scholarly journals Studies on the Nutrition of the Rice Plant with Special Reference to Nitrogen and Silica. : I. Translocation of absorbed silica in plant and its accumulation in the epidermal cells of leaf blade in relation to transpiration.

1955 ◽  
Vol 24 (1) ◽  
pp. 29-33 ◽  
Author(s):  
Isamu BABA
Keyword(s):  
Special Reference ◽  
Rice Plant ◽  
Leaf Blade ◽  
Epidermal Cells

Effects of Varying Salinity on Leaf Ultrastructure of Zannichellia Palustris

2001 ◽  
Vol 7 (S2) ◽  
pp. 62-63
Author(s):  
A.D. Barnabas ◽  
R. Jimmy ◽  
K. Govender ◽  
W.J. Przybylowicz ◽  
J. Mesjasz-Przybylowicz
Keyword(s):  
Leaf Blade ◽  
High Salinity ◽  
Imaging System ◽  
Major Elements ◽  
Epidermal Cells ◽  
Nuclear Microprobe ◽  
Elemental Distribution ◽  
Leaf Ultrastructure ◽  
Distribution Maps ◽  
Freeze Dried

Zanichellia palustris is a submerged halophyte which occurs in waters of low salinity (5%o to 10%o). It is not capable of surviving at high salinity. Reasons for its inability to tolerate hypersaline conditions is important to our understanding of its biology. in the present study, leaf ultrastructure of plants growing at normal salinity was compared with plants growing at high salinity so as to assess the effects of hypersalinity. Attention was focused on ultrstructural changes occurring in leaf blade epidermal cells.Plants were grown in seawater at two salinities : 10%o (control) and 20%o (high salinity). Pieces of mature leaf blades from both treatments were harvested and prepared for Transmission Electron Microscopy (TEM) following standard procedures. in addition, the overall distribution and concentration of chlorine (Cl) in the leaves was ascertained since it is the most abundant anion in seawater and is important in considerations of salt tolerance in submerged halophytes. Cl was determined by means of a nuclear microprobe. Pieces of sectioned leaves were rapidly frozen, freeze dried and carbon coated. Elemental distribution maps of Cl were obtained using the true elemental imaging system (Dynamic Analysis). Two complementary techniques, Proton Induced X-Ray Emission (PLXE) and Proton Back Scattering (BS) were performed simultaneously using the GeoPIXE suite of programmes. Sample thickness and composition of major elements were obtained from the BS spectra.The ultrastructural morphology of leaf blade epidermal cells, which possess transfer cell characteristics differed at each salinity. While the cytoplasm was well preserved in epidermal cells of plants at the control salinity : 10%o (Fig 1), considerable cytoplasmic damage occurred in epidermal cells of plants at high salinity, in the form of breakdown of membranes such as the plasmamembrane and tonoplast, and senescence of various organelles including chloroplasts and mitochondria (Fig. 2).

scholarly journals Early detection of injuries in leaves of Clusia hilariana Schltdl. (Clusiaceae) caused by particulate deposition of iron

2014 ◽  
Vol 38 (3) ◽  
pp. 423-432 ◽  
Author(s):  
Diego Ismael Rocha ◽  
Luzimar Campos da Silva ◽  
Eduardo Gusmão Pereira ◽  
Bruno Francisco Sant'Anna-Santos ◽  
Elisa Rodrigues Gontijo ◽  
...  
Keyword(s):  
Leaf Blade ◽  
Epidermal Cells ◽  
Anticlinal Wall ◽  
Abaxial Surface ◽  
Particulate Deposition ◽  
Particulate Iron ◽  
Young Leaves ◽  
Leaf Tissues ◽  
Clusia Hilariana ◽  
Microscopy Techniques

