scholarly journals Microbial succession within an anaerobic sequencing batch biofilm reactor (ASBBR) treating cane vinasse at 55ºC

2009 ◽  
Vol 52 (4) ◽  
pp. 1027-1036 ◽  
Author(s):  
Maria Magdalena Ferreira Ribas ◽  
Fabio Alexandre Chinalia ◽  
Eloisa Pozzi ◽  
Eugenio Foresti
Keyword(s):  
Polyurethane Foam ◽  
Granular Sludge ◽  
Morphological Diversity ◽  
Biofilm Reactor ◽  
Organic Load ◽  
Specific Rate ◽  
Microbial Succession ◽  
Sequencing Batch Biofilm Reactor ◽  
Biomass Characterization ◽  
Anaerobic Biomass

The aim of this work was to investigate the anaerobic biomass formation capable of treating vinasse from the production of sugar cane alcohol, which was evolved within an anaerobic sequencing batch biofilm reactor (ASBBR) as immobilized biomass on cubes of polyurethane foam at the temperature of 55ºC. The reactor was inoculated with mesophilic granular sludge originally treating poultry slaughterhouse wastewater. The evolution of the biofilm in the polyurethane foam matrices was assessed during seven experimental phases which were thus characterized by the changes in the organic matter concentrations as COD (1.0 to 20.0 g/L). Biomass characterization proceeded with the examination of sludge samples under optical and scanning electron microscopy. The reactor showed high microbial morphological diversity along the trial. The predominance of Methanosaeta-like cells was observed up to the organic load of 2.5 gCOD/L.d. On the other hand, Methanosarcinalike microorganisms were the predominant archaeal population within the foam matrices at high organic loading ratios above 3.3 gCOD/L.d. This was suggested to be associated to a higher specific rate of acetate consumption by the later organisms.

Preliminary Biomass Characterization in a Sequencing Batch Biofilm Reactor

2004 ◽  
Vol 94 (12) ◽  
pp. 889-898 ◽  
Author(s):  
Claudio Di Iaconi ◽  
Roberto Ramadori ◽  
Antonio Lopez ◽  
Roberto Passino
Keyword(s):  
Biofilm Reactor ◽  
Sequencing Batch Biofilm Reactor ◽  
Biomass Characterization

Impact of carbon to nitrogen ratio on nitrogen removal at a low oxygen concentration in a sequencing batch biofilm reactor

2013 ◽  
Vol 67 (3) ◽  
pp. 612-618 ◽  
Author(s):  
Chong Tan ◽  
Fang Ma ◽  
Shan Qiu
Keyword(s):  
Dissolved Oxygen ◽  
Real Time ◽  
Removal Efficiency ◽  
Quantitative Polymerase Chain Reaction ◽  
Microbial Community Composition ◽  
Biofilm Reactor ◽  
Organic Load ◽  
Gene Copy ◽  
Ammonium Removal ◽  
Sequencing Batch Biofilm Reactor

A sequencing batch biofilm reactor (SBBR) filled with polyurethane (PU) was operated in low dissolved oxygen (DO) (0.1–0.9 mg/L) at three different carbon to nitrogen ratios (C/N ratios) (C/N = 1.8:1, 5.0:1 and 10.5:1) with focus on reactor performance and microbial community composition of nitrifying and denitrifying bacteria. Meanwhile, the ammonium, nitrite, nitrate, pH and dissolved oxygen were analyzed to monitor the process of nitrification-denitrification. The reactor had a steady ammonium removal in spite of a large variation in the initial ammonium loading (the ammonium removal efficiency was 87.2% at C/N 1.8:1, 92.9% at C/N 5.0:1, 88.4% at C/N 10.5:1). However, the total nitrogen (TN) removal was strongly affected by the initial organic loading (the TN removal efficiency was 55.2% at C/N 1.8:1, 74.3% at C/N 5.0:1, 79.0% at C/N 10.5:1). It was indicated that higher organic load promoted denitrification. The real-time quantitative polymerase chain reaction (real-time qPCR) analysis shown that Beta-proteobacteria occupied over 35% numerically. As for the Nitrosomonas sp., Nitrosospira sp. and Nitrospira sp. gene copy numbers, their abundance was generally in the order of magnitude of 1010. The Nitrosospira sp. fluctuated significantly in the range of 3.1–9.8% at different C/N ratios, while the Nitrosomonas sp. just changed a little.

