Nomenclature Abstract for Propionibacterium thoenii van Niel 1928 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Propionibacterium Thoenii

Long-Term Storage Stability of the Bacteriocin Propionicin PLG-1 Produced by Propionibacterium thoenii and Potential as a Food Preservative†

1996 ◽  
Vol 59 (5) ◽  
pp. 481-486 ◽  
Author(s):  
HSING-YI HSIEH ◽  
BONITA A. GLATZ
Keyword(s):  
Storage Stability ◽  
Skim Milk ◽  
Lactobacillus Delbrueckii ◽  
Food Preservative ◽  
Long Term Storage ◽  
Cell Counts ◽  
Viable Cells ◽  
Propionibacterium Thoenii ◽  
Term Storage

Propionicin PLG-1, a bacteriocin produced by Propionibacterium thoenii strain P127, was tested for characteristics that could determine its usefulness as a food preservative: long-term storage stability and effectiveness in a food model system. Partially purified propionicin PLG-1 samples, lyophilized and nonlyophilized, were stored at 25, 4, and −20°C. Bacteriocin activity increased by as much as 200% over the first 10 days of storage in nonlyophilized samples stored at 25 or 4°C. Activity then decreased gradually for samples stored at 25°C while samples stored at 4°C retained high activity through 14 weeks of storage. Nonlyophilized samples frozen at −20°C and lyophilized samples stored at all temperatures did not change significantly in activity through 25 weeks of storage. Propionicin was added at 100 and 1,000 arbitrary units (AU)/ml to lactobacilli MRS broth and to skim milk, each inoculated with 105 cells per ml of Lactobacillus delbrueckii ATCC 4797. Upon incubation at 37°C with 1,000 AU/ml, cell numbers were reduced by at least 4 log units within 2 h and no viable cells were detected after 96 h in either medium. With 100 AU/ml of propionicin, viable cells were reduced by 2 log units within 12 h at 37°C, but culture growth resumed after 24 h. At 15°C, no viable cells were detected after 48 h in the presence of 1,000 AU/ml of propionicin, while viable cell counts were gradually reduced to about 10 cells per ml by 168 h in the presence of 100 AU/ml of propionicin.

scholarly journals Biochemical and Genetic Characterization of Propionicin T1, a New Bacteriocin from Propionibacterium thoenii

2000 ◽  
Vol 66 (10) ◽  
pp. 4230-4236 ◽  
Author(s):  
Therese Faye ◽  
Thor Langsrud ◽  
Ingolf F. Nes ◽  
Helge Holo
Keyword(s):  
Amino Acids ◽  
Stop Codon ◽  
Sequence Similarity ◽  
Propionibacterium Freudenreichii ◽  
Terminal Part ◽  
Reading Frame ◽  
Lactobacillus Sake ◽  
Propionibacterium Thoenii ◽  
Self Protection

ABSTRACT A collection of propionibacteria was screened for bacteriocin production. A new bacteriocin named propionicin T1 was isolated from two strains of Propionibacterium thoenii. This bacteriocin shows no sequence similarity to other bacteriocins. Propionicin T1 was active against all strains of Propionibacterium acidipropionici, Propionibacterium thoenii, andPropionibacterium jensenii tested and also againstLactobacillus sake NCDO 2714 but showed no activity againstPropionibacterium freudenreichii. The bacteriocin was purified, and the N-terminal part of the peptide was determined with amino acid sequencing. The corresponding gene pctA was sequenced, and this revealed that propionicin T1 is produced as a prebacteriocin of 96 amino acids with a typical sec leader, which is processed to give a mature bacteriocin of 65 amino acids. An open reading frame encoding a protein of 424 amino acids was found 68 nucleotides downstream the stop codon of pctA. The N-terminal part of this putative protein shows strong similarity with the ATP-binding cassette of prokaryotic and eukaryotic ABC transporters, and this protein may be involved in self-protection against propionicin T1. Propionicin T1 is the first bacteriocin from propionibacteria that has been isolated and further characterized at the molecular level.

Improvement of Detection and Production of Propionicin PLG-1, a Bacteriocin Produced by Propionibacterium thoenii†

1996 ◽  
Vol 59 (7) ◽  
pp. 734-738 ◽  
Author(s):  
HSING-YI HSIEH ◽  
HYUN-DONG PAIK ◽  
BONITA A. GLATZ
Keyword(s):  
Corn Steep Liquor ◽  
Tween 80 ◽  
Sodium Lactate ◽  
Lactobacillus Delbrueckii ◽  
Base Layer ◽  
Beet Molasses ◽  
Controlled Conditions ◽  
Propionibacterium Thoenii ◽  
Steep Liquor ◽  
Diffusion Assay

