scholarly journals Expression of Sec61alpha in F9 and P19 teratocarcinoma cells after retinoic acid treatment

2003 ◽  
Vol 63 (2) ◽  
pp. 245-252 ◽  
Author(s):  
L. R. Ferreira ◽  
C. E. E. Velano ◽  
E. C. Braga ◽  
C. C. Paula ◽  
H. Martélli-Junior ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Acid Treatment ◽  
Secretory Proteins ◽  
P19 Cells ◽  
Retinoic Acid Treatment ◽  
Teratocarcinoma Cell ◽  
F9 Cells ◽  
Teratocarcinoma Cells ◽  
Differentiation Stage

Nascent procollagen peptides and other secretory proteins are transported across the endoplasmic reticulum (RE) membrane through a protein-conducting channel called the translocon. Sec61alpha, a multispanning membrane translocon protein, has been implicated as essential for translocation of polypeptides chains into the cisterns of the ER. However, it is not known whether Sec61alpha is ubiquitously expressed in collagen producing teratocarcinoma cells. Furthermore, the production, expression, and utilization of Sec61alpha may depend on the cell differentiation stage. Stem cells from many cultured teratocarcinoma cell lines such as F9 and P19 cells are capable of differentiation in response to low retinoic acid concentrations. This differentiation of the tumorigenic stem cells results in tumorigenicity loss. For this study, mouse F9 and P19 teratocarcinoma cells were grown in culture medium treated with or without retinoic acid. Expression of Sec61alpha was determined by reverse trancriptase polimerase chain reaction (RT-PCR). In untreated conditions, F9 cells expressed undetected Sec61alpha amounts. It was also demonstrated that Sec61alpha expression is stimulated in F9 cells after retinoic acid treatment for 72 hours. No changes were found in Sec61alpha expression in P19 cells after retinoic acid treatment. These data indicate that the expression of Sec61alpha is enhanced with retinoic acid induced differentiation of F9 teratocarcinoma cells.

Retinoic acid treatment enhances the acetylcholine contents in the human teratocarcinoma cell line NTera-2

2000 ◽  
Vol 96 (1-2) ◽  
pp. 59-63 ◽  
Author(s):  
Hanspeter S. Fischer ◽  
Irene Berti ◽  
Dieter S. Schatz ◽  
Christian Humpel ◽  
Alois Saria
Keyword(s):  
Retinoic Acid ◽  
Cell Line ◽  
Acid Treatment ◽  
Retinoic Acid Treatment ◽  
Teratocarcinoma Cell ◽  
Teratocarcinoma Cell Line

scholarly journals Emergence of undifferentiated colonies from mouse embryonic stem cells undergoing differentiation by retinoic acid treatment

2016 ◽  
Vol 52 (5) ◽  
pp. 616-624 ◽  
Author(s):  
Lioudmila V. Sharova ◽  
Alexei A. Sharov ◽  
Yulan Piao ◽  
Carole A. Stagg ◽  
Tomokazu Amano ◽  
...  
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Embryonic Stem Cells ◽  
Acid Treatment ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Retinoic Acid Treatment

scholarly journals Lamp-1 does not acquire the large polylactosaminoglycans characteristic of F9 cells

1993 ◽  
Vol 296 (1) ◽  
pp. 253-257 ◽  
Author(s):  
P A Romero ◽  
T Way ◽  
A Herscovics
Keyword(s):  
Monoclonal Antibodies ◽  
Retinoic Acid ◽  
Membrane Protein ◽  
Acid Treatment ◽  
Datura Stramonium ◽  
Lysosomal Membrane ◽  
Retinoic Acid Treatment ◽  
F9 Cells ◽  
Lysosomal Membrane Proteins ◽  
Lysosomal Membrane Protein

Although F9 cells labelled with [3H]glucosamine synthesize many glycoproteins that bind to Datura stramonium agglutinin-agarose, only a small proportion of these were immunoprecipitated with monoclonal antibodies to lamp-1 and lamp-2 (lamp = lysosomal membrane protein). Differentiation of F9 cells by retinoic acid increased labelling of all Datura stramonium-bound glycoproteins, including lamp-1 and lamp-2. Although the large polylactosaminoglycans excluded from Bio-Gel P-6 that are characteristic of F9 cells were obtained from total glycoproteins, little of these large polylactosaminoglycans was found on lamp-1 and lamp-2. There was no increase in large polylactosaminoglycans of lamp-1 and lamp-2 after retinoic acid treatment, but an increase in the size of small polylactosaminoglycans (included on Bio-Gel P-6) and tri- and tetra-antennary complex oligosaccharides. Therefore, other factors besides the expression of specific glycosyltransferases determine the extent of elongation of polylactosaminoglycans on lysosomal membrane proteins.

An octamer motif contributes to the expression of the retinoic acid-regulated zinc finger gene Rex-1 (Zfp-42) in F9 teratocarcinoma cells

1993 ◽  
Vol 13 (5) ◽  
pp. 2919-2928
Author(s):  
B A Hosler ◽  
M B Rogers ◽  
C A Kozak ◽  
L J Gudas
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Zinc Finger ◽  
Chromosome 8 ◽  
Lacz Gene ◽  
F9 Cells ◽  
Zinc Finger Gene ◽  
Teratocarcinoma Cells ◽  
Octamer Motif ◽  
F9 Teratocarcinoma

The message for the zinc finger gene Rex-1 (Zfp-42) is expressed in undifferentiated murine F9 teratocarcinoma cells and embryonic stem cells. Expression of Rex-1 is reduced at the transcriptional level when F9 cells are induced by the addition of retinoic acid (RA) to differentiate. We have isolated genomic DNA for the Rex-1 gene (Zfp-42), characterized the gene's structure, and mapped the gene to mouse chromosome 8. Promoter elements contributing to the regulation of the Rex-1 promoter in F9 cells have been identified. A region required for Rex-1 promoter activity in F9 stem cells contains an octamer motif (ATTTGCAT) which is a binding site for octamer transcription factor members of the POU domain family of DNA-binding proteins. Rex-1 reporter plasmids including this octamer site also exhibited reduced expression in F9 cells treated with RA. Thus, the octamer motif is a regulatory element required for the activity of the Rex-1 promoter in F9 stem cells, and this motif contributes to the negative regulation by RA of the transcription of the Rex-1 gene. As an initial confirmation of the in vivo relevance of the isolated fragment, a larger Rex-1 promoter fragment, also containing the octamer site, was able to promote expression of the bacterial lacZ gene in mouse embryos at the morula stage.

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