Results of compact facility work with anaerobic treatment of highly concentrated runoffs

This scientific work is devoted to the results of the local treatment facilities’ work for food enterprises sewerage. The complex under consideration is intended for the anaerobic processing of whey, formed during fermented milk production. Whey is one of the specific organic substances, which main component is lactose (sucrose). It is converted under anaerobic conditions into lactic acid, which is a metabolic dead end. The capacity can be used as a homogenizing tank, obtained in this way. In the future, it is proposed to increase the homogenizer volume to 100 m3. It is recommended to use ammonium hydroxide or sulfate to ensure the required ratio between COD and biogenic element (nitrogen) in the loaded whey. Possible biogenic additives can be sewage sludge or slurry. The experience of adjustment was shown that slurry from cattle farms was quite simple, inexpensive and quite effective. Slurry can be injected in 3-4 m3 every 2-3 days. The stability of pH values in the bioreactor can be ensured by the correct placement of sensors for monitoring this indicator and by changing the method of alkali introduction. It is recommended to install pH control sensors at the bottom of the bioreactor, instead of whey loading pipeline. In order to provide for fractional injection of alkali, we should introduce part of the alkali at the homogenization stage, and part directly into the bioreactor. For an unimpeded build-up of anaerobic biomass, it is recommended to replace the centrifuge thickener operating with flocculant with the classic version of thickening in a compactor without flocculant adding.

A New Biorefinery Approach for the Full Valorisation of Anchovy Residues: Use of the Sludge Generated during the Extraction of Fish Oil as a Nitrogen Supplement in Anaerobic Digestion

Several anchovies species are captured all over the world; they are consumed fresh but also preserved by the industry, either by brine-fermentation or canning in oil. The industrial process generates large amounts of residue (about 50% of the original fish biomass) that is generally used to produce fish flour. In this paper, the advancement of a recently proposed process for the full valorisation of anchovies aimed at the extraction of fish oil (to be used as an omega-3 source) and at the production of biomethane through anaerobic digestion is presented. Particularly, in the experiments presented, a co-digestion of anchovy sludge—used as a nitrogen supplement—and market waste (5% and 95% on a Total Solids basis) was performed. Since the proposed extraction process uses, as a green-solvent, d-limonene, the well-known problems of toxicity for the anaerobic biomass must be overcome during the digestion process. As discussed below, the granular activated carbon (GAC) is used to reclaim and improve anaerobic digestion processes in a reactor displaying clear signs of inhibition. In fact, GAC demonstrates multiple benefits for anaerobic digestion, such as adsorption of toxic substances, biomass selection, and triggering of direct interspecies electron transfer (DIET).

Complete Genome Sequence of a New Bacteroidaceae Bacterium Isolated from Anaerobic Biomass Digestion

Here, we present the genome sequence and annotation of HV4-6-C5C, a bacterial strain isolated from a mesophilic two-stage laboratory-scale leach bed biogas reactor system. Strain HV4-6-C5C may represent a new genus of the family Bacteroidaceae and may have a key role in acidogenesis and acetogenesis steps during anaerobic biomass digestion.

Treatment of poultry slaughterhouse wastewater using a down-flow expanded granular bed reactor

This study evaluated the performance of a novel high rate anaerobic bioreactor system for the treatment of poultry slaughterhouse wastewater (PSW). The new system consisted of a granule-based technology operated in a down-flow configuration, with the assistance of medium-sized pumice stones used as packing materials for the retention of the anaerobic granules, to avoid challenges associated with the use of the three-phase separator of up-flow systems and the washout of the anaerobic biomass. Furthermore, a recycling stream was applied to the system to improve the mixing inside the Down-flow Expanded Granular Bed Reactor (DEGBR), i.e. the influent distribution to the granular biomass, and the implementation of intermittent fluidization when required to alleviate the effects of pressure drop in such systems. The DEGBR was operated under mesophilic conditions (30–35 °C) and achieved total chemical oxygen demand (tCOD), five-day biological oxygen demand and total suspended solids average removal percentages >95%, and a fats, oils and grease average removal percentage of 93.67% ± 4.51, for an organic loading rate varying between 1.1 to 38.9 gCOD/L.day.

Complete Genome Sequence of a New Ruminococcaceae Bacterium Isolated from Anaerobic Biomass Hydrolysis

ABSTRACT A new Ruminococcaceae bacterium, strain HV4-5-B5C, participating in the anaerobic digestion of grass, was isolated from a mesophilic two-stage laboratory-scale leach bed biogas system. The draft annotated genome sequence presented in this study and 16S rRNA gene sequence analysis indicated the affiliation of HV4-5-B5C with the family Ruminococcaceae outside recently described genera.

Complete Genome Sequence of a New Firmicutes Species Isolated from Anaerobic Biomass Hydrolysis

ABSTRACT A new Firmicutes isolate, strain HV4-6-A5C, was obtained from the hydrolysis stage of a mesophilic and anaerobic two-stage lab-scale leach-bed system for biomethanation of fresh grass. It is assumed that the bacterial isolate contributes to plant biomass degradation. Here, we report a draft annotated genome sequence of this organism.