scholarly journals Varietal Difference in Pre-germination Flooding Tolerance and Waterlogging Tolerance at the Seedling Stage in Maize Inbred Lines.

2002 ◽  
Vol 71 (3) ◽  
pp. 361-367 ◽  
Author(s):  
Yoshiro MANO ◽  
Masanori MURAKI ◽  
Toshinori KOMATSU ◽  
Masahiro FUJIMORI ◽  
Fumiaki AKIYAMA ◽  
...  
2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Zhiming Zhang ◽  
Feng Jin ◽  
Cui Wang ◽  
Jun Luo ◽  
Haijian Lin ◽  
...  

In the last two decades, the accumulation of heavy metal in crop grains has become the study hotspot. In this study, 19 representative elite maize inbred lines and 3 hybrid varieties were investigated at the seedling stage, which can accumulate Pb and Cd in the stems and leaves, respectively. The results demonstrated that significant differences are among inbred lines for accumulation of heavy metals, implying that the Cd accumulation is significant correlation between the male parents and their hybrids and some inbred lines have been selected for cross-breeding with low Pb or Cd accumulation, such as S37, 9782, and ES40; Moreover, some inbred lines could be suitable for phytoremediation species for soil bioremediation with high levels of Pb and Cd accumulation, including 178, R08, 48-2, and Mo17ht.


2014 ◽  
Vol 40 (5) ◽  
pp. 838 ◽  
Author(s):  
Chao CUI ◽  
Ju-Lin GAO ◽  
Xiao-Fang YU ◽  
Zhi-Jun SU ◽  
Zhi-Gang WANG ◽  
...  

2013 ◽  
Vol 39 (12) ◽  
pp. 2253
Author(s):  
Qing-Jiu YAN ◽  
Shi-Ping HUO ◽  
Fang-Kui ZHANG ◽  
Xing-Duan ZHANG ◽  
Jian ZHANG ◽  
...  

2017 ◽  
Vol 43 (9) ◽  
pp. 1328 ◽  
Author(s):  
Bo-Xin WANG ◽  
Ya-Hui WANG ◽  
Peng-Fei CHEN ◽  
Xu-Dong-Yu LIU ◽  
Zhi-Qian FENG ◽  
...  

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhengjie Chen ◽  
Dengguo Tang ◽  
Jixing Ni ◽  
Peng Li ◽  
Le Wang ◽  
...  

Abstract Background Maize is one of the most important field crops in the world. Most of the key agronomic traits, including yield traits and plant architecture traits, are quantitative. Fine mapping of genes/ quantitative trait loci (QTL) influencing a key trait is essential for marker-assisted selection (MAS) in maize breeding. However, the SNP markers with high density and high polymorphism are lacking, especially kompetitive allele specific PCR (KASP) SNP markers that can be used for automatic genotyping. To date, a large volume of sequencing data has been produced by the next generation sequencing technology, which provides a good pool of SNP loci for development of SNP markers. In this study, we carried out a multi-step screening method to identify kompetitive allele specific PCR (KASP) SNP markers based on the RNA-Seq data sets of 368 maize inbred lines. Results A total of 2,948,985 SNPs were identified in the high-throughput RNA-Seq data sets with the average density of 1.4 SNP/kb. Of these, 71,311 KASP SNP markers (the average density of 34 KASP SNP/Mb) were developed based on the strict criteria: unique genomic region, bi-allelic, polymorphism information content (PIC) value ≥0.4, and conserved primer sequences, and were mapped on 16,161 genes. These 16,161 genes were annotated to 52 gene ontology (GO) terms, including most of primary and secondary metabolic pathways. Subsequently, the 50 KASP SNP markers with the PIC values ranging from 0.14 to 0.5 in 368 RNA-Seq data sets and with polymorphism between the maize inbred lines 1212 and B73 in in silico analysis were selected to experimentally validate the accuracy and polymorphism of SNPs, resulted in 46 SNPs (92.00%) showed polymorphism between the maize inbred lines 1212 and B73. Moreover, these 46 polymorphic SNPs were utilized to genotype the other 20 maize inbred lines, with all 46 SNPs showing polymorphism in the 20 maize inbred lines, and the PIC value of each SNP was 0.11 to 0.50 with an average of 0.35. The results suggested that the KASP SNP markers developed in this study were accurate and polymorphic. Conclusions These high-density polymorphic KASP SNP markers will be a valuable resource for map-based cloning of QTL/genes and marker-assisted selection in maize. Furthermore, the method used to develop SNP markers in maize can also be applied in other species.


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