scholarly journals Influence of some sugars on xylanase production by Aspergillus awamori in solid state fermentation

2002 ◽  
Vol 45 (4) ◽  
pp. 431-437 ◽  
Author(s):  
Judith Liliana Solórzano Lemos ◽  
Nei Pereira Junior
Keyword(s):  
Solid State ◽  
Carbon Source ◽  
Sugar Cane ◽  
Sugar Cane Bagasse ◽  
Aspergillus Awamori ◽  
Birchwood Xylan ◽  
Xylanase Production ◽  
Low Levels ◽  
Xylosidase Activity

Aspergillus awamori showed high extracellular endoxylanase (100 U/ml) and beta-xylosidase activities (3.5 U/ml) when grown on milled sugar cane bagasse as the principal carbon source without treatment. Partial characterization of xylanases showed that the apparent values of Km were 3.12 ± 0.05 mg/ml for endoxylanase (in birchwood xylan) and 0.45 ± 0.05 mM for beta-xylosidase (in p -nitrophenyl beta-D-xylanopiranoside). Corresponding values of Vmax were 6.63 ± 0.02 and 0.078 ± 0.02 mumol/min. Gradual repression of endoxylanase activity was observed when increasing concentrations of glucose and xylose (1, 2, 4, 6 and 8 g of carbohydrate / 4 g of sugar cane bagasse) were added to production media. In contrast, beta-xylosidase activity was stimulated using low levels of carbohydrates (1 g xylose or glucose/ 4 g of sugar cane bagasse).

Production and Characterization of Amylases by Aspergillus niger under Solid State Fermentation Using Agro Industrials Products

2006 ◽  
Vol 2 (3) ◽  
Author(s):  
Michele Rigon Spier ◽  
Adenise Lorenci Woiciechowski ◽  
Luciana Porto de Souza Vandenberghe ◽  
Carlos Ricardo Soccol
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Sugar Cane ◽  
Maximum Yield ◽  
Initial Moisture Content ◽  
Cassava Starch ◽  
Sugar Cane Bagasse ◽  
Production Costs

The use of agro industrials products cassava starch and sugar cane bagasse present a great potential as substrate and support, respectively, showing low production costs for amylases production under solid-state fermentation by Aspergillus niger LPB 28. The effect of various factors was examined and some variables were optimized to ?-amylase and amyloglucosidase production. Inoculum rate in different concentrations (105, 106, 107 and 108 spores/g of cassava starch) were used to inoculate the solid substrate for the SSF. The material was supplemented with nitrogen sources; calcium source and micronutrients solution. The ratios of cassava starch to sugar cane bagasse (1/1; 1.5/1; 1/1.5; 1/2; 2/1; 1/3; 3/1) were tested and the initial moisture content varying 80, 85 and 90%. Cultivation was carried out at temperatures 25, 30 and 35ºC for 48, 60 and 72 h. Study of influence of pH initial in SSF was conduced in pH 4.0, 5.0, 6.0, 7.0 and 8.0. Statistical analysis was carried out using the software STATISTICA 5.1 (StatSoft, Tulsa, OK, USA). A 32 complete factorial design (2 factors, 3 levels and 9 experiments) was used to study the effect of the factors in ?-amylase and amyloglucosidase production. The results showed cassava starch and sugar cane bagasse at the ratios of 2/1 was the best for optimum production of ?-amylase and amyloglucosidase. The maximum yield (?-amylase 1732.95 U/g of cassava starch and amyloglucosidase 2044.94 U/g of cassava starch) was achieved with optimized process parameters such as incubation period (60 hours), moisture level was 90%, inoculation rate (105 spores/g of dried material), pH 4.0 and fermentation temperature (30ºC). The nitrogen source (KNO3) also enhanced the ?-amylase and amyloglucosidase activities. In kinetic characterization of enzymes the Michaelis-Menten constant KM and maximum velocity Vmax (KM 10.84 g/l, Vmax 3.42 g/l) for ?-amylase and KM 11.32 g/l and Vmax 5.18 g/l for amyloglucosidase.

scholarly journals Morphogenesis and Production of Enzymes byPenicillium echinulatumin Response to Different Carbon Sources

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Laísa dos Reis ◽  
Marli Camassola ◽  
Aldo José Pinheiro Dillon
Keyword(s):  
Enzymatic Activity ◽  
Sugar Cane ◽  
Filter Paper ◽  
Carbon Sources ◽  
Sugar Cane Bagasse ◽  
Xylanase Production ◽  
Penicillium Echinulatum ◽  
The Media ◽  
Filter Paper Activity ◽  
Second Generation Ethanol

