Immunohistochemical evaluation of the inflammatory response in periodontal disease

In order to contribute to the knowledge of the pathogenesis of periodontal disease, an immunohistochemical analysis of the density of inflammatory mononucleated cells and the number of dendritic cells was performed using anti-CD4, anti-CD20, anti-CD25, anti-CD68 and anti-protein S-100 antibodies in 17 cases of chronic gingivitis (CG) and 25 of chronic periodontitis (CP). The CD4+ and CD68+ cells exhibited a diffuse distribution in the connective tissue. CD20+ cell distribution was predominantly in groups and the CD25+ cells exhibited a diffuse or focal distribution. The S-100+ cells were identified in the epithelium and the lamina propria, exhibiting distinct morphology and number. The statistical analysis showed no significant differences (p>0.05) between CG and CP regarding the density of the CD4+ and CD20+ cells and the number of S-100+ cells. However, significant differences (p<0.05) were found between the groups in the density of CD25+ and CD68+ cells . The density of macrophages was greater in CG and the level of cellular activation of the lymphocyte infiltrate was greater in CP. No differences were detected between the aforementioned conditions regarding the density of the T and B lymphocytes and to the number of the dendritic cells.

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Immunohistochemical evaluation of CD25+ cell expression in the progression of periodontal disease

Brazilian Dental Journal ◽

10.1590/s0103-64402012000400003 ◽

2012 ◽

Vol 23 (4) ◽

pp. 322-327 ◽

Cited By ~ 5

Author(s):

Ruthinéia Diogénes Alves Uchoa Lins ◽

Pollianna Muniz Alves ◽

Gustavo Pina Godoy ◽

Ericka Janine Dantas Silveira ◽

Lélia Maria Guedes Queiroz ◽

...

Keyword(s):

Connective Tissue ◽

Periodontal Disease ◽

Chronic Periodontitis ◽

Cellular Activation ◽

Cellular Density ◽

Cellular Pattern ◽

Immunohistochemical Profile ◽

Cell Expression ◽

Diffuse Distribution

It was assessed the immunohistochemical profile of CD25+ cells in cases of chronic gingivitis (CG) and chronic periodontitis (CP). Immunohistochemistry was carried out using streptoavidin-biotin complex and anti-CD25 antibody in 17 cases of CG and 25 cases of CP. Sixteen cases (94.1%) of CG were immunopositive. CD25 was focally expressed in 50% of the sample and diffusely expressed in 25%. The stained cells were localized not only beneath the epithelium, but also far from it. In relation to the cellular density quantification of CD25+ cells, score ++ was the most common. Concerning CP, all cases were immunopositive. CD25+ cells were expressed in focal or diffuse pattern either close or far from the epithelium. Diffuse distribution of positive cells throughout the connective tissue was seen in 60% of the cases and 32% showed focal or diffuse cellular pattern. Sixteen cases (64%) received score +++. It was identified that CD25+ cells are present in either a focal or a diffuse pattern in connective tissue. Significant differences in the density of cellular immunostaining between CG and CP were found. The greatest density was observed in CP cases, which suggests that the infiltrate of lymphocytes show a higher degree of cellular activation in periodontitis compared with gingivitis.

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Immunohistochemical analysis of S-100 protein-positive dendritic cells in human cutaneous squamous cell carcinomas, Bowen's diseases and basal cell carcinomas.

Journal of the Japan Society of the Reticuloendothelial System ◽

10.3960/jslrt1961.32.349 ◽

1992 ◽

Vol 32 (4) ◽

pp. 349-358

Author(s):

Yuiko Manabe ◽

Yuji Ohtsuki

Keyword(s):

Dendritic Cells ◽

Squamous Cell ◽

Basal Cell ◽

Immunohistochemical Analysis ◽

Squamous Cell Carcinomas ◽

Basal Cell Carcinomas ◽

S 100

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Ultrastructural study of langerhans cells in a murine UV-induced tumor

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100157358 ◽

1989 ◽

Vol 47 ◽

pp. 1074-1075

Author(s):

C.D. Bucana ◽

M.J. Song ◽

K. Dunner ◽

R. Sanchez ◽

M.L. Kripke

Keyword(s):

Dendritic Cells ◽

Tumor Cells ◽

Langerhans Cells ◽

Immunohistochemical Analysis ◽

Lymphoid Tissues ◽

Immunoperoxidase Technique ◽

Induced Tumors ◽

Infiltrating Cells ◽

S 100 ◽

Electron Lucent

Langerhans cells (LC) are dendritic cells of the macrophage-monocyte lineage that are found in the skin, other epithelial-1ined tissues and lymphoid tissues. LC are al so present in a wide variety of pathologic tissues. LC are characterized by the presence of a unique rod- or racquet-shaped pentalaminar organelle called the Langerhans cell granule. Ultrastructural and immunohistochemical analysis of several murine UV-induced tumors revealed the presence of infiltrating cells that are morphologically similar to LC in some tumors.Murine UV-induced tumors were processed for routine histology by paraffin embedding and conventional TEM. Samples were screened for morphology of tumor cells. Tumors in which LC were identified ultrastructurally were also identified by an immunoperoxidase technique for the presence of S-100 protein.Infiltrating cells that are positive for S-100 protein are found in some UV-induced tumors. These cells are not uniformly distributed in these tumors and their numbers varied significantly from region to region. They are found among tumor cells and their dendrites are observed between tumor cells. Ultrastructural analysis of these cells showed that they exhibit a more electron lucent cytoplasm than the surrounding cells in the tissue and they contain neither desmosomes nor tonofilaments.

