Anti-Trypanosoma cruzi antibody detection in blood donors in the Southern Brazil

Introduction: Chagas’ disease is the leading cause of infectious myocarditis worldwide. This infection caused by Trypanosoma cruzi is usually life-long and asymptomatic; however, the third part of infected people can develop severe or even fatal cardiomyopathy. As the parasitemia in the chronic phase is both low-grade and intermittent, T. cruzi infection is principally detected by serology, although this method has sensitivity and specificity limitations.Objective: To determine the level of agreement between serologic and molecular tests in 658 voluntary blood donors from six provinces in the Colombian department of Santander.Materials and methods: We evaluated an array of diagnostic technologies by cross-section sampling performing a serological double diagnostic test for T. cruzi antibody detection (Chagas III ELISA™, BiosChile Group, and ARCHITECT Chagas CMIA™, Abbott), and DNA detection by polymerase chain reaction (PCR). We collected the demographic, clinical, and epidemiological information of participants. The sample size was calculated using Epidat™ and the statistical analysis was done with Stata 12.1™.Results: PCR was six times more sensitive in detecting T. cruzi infection than ELISA/CMIA with prevalence values of 1.8% (12/658) and 0.3% (2/658), respectively, and kappa=0.28 (95%CI: -0.03 - 0.59). In contrast, serology showed a sensitivity of 16.7% (95%CI: 2.09 - 48.4) and a specificity of 100% (95%CI: 99.4 - 100). All seropositive samples were found to be positive by PCR.Conclusions: The implementation of PCR as a complementary method for screening donors could reduce the probability of false negative and the consequent risk of transfusional-transmission of Chagas’ disease, especially in endemic regions.

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Faculty Opinions recommendation of Trypanosoma cruzi parasitemia in US blood donors with serologic evidence of infection.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1115479.571508 ◽

2008 ◽

Author(s):

Carlos Morel

Keyword(s):

Trypanosoma Cruzi ◽

Blood Donors ◽

Serologic Evidence

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Investigation regarding the presence of Trypanosoma cruzi in triatomines and humans in rural households in the State of Paraná, southern Brazil

Research Society and Development ◽

10.33448/rsd-v10i10.18366 ◽

2021 ◽

Vol 10 (10) ◽

pp. e54101018366

Author(s):

Ana Paula de Abreu ◽

Hevillyn Fernanda Lucas da Silva ◽

Marcella Paula Mansano Sarto ◽

Giullia Ferreira Iunklaus ◽

João Vitor Trovo ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Rural Households ◽

Etiologic Agent ◽

The State ◽

Molecular Techniques ◽

Triatoma Infestans ◽

Southern Brazil ◽

The North ◽

Intestinal Contents ◽

Cruzi Infection

Trypanosoma cruzi, the etiologic agent of Chagas disease (CD), is transmitted by hematophagous insects belonging to the subfamily Triatominae. After elimination of Triatoma infestans, the infestation of human dwellings by secondary species of vectors continues to pose a risk of transmission of the parasite. Our aim was to investigate the T. cruzi presence in triatomines and humans in rural households in the State of Paraná, southern Brazil. The capture of the insects was carried out by technicians of the municipalities after residents reported the outbreak. Five residents and 27 triatomines captured in four municipalities in the North and Midwest of the state were evaluated. The research of T. cruzi was carried out using parasitological, serological, and molecular techniques, in human blood, excreta, intestinal contents and insect macerate. Panstrongylus megistus, P. geniculatus and Triatoma sp. were identified. Ten specimens of P. megistus were captured in a house in Mandaguari with five residents and presented an infection rate of 70% for T. cruzi like. All residents tested negative for T. cruzi infection. Another 15 P. megistus were captured in the peridomicile in Janiópolis and had 100.0% positivity. The only adult specimen of P. geniculatus captured in the intradomicile in Amaporã, as well as the nymph of Triatoma in the peridomicile in Paiçandu, were negative. The finding of P. megistus naturally infected by T. cruzi in households in rural area of Paraná demonstrates a potential risk of vector transmission of CD in these regions.

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Frequency of RHD variants in Brazilian blood donors from Parana State, Southern Brazil

Transfusion and Apheresis Science ◽

10.1016/j.transci.2016.04.016 ◽

2016 ◽

Vol 55 (1) ◽

pp. 120-124

Author(s):

Joana Maira Valentini Zacarias ◽

Elizangela Mendes de Figueiredo Pereira ◽

Jeane Eliete Laguila Visentainer ◽

Gláucia Andréia Soares Guelsin ◽

Fabiano Cavalcante de Melo ◽

...

Keyword(s):

Blood Donors ◽

Southern Brazil

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Seroprevalence Study of Anti-HEV IgG among Different Adult Populations in Corsica, France, 2019

Microorganisms ◽

10.3390/microorganisms7100460 ◽

2019 ◽

Vol 7 (10) ◽

pp. 460 ◽

Cited By ~ 1

Author(s):

Capai ◽

Masse ◽

Gallian ◽

Souty ◽

Isnard ◽

...

Keyword(s):

Risk Factors ◽

General Practice ◽

Blood Donors ◽

Adult Population ◽

Strong Association ◽

Hepatitis E ◽

Cross Sectional Study ◽

Antibody Detection ◽

P Value ◽

Cross Sectional

Hepatitis E virus (HEV) is a major cause of acute hepatitis worldwide. In France, hyperendemic areas including Corsica have an anti-HEV Immunoglobulin G (IgG) prevalence higher than 50%. The aim of this study was to determine the seroprevalence of anti-HEV IgG in three adult populations in Corsica and the risk factors associated with antibody detection. Between 2017 and 2019, a total of 930 individuals, including 467 blood donors, 393 students or university staff members and 70 patients from general practice, were tested for the presence of anti-HEV IgG using the Wantai HEV IgG enzyme immunoassay kit and filled a questionnaire. The association between seropositivity and potential risk factors was tested with univariate and multivariate analyses. Out of the 930 samples, 52.3% (486/930) were seropositive—54.4% (254/467) among blood donors, 47.6% (187/393) among university students and 64.3% (45/70) among patients of general practice. Three main risk factors were identified: (i) skinning and butchering (Adjusted Odds Ratio aOR = 2.76, 95% confidence interval [95% CI] [1.51–5.37]; p-value < 10−3), (ii) consumption of a local pork live raw sausage (fittonu) (aOR = 1.95 95% CI [1.45–2.64]; p-value = 10−5), and (iii) increasing age (p-value = 0.003). Seropositivity rates between the different populations were homogeneous after age stratification. This cross-sectional study indicates a high anti-HEV IgG seroprevalence in the Corsican adult population, not significantly different between women and men and increasing with age. This serosurvey also showed homogeneity regarding the exposure to HEV among three different types of populations. Finally, we confirmed the endemicity of Corsica with respect to HEV and identified a strong association between consumption of figatellu/fittonu and the practice of skinning and butchering with the detection of anti-HEV IgG.

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