scholarly journals The Arakawa Reaction and the Inorganic Sulphate Content of Human Milk

1937 ◽  
Vol 31 (3-4) ◽  
pp. 287-305 ◽  
Author(s):  
Kyohachi Yoshino
1931 ◽  
Vol 25 (4) ◽  
pp. 1237-1243 ◽  
Author(s):  
David Paton Cuthbertson ◽  
Sidney Lionel Tompsett

Glycosaminoglycan turn-over has been studied both in vivo and in vitro , by using sodium [ 35 S]sulphate as a precursor. The in vivo experiments were performed on rabbits and dogs, taking special care to monitor the 35 S radioactivity in the serum throughout the experiment and to measure the radioactivity due to unincorporated inorganic [ 35 S] sulphate in cartilage at the end of each experiment, in addition to that due to incorporated sulphate. The inorganic sulphate content of the serum was also determined as well as the distribution coefficient for the inorganic sulphate ion between cartilage and serum. From this information it was possible to calculate accurately the rate of sulphate uptake by cartilage in vivo and hence the turn-over rate. Experiments were then performed in vitro on cartilage from rabbits and dogs and the in vivo and in vitro results were compared. A very good agreement was obtained between the two sets of results. Studies were then carried out under exactly the same in vitro conditions on human articular cartilage and it was thus possible to obtain a turn-over rate for the latter which one could trust was close to the actual in vivo value. The mean half-lives thus obtained varied from 45 days for the young rabbit to 150 days for the adult dog and 800 days for the human femoral head. In human cartilage there were considerable variations in turn-over rate within a single joint as a function of depth below the surface, and between different joints. Thus, while the mean half-life for the human femoral head is 800 days, that for the femoral condyle is 300 days. Cartilage from osteoarthrosic femoral heads did not appear to differ much with respect to sulphate uptake from the normal specimens although the turn-over rates were somewhat higher.


1958 ◽  
Vol 9 (3) ◽  
pp. 373 ◽  
Author(s):  
PJ Mylrea

The influence of molybdenum, inorganic sulphate, and manganese upon the copper status of cattle was investigated. The mean liver copper concentration increased for steers on a basal diet containing 7.6 p.p.m. copper, 2.4 p.p.m. molybdenum, 91 p.p.m. manganese, and 0.03 per cent. sulphate. In steers on a similar diet but with the sulphate content increased to 0.55 per cent., with or without an increase in the manganese content to 391 p.p.m., there was a highly significant reduction (P=0.01) in the liver copper concentrations but not to low levels. There was also a significant (P=0.01) increase in the serum inorganic sulphate levels. On increasing the molybdenum content to 9.2 p.p.m. there was a further reduction, to low levels, in the liver and blood copper concentrations of steers on the high sulphate diets but there was no definite effect on those on the low sulphate diet. Manganese again appeared to be without effect. It was concluded that there was a molybdenum-sulphate interaction with copper in cattle but that, under the conditions of this trial, manganese was without effect. Despite the very low liver and blood copper levels attained, and maintained for 15 weeks, there was no clinical evidence of hypocuprosis. The mineral and sulphate levels applied in some of these treatments are similar to those found in pastures from areas in New South Wales where copper deficiency in cattle occurs. The levels in pastures, as related to the results of this trial, are discussed.


2019 ◽  
Vol 62 (6) ◽  
pp. 605-613 ◽  
Author(s):  
Leonilo F. Endoma ◽  
Loda M. Nacional ◽  
Rovilla J. Luhan

Abstract The current industrial practice of using chemical bleach to achieve the pure white colour of agar is deleterious to both human and environmental health. This study evaluates the potential of solar irradiation as an alternative bleaching process for agar extracted from Gracilariopsis heteroclada in Iloilo, Philippines. The physico-chemical properties of agar obtained from alkaline-treated seaweed after exposure to different bleaching conditions (e.g. solar irradiation, hypochlorite solution, and ultraviolet and fluorescent lights) were examined and compared with commercial bacteriological agar. Photobleaching through solar irradiation produced agar with superior gel strength (1038.61 g cm−2), high 3,6-anhydrogalactose content (41.44%) and low total inorganic sulphate content (1.87%) without compromising agar yield (19.37%). Solar irradiation offers very promising results as a simple, low-cost, environmentally friendly alternative to the chlorine bleaching process for agar extraction.


