scholarly journals Antibacterial effect of root canal preparation and calcium hydroxide paste (Calen) intracanal dressing in primary teeth with apical periodontitis

2005 ◽  
Vol 13 (4) ◽  
pp. 351-355 ◽  
Author(s):  
Gisele Faria ◽  
Paulo Nelson-Filho ◽  
Aldevina Campos de Freitas ◽  
Sada Assed ◽  
Izabel Yoko Ito

The aim of this study was to evaluate the antibacterial action of root canal mechanical preparation using 2.5% sodium hypochlorite as the irrigating solution and a calcium hydroxide paste as the antibacterial intracanal dressing in human primary teeth root canals with pulp necrosis and apical periodontitis by means of microbial culture. A total of 26 root canals of human primary teeth with pulp necrosis and apical periodontitis were used. Samples were collected before, 72h after biomechanical treatment and 72h after removal of the intracanal dressing. Comparison by Wilcoxon test showed that root canal mechanical preparation effectively eliminated all microorganisms in 20% of the root canals, and the intracanal dressing in 62.5%; however, the cumulative action of biomechanical treatment and intracanal dressing eliminated the microorganisms of 70% of the root canals (p<0.001). Isolated root canal mechanical preparation presented poorer microbiological results that those obtained with root canal mechanical preparation and the use of an intracanal dressing indicating the necessity of topical application of an intracanal medication between sessions in primary teeth with pulp necrosis and apical periodontitis.

2020 ◽  
Vol 9 (3) ◽  
pp. 854 ◽  
Author(s):  
Alexandre P. L. Carvalho ◽  
Laura C. L. Nardello ◽  
Fernanda S. Fernandes ◽  
Fernanda P. Bruno ◽  
Luiza R. Paz ◽  
...  

New tools for activating endodontic irrigants have evolved, yet their impact on root canal disinfection, in comparison to the passive placing of an inter-visit medication, have not yet been fully elucidated. The use of DNA- and rRNA-based methods may cast some new light on this issue, as they allow a comparison to be made between microbial presence and activity. Therefore, the aim of this single-arm intervention trial is to evaluate the antibacterial effect of endodontic procedures using both molecular methods. Root canal samples were obtained from 20 patients with asymptomatic apical periodontitis after each treatment step: access cavity, chemo-mechanical preparation, adjunctive procedures (XP-endo Finisher file and passive ultrasonic irrigation), calcium hydroxide medication, and 2nd-visit root canal preparation. DNA and cDNA from the samples were subjected to quantitative polymerase chain reaction with universal primers for the bacterial 16S rRNA gene. Chemo-mechanical preparation promoted a drastic reduction in bacterial levels and activity, whereas the adjunctive procedures did not make a significant contribution to further disinfection. At the 2nd visit, bacteria were active after the use of calcium hydroxide medication; however, they were significantly reduced after a 2nd-visit preparation. Consequently, the lowest bacterial levels were found at the end of the treatment. This clinical trial, which used an rRNA and rDNA combined approach, confirmed previous studies showing that root canal preparation represents the main strategy for root canal disinfection.


2018 ◽  
Vol 42 (2) ◽  
pp. 146-149
Author(s):  
Fadi Said ◽  
Moti Moskovitz

Objectives: The aim of the present study was to assess the effect of calcium hydroxide as a root canal dressing material on dentin microtensile fracture strength in human primary teeth in vitro. Study design: Thirty primary anterior teeth with root canals packed with calcium hydroxide were divided into groups of ten and immersed in saline at room temperature for 7, 30 and 90 days. Ten teeth with root canals filled with sterile saline were the control group. Microtensile fracture strength was measured in Mechanical tester Lloyd testing machine. Results: There was a significant difference (P &lt; 0.05) between the fracture strength of the calcium hydroxide-filled teeth after 90 days (19.1 MPa) compared with the control (35.8 MPa). Dentin microtensile fracture strength of the calcium hydroxide-filled teeth decreased at an average of 0.142 MPa per day. Conclusion: Calcium hydroxide placed in root canals for an extended time had a significantly negative effect on root strength. Long-term success of root canal treatment in primary anterior teeth is estimated as 65% with most of the failures result from trauma recurrence. Clinical Relevance: Our results stress the need to evaluate the pros and cons of root canal treatment compared to extractions of non-vital primary incisors.


