scholarly journals Histochemistry, content and chemical composition of essential oil in different organs of Alpinia zerumbet

2013 ◽  
Vol 43 (10) ◽  
pp. 1811-1816 ◽  
Author(s):  
Caroline Nery Jezler ◽  
Ricardo Silva Batista ◽  
Péricles Barreto Alves ◽  
Delmira da Costa Silva ◽  
Larissa Corrêa do Bomfim Costa
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Oil Content ◽  
Folk Medicine ◽  
Major Constituent ◽  
Secretory Structures ◽  
Asian Origin ◽  
Histochemical Test ◽  
Alpinia Zerumbet

Alpinia zerumbet is a medicinal plant from Asian origin used in folk medicine for the treatment of hypertension, which effect is attributed to terpinen-4-ol, the major component of the essential oil. The objective of this work was to identify the essential oil secretory structures in the leaf, flower, root and rhizome of this plant, and analyze the content and the chemical composition of the oil in the different organs of the plant. Sections were subjected to histochemical test with Nadi reagent for in situ localization of secretion. The essential oil extraction was performed by hydrodistillation in a Clevenger apparatus and the compounds were identified in CG-EM/FID. The histochemical test was positive for terpenoids, confirming the presence of essential oil stored in secretory structures named oils cells present in all analyzed organs. The higher essential oil content was found on the leaf (0.30%), while the petal and the rhizome presented content of 0.10% and 0.06%, respectively. It was not possible to determine essential oil content of the root due to the low amount of biomass produced. There were qualitative and quantitative differences in the chemical composition of the essential oil in the different plant organs, but the major constituent in all of them was the terpinen-4-ol, followed by 1,8 cineol in the leaf and by the α-terpineol in the flower and rhizome.

scholarly journals Harvest time and plant age on the content and chemical composition of the essential oil of Alpinia zerumbet

2012 ◽  
Vol 30 (3) ◽  
pp. 385-390 ◽  
Author(s):  
Martiely S Santos ◽  
Caroline N Jezler ◽  
Ariana RMF de Oliveira ◽  
Rosilene A Oliveira ◽  
Marcelo S Mielke ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Secondary Metabolism ◽  
Oil Content ◽  
Plant Age ◽  
Harvest Time ◽  
Biosynthetic Activity ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Alpinia Zerumbet

Alpinia zerumbet is an aromatic and medicinal plant rich in essential oil, known as colônia. Essential oils are derived from secondary metabolism and may be a source of raw materials for cosmetic, pharmaceutical, food and perfumery industry. The plant secondary metabolism and biosynthetic activity can vary according to endogenous and exogenous factors to which it is exposed. In this context, in this study we evaluated the influence of harvest time and plant age of Alpinia zerumbet on biomass and essential oil production. For the harvest time experiment the plants of A. zerumbet were harvested at different times (8, 10, 12, 14, 16 and 18 h), using a completely randomized design with four replications. In the plant age experiment the seedlings were propagated by division of rhizomes and grown in a completely randomized design with treatments consisting of four ages (3, 6, 9 and 12 months after transplanting), with seven replications. The extractions of the essential oil were performed by oil hydrodistillation in Clevenger apparatus and chemical analysis by gas chromatography coupled with mass spectrometry (CG-MS). There was an effect of harvest time on the essential oil content with the highest value (0.48%) found at 14:33 h with no change in the chemical composition. In relation to plant age, there was a significant increase in aboveground biomass of plants, accompanied by increases in height, number of shoots, and essential oil content and yield. The major compound terpinen-4-ol was present in higher concentrations in plants harvested between six and nine months old.

scholarly journals Chemical Composition and Antioxidant Activities of the Essential Oil from Tornabenea bischoffii (Apiaceae)

2011 ◽  
Vol 6 (8) ◽  
pp. 1934578X1100600
Author(s):  
Risoleta Ortet ◽  
Erik L. Regalado ◽  
Olivier P. Thomas ◽  
Jorge A. Pino ◽  
Miguel D. Fernández
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
In Vitro Assay ◽  
Cape Verde ◽  
Major Constituent ◽  
Vitro Assay

The chemical composition of Tornabenea bischoffii J. A. Schmidt essential oil from Cape Verde was studied by GC and GC/MS. Sixty volatile compounds were identified of which myristicin was the major constituent (33.6%). Using the 2,2-diphenyl-2-picrylhydrazyl free-radical scavenging method and the in vitro assay for prevention of lipid peroxidation by thiobarbituric reactive species, significant antioxidant activities were evidenced.

