scholarly journals Detection of cheese whey in raw milk preserved with bronopol® through high performance liquid chromatography

2011 ◽  
Vol 63 (6) ◽  
pp. 1553-1558 ◽  
Author(s):  
M.M. Lasmar ◽  
M.O. Leite ◽  
L.M. Fonseca ◽  
M.R. Souza ◽  
M.M.O.P. Cerqueira ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Cheese Whey ◽  
Raw Milk ◽  
The Other ◽  
Random Design ◽  
Non Parametric

High performance liquid chromatography was used in order to detect cheese whey in samples of raw milk preserved with Bronopol®. Six samples were collected and divided in 45 aliquots of 40mL. From these, 15 were used as control and stored frozen, 15 were added with Bronopol® and stored at 7ºC, and the other 15 were added with Bronopol® and stored at 30ºC. In all groups, five levels of cheese whey addition (0, 2, 5, 10, and 20%) were tested. The samples were submitted to high performance liquid chromatography on the 2nd, 4th, and 8th days of storage. A completely random design was used, following the factorial scheme (5x3x3) and the results were compared through the non-parametric Kruskal-Wallis test. There was no difference among the treatments (P>0.05), which allows the conclusion that raw milk preserved with Bronopol® may be used for the determination of cheese whey addition in milk through high performance liquid chromatography.

scholarly journals Short communication: Determination of lactoferrin in Feta cheese whey with reversed-phase high-performance liquid chromatography

2014 ◽  
Vol 97 (8) ◽  
pp. 4832-4837 ◽  
Author(s):  
E. Tsakali ◽  
K. Petrotos ◽  
A. Chatzilazarou ◽  
K. Stamatopoulos ◽  
A.G. D’Alessandro ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Short Communication ◽  
Cheese Whey ◽  
Reversed Phase ◽  
Feta Cheese

scholarly journals Comparative analysis of the effectiveness of analytical methods for the determination of aflatoxins in milk and dairy products (review information)

2020 ◽  
pp. 150-157
Author(s):  
S. Senin ◽  
V. Danchuk ◽  
S. Midyk ◽  
V. Ushkalov ◽  
O. Iakubchak
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
Analytical Methods ◽  
Dairy Products ◽  
High Performance ◽  
Raw Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Milk And Dairy Products

The dairy industry of Ukraine is developing dynamically, its needs for the quality of raw materials are growing significantly. Detection of mycotoxins in raw milk is one of the main indicators of its safety. The high degree of toxicity of mycotoxins is a threat to the health of the lactating animal, so a large number of them are excreted in milk. If we talk about ruminants, the vast majority of mycotoxins are utilized by microorganisms of the pancreas, which does not occur in monogastric animals, so the list of mycotoxins in their milk can be much wider than the secretion of mammalian mammals. To date, the maximum permissible levels (MRLs) of mycotoxins in raw milk and dairy products have been established. Thus, a comprehensive determination of the content of mycotoxins in the secretion of the breast has not only technological but also important diagnostic value. Milk sample preparation is the most important step in the determination of mycotoxins and consists of sampling, extraction and purification from impurities. For the extraction of aflatoxins, the method of liquid extraction with acetonitrile or chloroform is used. Purification of extracts is carried out on immunoaffinity columns, cartridges with special sorbents or using certain manufacturers (MycoSep®).Enzyme-linked immunosorbent assay and high-performance liquid chromatography with fluorescence detection are used to determine aflatoxin B1 and M1 in raw milk of cows. However, all these methods have a number of disadvantages, namely: long and expensive sample preparation and insufficiently high selectivity. Currently, the complex determination of mycotoxins in various matrices by high-performance liquid chromatography with mass spectrometric detection (LC-MS/MS) and the use of modified QuEChERS sample preparation is gaining popularity. The advantage of this technique is the combination of faster and cheaper sample preparation of QuEChERS samples with highly selective LC-MS/MS chromatography. Key words: mycotoxins, raw milk, analytical methods, QuEChERS.

Evaluation of three minicolumn procedures for measuring hemoglobin A1.

1982 ◽  
Vol 28 (8) ◽  
pp. 1775-1778 ◽  
Author(s):  
G T Hammons ◽  
K Junger ◽  
J M McDonald ◽  
J H Ladenson
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
The Other ◽  
Diabetic Patients ◽  
Hemoglobin A1 ◽  
Assay Precision ◽  
Column Chromatographic

Abstract We have evaluated three commercially available column-chromatographic methods (Isolab, Helena, and Bio-Rad) for the determination of "fast" hemoglobin (HbA1). All three methods correlated with HbA1c measurements by "high-performance" liquid chromatography for 121 samples from diabetic patients, with the Isolab method showing the highest correlation (r = 0.921). The Isolab and Helena methods gave results that were linear with proportions of HbA1 as great as 30%; results by the Bio-Rad method were slightly nonlinear at values greater than 15%. The Isolab method showed better within- and between-assay precision (CV) than the other two methods and was considered the simplest to perform by each of four different technologists. We recommend use of the Isolab method over the other two tested and believe that this procedure will be valuable for monitoring long-term glycemic control in diabetic patients.

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