virus screening
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2021 ◽  
Vol 8 ◽  
Author(s):  
Md Zulfekar Ali ◽  
Mohammad Moktader Moula ◽  
Zafar Ahmed Bhuiyan ◽  
Md Giasuddin ◽  
Hyun-Jin Shin

Chicken astrovirus (CAstV) and avian nephritis virus (ANV) are enteric viruses of poultry and have infected a wide range of poultry species worldwide, causing runting-stunting syndrome (RSS), which requires virus screening and results in serious economic damage. No confirmed cases have been reported from Bangladesh. In the present study, CAstV and ANV were monitored in Bangladesh. We monitored samples for CAstV and ANV and compared their genomic sequences to other reference strains. We found 8/31 flocks (25.8%) were positive for CAstV, 6/31 flocks (19.3%) had mixed infection of CAstV and ANV, and 1 flock (3.2%) was positive for ANV. Only ANV and a combination of CAstV and ANV were found in broilers and broiler breeders, but CAstV was found in all types of chickens. We isolated two of each from CAstV and ANV through specific pathogen-free chicken embryonated eggs via the yolk sac route. Phylogenetic analysis based on the ORF1b conserved region of CAstV and ANV suggested that the locally circulating strain was closely related to the strains isolated from India and Brazil. This report is the first molecular characterization of CAstV and ANV in Bangladesh. This study highlights that CAstV and ANV are circulating in Bangladeshi poultry.


2021 ◽  
Vol 5 (11) ◽  
pp. 1095-1098
Author(s):  
Funda PEPEDİL TANRİKULU ◽  
Didar YANARDAĞ AÇIK ◽  
Bilal AYGUN ◽  
Mehmet BANKİR ◽  
Mikail ÖZDEMİR

Hepatology ◽  
2021 ◽  
Author(s):  
Nathan S. Ramrakhiani ◽  
Vincent L. Chen ◽  
Michael Le ◽  
Yee Hui Yeo ◽  
Scott D. Barnett ◽  
...  

2021 ◽  
Author(s):  
lham ghazizadeh ◽  
Ali Neshastehriz ◽  
Ali Dehghani Firoozabadi ◽  
Esmail Saievar-Iranizad ◽  
Samira Einali

Abstract It has been necessary to use methods that can detect the specificity of a virus during virus screening. In this study, we use a dual platform to identify any spiked virus and specific SARS-CoV-2 antigen, sequentially. We introduce a natural bed-receptor surface as Microparticle Vesicle-Galactins1 (MV-gal1) with the ability of glycan binding to screen every spiked virus. MV are the native vesicles which may have the gal-1receptor. Gal-1 is the one of lectin receptor which can bind to glycan. After dropping the MV-gal1 on the SCPE/GNP, the sensor is turned on due to the increased electrochemical exchange with [Fe(CN)6] -3/-4 probe. Dropping the viral particles of SARS-CoV-2 cause to turn off the sensor with covering the sugar bond (early screening). Then, with the addition of Au /Antibody-SARS-CoV-2 on the MV-gal1@ SARS-CoV-2 Antigen, the sensor is turned on again due to the electrochemical amplifier of AuNP (specific detection). As a result, our sensor has the capacity of screening of every spike virus, and the specific detection of covid-19 (LOD: 4.57 × 102 copies/mL) by using the natural bed-receptor and a specific antibody in the solid biosensor, for the first time.


mSphere ◽  
2021 ◽  
Author(s):  
Evangelia Georgia Kostaki ◽  
Georgios A. Pavlopoulos ◽  
Kleio-Maria Verrou ◽  
Giannis Ampatziadis-Michailidis ◽  
Vaggelis Harokopos ◽  
...  

Our study based on current state-of-the-art molecular epidemiology methods suggests that virus screening and public health measures after the lifting of travel restrictions prevented SARS-CoV-2 onward transmission from imported cases during summer 2020 in Greece. These findings provide important data on the efficacy of targeted public health measures and have important implications regarding the safety of international travel during a pandemic.


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