Syndecan-1 (CD138) and Ki-67 expression in odontogenic cystic lesions

The aim of this study was to assess the immunohistochemical expression of syndecan-1 (CD138) and Ki-67 in radicular cysts (RC), dentigerous cysts (DC) and keratocystic odontogenic tumors (KOT). Thirty-five RC, 22 DC and 17 KOT were used in the study and immunohistochemical reactions using anti-syndecan-1 and anti-Ki-67 antibodies were performed by the streptavidin-biotin-peroxidase method. Fisher's exact test and Spearman's correlation coefficient were used for statistical analysis of data. Among the studied lesions, no differences in the syndecan-1 expression were observed, but the suprabasal expression of Ki-67 was significantly higher in KOT (p<0.0001), when compared with RC and DC. In RC, there was positive correlation between the expression (p=0.02) and intensity (p=0.0001) of syndecan-1 and between the intensity of syndecan-1 and Ki-67 expression (p=0.01). In the KOT, Ki-67 expression in the suprabasal layer correlated positively with the expression (p=0.01) and intensity (p=0.01) of syndecan-1. The expression of syndecan-1 does not seem to be a determinant factor of the distinct histopathological features and biological behavior of the studied lesions. Nevertheless, positive correlation between syndecan-1 and a cell proliferation marker was observed in RC and KOT.

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Comparative Study of the Minichromosome Maintenance Proteins Complex (MCM 4/5/6) in Ameloblastoma and Unicystic Ameloblastoma

International Journal of Surgical Pathology ◽

10.1177/1066896918778341 ◽

2018 ◽

Vol 26 (8) ◽

pp. 714-720

Author(s):

Delmira Apellániz ◽

Vanesa Pereira-Prado ◽

Estefania Sicco ◽

Gabriela Vigil-Bastitta ◽

Rogelio González-González ◽

...

Keyword(s):

Cellular Proliferation ◽

Label Index ◽

Biological Behavior ◽

Complex Materials ◽

Minichromosome Maintenance ◽

Ki 67 ◽

Unicystic Ameloblastoma ◽

Maxillary Region ◽

Cell Proliferation Marker ◽

Higher Sensitivity

Introduction. Solid/conventional ameloblastoma (AM) and unicystic ameloblastoma (UAM) are the most frequent benign epithelial odontogenic tumors located in the maxillary region, and their treatment usually consists of extensive surgical resection. Therefore, it is relevant to study molecular markers to better understand the biological behavior of these tumors. The aim of this study was to describe and compare the expression of proteins related to cellular proliferation: Ki-67 and MCM4-6 complex. Materials and Methods. An immunohistochemistry technique was performed, with antibodies against Ki-67, MCM4, MCM5, and MCM6, in 10 AM and 10 UAM tumors. The results were quantified using label index and analyzed statistically. Results. AM and UAM had greater expression of MCM6, followed by MCM5, MCM4, and Ki-67 ( P < .05). Immunoexpression of Ki-67 and MCM5 was exclusively nuclear, whereas the expression of MCM4 and MCM6 was nuclear and cytoplasmic. Conclusion. The results suggest that MCM5 is a trustable cell proliferation marker with higher sensitivity compared with Ki-67 and may be useful to predict the biological behavior of AM and UAM. Despite this, further studies are necessary, including a correlation with clinical parameters to confirm these findings.

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Association between expression of Ki-67, parafibromin, p27, and Rb protein and the biological behavior in parathyroid tumors

Endocrine Abstracts ◽

10.1530/endoabs.32.p536 ◽

2013 ◽

Cited By ~ 1

Author(s):

Keiko Ohkuwa ◽

Chie Masaki ◽

Junko Akaishi ◽

Akifumi Suzuki ◽

Takashi Uruno ◽

...

Keyword(s):

Biological Behavior ◽

Ki 67 ◽

Rb Protein ◽

Parathyroid Tumors

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Changes in Cellular Regulatory Factors before and after Decompression of Odontogenic Keratocysts

Journal of Clinical Medicine ◽

10.3390/jcm10010030 ◽

2020 ◽

Vol 10 (1) ◽

pp. 30

Author(s):

Slmaro Park ◽

Han-Sung Jung ◽

Young-Soo Jung ◽

Woong Nam ◽

Jung Yul Cha ◽

...