Anaerobic Thermophilic (55°C) Treatment of TMP Whitewater in Reactors Based on Biomass Attachment and Entrapment

1999 ◽  
Vol 40 (11-12) ◽  
pp. 67-75 ◽  
Author(s):  
Sigrun J. Jahren ◽  
Jukka A. Rintala ◽  
Hallvard Ødegaard
Keyword(s):  
Granular Sludge ◽  
Biofilm Reactor ◽  
Upflow Anaerobic Sludge Blanket ◽  
Hybrid Reactor ◽  
Anaerobic Sludge ◽  
Loading Rates ◽  
Anaerobic Reactors ◽  
Multi Stage ◽  
Degradation Rates ◽  
Thermomechanical Pulping

Thermomechanical pulping (TMP) whitewater was treated in thermophilic (55°C) anaerobic laboratory-scale reactors using three different reactor configurations. In all reactors up to 70% COD removals were achieved. The anaerobic hybrid reactor, composed of an upflow anaerobic sludge blanket (UASB) and a filter, gave degradation rates up to 10 kg COD/m3d at loading rates of 15 kg COD/m3d and hydraulic retention time (HRT) of 3.1 hours. The anaerobic multi-stage reactor, consisting of three compartments, each packed with granular sludge and carrier elements, gave degradation rates up to 9 kg COD/m3d at loading rates of 15-16 kg COD/m3d, and HRT down to 2.6 hours. Clogging and short circuiting eventually became a problem in the multi-stage reactor, probably caused by too high packing of the carriers. The anaerobic moving bed biofilm reactor performed similar to the other reactors at loading rates below 1.4 kg COD/m3d, which was the highest loading rate applied. The use of carriers in the anaerobic reactors allowed short HRT with good treatment efficiencies for TMP whitewater.

Nitrogen removal from livestock and poultry breeding wastewaters using a novel sequencing batch biofilm reactor

2010 ◽  
Vol 62 (11) ◽  
pp. 2599-2606 ◽  
Author(s):  
Hong Xiao ◽  
Ping Yang ◽  
Hong Peng ◽  
Yanzong Zhang ◽  
Shihuai Deng ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Heterotrophic Bacteria ◽  
Biofilm Reactor ◽  
N Accumulation ◽  
Sequencing Batch Biofilm Reactor ◽  
Poultry Breeding ◽  
Mode 2 ◽  
Do Concentration ◽  
Nitrification And Denitrification ◽  
Biological Nitrogen

A study was conducted regarding the biological nitrogen removal from the livestock and poultry breeding wastewater (LPBWs) using a novel sequencing batch biofilm reactor (SBBR). Nitrogen removal process was studied under three aeration strategies/modes, referred to as MODE 1, 2, and 3. The results showed that MODE 2 (one operation period: instant fill of LPBWs, 3.0 h aeration, 1.5 h non-aeration, 1.5 h aeration, 1.0 h non-aeration and rapid drain of treated LPBWs) performed the best in nitrogen removal. Under MODE 2, the removal efficiencies were as high as 96.1 and 92.1% for NH3-N and TN, respectively. Simultaneous nitrification and denitrification (SND), as well as shortcut nitrification and denitrification are likely to be the two main mechanisms for the nitrogen removal in this study. Nitrifying bateria were not inhibited by heterotrophic bacteria with C/N ratios ranging from 18.1 to 21.4 and DO concentration of 2.0 mg/l. Alternation between aeration and non-aeration played an important role in NO2−-N accumulation.

Modeling the performance of biodegradation of textile wastewater using polyurethane foam sponge cube as a supporting medium

2010 ◽  
Vol 62 (12) ◽  
pp. 2801-2810 ◽  
Author(s):  
Yen-Hui Lin
Keyword(s):  
Steady State ◽  
Polyurethane Foam ◽  
Textile Wastewater ◽  
Pilot Scale ◽  
Biofilm Reactor ◽  
Column Test ◽  
Steady State Condition ◽  
Suspended Biomass ◽  
The Government ◽  
Carbon Dioxide Co2

A pilot-scale fixed-biofilm reactor (FBR) was established to treat textile wastewater to evaluate the feasibility of replacing conventional treatment processes that involve activated sludge and coagulation units. A kinetic model was developed to describe the biodegradation of textile wastewater by FBR. Batch kinetic tests were performed to evaluate the biokinetic parameters that are used in the model. FBR column test was fed with a mean COD of 692 mg/L of textile wastewater from flow equalization unit. The influent flow rate was maintained at 48.4 L/h for FBR column test. Experimental data and model-predicted data for substrate effluent concentration (as COD), concentration of suspended biomass in effluent and the amount of carbon dioxide (CO2) produced in the effluent agree closely with each other. Microscopic observations demonstrated that the biofilm exhibited a uniform distribution on the surface of polyurethane foam sponge. Under a steady-state condition, the effluent COD from FBR was about 14.7 mg COD/L (0.0213 Sb0), meeting the discharge standard (COD < 100 mg/L) that has been set by the government of Taiwan for textile wastewater effluent. The amount of biofilm and suspended biomass reached a maximal value in the steady state when the substrate flux reached a constant value and remained maximal. Approximately 33% of the substrate concentration (as COD) was converted to CO2 during biodegradation in the FBR test. The experimental and modeling schemes proposed in this study could be employed to design a full-scale FBR to treat textile wastewater.

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