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Detection of Vascular Dendritic Cells and Extracellular Calcium-Binding Protein S-100 in Foci of Calcification in Human Arteries.

ACTA HISTOCHEMICA ET CYTOCHEMICA ◽

10.1267/ahc.28.371 ◽

1995 ◽

Vol 28 (4) ◽

pp. 371-380 ◽

Cited By ~ 16

Author(s):

Yuri V. Bobryshev ◽

Reginald S. A. Lord

Keyword(s):

Dendritic Cells ◽

Calcium Binding ◽

Binding Protein ◽

Protein S ◽

Calcium Binding Protein ◽

Extracellular Calcium ◽

S 100 ◽

Human Arteries

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Immunohistochemical Characterization of Canine Transmissible Venereal Tumor

Veterinary Pathology ◽

10.1177/030098589603300301 ◽

1996 ◽

Vol 33 (3) ◽

pp. 257-263 ◽

Cited By ~ 64

Author(s):

E. Mozos ◽

A. Méndez ◽

J. C. Gómez-Villamandos ◽

J. Martín de las Mulas ◽

J. Pérez

Keyword(s):

Light Chain ◽

B Lymphocyte ◽

Protein S ◽

Lambda Light Chain ◽

Formalin Fixed Paraffin ◽

S 100 ◽

Formalin Fixed Paraffin Embedded ◽

Progressive Growth ◽

Staining Techniques ◽

Alpha 1 Antitrypsin

The collective immunohistochemical expression of human lysozyme, human alpha-1-antitrypsin, human CD3 antigen, calf vimentin, human keratins, human lambda light chains, canine immunoglobulins IgG, IgM, and bovine protein S-100 has been analyzed on formalin-fixed, paraffin-embedded tissue sections of 25 spontaneous canine transmissible venereal tumors (CTVT) from both genital and extragenital locations using the avidin-biotin-peroxidase complex technique. Lysozyme immunoreactivity was detected in 10/25 CTVT, alpha-1-antitrypsin in 14/25 CTVT, and vimentin in 25/25 CTVT. All CTVT cells were negative to keratins 5 + 8 of the Moll catalogue (RCK-102), S-100 protein, lambda light-chain immunoglobulins, IgG, IgM, and CD3 antigen. The intratumoral T- and B-lymphocyte infiltrate was differentiated using CD3 antigen, lambda light-chain immunoglobulins, IgG, and IgM, and this technique could be useful to evaluate the regressive or progressive growth stage of venereal tumors. Our findings support the hypothesis of a histiocytic immunophenotype for CTVT, and these staining techniques could be used in the differential diagnosis with lymphomas.

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Locally advanced malignant solitary fibrous tumour successfully treated with conversion chemotherapy, operation and postoperative radiotherapy: a case report

Journal of International Medical Research ◽

10.1177/0300060521996940 ◽

2021 ◽

Vol 49 (3) ◽

pp. 030006052199694

Author(s):

Zhi-Ke Li ◽

Jie Liu ◽

Chen Chen ◽

Ke-Yi Yang ◽

Yao-Tiao Deng ◽

...

Keyword(s):

Rna Binding ◽

Binding Protein ◽

Postoperative Radiotherapy ◽

Locally Advanced ◽

Rna Binding Protein ◽

Disease Free Survival ◽

Protein S ◽

Solitary Fibrous Tumour ◽

Final Pathology ◽

S 100

Preoperative diagnosis of solitary fibrous tumour (SFT) may not provide a complete tumour picture and may be inaccurate. There is no standard treatment for locally advanced or metastasised malignant SFT (MSFT). Here, the case of a 17-year-old male patient with final pathology diagnosis of MSFT is reported. Preoperative biopsy pathology results suggested an Ewing sarcoma that was positive for CD99 antigen, vimentin, friend leukaemia integration 1 transcription factor, apoptosis regulator Bcl-2, and synaptophysin; and negative for CD34 antigen, S-100 protein (S-100), smooth muscle antigen, cytokeratin, and Wilms tumour 1 associated protein. The Ki67 positive rate was 8%, so the patient initially received eight cycles of conversion chemotherapy (vincristine, etoposide, ifosfamide and pirarubicin for one cycle, and vincristine, doxorubicin, and cyclophosphamide/ifosfamide and etoposide for 7 cycles in total). The tumour shrunk significantly and was surgically removed. The final pathology diagnosis was MSFT that was positive for CD99 and signal transducer and activator of transcription 6, and negative for CD34, tumour protein 63, S-100, desmin, and epithelial membrane antigen. Fluorescence in situ hybridization showed no gene translocation in EWS RNA binding protein 1, SS18 subunit of BAF chromatin remodelling complex or FUS RNA binding protein. The patient finally accepted adjuvant radiotherapy of 5600 cGy. Disease-free survival has been > 1 year, with no recurrence or metastasis detected to date. MSFT is rare and treatment for locally advanced or metastatic MSFT remains controversial. The efficacy of the present therapeutic strategy requires further research.

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