Keyword(s):

Recurrence Rate ◽

Biological Behavior ◽

Nuclear Antigen ◽

Epithelial Cyst ◽

Ki 67 ◽

Proliferation Markers ◽

Odontogenic Keratocysts ◽

Wide Range ◽

Before And After ◽

Cyst Lining

Decompression followed by enucleation, which is one of the treatments used for odontogenic keratocysts (OKCs), is frequently used in OKC lesions of large sizes. This method offers the advantage of minimizing the possibility of sensory impairment without creating a wide-range bone defect; moreover, the recurrence rate can be significantly lower than following simple enucleation. This study aimed to assess the changes in histology and expression of proliferation markers in OKCs before and after decompression treatment. A total of 38 OKC tissue samples from 19 patients who had undergone decompression therapy were examined morphologically and immunohistochemically to observe changes in proliferative activity before and after decompression. The markers used for immunohistochemistry (IHC) staining were Bcl-2, epidermal growth factor receptor (EGFR), Ki-67, P53, PCNA, and SMO. The immunohistochemistry positivity of the 6 markers was scored by using software ImageJ, version 1.49, by quantifying the intensity and internal density of IHC-stained epithelium. The values of Bcl-2, Ki-67, P53, proliferating cell nuclear antigen (PCNA), and SMO in OKCs before and after decompression showed no significant change. No correlation between clinical shrinkage and morphologic changes or expression of proliferation and growth markers could be found. There was no statistical evidence that decompression treatment reduces potentially aggressive behavior of OKC within the epithelial cyst lining itself. This might indicate that decompression does not change the biological behavior of the epithelial cyst lining or the recurrence rate.

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Correlation of 18F-FDG PET/CT SUVmax with clinical features, D-dimer and LDH in patients with primary intestinal lymphoma

Journal of International Medical Research ◽

10.1177/03000605211029809 ◽

2021 ◽

Vol 49 (7) ◽

pp. 030006052110298

Author(s):

Shuo Zhou ◽

Wenxin Chen ◽

Meifu Lin ◽

Guobao Chen ◽

Cailong Chen ◽

...

Keyword(s):

Fdg Pet ◽

Clinical Staging ◽

Imaging Features ◽

Intestinal Lymphoma ◽

Ki 67 ◽

Positive Correlation ◽

Pet Ct ◽

18F Fdg ◽

Fdg Pet Ct ◽

D Dimer

Objective To investigate the characteristics of fluorine-18-deoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) maximum standardized uptake value (SUVmax) in primary intestinal lymphoma (PIL) and its correlation with D-dimer and lactate dehydrogenase (LDH). Methods Fifty-two patients diagnosed with PIL from June 2016 to December 2019 were analyzed. All patients underwent 18F-FDG PET/CT. The relationships between SUVmax and different pathological subtypes, clinical stages and risk grades were analyzed. The correlations between SUVmax and Ki-67, LDH and D-dimer were determined. Additionally, PET/CT imaging results were collected from 35 patients with primary intestinal cancer (PIC) and compared with the imaging features of PIL. Results SUVmax was significantly different between PIL and PIC groups and various PIL pathological subgroups. Patients in the high-risk PIL group had markedly higher SUVmax values than the intermediate-risk and low-risk groups. A significant positive correlation was observed between SUVmax and Ki-67 in patients with PIL. SUVmax was significantly different between the elevated and normal D-dimer groups. D-dimer showed a positive correlation with SUVmax. Conclusion 18F-FDG PET/CT SUVmax reflects the aggressiveness of lymphoma to a certain degree, is correlated with Ki-67 and determines the risk grades of PIL. Moreover, it facilitates differential diagnosis, clinical staging and treatment based on D-dimer levels.

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Expression of 58-kD Microspherule Protein (MSP58) is Highly Correlated with PET Imaging of Tumor Malignancy and Cell Proliferation in Glioma Patients

Cellular Physiology and Biochemistry ◽

10.1159/000438656 ◽

2016 ◽

Vol 38 (2) ◽

pp. 635-645 ◽

Cited By ~ 2

Author(s):

Wei Lin ◽

Shu-hui Dai ◽

Tao Chen ◽

Nobuyuki Kawai ◽

Keisuke Miyake ◽

...

Keyword(s):