Essential oil content and chemical composition of Cymbopogon citratus inoculated with arbuscular mycorrhizal fungi under different levels of lead

2015 ◽  
Vol 76 ◽  
pp. 734-738 ◽  
Author(s):  
Caroline Lermen ◽  
Fabrício Morelli ◽  
Zilda Cristiani Gazim ◽  
Adriana Pereira da Silva ◽  
José Eduardo Gonçalves ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Oil Content ◽  
Arbuscular Mycorrhizal ◽  
Cymbopogon Citratus ◽  
Different Levels

scholarly journals Chemical composition of essential oil extracted from leaves of Campomanesia adamantium subjected to different hydrodistillation times

2017 ◽  
Vol 47 (1) ◽  
Author(s):  
Juliana Dantas de Oliveira ◽  
Daniely Karen Matias Alves ◽  
Mayker Lazaro Dantas Miranda ◽  
José Milton Alves ◽  
Marcelo Nogueira Xavier ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Oil Content ◽  
Chemical Constituents ◽  
Caryophyllene Oxide ◽  
Fruit Species ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACT: Campomanesia adamantium is a native fruit species of the Cerrado and is used in food and medicines and as bee pasture. The chemical composition of essential oils obtained from plants of the same species have varying constituent proportions due to the influence of extractive factors, environmental, genetic and ontogenetic. This study aimed to identify the influence of hydrodistillation time on the content and chemical composition of essential oil extracted from the leaves of C. adamantium . Treatments consisted of five extraction times (1, 2, 3, 4, and 5h) using Clevenger with five replications in a completely randomized design. It was observed that after two hours of hydrodistillation, the essential oil content remains constant. Regarding the chemical constituents of essential oil, variation of the proportions of the compounds tested occurred at all hydrodistillation times. The compounds spathulenol oxygenated sesquiterpenes and caryophyllene oxide were the majority in the five hydrodistillation times.

scholarly journals First Report on Yield and Chemical Composition of Essential Oil Extracted from Myrcia eximia DC (Myrtaceae) from the Brazilian Amazon

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 783
Author(s):  
Oberdan Oliveira Ferreira ◽  
Jorddy Neves da Cruz ◽  
Celeste de Jesus Pereira Franco ◽  
Sebastião Gomes Silva ◽  
Wanessa Almeida da Costa ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Steam Distillation ◽  
Brazilian Amazon ◽  
Major Constituent ◽  
Chemical Compositions ◽  
Mass Yield ◽  
New Information ◽  
The City

The essential oil (EO) of plants of the Myrtaceae family has diverse chemical composition and several applications. However, data on the oil yield, its composition, and its complete chemistry are still unavailable for some species belonging to this family, such as Myrcia eximia DC. In this study, the chemical compositions of the EOs of Myrcia eximia were evaluated by using gas chromatography (GC) alone and gas chromatography coupled with mass spectrometry (GC–MS). Samples for both evaluations were collected from the city of Magalhães Barata, State of Pará, Brazil, in 2017 and 2018. For the plant material collected in 2017, EO was obtained by hydrodistillation (HD) only, while, for the material collected in 2018, EO was obtained by hydrodistillation and steam distillation (SD), in order to evaluate the differences in chemical composition and mass yield of the EO. The yields of (E)-caryophyllene were 15.71% and 20.0% for the samples collected by HD in 2017 and 2018, respectively, while the yield was 15.0% for the sample collected by SD in 2018. Hexanal was found to be the major constituent in the EO obtained by HD, with yield of up to 26.09%. The oil yields reached 0.08% by using SD, and 0.01% and 0.36% for the samples collected in 2017 and 2018, respectively, using HD. The results of this study provide new information about the mass yield and chemical composition of Myrcia eximia DC, and they can add value and income to traditional populations, as well as facilitate the preservation of this species.

