ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 73
Author(s):  
Muhammad Altaf Hussain ◽  
Wan Wang ◽  
Changbao Sun ◽  
Liya Gu ◽  
Zhijing Liu ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Salmonella Enteritidis ◽  
Virulence Genes ◽  
Salmonella Spp ◽  
Beta Lactamases ◽  
Minced Beef ◽  
Salmonella Pullorum ◽  
Beef Muscles ◽  
Butcher Shops

The aim of the present study was to estimate the prevalence of Salmonella and investigate the dominant serovars distribution in raw beef and to screen the isolated serovars for the prescense of beta-lactamases and virulence genes. A total of 150 samples of raw beef sold at butcher shops (n = 75) and supermarkets (n = 75) in Karachi city were collected (50 samples each from muscles, lymph nodes, and minced beef). The samples were cultured according to the ISO-6579-1guidlines. The overall prevalence of Salmonella strains was found to be 21.34%. A total of 56 isolates of Salmonella belonging to four serogroups (Salmonella Pullorum, Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Choleraesuis) were isolated from beef muscles (12%), lymph nodes (24%) and minced beef (28%) samples collected from butcher shops (av. 21.34%). No Salmonella was detected in beef samples collected from supermarkets. S. Enteritidis contamination was highest (37.5%), followed by S. Choleraesuis (30.4%), S. Pullorum (19.6%) and S. Typhimurium (12.5 %). Antibiotic susceptibility testing revealed that Salmonella isolates were highly resistant to Oxytetracycline (90%), Ampicillin (90.5%), Amoxicillin (81.1%), Tetracycline (76%), Neomycin, (79.8%) and Ciprofloxacin (61.4%). The Salmonella isolates examined were more susceptible to the Cephalosporin antibiotics such as Cefixime (43.2%), Cefepime (48.2) and Cefoxitin (49.8%). PCR based screening of blaTEM, blaCTX-M and blaSHV revealed that blaCTX-M and blaTEM were the dominant resistant genes in S. Enteritidis and S. Typhimurium followed by S. Pullorum and S. Choleraesuis whereas blaSHV was the least detected beta-lactamase in Salmonella isolates. Virulence genes screening revealed that at least five genes were present in all the serovars, highest being present in S. Enteritidis (12/17) and S. Typhimurium (12/17). S. Cholerasuis (5/17) carried the least number of virulence genes followed by S. Pullorum (6/17). The present data suggest that beef samples from butcher shops of Karachi city are heavily contaminated with MDR Salmonella. The presence of resistance and virulence genes in MDR strains of Salmonella may play a significant role in transmission and development of Salmonella infection in humans.

scholarly journals Virulence Gene Profile and Serogroups of STEC in Diarrhoeic Buffalo Calves

2020 ◽  
Vol 9 (9) ◽  
pp. 2696-2703
Author(s):  
M. Srivani ◽  
Y. Narasimha Reddy ◽  
K.V. Subramanyam ◽  
M. Lakshman ◽  
T. Srinivasa Rao ◽  
...  
Keyword(s):  
Virulence Gene ◽  
Gene Profile ◽  
Buffalo Calves ◽  
Virulence Gene Profile
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