Cell Proliferation ◽

Significant Positive Correlation ◽

Tumor Grade ◽

Molecular Therapy ◽

Ki 67 ◽

Positive Correlation ◽

Flt Pet ◽

Pet Tracer ◽

Positron Emission ◽

Regulation Functions

Background/Aims: The nucleolar 58-kDa microspherule protein (MSP58) has important transcriptional regulation functions and plays a crucial role in the tumorigenesis and progression of cancers. 3'-deoxy-3'-[18F]fluorothymidine (FLT) has emerged as a promising positron emission tomography (PET) tracer for evaluating tumor malignancy and cell proliferation. Methods: In the present study, the expression of MSP58 was evaluated by immunohistochemistry and the corresponding PET image was examined using FLT-PET in 55 patients with various grades of gliomas. Results: The immunoreactivity score (IRS) of MSP58 increased with tumor grade with grade IV gliomas exhibiting the highest expression and showed a highly significant positive correlation with the Ki-67 index (r = 0.65, P < 0.001). The IRS of MSP58 in the tumor showed a highly significant positive correlation with corresponding FLT uptake value (r = 0.61, P < 0.001). The correlation between MSP58 expression and glioma malignancy was also confirmed by immunofluorescence, RT-PCR and western blot analysis. FLT uptake value also exhibited a highly significant positive correlation with the Ki-67 index (r = 0.59, P < 0.001). Kaplan-Meier analysis revealed that MSP58 expression has a significant prognostic ability for the overall survival time similar to that found in the uptake value of FLT-PET. Conclusion: These results indicate that MSP58 plays an important role in cell proliferation and will be one of the potential candidates of molecular therapy targeting proliferation. FLT-PET might be used as an early measure of treatment response in the proliferation-targeted therapy.

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Immunohistochemical expression of HER ‐ 2 and Ki ‐ 67 in granulosa cell tumor in bitches

Reproduction in Domestic Animals ◽

10.1111/rda.13903 ◽

2021 ◽

Author(s):

Ana Carina Heil da Silva Matos ◽

Angélica Consalter ◽

Bárbara Paula Santos Batista ◽

Ana Beatriz Monteiro Fonseca ◽

Ana Maria Reis Ferreira ◽

...

Keyword(s):

Granulosa Cell ◽

Granulosa Cell Tumor ◽

Cell Tumor ◽

Immunohistochemical Expression ◽

Ki 67 ◽

Her 2

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The Role of Ki-67 Immunoexpression in Predicting the Biological Behavior of Retinoblastoma Tumor in Tanzania

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqy090.090 ◽

2018 ◽

Vol 150 (suppl_1) ◽

pp. S37-S37

Author(s):

James Yahaya

Keyword(s):

Biological Behavior ◽

Ki 67 ◽

Retinoblastoma Tumor

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Analysis of the immunohistochemical expressions of p53, bcl-2 and Ki-67 in colorectal adenocarcinoma and their correlations with the prognostic factors

Arquivos de Gastroenterologia ◽

10.1590/s0004-28032010000200005 ◽

2010 ◽

Vol 47 (2) ◽

pp. 141-147 ◽

Cited By ~ 19

Author(s):

Hunaldo Lima de Menezes ◽

Mário Jorge Jucá ◽

Edmundo Guilherme de A. Gomes ◽

Benício L. Bulhões B. P. Nunes ◽

Henrique Oliveira Costa ◽

...

Keyword(s):

Prognostic Factors ◽

Cell Differentiation ◽

Colorectal Adenocarcinoma ◽

Sao Paulo ◽

Immunohistochemical Expression ◽

Ki 67 ◽

Disease Free Interval ◽

Free Interval ◽

Specific Mortality ◽

Disease Free

CONTEXT: Search of tumors markers that allow treatment with higher survival rates, and indicate the response to treatment and recurrence of cancer OBJECTIVE: To analyze the immunoexpression of the proteins p53, bcl-2 and Ki-67 in colorectal adenocarcinoma and correlate them with the clinical-pathological prognostic factors. METHOD: Tissue microarray paraffin blocks were made from colorectal adenocarcinoma tissue resected from 82 patients who had undergone surgery but not chemotherapy or radiotherapy, at "Hospital São Paulo", São Paulo, SP, Brazil, between 2002 and 2005. Thin sections (4 µm) were subjected to immunohistochemical reactions, and immunoexpression staining scores were obtained. The scores were correlated with the degree of cell differentiation, staging, disease-free interval, recurrence, survival and specific mortality. The study variables were analyzed using the chi-square and Kaplan-Meier tests to investigate associations with the markers. The significance of the differences between the curves of the disease-free interval and survival was analyzed using the Logrank and Wilcoxon tests. RESULTS: The immunohistochemical expression of p53 was positive in 70 tumors (85.4%) and negative in 12 (14.6%). The expression of bcl-2 was positive in 26 (31.7%) and negative in 56 (68.3%). The expression of Ki-67 was positive in 62 (75.6%) and negative in 20 (24.4%). There was no statistically significant correlation between the expressions of these markers separately or in conjunction, in relation to the degree of cell differentiation, staging, disease-free interval, survival and specific mortality. In relation to recurrence, there was a statistically significant correlation with positive expression of Ki-67 (P = 0.035). CONCLUSION: The immunohistochemical expression of Ki-67 in colorectal cancer is associated with recurrence of this disease.

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