1956 ◽  
Vol 7 (1) ◽  
pp. 45 ◽  
Author(s):  
KN Wynne ◽  
GL McClymont

A basal diet containing 6.2 p.p.m. copper, 0.8 p.p.m. molybdenum, and 0.04 per cent. inorganic sulphate was fed unsupplenlented to one group of sheep. Four other groups were fed on the same diet supplemented with ammonium molybdate to give a molybdenum content of 5.1 p.p.m. or with sodium sulphate to give an inorganic sulphate content of 0.40 per cent., or with both. Sheep on the unsupplemented basal diet showed no marked disturbance of their liver and blood copper concentrations. Sheep maintained for 50 weeks on the diet containing both molybdenum and sulphate supplements showed a progressive fall in liver and blood copper levels, and developed dystrophic wool and hypochromatrichia. On a restricted feed intake of the same diet, sheep displayed a similar fall in liver copper and similar wool changes, but maintained a normal blood copper level for a longer period before developing hypocupraemia. Hypochromatrichia appeared to develop in this group before hypocupraemia was evident. With only the sulphate supplement added to the basal diet, liver copper concentrations fell, but not to a level associated with hypocupraemia. With only the molybdenum supplement added to the basal diet, copper retention by the liver was depressed to a small, but significant extent compared with the controls. The diet of the groups showing evidence of hypocuprosis approximately reproduced the mean copper, molybdenum, and sulphate status of the feed in an area in New South Wales enzootically affected with hypocuprosis. It is concluded that hypocuprosis in this area at least is explicable in terms of copper-molybdenum-sulphate interaction.


1954 ◽  
Vol 5 (3) ◽  
pp. 511 ◽  
Author(s):  
AT Dick

Experiments reported in this paper show that increased copper intake of sheep is reflected by increased copper content of the liver. The magnitude of the rise in liver copper depends upon a number of factors, but under the described conditions, the amount of copper accumulated by the animal is proportional to the copper intake within the range 3-20 mg per day; the amount stored in the liver over a 6 months1 period was found to be 4.5-5 per cent. of the intake. Some of the conditions which may modify the retention of copper were examined. It was found that the addition of ferrous sulphide to the diet, which presumably converts added copper to the insoluble sulphide, lowered the expected copper accumulation in the liver by 75 per cent. Conditions which may modify this effect of ferrous sulphide have been examined and described. Other possible sources of sulphur for the formation of copper sulphide in the animal (elemental sulphur, sodium thiosulphate) were found not to be effective. The effects of some other metals (zinc, nickel, iron, and molybdenum) on copper accumulation in the liver were examined. Molybdenum was found to have a severely limiting effect, but this effect was only observed when the diet also contained a sufficient quantity of inorganic sulphate. For a given intake of molybdenum the limitation of copper storage was found to be proportional to the sulphate content of the diet. The possible bearing of these experimental findings on conflicting field observations relating to copper metabolism of sheep is discussed.


1980 ◽  
Vol 44 (1) ◽  
pp. 7-12 ◽  
Author(s):  
E. Leerbeck ◽  
H. Søndergaard

1. It was shown that the water-soluble fraction of cow's milk and human milk did no possess significant antirachitic activity on rats.2. Whole cow's milk was found to contain 38 i.u. vitamin D/1. Whole human milk contained 15 i.u. vitamin D/1, of which 12 i.u. derived from the lipid fraction.3. Thus the values found were much lower than the 204 i.u./1 in cow's milk and 950 i.u./1 in human milk given in the literature based on chemical determination of the sterol sulphate content.


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