Biochemical composition of Porphyridium cruentum Nag. CNM-AR-01 (Rhodophyta) under culture conditions

2001 ◽  
Vol 3 (3) ◽  
pp. 63-68
Author(s):  
V. F. Rudik ◽  
L. E. Cepoi
Keyword(s):  
Biochemical Composition ◽  
Culture Conditions ◽  
Porphyridium Cruentum

scholarly journals Effects of Growth Medium Variation on the Nutri-Functional Properties of Microalgae Used for the Enrichment of Ricotta

2021 ◽  
Vol 60 (1) ◽  
Author(s):  
Sheyma Khemiri ◽  
Ines Bouchech ◽  
Nadia Berrejeb ◽  
Mondher Mejri ◽  
Issam Smaali ◽  
...  
Keyword(s):  
Functional Properties ◽  
Biochemical Composition ◽  
Radical Scavenging Activity ◽  
Dairy Product ◽  
Nitrogen Deficiency ◽  
Radical Scavenging ◽  
Biomass Productivity ◽  
Culture Conditions ◽  
Growth Media ◽  
Rich Media

Research background. Microalgae represent an emergent sustainable source of bioactive compounds such as antioxidants, vitamins, minerals, and polyunsaturated fatty acids that can ameliorate the nutritional characteristics of foods. The biochemical composition of microalgae could be modulated by varying the culture conditions to enhance the accumulation of biomolecules of interest. The aim of this work is to optimise the nutri-functional properties of two microalgae with potential utility for food-application. Experimental approach. Nannochloropsis gaditana.L2 and Chlorella sp.SM1 were screened for growth, biochemical composition, and radical scavenging activity employing four different growth media (Algal, BG-11, f/2, and Conway) with different nutrients composition. Additionally, the feasibility of using Chlorella sp.SM1 cultivated in BG-11 medium, in a Mediterranean under-investigated dairy product “ricotta cheese” and its effect on the sensory attributes was investigated. Results and conclusions. Nitrate- and phosphate-rich media (BG-11 and Algal) enhanced the biomass productivity. However, the highest lipid productivity (23.10 mg/(L·day); 11.86 mg/(L·day) for SM1 and L2 respectively) and carbohydrates content (34.79 %; 44.84 % for SM1 and L2 respectively) were obtained with the nitrate-deficient f/2 medium. Regardless of the used medium, the lipidic profile of Chlorella sp.SM1 and Nannochloropsis gaditana.L2 remained adequate for different applications with the presence of C16-18 as main fatty acid (>50 %). Significant increase in oleic acid (C18:1) content was recorded in response to nitrogen deficiency, being the highest in SM1 in f/2 medium (34 %). Nitrogen deficiency was also found to enhance phenolic compounds (48.8 GAE/(mg/g); 35.1 GAE/(mg/g) for SM1 and L2 respectively) and carotenoids contents (2.2 mg/g; 2 mg/g for SM1 and L2 respectively). Due to its interesting antioxidant potential, Chlorella sp.SM1 was used to enrich the ricotta cheese product at different concentrations (0.2 %, 1 % and 1.5 %). The sample with 0.2 % was found to give the most appreciated product. Novelty and scientific contribution. This study permitted the production of an innovative ricotta cheese using Chlorella, as a functional ingredient, without altering the manufacturing diagram while maintaining acceptable sensorial characteristics. The biochemical composition of the used strains varied depending on the culture media’s composition, which permitted the accumulation of phytonutrients of interest.

Lipid production in Porphyridium cruentum grown under different culture conditions

2009 ◽  
Vol 108 (5) ◽  
pp. 429-434 ◽  
Author(s):  
Sung Ho Oh ◽  
Jae Gun Han ◽  
Young Kim ◽  
Ji Hye Ha ◽  
Seung Seop Kim ◽  
...  
Keyword(s):  
Lipid Production ◽  
Culture Conditions ◽  
Porphyridium Cruentum

scholarly journals QTL Analysis of the Content of Some Bioactive Compounds in Brassica rapa L. Grown under Light Culture Conditions

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 583
Author(s):  
Ksenia V. Egorova ◽  
Nadezhda G. Sinyavina ◽  
Anna M. Artemyeva ◽  
Natalia V. Kocherina ◽  
Yuriy V. Chesnokov
Keyword(s):  
Molecular Markers ◽  
Brassica Rapa ◽  
Doubled Haploid ◽  
Biochemical Composition ◽  
Dry Matter ◽  
Culture Conditions ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
Doubled Haploid Lines

The article presents the results of biochemical and QTL (Quantitative Trait Loci) analysis of dry matter content, nutrient and biologically active compounds: sugars, ascorbic acid, chlorophylls a and b, anthocyanins and carotenoids in populations of doubled haploid lines of leaf, root crops, and oilseeds of the Brassica rapa L. species grown in optimal light culture conditions, but with different photoperiod durations. The purpose of this study was to evaluate the effect of the photoperiod on the transition to bolting and the accumulation of biologically active substances, as well as how the localization and identification of chromosomal loci determined the content of certain phytochemicals. The influence of the length of daylight hours on the content of components of the biochemical composition was assessed. It was shown that growing under conditions of a 16 h photoperiod increased the content of dry matter, sugars, vitamin C, and anthocyanins. On the contrary, the content of photosynthetic pigments was higher under the conditions of a 12 h photoperiod. Valuable lines that can be sources of biologically active compounds were revealed. Based on the results of the obtained data, 102 QTLs were mapped, which determine the manifestation of the studied biochemical quality traits in the B. rapa doubled haploid lines under conditions of short and long daylight hours. Molecular markers genetically linked to the selected QTLs were determined. It was revealed that the identified loci controlling all the studied biochemical traits were mainly in the fifth, sixth, seventh, and ninth linkage groups, which correlated with the data obtained in the field and greenhouse. Most of the identified loci controlled several studied traits simultaneously. The identified QTLs and identified molecular markers are of interest for further study of the genetic control of the economically valuable traits determined by them and for the implementation of marker-assisted selection in B. rapa. The data obtained can be used in genetic and breeding work, including for the obtaining of new genotypes, lines and cultivars with a valuable biochemical composition, adapted for cultivation under specific photoperiodic conditions.

scholarly journals GRANULES ASSOCIATED WITH THE CHLOROPLAST LAMELLAE OF PORPHYRIDIUM CRUENTUM

1966 ◽  
Vol 29 (3) ◽  
pp. 423-434 ◽  
Author(s):  
E. Gantt ◽  
S. F. Conti
Keyword(s):  
Red Algae ◽  
Close Association ◽  
Red Alga ◽  
Culture Conditions ◽  
Porphyridium Cruentum ◽  
Acetone Extraction

Small granules with a diameter of approximately 350 A are attached to the chloroplast lamellae of the red alga Porphyridium cruentum. To some extent, their size depends on the culture conditions and the age of the cell. It was possible to preserve the granules only with aldehyde prefixation. It can be seen that fixed or negatively stained granules are comprised of smaller subunits. The granules are arranged regularly on the lamellae in repeating rows with a center-to-center granule distance of 400 to 500 A. Attempts at characterization of these chloroplast granules revealed that they are resistant to hydrolysis by ribonuclease and appear to be structurally unaffected by methanol-acetone extraction. Because of their close association with the chloroplast lamellae, they are considered as possible sites of phycobilin concentration. This possibility is supported by two observations: when the phycobilins are removed, the granules disappear; and, when the chlorophyll and stainable membrane portions are selectively removed, the phycobilins and granules are still present. It was found that all other marine red algae examined had granules which were associated with the chloroplast lamellae.

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