The effect of different carbon sources on morphology and cellulase and xylanase production ofPenicillium echinulatumwas evaluated in this work. Among the six carbon sources studied, cellulose and sugar cane bagasse were the most suitable for the production of filter paper activity, endoglucanases, xylanases, andβ-glucosidases. However, sucrose and glucose showedβ-glucosidase activities similar to those obtained with the insoluble sources. The polyacrylamide gels proved the enzymatic activity, since different standards bands were detected in the media mentioned above. Regarding morphology, it was observed that the mycelium in a dispersed form provided the greatest enzymatic activity, possibly due to greater interaction between the substrate and hyphae. These data are important in understanding the physiology of fungi and could contribute to obtaining enzyme with potential application in the technology of second generation ethanol.

scholarly journals Xylanase production by Aspergillus awamori under solid state fermentation conditions on tomato pomace

2011 ◽  
Vol 42 (4) ◽  
pp. 1585-1597 ◽  
Author(s):  
Marcelo A. Umsza-Guez ◽  
Ana B. Díaz ◽  
Ignacio de Ory ◽  
Ana Blandino ◽  
Eleni Gomes ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Aspergillus Awamori ◽  
Xylanase Production ◽  
Fermentation Conditions ◽  
Tomato Pomace

Lipase production by solid state fermentation of olive cake and sugar cane bagasse

1998 ◽  
Vol 5 (1-4) ◽  
pp. 75-78 ◽  
Author(s):  
J Cordova ◽  
M Nemmaoui ◽  
M Ismaı̈li-Alaoui ◽  
A Morin ◽  
S Roussos ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Sugar Cane ◽  
Lipase Production ◽  
Sugar Cane Bagasse ◽  
Olive Cake

scholarly journals Characterization of an Exoinulinase Produced by Aspergillus terreus CCT 4083 Grown on Sugar Cane Bagasse

2010 ◽  
Vol 58 (14) ◽  
pp. 8386-8391 ◽  
Author(s):  
Juliana B. Coitinho ◽  
Valéria M. Guimarães ◽  
Maíra N. de Almeida ◽  
Daniel L. Falkoski ◽  
José H. de Queiróz ◽  
...  
Keyword(s):  
Sugar Cane ◽  
Aspergillus Terreus ◽  
Sugar Cane Bagasse

scholarly journals Characterization of Sugar Cane Bagasse Ash: A Comparison to Densified Silica Fume and Fly Ash

2021 ◽  
Vol 9 (3) ◽  
pp. 88-93
Author(s):  
Asma A. Hussein
Keyword(s):  
Chemical Composition ◽  
Sugar Cane ◽  
Pozzolanic Reaction ◽  
Sugar Cane Bagasse ◽  
Hardened Concrete ◽  
Pozzolanic Material ◽  
Concrete Properties ◽  
Negative Impacts ◽  
Sugar Cane Bagasse Ash

In this study the Sugar cane bagasse ash (SCBA) was characterized for chemical composition, morphological and mineralogical properties. From XRF analysis it was found that the SCBA has SiO2, Al2O3, Fe2O3 and CaO content of 67.25%, 6.37%, 4.21% and 13% respectively and it has a very low LOI value of 0.34% and can be classified as Class F ASTM pozzolan according to ASTM C 618-2009. SCBA can be used as a pozzolanic material after grinding to less than 45 Micron. Based on the properties obtained from the characterization in this study it can be concluded that, utilization of SCBA in concrete will positively affect hardened concrete properties due to pozzolanic reaction, cementing properties and filling effect of SCBA. Moreover it will minimize the negative impacts associated with SCBA disposal.  

scholarly journals Characterization of a wollastonite glass-ceramic material prepared using sugar cane bagasse ash (SCBA) as one of the raw materials

2014 ◽  
Vol 98 ◽  
pp. 209-214 ◽  
Author(s):  
Silvio R. Teixeira ◽  
Agda E. Souza ◽  
Claudio L. Carvalho ◽  
Victor C.S. Reynoso ◽  
Maximina Romero ◽  
...  
Keyword(s):  
Ceramic Material ◽  
Sugar Cane ◽  
Glass Ceramic ◽  
Raw Materials ◽  
Sugar Cane Bagasse ◽  
Glass Ceramic Material ◽  
Sugar Cane Bagasse Ash
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