2002 ◽  
Vol 49 (1-2) ◽  
pp. 53-56
Author(s):  
Tatjana Brkanic ◽  
Slavoljub Zivkovic

The aim of this paper was to, on the basis of everyday clinical practice analize the effect of a homeopathic remedy, as a complementary remedy in endodontic therapy of endoperiodontal lesions and chronic apical periodontitis and to radiologically check its effect on the flow and speed of reparation of damaged periodontal tissues. Endodontic therapy was done according to the usual protocol for the therapy of infectious canals with root canal preparation, medication with calcium hydroxide points and hermetic obturation with AH-26 paste. During endodontic treatment patients were taking homeophatic remedy Hepar sulfuris per os three times daily. The remedy stimulates the natural defences mechanism of the organism. Radiological follow up after three months period showed obvious reductiont of radiologic radiolucency in periapical region.


2018 ◽  
Vol 24 (4) ◽  
pp. 164-167
Author(s):  
Gheorghiu Irina-Maria ◽  
Mitran Loredana ◽  
M. Mitran ◽  
Temelcea Anca-Nicoleta ◽  
Scarlatesc Sanziana ◽  
...  

Abstract The aim of this study was to evaluate the in vivo efficiency of endodontic treatment with calcium hydroxide in chronic apical periodontitis by determining the microbial load from the endodontic space in different stages of treatment. The microbiological determinations that have been made have led to the conclusion that calcium hydroxide possesses a redoubltable antimicrobial activity, but only with the condition of long-lasting contact with the root canals. Another important result is the essential role of the rigorous biomechanical treatment of the root canals, intracanal medication with calcium hydroxide is addresses mostly to microorganisms located in root canal ramifications.


2011 ◽  
Vol 21 (5) ◽  
pp. 353-360 ◽  
Author(s):  
IZABEL YOKO ITO ◽  
FUMIO MATOBA JUNIOR ◽  
FRANCISCO WANDERLEY GARCIA PAULA-SILVA ◽  
LÉA ASSED BEZERRA Da SILVA ◽  
MÁRIO ROBERTO LEONARDO ◽  
...  

2020 ◽  
Vol 31 (5) ◽  
pp. 493-498
Author(s):  
Mônica A. S. Neves ◽  
José C. Provenzano ◽  
Simone C. L. Fonseca ◽  
Renata C. V. Rodrigues ◽  
Lucio S. Gonçalves ◽  
...  

Abstract This clinical study reports on the antibacterial effects and outcome of endodontic treatment using either a single-file or a multifile system, associated with calcium hydroxide interappointment medication. The root canals of single-rooted teeth with apical periodontitis were treated by using either Reciproc or BioRaCe instrument systems, 2.5% NaOCl irrigation, and calcium hydroxide medication. Bacteriological samples taken before preparation and immediately before obturation were evaluated for total bacterial counts by quantitative real-time polymerase chain reaction (qPCR). Patients were followed up and the treatment outcome was assessed by clinical and radiographic criteria. Decreasing lesions were classified as success in a lenient criterion or failure in a rigid one. Bacteria were detected in all initial samples (47 cases) and were significantly reduced after treatment in both groups (p<0.001). In the Reciproc and BioRaCe groups, 7/25 (28%) and 11/22 (50%) root canals yielded negative qPCR results before obturation, respectively (p>0.05). Quantitative bacterial reduction was similar between groups (p>0.05). The success rate in the BioRaCe group was 95.5% and 77% in the loose and rigid criterion, respectively. In the Reciproc group, corresponding figures were 88% and 76%. Differences in outcome were not significant (p>0.05). No diseased case showed negative qPCR results for bacteria. A difference of >1 Log10 counts was observed between healed and diseased cases. Root canal treatments of teeth with apical periodontitis using a single-file or a multifile system for preparation, associated with NaOCl irrigation and calcium hydroxide interappointment medication, showed similar antibacterial effectiveness and success rate.


2009 ◽  
Vol 20 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Patricia Elaine Panicali Lana ◽  
Miriam Fatima Záccaro Scelza ◽  
Licínio Esmeraldo Silva ◽  
Ana Luíza de Mattos-Guaraldi ◽  
Raphael Hirata Júnior

The effectiveness of calcium hydroxide pastes: Calen™ and PMCC-Calen™ associated to chemo-mechanical preparation was assessed on Enterococcus faecalis grown within root canals. Seventy incisors were inserted into TSB medium, sterilized and contaminated with E. faecalis. Culture medium was replaced each 24 h and incubated at 37oC for 72 h. After chemo-mechanical preparation, root canals were filled with Calen™ or PMCC-Calen™ (7 or 14 days). Pastes were removed and teeth were inserted into test tubes containing Enterococcosel broth. Calen™ paste (maintained for 7 and 14 days) induced 70% elimination of enterococci and PMCC-Calen™ 100% elimination only after maintenance for 14 days. These medications were significantly more effective (p<0.001) than chemo-mechanical protocol alone and PMCC-Calen™ maintained for 7 days, both incapable to eliminate the viability of enterococci. Calcium hydroxide pastes demonstrated important adjuvant effects in the elimination of enterococci during chemo-mechanical preparation of root canal systems. When associated with PMCC, calcium hydroxide pastes should be maintained for at least 14 days.


