scholarly journals Gene expression profiling identifies liver X receptor alpha as an estrogen-regulated gene in mouse adipose tissue

2004 ◽  
Vol 32 (3) ◽  
pp. 879-892 ◽  
Author(s):  
L Lundholm ◽  
S Moverare ◽  
KR Steffensen ◽  
M Nilsson ◽  
M Otsuki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profiling ◽  
White Adipose Tissue ◽  
Expression Profiling ◽  
Liver X Receptor ◽  
Atp Binding ◽  
Mrna Levels ◽  
Ovariectomized Mice ◽  
Atp Binding Cassette

Estrogens reduce adipose tissue mass in both humans and animals. The molecular mechanisms for this effect are, however, not well characterized. We took a gene expression profiling approach to study the direct effects of estrogen on mouse white adipose tissue (WAT). Female ovariectomized mice were treated for 10, 24 and 48 h with 17beta-estradiol or vehicle. RNA was extracted from gonadal fat and hybridized to Affymetrix MG-U74Av2 arrays. 17beta-Estradiol was shown to decrease mRNA expression of liver X receptor (LXR) alpha after 10 h of treatment compared with the vehicle control. The expression of several LXRalpha target genes, such as sterol regulatory element-binding protein 1c, apolipoprotein E, phospholipid transfer protein, ATP-binding cassette A1 and ATP-binding cassette G1, was similarly decreased. We furthermore identified a 1.5 kb LXRalpha promoter fragment that is negatively regulated by estrogen. Several genes involved in lipogenesis and lipolysis were identified as novel targets that could mediate estrogenic effects on adipose tissue. Finally, we show that ERalpha is the main estrogen receptor expressed in mouse white adipose tissue (WAT) with mRNA levels several hundred times higher than those of ERbeta mRNA.

scholarly journals Gene Expression Profiling of Transporters in the Solute Carrier and ATP-Binding Cassette Superfamilies in Human Eye Substructures

2013 ◽  
Vol 10 (2) ◽  
pp. 650-663 ◽  
Author(s):  
Amber Dahlin ◽  
Ethan Geier ◽  
Sophie L. Stocker ◽  
Cheryl D. Cropp ◽  
Elena Grigorenko ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Atp Binding ◽  
Human Eye ◽  
Solute Carrier ◽  
Atp Binding Cassette

scholarly journals Gene expression profiling of white adipose tissue reveals paternal transmission of proneness to obesity

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Sumiyo Morita ◽  
Kazuhiko Nakabayashi ◽  
Tomoko Kawai ◽  
Keiko Hayashi ◽  
Takuro Horii ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profiling ◽  
White Adipose Tissue ◽  
Expression Profiling ◽  
Paternal Transmission

scholarly journals Gene Expression Profiling Reveals Defined Functions of the ATP-Binding Cassette Transporter COMATOSE Late in Phase II of Germination

2007 ◽  
Vol 143 (4) ◽  
pp. 1669-1679 ◽  
Author(s):  
Esther Carrera ◽  
Tara Holman ◽  
Anne Medhurst ◽  
Wendy Peer ◽  
Heike Schmuths ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Phase Ii ◽  
Expression Profiling ◽  
Atp Binding ◽  
Atp Binding Cassette Transporter ◽  
Atp Binding Cassette

scholarly journals Gestational age-dependent gene expression profiling of ATP-binding cassette transporters in the healthy human placenta

2018 ◽  
Vol 23 (1) ◽  
pp. 610-618 ◽  
Author(s):  
Guinever E. Imperio ◽  
Mohsen Javam ◽  
Phetcharawan Lye ◽  
Andrea Constantinof ◽  
Caroline E. Dunk ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Gestational Age ◽  
Human Placenta ◽  
Expression Profiling ◽  
Atp Binding ◽  
Healthy Human ◽  
Atp Binding Cassette Transporters ◽  
Age Dependent ◽  
Atp Binding Cassette

Metallothionein gene expression and secretion in white adipose tissue

2000 ◽  
Vol 279 (6) ◽  
pp. R2329-R2335 ◽  
Author(s):  
Paul Trayhurn ◽  
Jacqueline S. Duncan ◽  
Anne M. Wood ◽  
John H. Beattie
Keyword(s):  
Gene Expression ◽  
Molecular Weight ◽  
Adipose Tissue ◽  
White Adipose Tissue ◽  
Stromal Vascular Fraction ◽  
Low Molecular Weight ◽  
Secretory Product ◽  
Mrna Levels ◽  
Gene Encoding ◽  
Adrenoceptor Agonist

White adipose tissue (WAT) has been examined to determine whether the gene encoding metallothionein (MT), a low-molecular-weight stress response protein, is expressed in the tissue and whether MT may be a secretory product of adipocytes. The MT-1 gene was expressed in epididymal WAT, with MT-1 mRNA levels being similar in lean and obese ( ob/ ob) mice. MT-1 mRNA was found in each of the main adipose tissue sites (epididymal, perirenal, omental, subcutaneous), and there was no major difference between depots. Separation of adipocytes from the stromal-vascular fraction of WAT indicated that the MT gene (MT-1 and MT-2) was expressed in adipocytes themselves. Treatment of mice with zinc had no effect on MT-1 mRNA levels in WAT, despite strong induction of MT-1 expression in the liver. MT-1 gene expression in WAT was also unaltered by fasting or norepinephrine. However, administration of a β3-adrenoceptor agonist, BRL-35153A, led to a significant increase in MT-1 mRNA. On differentiation of fibroblastic preadipocytes to adipocytes in primary culture, MT was detected in the medium, suggesting that the protein may be secreted from WAT. It is concluded that WAT may be a significant site of MT production; within adipocytes, MT could play an antioxidant role in protecting fatty acids from damage.

Gene expression profiling of gluteal adipose tissue after prolonged bedrest

2018 ◽  
Vol 275 ◽  
pp. e77
Author(s):  
R. Colonna ◽  
E. Dalla Nora ◽  
J.M. Sanz ◽  
M.A. Miselli ◽  
M.L. Morieri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Gene Expression Profiling ◽  
Expression Profiling
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