scholarly journals Oxidized tissue proteins after intestinal reperfusion injury in rats

2005 ◽  
Vol 20 (6) ◽  
pp. 434-436 ◽  
Author(s):  
Alberto Schanaider ◽  
Vinícius José Martinho Toledo Menezes ◽  
Aline Cury Borchardt ◽  
Pedro Lagerblad de Oliveira ◽  
Kalil Madi
Keyword(s):  
Oxidative Stress ◽  
Reperfusion Injury ◽  
Intestinal Ischemia ◽  
Ischemia And Reperfusion ◽  
Ischemia And Reperfusion Injury ◽  
Group Iii ◽  
Protein Levels ◽  
Group I ◽  
Carbonyl Protein ◽  
Male Wistar Rats

PURPOSE: To analyse if the carbonyl proteins measurement could be validated as a method that allows the identification of an intestinal oxidative stress after ischemia and reperfusion injury. METHODS: Twenty-five male Wistar rats (n =21) weighting 200 to 250g were divided into three groups. Group I - control (n = 10). Group II - sham (n = 5) and Group III (n = 10) subjected to 60 minutes of intestinal ischemia and equal period of reperfusion. For this purpose it was clamped the superior mesenteric artery in its distal third. Histological changes and carbonyl protein levels were determined in the samples of all groups. In group III, samples of both normal and reperfused ileal segment were studied. RESULTS: All the reperfused segments showed mucosal and submucosal swelling and inflammatory infiltrate of the lamina propria. Levels of carbonyl protein rose in group III, including in the non-ischemic segments. The sensitivity and specificity of the carbonyl protein tissue levels were respectively 94% and 88%. CONCLUSION: The carbonyl protein method is a useful biologic marker of oxidative stress after the phenomenon of intestinal ischemia and reperfusion in rats. It was also noteworthy that the effects of oxidative stress could be seen far from the locus of the primary injury.

scholarly journals Effect of concurrent exposure of toxic concentrations of lead and endosulfan on oxidative stress indices in rats

2021 ◽  
Vol 10 (3) ◽  
pp. 192-195
Author(s):  
Ranganathan V ◽  
◽  
Malik JK ◽  
Rao GS ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reduced Glutathione ◽  
Group Iv ◽  
Male Rats ◽  
Technical Grade ◽  
Stress Indices ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
Toxic Concentrations

The effect of concurrent exposure of toxic concentrations of lead and endosulfan were evaluated on oxidative stress parameters in male wistar rats. Group I served as untreated control whereas Group II received drinking water containing lead as lead acetate @1000 ppm (Pb1000). Group III was exposed to feed containing technical grade endosulfan @ 100 ppm (E100). Group IV was exposed to Pb (1000) +E (100). All the treatments were given daily for 28 days. Combination of lead and endosulfan modified the indices of oxidative stress in the parameters such as lipid peroxidation, reduced glutathione, superoxide dismutase and catalase in rats as compared to their individual compounds. The results suggest that the combination of these individual compounds may have the potential to modify oxidative stress produced by single compounds in male rats

scholarly journals Quantitative characterization of changes in the cardiac mitochondrial proteome during anesthetic preconditioning and ischemia

2013 ◽  
Vol 45 (5) ◽  
pp. 163-170 ◽  
Author(s):  
Martin Bienengraeber ◽  
Molly Pellitteri-Hahn ◽  
Naoyuki Hirata ◽  
Tesfaye M. Baye ◽  
Zeljko J. Bosnjak ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Mitochondrial Protein ◽  
Ischemia And Reperfusion ◽  
Ischemia And Reperfusion Injury ◽  
Functional Changes ◽  
Mass Spectral ◽  
Mitochondrial Proteome ◽  
Protein Levels ◽  
Before And After ◽  
Quantitative Changes

Changes in mitochondrial bioenergetics have been proposed to be critical for triggering and effecting anesthetic-induced preconditioning (APC) against cardiac ischemia and reperfusion injury. The objective of this study was to analyze changes in mitochondrial protein levels and link those changes to potential functional changes. A 18O-labeling method was applied for relative comparison of cardiac mitochondrial samples from control and isoflurane exposed rats before and after ischemia and reperfusion. Wistar rats were exposed to isoflurane for 30 min (APC) or did not receive the anesthetic (control). Rats were subjected to 30 min coronary occlusion and 15 min reperfusion without (ischemia) or after APC (ischemia + APC). The following comparisons were made: control vs. APC, control vs. ischemia, and APC vs. ischemia + APC. Proteins were analyzed by liquid chromatography-mass spectrometry. A total of 98 proteins currently annotated as mitochondrial proteins in the UniProt database were positively identified from three replicate experiments. Most of the changes during APC and ischemia occur in complexes of the electron transport chain. Overall, fewer changes in ETC complexes were found when comparing APC with APC + ischemia than when comparing control and ischemia. This corresponds to the preservation of bioenergetics due to APC after ischemia and reperfusion as indicated by preserved ATP level and generation. APC itself induced changes in complex I, but those changes were not correlated with activity changes in mitochondria after APC. Thus, a proteomic mass spectral approach does not only assess quantitative changes without prior knowledge of proteins, but also allows insight into the mechanisms of ischemia and reperfusion injury and APC.

Treatment with antithymocyte globulin ameliorates intestinal ischemia and reperfusion injury in mice

Surgery ◽  
2012 ◽  
Vol 152 (5) ◽  
pp. 843-850 ◽  
Author(s):  
Melissa J. Watson ◽  
Bibo Ke ◽  
Xiu-Da Shen ◽  
Feng Gao ◽  
Ronald W. Busuttil ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Antithymocyte Globulin ◽  
Intestinal Ischemia ◽  
Ischemia And Reperfusion ◽  
Ischemia And Reperfusion Injury
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