scholarly journals Proapoptotic Activity of Achillea membranacea Essential Oil and Its Major Constituent 1,8-Cineole against A2780 Ovarian Cancer Cells

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1582 ◽  
Author(s):  
Ashraf N. Abdalla ◽  
Usama Shaheen ◽  
Qasem M. A. Abdallah ◽  
Guido Flamini ◽  
Majdi M. Bkhaitan ◽  
...  
Keyword(s):  
Essential Oil ◽  
Folk Medicine ◽  
Fibroblast Cell ◽  
Normal Fibroblast ◽  
Major Constituent ◽  
Ovarian Cancer Cells ◽  
Ht29 Cells ◽  
Total Composition ◽  
Induced Apoptosis ◽  
Dose Dependent Increase

Among the hundreds of reported Achillea species, A. membranacea (Labill.) DC. is one of the six that grow in Jordan. Many species of this genus are used in folk medicine to treat a variety of ailments and several biological and pharmacological activities have been ascribed to their essential oil (EO). For this study, the EO obtained from a specimen of A. membranacea grown in Jordan was analyzed by GC-MS. Ninety-six compounds were detected, of which oxygenated monoterpenes was the predominant class (47.9%), followed by non-terpene derivatives (27.9%), while sesquiterpenes represented 14.2% of the total composition. The most abundant compound in the EO was 1,8-cineole (21.7%). The cytotoxic activity of the EO was evaluated against three cancer cell lines (MCF7, A2780 and HT29), and one normal fibroblast cell line (MRC5) by MTT assay. Significant growth inhibition was observed in EO-exposed A2780 and HT29 cells (IC50 = 12.99 and 14.02 μg/mL, respectively), while MCF7 and MRC5 were less susceptible. The EO induced apoptosis and increased the preG1 events in A2780 cells. 1,8-Cineole, the major constituent of the EO, exhibited submicromolar cytotoxicity against A2780 cells, and was 42 times more selective against MRC5 cells. Its cytotoxicity against A2780 cells was comparable with that of doxorubicin, but 1,8-cineole was more selective for MRC5 normal cells. Interestingly, 1,8-cineole enhanced apoptosis in A2780, and caused a remarkable dose-dependent increase in preG1 events. Thus, 1,8-cineole has demonstrated promising cytotoxic and proapoptotic properties.

scholarly journals Light intensities alter growth and essential oil of patchouli under shade nets

2022 ◽  
Vol 52 (5) ◽  
Author(s):  
Aurislaine Santos Ribeiro ◽  
Suzan Kelly Vilela Bertolucci ◽  
Alexandre Alves de Carvalho ◽  
Wesley Naves Tostes ◽  
Adriane Duarte Coelho ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Oil Content ◽  
Oil Yield ◽  
Light Intensities ◽  
Pogostemon Cablin ◽  
Aromatic Species ◽  
Pharmaceutical Industries ◽  
Essential Oil Yield

ABSTRACT: Pogostemon cablin (Blanco) Benth. is an aromatic species popularly known as patchouli. The essential oil rich in patchoulol extracted from leaves is used by the pharmaceutical industries. The objective was to investigate the effects of shade nets and shading intensities on P. cablin growth, anatomy, chemical composition and essential oil content. The experiment was conducted with two types of shade nets (black and Aluminet), three light intensities (30, 50, and 70% shading) and full sun. The different light intensities influenced the growth and the essential oil yield, and chemical composition. Patchouli plants grown in an environment with shading showed higher values for the leaf, stem, root ant total dry weights compared to full sun. At 50% of shading, Aluminet provided gain in stem and total dry weights. Aluminet and black net at 50 and 70% of shading enhance essential oil yield. Patchoulol and pogostol contents were higher under full sun, and, black net and Aluminet at 50% shading. The environment for patchouli cultivation can be improved by use shade nets, especially with Aluminet at 50% of shading.

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