Regulation of Saccharomyces cerevisiae maltose fermentation by cold temperature and CSF1

We describe the isolation of a 22.6-kilobase fragment of DNA containing the MAL1 locus of Saccharomyces cerevisiae. Our results demonstrate that the MAL1 locus, like the MAL6 locus, is a complex locus containing three genes. These genes were organized similarly to their MAL6 counterparts. We refer to them as MAL11, MAL12, and MAL13 and show that they are functionally homologous to the MAL61 (encoding maltose permease), MAL62 (encoding maltase), and MAL63 (encoding the positive regulator) genes of the MAL6 locus. Transcription from each of the three genes was analyzed in a strain carrying the undisrupted MAL1 locus and in strains carrying single disruptions in each of the MAL1 genes. The MAL1 and MAL1 loci were found to be highly sequence homologous and conserved throughout the region containing these three genes. The strain used to isolate the MAL1 locus also carried the tightly linked SUC1 gene. The SUC1 gene was found to be located on the same 22.6-kilobase fragment containing the MAL1 locus and 5 kilobases from the 3' end of the MAL12 gene. The meaning of these results with regard to the mechanism of regulation of maltose fermentation is discussed.

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Structural and functional analysis of the MAL1 locus of Saccharomyces cerevisiae.

Molecular and Cellular Biology ◽

10.1128/mcb.6.11.3891 ◽

1986 ◽

Vol 6 (11) ◽

pp. 3891-3899 ◽

Cited By ~ 61

Author(s):

M J Charron ◽

R A Dubin ◽

C A Michels

Keyword(s):

Saccharomyces Cerevisiae ◽

Functional Analysis ◽

Maltose Permease ◽

Positive Regulator ◽

Maltose Fermentation ◽

Complex Locus ◽

Regulator Genes

We describe the isolation of a 22.6-kilobase fragment of DNA containing the MAL1 locus of Saccharomyces cerevisiae. Our results demonstrate that the MAL1 locus, like the MAL6 locus, is a complex locus containing three genes. These genes were organized similarly to their MAL6 counterparts. We refer to them as MAL11, MAL12, and MAL13 and show that they are functionally homologous to the MAL61 (encoding maltose permease), MAL62 (encoding maltase), and MAL63 (encoding the positive regulator) genes of the MAL6 locus. Transcription from each of the three genes was analyzed in a strain carrying the undisrupted MAL1 locus and in strains carrying single disruptions in each of the MAL1 genes. The MAL1 and MAL1 loci were found to be highly sequence homologous and conserved throughout the region containing these three genes. The strain used to isolate the MAL1 locus also carried the tightly linked SUC1 gene. The SUC1 gene was found to be located on the same 22.6-kilobase fragment containing the MAL1 locus and 5 kilobases from the 3' end of the MAL12 gene. The meaning of these results with regard to the mechanism of regulation of maltose fermentation is discussed.

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Detection of maltose fermentation genses in the baking yeast strains of Saccharomyces cerevisiae

Letters in Applied Microbiology ◽

10.1111/j.1472-765x.1996.tb00080.x ◽

1996 ◽

Vol 23 (4) ◽

pp. 266-268 ◽

Cited By ~ 11

Author(s):

Y. Oda ◽

K. Tonomura

Keyword(s):

Saccharomyces Cerevisiae ◽

Maltose Fermentation ◽

Yeast Strains

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THE EFFECTS OF THREE DIFFERENT MAL LOCI ON THE REGULATION OF MALTASE SYNTHESIS IN YEAST

Genetics ◽

10.1093/genetics/98.4.713 ◽

1981 ◽

Vol 98 (4) ◽

pp. 713-728

Author(s):

Deborah Bernhardt Mowshowitz

Keyword(s):

Saccharomyces Cerevisiae ◽

Inbred Strains ◽

Regulatory Genes ◽

Maltose Fermentation ◽

Kinetics Of

ABSTRACT Inbred haploid strains of Saccharomyces cerevisiae carrying MAL1, MAL2 or MAL6 in a common background have been crossed to each other and to strains carrying no active MAL loci. The kinetics of maltase induction and the induced maltase levels have been examined in the inbred strains and in haploid segregants of the crosses. Differences have been found in the kinetics of induction and induced maltase levels that segregate with the different MAL loci. In the strains tested, the relative rates of maltase induction were MAL2>MAL6>>MAL1; the relative induced maltase levels were MAL2>MAL6~MAL1. These results indicate that MAL1, MAL2 and MAL6 are (or include) regulatory genes that control the accumulation of the enzymes of maltose fermentation.

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