This study aims to evaluate the prognostic value of microscopic parameters of asymptomatic leaves of Clusia hilariana Schltdl. subjected to particulate deposition of iron (2.14 mg cm-2 day-1) for 45 consecutive days. Samples of young and expanded leaves without symptoms were collected and subjected to light and scanning electron microscopy techniques. The height of the epidermal cells on both surfaces of the leaf and the thickness of the hypodermis, the chlorophyll parenchyma, and the leaf blade were measured. Micromorphological injury occurred in the abaxial surface of young leaves and on both surfaces of expanded leaves. Erosion of the epicuticular wax and cuticle rupture were frequent on the adaxial surface, while on the abaxial surface of both leaves there was a loss of sinuosity on the anticlinal wall of the epidermal cells, stomatal deformity and obstruction. Micromorphometric alterations were seen in all leaf tissues except in the height of epidermic cells, probably due to the thick cuticle and prominent cuticular flanges. The highest difference in thickness of the leaf blade was seen in young leaves of plants subjected to SPMFe, indicating greater sensibility to particulate iron in comparison to the expanded leaves. The micromorphological and micromorphometric alterations in the leaf blade of Clusia hilariana Schltdl. showed the prognostic potential of these tools on the evaluation of impacts caused by the deposition of particulate matter, especially in the 'Restinga' natural vegetation, where the exposure is increasing due to the presence of iron ore industry in their surroundings.

scholarly journals Anatomical Study of Some Species of the Brassicaceae Family

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Ahmed Hassan Kadhim ◽  
Ahmed Abies Moter
Keyword(s):  
Leaf Blade ◽  
Anatomical Study ◽  
Epidermal Cells ◽  
Science Department ◽  
Lower Epidermis ◽  
Complex Cells ◽  
Brassicaceae Family

Study complete in postgraduate laboratories at the College of Science, Department of Biology, University of Kufa. The anatomical study revealed a variation in the formation of complex cells and epidermal cells, where they differed in their shapes and were irregular with wavy ridges and on the axial surfaces in R. sativusvar red L. and R. sativuslongipinntus L. Their walls were weak jagged in only R. raphenstrum. Also, the stomata were located on both sides of the leaf blade, and the upper epidermis contained fewer stomata compared to the lower epidermis.

Effects of Varying Salinity on the Endoplasmic Reticulum System in Leaf Epidermal Cells of Ruppia Maritima

1997 ◽  
Vol 3 (S2) ◽  
pp. 97-98
Author(s):  
A.D. Barnabas
Keyword(s):  
Endoplasmic Reticulum ◽  
Leaf Blade ◽  
Membrane System ◽  
Epidermal Cells ◽  
Ruppia Maritima ◽  
Selective Staining ◽  
Transmission Electron ◽  
Wide Range ◽  
Aqueous Mixture ◽  
Conventional Manner

Ruppia maritima L. is a submerged halophyte which is capable of growing in a wide range of salinities. In a previous study, the effects of varying salinity on the ultrastructure of leaf blade epidermal cells of R. maritima were investigated. The ultrastructural morphology of the cells, including organelle number and distribution, differed at different salinities. However, salinity effects on the endoplasmic reticulum (ER) system could not be assessed since this cytoplasmic component was not readily discernible in material processed in the conventional manner for transmission electron microscopy (TEM). In the present study, selective staining of the ER was achieved, and the response of this membrane system to different salinities ascertained.Leaf material from plants growing at two salinities (5‰and 20‰) was examined. The zinc iodide-osmium tetroxide (ZIO) impregnation technique was used to stain the ER, and its morphology and distribution determined in leaf blade epidermal cells exposed to the different salinities. Leaf samples were fixed in glutaraldehyde, post fixed in an aqueous mixture of ZIO, dehydrated, and embedded in resin. Controls were processed in exactly the same way, but without ZIO post fixation. Sections with dark gold to purple interference colours were cut and examined unstained with a Phillips 301 TEM.

Studies on Growth of Leaves of Paddy Rice Plant. : I. The Growth of the Leaf-blade and Leaf-Sheath.

1954 ◽  
Vol 23 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Kaichi ARASHII ◽  
Hiroshi EGUCHI
Keyword(s):  
Rice Plant ◽  
Leaf Blade ◽  
Leaf Sheath ◽  
Paddy Rice
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