scholarly journals Factors Influencing the Long-Term Behavior of Extracellular Matrix-Derived Scaffolds for Musculoskeletal Soft Tissue Repair

2012 ◽  
Vol 22 (3) ◽  
pp. 181-193 ◽  
Author(s):  
Christopher R. Rowland ◽  
Dianne Little ◽  
Farshid Guilak
Keyword(s):  
Extracellular Matrix ◽  
Soft Tissue ◽  
Tissue Repair ◽  
Soft Tissue Repair ◽  
Factors Influencing ◽  
Long Term Behavior

scholarly journals Further structural characterization of ovine forestomach matrix and multi-layered extracellular matrix composites for soft tissue repair

2021 ◽  
pp. 088532822110457
Author(s):  
Matthew J Smith ◽  
Sandi G Dempsey ◽  
Robert W Veale ◽  
Claudia G Duston-Fursman ◽  
Chloe A F Rayner ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Hyaluronic Acid ◽  
Soft Tissue ◽  
Tissue Repair ◽  
Matrix Composites ◽  
Soft Tissue Repair ◽  
Decellularized Extracellular Matrix ◽  
Biological Functionality ◽  
Traditional Approaches

Decellularized extracellular matrix (dECM)–based biomaterials are of great clinical utility in soft tissue repair applications due to their regenerative properties. Multi-layered dECM devices have been developed for clinical indications where additional thickness and biomechanical performance are required. However, traditional approaches to the fabrication of multi-layered dECM devices introduce additional laminating materials or chemical modifications of the dECM that may impair the biological functionality of the material. Using an established dECM biomaterial, ovine forestomach matrix, a novel method for the fabrication of multi-layered dECM constructs has been developed, where layers are bonded via a physical interlocking process without the need for additional bonding materials or detrimental chemical modification of the dECM. The versatility of the interlocking process has been demonstrated by incorporating a layer of hyaluronic acid to create a composite material with additional biological functionality. Interlocked composite devices including hyaluronic acid showed improved in vitro bioactivity and moisture retention properties.

scholarly journals The Use of a Green Fluorescent Protein Porcine Model to Evaluate Host Tissue Integration into Extracellular Matrix Derived Bionanocomposite Scaffolds

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
S. E. Smith ◽  
R. A. White ◽  
D. A. Grant ◽  
S. A. Grant
Keyword(s):  
Extracellular Matrix ◽  
Green Fluorescent Protein ◽  
Soft Tissue ◽  
Tissue Repair ◽  
Fluorescent Protein ◽  
Host Tissue ◽  
Soft Tissue Repair ◽  
Tissue Integration ◽  
Tissue Migration ◽  
Green Fluorescent

When using heterogeneous extracellular matrix (ECM) derived scaffolds for soft tissue repair, current methods of in vivo evaluation can fail to provide a clear distinction between host collagen and implanted scaffolds making it difficult to assess host tissue integration and remodeling. The purpose of this study is both to evaluate novel scaffolds conjugated with nanoparticles for host tissue integration and biocompatibility and to assess green fluorescent protein (GFP) expressing swine as a new animal model to evaluate soft tissue repair materials. Human-derived graft materials conjugated with nanoparticles were subcutaneously implanted into GFP expressing swine to be evaluated for biocompatibility and tissue integration through histological scoring and confocal imaging. Histological scoring indicates biocompatibility and remodeling of the scaffolds with and without nanoparticles at 1, 3, and 6 months. Confocal microscope images display host tissue integration into scaffolds although nonspecificity of GFP does not allow for quantification of integration. However, the confocal images do allow for spatial observation of host tissue migration into the scaffolds at different depths of penetration. The study concludes that the nanoparticle scaffolds are biocompatible and promote integration and that the use of GFP expressing swine can aid in visualizing the scaffold/host interface and host cell/tissue migration.

Extracellular Matrix Biomaterials for Soft Tissue Repair

2009 ◽  
Vol 26 (4) ◽  
pp. 507-523 ◽  
Author(s):  
Kevin G. Cornwell ◽  
Adam Landsman ◽  
Kenneth S. James
Keyword(s):  
Extracellular Matrix ◽  
Soft Tissue ◽  
Tissue Repair ◽  
Soft Tissue Repair

scholarly journals A facile, versatile hydrogel bioink for 3D bioprinting benefits long-term subaqueous fidelity, cell viability and proliferation

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Hongqing Chen ◽  
Fei Fei ◽  
Xinda Li ◽  
Zhenguo Nie ◽  
Dezhi Zhou ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Cell Viability ◽  
Tissue Repair ◽  
Three Dimensional ◽  
Water Retention Capacity ◽  
3D Bioprinting ◽  
Soft Tissue Repair ◽  
Retention Capacity

Abstract Both of the long-term fidelity and cell viability of three-dimensional (3D)-bioprinted constructs are essential to precise soft tissue repair. However, the shrinking/swelling behavior of hydrogels brings about inadequate long-term fidelity of constructs, and bioinks containing excessive polymer are detrimental to cell viability. Here, we obtained a facile hydrogel by introducing 1% aldehyde hyaluronic acid (AHA) and 0.375% N-carboxymethyl chitosan (CMC), two polysaccharides with strong water absorption and water retention capacity, into classic gelatin (GEL, 5%)–alginate (ALG, 1%) ink. This GEL–ALG/CMC/AHA bioink possesses weak temperature dependence due to the Schiff base linkage of CMC/AHA and electrostatic interaction of CMC/ALG. We fabricated integrated constructs through traditional printing at room temperature and in vivo simulation printing at 37°C. The printed cell-laden constructs can maintain subaqueous fidelity for 30 days after being reinforced by 3% calcium chloride for only 20 s. Flow cytometry results showed that the cell viability was 91.38 ± 1.55% on day 29, and the cells in the proliferation plateau at this time still maintained their dynamic renewal with a DNA replication rate of 6.06 ± 1.24%. This work provides a convenient and practical bioink option for 3D bioprinting in precise soft tissue repair.

scholarly journals Dynamics of hip joint effusion after posterior soft tissue repair in total hip arthroplasty

2006 ◽  
Vol 30 (4) ◽  
pp. 233-236 ◽  
Author(s):  
Sarunas Tarasevicius ◽  
Uldis Kesteris ◽  
Romas Jonas Kalesinskas ◽  
Hans Wingstrand
Keyword(s):  
Soft Tissue ◽  
Total Hip Arthroplasty ◽  
Hip Joint ◽  
Hip Arthroplasty ◽  
Tissue Repair ◽  
Joint Effusion ◽  
Soft Tissue Repair ◽  
Total Hip ◽  
Posterior Soft Tissue
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