scholarly journals A facile, versatile hydrogel bioink for 3D bioprinting benefits long-term subaqueous fidelity, cell viability and proliferation

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Hongqing Chen ◽  
Fei Fei ◽  
Xinda Li ◽  
Zhenguo Nie ◽  
Dezhi Zhou ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Cell Viability ◽  
Tissue Repair ◽  
Three Dimensional ◽  
Water Retention Capacity ◽  
3D Bioprinting ◽  
Soft Tissue Repair ◽  
Retention Capacity

Abstract Both of the long-term fidelity and cell viability of three-dimensional (3D)-bioprinted constructs are essential to precise soft tissue repair. However, the shrinking/swelling behavior of hydrogels brings about inadequate long-term fidelity of constructs, and bioinks containing excessive polymer are detrimental to cell viability. Here, we obtained a facile hydrogel by introducing 1% aldehyde hyaluronic acid (AHA) and 0.375% N-carboxymethyl chitosan (CMC), two polysaccharides with strong water absorption and water retention capacity, into classic gelatin (GEL, 5%)–alginate (ALG, 1%) ink. This GEL–ALG/CMC/AHA bioink possesses weak temperature dependence due to the Schiff base linkage of CMC/AHA and electrostatic interaction of CMC/ALG. We fabricated integrated constructs through traditional printing at room temperature and in vivo simulation printing at 37°C. The printed cell-laden constructs can maintain subaqueous fidelity for 30 days after being reinforced by 3% calcium chloride for only 20 s. Flow cytometry results showed that the cell viability was 91.38 ± 1.55% on day 29, and the cells in the proliferation plateau at this time still maintained their dynamic renewal with a DNA replication rate of 6.06 ± 1.24%. This work provides a convenient and practical bioink option for 3D bioprinting in precise soft tissue repair.

scholarly journals The Application of Three-Dimensional Collagen-Scaffolds Seeded with Myoblasts to Repair Skeletal Muscle Defects

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Jianqun Ma ◽  
Kyle Holden ◽  
Jinhong Zhu ◽  
Haiying Pan ◽  
Yong Li
Keyword(s):  
Soft Tissue ◽  
Muscle Tissue ◽  
Tissue Repair ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Tissue Repair And Regeneration ◽  
Tissue Constructs ◽  
Defect Sites

Three-dimensional (3D) engineered tissue constructs are a novel and promising approach to tissue repair and regeneration. 3D tissue constructs have the ability to restore form and function to damaged soft tissue unlike previous methods, such as plastic surgery, which are able to restore only form, leaving the function of the soft tissue often compromised. In this study, we seeded murine myoblasts (C2C12) into a collagen composite scaffold and cultured the scaffold in a roller bottle cell culture system in order to create a 3D tissue graftin vitro. The 3D graft createdin vitrowas then utilized to investigate muscle tissue repairin vivo. The 3D muscle grafts were implanted into defect sites created in the skeletal muscles in mice. We detected that the scaffolds degraded slowly over time, and muscle healing was improved which was shown by an increased quantity of innervated and vascularized regenerated muscle fibers. Our results suggest that the collagen composite scaffold seeded with myoblasts can create a 3D muscle graftin vitrothat can be employed for defect muscle tissue repairin vivo.

scholarly journals Artificial Tumor Microenvironments in Neuroblastoma

Cancers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1629
Author(s):  
Colin H. Quinn ◽  
Andee M. Beierle ◽  
Elizabeth A. Beierle
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Therapeutic Interventions ◽  
3D Bioprinting ◽  
Culture Studies ◽  
In Vivo Models ◽  
Novel Treatments ◽  
Tumor Microenvironments ◽  
Novel Approach

In the quest to advance neuroblastoma therapeutics, there is a need to have a deeper understanding of the tumor microenvironment (TME). From extracellular matrix proteins to tumor associated macrophages, the TME is a robust and diverse network functioning in symbiosis with the solid tumor. Herein, we review the major components of the TME including the extracellular matrix, cytokines, immune cells, and vasculature that support a more aggressive neuroblastoma phenotype and encumber current therapeutic interventions. Contemporary treatments for neuroblastoma are the result of traditional two-dimensional culture studies and in vivo models that have been translated to clinical trials. These pre-clinical studies are costly, time consuming, and neglect the study of cofounding factors such as the contributions of the TME. Three-dimensional (3D) bioprinting has become a novel approach to studying adult cancers and is just now incorporating portions of the TME and advancing to study pediatric solid. We review the methods of 3D bioprinting, how researchers have included TME pieces into the prints, and highlight present studies using neuroblastoma. Ultimately, incorporating the elements of the TME that affect neuroblastoma responses to therapy will improve the development of innovative and novel treatments. The use of 3D bioprinting to achieve this aim will prove useful in developing optimal therapies for children with neuroblastoma.

scholarly journals Dynamics of hip joint effusion after posterior soft tissue repair in total hip arthroplasty

2006 ◽  
Vol 30 (4) ◽  
pp. 233-236 ◽  
Author(s):  
Sarunas Tarasevicius ◽  
Uldis Kesteris ◽  
Romas Jonas Kalesinskas ◽  
Hans Wingstrand
Keyword(s):  
Soft Tissue ◽  
Total Hip Arthroplasty ◽  
Hip Joint ◽  
Hip Arthroplasty ◽  
Tissue Repair ◽  
Joint Effusion ◽  
Soft Tissue Repair ◽  
Total Hip ◽  
Posterior Soft Tissue
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