Propionibacterium thoenii P127 produces propionicin PLG-1 in liquid culture at relatively low concentrations and slow production rates. The goal of this study was to increase the sensitivity and reproducibility of the standard well-diffusion assay for bacteriocin activity as well as to improve production of propionicin PLG-1 under controlled conditions in a fermenter. Several conditions were varied in the well-diffusion assay. The best results were obtained with 7-mm wells cut into a 5-mm-deep base layer that contained 2.5% agar, 0.85% NaCl and 0.1 % Tween 80. Plates were incubated at room temperature for 24 h or at 37°C for 2 h before adding bacteriocin samples to the wells to aid diffusion. Larger and clearer zones of inhibition were observed when Lactobacillus delbrueckii ATCC 4797 rather than Propionibacterium acidipropionici P5 was used as indicator strain, and results could be read in 12 h. Recovery of bacteriocin from the culture supernatant was improved by adding 0.1% Tween 80 to the buffer used for dialysis and resuspension of precipitated protein. Strain P127 was grown in six different media under controlled conditions in a fermenter: 12.5% beet molasses; 9% corn steep liquor; combinations of beet molasses and corn steep liquor at 1:3, 1:1, and 3:1 (vol:vol) ratios; and the standard growth medium, sodium lactate broth. Maximum production of propionicin PLG-1 was obtained in 3:1 beet molasses:corn steep liquor, and was 5 times greater than in sodium lactate broth.

Characterization of thoeniicin 447, a bacteriocin isolated from Propionibacterium thoenii strain 447

2004 ◽  
Vol 92 (2) ◽  
pp. 153-160 ◽  
Author(s):  
I.R. Van der Merwe ◽  
R. Bauer ◽  
T.J. Britz ◽  
L.M.T. Dicks
Keyword(s):  
Propionibacterium Thoenii

Two different propionicins produced by Propionibacterium thoenii P-127

Peptides ◽  
2003 ◽  
Vol 24 (11) ◽  
pp. 1733-1740 ◽  
Author(s):  
Galit Ben-Shushan ◽  
Varda Zakin ◽  
Natan Gollop
Keyword(s):  
Propionibacterium Thoenii

scholarly journals Development of a Propionibacterium-Escherichia coli Shuttle Vector for Metabolic Engineering of Propionibacterium jensenii, an Efficient Producer of Propionic Acid

2013 ◽  
Vol 79 (15) ◽  
pp. 4595-4602 ◽  
Author(s):  
Xin Zhuge ◽  
Long Liu ◽  
Hyun-dong Shin ◽  
Rachel R. Chen ◽  
Jianghua Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Propionic Acid ◽  
Shuttle Vector ◽  
Production Capacity ◽  
Glycerol Dehydrogenase ◽  
Sequencing Analysis ◽  
Chloramphenicol Resistance ◽  
Content Type ◽  
Propionibacterium Thoenii

ABSTRACTPropionic acid (PA) is an important chemical building block and is widely applied for organic synthesis, food, feedstuff, and pharmaceuticals. To date, the strains that can efficiently produce PA have includedPropionibacterium thoenii,P. freudenreichii, andP. acidipropionici. In this report, we show thatP. jenseniiATCC 4868 is also able to produce PA in much higher yields than the previously reported strains. To further improve the production capacity, aP. jensenii-Escherichia colishuttle vector was developed for the metabolic engineering ofP. jensenii. Specifically, a 6.9-kb endogenous plasmid, pZGX01, was isolated fromP. acidipropioniciATCC 4875 and sequenced. Since the sequencing analysis indicated that pZGX01 could encode 11 proteins, the transcriptional levels of the corresponding genes were also investigated. Then, aP. jensenii-Escherichia colishuttle vector was constructed using the pZGX01 plasmid, theE. colipUC18 plasmid, and a chloramphenicol resistance gene. Interestingly, not only could the developed shuttle vector be transformed intoP. jenseniiATCC 4868 and 4870, but it also could be transformed intofreudenreichiiATCC 6207 subspecies ofP. freudenreichii. Finally, the glycerol dehydrogenase gene (gldA) fromKlebsiella pneumoniaewas expressed inP. jenseniiATCC 4868 with the constructed shuttle vector. In a 3-liter batch culture, the PA production by the engineeredP. jenseniiATCC 4868 strain reached 28.23 ± 1.0 g/liter, which was 26.07% higher than that produced by the wild-type strain (22.06 ± 1.2 g/liter). This result indicated that the constructed vector can be used a useful tool for metabolic engineering ofP. jensenii.

scholarly journals Genus- and Species-Specific PCR-Based Detection of Dairy Propionibacteria in Environmental Samples by Using Primers Targeted to the Genes Encoding 16S rRNA

1999 ◽  
Vol 65 (9) ◽  
pp. 4241-4244 ◽  
Author(s):  
Franca Rossi ◽  
Sandra Torriani ◽  
Franco Dellaglio
Keyword(s):  
16S Rrna ◽  
Environmental Samples ◽  
Specific Pcr ◽  
Genes Encoding ◽  
Propionibacterium Thoenii ◽  
Pcr Assays ◽  
Dairy Propionibacteria ◽  
Species Specific

ABSTRACT PCR assays with primers targeted to the genes encoding 16S rRNA were developed for detection of dairy propionibacteria.Propionibacterium thoenii specific oligonucleotide PT3 was selected after partial resequencing. Tests allowed the detection of less than 10 cells per reaction from milk and cheese and 102 cells per reaction from forage and soil.

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