2010 ◽  
Vol 35 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Priya Harini ◽  
Sham Bhat ◽  
K Sundeep Hegde

Background and objectives: Since complete debridement of the root canals of the primary teeth is not practically possible due to the highly variable root canal anatomy, success of the endodontic therapy depends partly on the use of antibacterial irrigating agents and root canal filling materials. Recent literature indicates that anaerobes comprise a majority of the bacteria in necrotic root canals of primary teeth. The study determined the antibacterial effectiveness of four root canal filling materials namely Calcium hydroxide,Zinc oxide eugenol, Vitapex and Metapex against microbial specimens obtained directly from necrotic root canals of primary teeth. Method: Microbial specimens were collected using sterile paper points, from 15 primary maxillary and mandibular posterior teeth of randomly selected children in the age group of 4-10 years with infected non vital primary teeth, requiring pulpectomy procedure. The microbial specimens collected were subjected to microbiological analysis and the antimicrobial potential of root canal filling materials were tested using Agar diffusion technique. Results: were statistically analyzed using one-way ANOVA. Facultative/Aerobic organisms were isolated in all the cases, anaerobic organisms were isolated in 80% of the cases, and Candida albicans was isolated in 1 case. ZOE showed superior inhibitory activity against most of the organisms isolated followed by Vitapex, Calcium hydroxide and Metapex in descending order.Conclusion: Our data may be useful as a guide for relative antimicrobial effectiveness or non-effectiveness of the materials employed. In vivo studies are required to state the specific antimicrobial activity and merits and demerits of any of the test filling material.


2017 ◽  
Vol 29 (3) ◽  
Author(s):  
Anna Muryani ◽  
Ayu Trisna Hayati ◽  
Rahmi Alma Farah Adang

Introduction: Irrigation of the root canal is an important part of the endodontic treatment. Root irrigation technique can be done with the manual and sonic system by using 2.5% NaOCI solution. Calcium hydroxide [Ca(OH)2 ] is used as a medicament for root canal sterilization. Root canal treatment will fail due to the imperfect removal of Ca(OH)2 residue. The objective of this research was to analyze the comparison of the removal of Ca(OH)2 medicaments on the root canal treatment irrigated with manual and sonic agitation technique using 2.5% NaOCI. Methods: The methods used in this study was experimental laboratory. The sample used was 30 maxillary incisors. The teeth were then divided into two groups randomly, then the root canal preparation was done by the crown down technique with irrigation using 2.5% NaOCI. The radicular part of the teeth was then split longitudinally, given a standardized groove in the one-third of the apical part, then applied with water-solved calcium hydroxide. The teeth were unified afterwards by using flowable composites, then soaked in the artificial saliva. The sample of the 1st group was irrigated by manual agitation technique, and the 2nd group by sonic agitation technique. The data results were analyzed by Kruskal-Wallis and Mann-Whitney tests. Results: The results of Ca(OH)2 removal were different between manual agitation technique compared to the sonic agitation technique. Conclusion: The irrigation using 2.5% NaOCl with the sonic agitation technique were proven to be more effective in removing Ca(OH)2 from root canals than the manual agitation technique.


Author(s):  
O. A Pavlovich ◽  
L. A Skorikova ◽  
N. P Bazhenova ◽  
N. V Lapina

Increase of efficiency of treatment of patients with chronic apical periodontitis is a priority in dentistry. A root canal is a breeding ground for microorganisms. The main reason for unfavourable treatment outcomes of teeth with chronic apical periodontitis is incomplete debridement of the root canal system. Optimizing the treatment of patients with chronic periodontitis with the use of complex drugs galodent No. 4 (clotrimazole, tracert and calcium hydroxide) is a priority in dentistry. As a result of researches it is established that the antibacterial effect of the temporary investment of complex drugs galodent No. 4 and clotrimazole in the root canals of teeth with chronic apical periodontitis is 2,9 times higher and the proportion of positive results is, on average, 1,5 times higher compared to the practiced methods in the comparison groups


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