THE UPTAKE AND AUTORADIOGRAPHIC LOCALIZATION OF TRITIATED OESTRADIOL IN THE RAT UTERUS AFTER LOCAL APPLICATION

1968 ◽  
Vol 41 (1) ◽  
pp. 69-NP ◽  
Author(s):  
BETTY G. MOBBS
Keyword(s):  
Local Application ◽  
Ovariectomized Rats ◽  
Uterine Tissue ◽  
Rat Uterus ◽  
Free Steroid

SUMMARY The distribution of radioactivity present in uterine tissue was investigated at various times after the injection of small amounts of tritiated oestradiol into the lumen of the uteri of ovariectomized rats. The highest amounts of radioactivity were present as free steroid 30 min. to 1 hr. after injection and a considerable amount was still present after 5 hr. Autoradiographs indicated that the distribution of labelling changed with time after injection, being heaviest initially in the epithelium, but being mainly located in or near the nuclei of both epithelium and stroma when maximum radioactivity was present. It is suggested that these sites are specific for oestrogens, and that many of the sites initially occupied are unspecific.

METABOLISM OF 20β-HYDROXY-4-PREGNEN-3-ONE IN UTERINE TISSUE OF NON-PREGNANT RATS IN VITRO

1976 ◽  
Vol 83 (3) ◽  
pp. 604-620 ◽  
Author(s):  
B. P. Lisboa ◽  
M. Holtermann
Keyword(s):  
Biological Activity ◽  
Adult Female ◽  
Female Rats ◽  
Uterine Tissue ◽  
Rat Uterus ◽  
Adult Rats ◽  
Pregnant Rats ◽  
Progesterone Metabolites ◽  
Ovariectomized Adult Rats

ABSTRACT In vitro experiments carried out with uterus preparations of ovariectomized adult rats indicate the presence in this tissue of a 20β-hydroxysteroid-oxidoreductase which catalyzes the conversion of 20β-hydroxy-4-pregnen-3-one to progesterone. Since a hepatic 20β-hydroxysteroid-oxidoreductase is absent in adult female rats, the myometrial enzyme can be responsible for the biological activity of 20β-hydroxy-4-pregnen-3-one in these animals. Besides progesterone five metabolites were isolated and identified after incubation of [4-14C]20β-hydroxy-4-pregnen-3-one with uterine tissue: 20β-hydroxy-5α-pregnan-3-one, 20β-hydroxy-5β-pregnan-3-one, 5α-pregnane-3α,20β-diol, 4-pregnene-3α,20β-diol and 4-pregnene-3β,20β-diol. The conversion of 20β-hydroxy-4-pregnen-3-one to progesterone permits us to regard all five steroids isolated as progesterone metabolites in the rat uterus. 20β-hydroxy-5β-pregnan-3-one is the first C21-metabolite with a 5β(H)-configuration isolated in the rat uterus, which indicates the presence of 5β-reductase in this tissue.

Metabolism of [3H]noradrenaline by the isolated rat uterus

1981 ◽  
Vol 59 (12) ◽  
pp. 1245-1249 ◽  
Author(s):  
Julio Fernández-Pardal ◽  
Martha F. Gimeno ◽  
Alvaro L. Gimeno
Keyword(s):  
Estrogen Treatment ◽  
Ovariectomized Rats ◽  
Noradrenaline Metabolism ◽  
Rat Uterus ◽  
Metabolic Pattern ◽  
Isolated Rat

The metabolism of [3H]noradrenaline by uterine horns from estrous, ovariectomized, and ovariectomized and estrogen-treated rats was explored. Uterine strips are able to take up [3H]noradrenaline and store it for up to 85 min after the isotope incubation. The major compounds retained in the tissue from rats either ovariectomized or during estrus were [3H]noradrenaline and 3H-labelled O-methylated deaminate metabolites. The [3H]3,4-dihydroxyphenylglycol ([3H]DOPEG) and 3,4-dihydroxymandelic acid ([3H]DOMA) fractions were higher in tissues from ovariectomized rats than during estrus. Ovariectomy also increased significantly the spontaneous efflux of [3H]DOPEG and [3H]DOMA. The metabolic pattern of [3H]noradrenaline both in the tissue and in the efflux from ovariectomized rats was changed by estrogen treatment and became similar to those obtained during estrus. These data suggest a possible modulation of the noradrenaline metabolism by estrogens on the isolated rat uterus.

IONIC STUDIES OF DECIDUAL TISSUE GROWTH IN THE RAT UTERUS

1962 ◽  
Vol 40 (3) ◽  
pp. 472-480 ◽  
Author(s):  
T. Randall Wrenn ◽  
Helene C. Cecil ◽  
Mary R. Connolly ◽  
Joel Bitman
Keyword(s):  
Ionic Composition ◽  
Tissue Growth ◽  
Ovariectomized Rats ◽  
Rat Uterus ◽  
Water Imbibition ◽  
Decidual Tissue ◽  
Inflammatory State ◽  
Composition Characteristic ◽  
Intact Rats

ABSTRACT The sequence of water and electrolyte events was followed during development of deciduoma in rats. Minutes after scratching the endometrium, Na and Cl increased and K decreased, probably due to the influx of blood. These changes were followed by a water imbibition at 24 and 48 hours and an increase in K. Damaged horns increased in weight and width during this period. Water and electrolyte alterations were similar in ovariectomized rats. ovariectomized rats given progesterone, and intact rats during the first 48 hours, suggesting that these initial effects were due to the inflammation resulting from the injury inflicted. During the next 48 hours injured uterine horns of ovariectomized rats regressed and composition approached that of uninjured tissue. In contrast, composition of injured horns of progesterone rats or rats with intact ovaries was markedly different from uninjured tissue. Decidual tissue H2O, K, Na and Cl were significantly higher than in undeveloped uterine horns. Ionic composition characteristic of decidual growth was remarkably similar to the ionic pattern exhibited during the inflammatory state.

Effect of ovarian steroid hormones and the presence of the fetus on oxytocin gene expression in the uterus

1995 ◽  
Vol 146 (1) ◽  
pp. 81-85 ◽  
Author(s):  
T Higuchi ◽  
C-X Liu ◽  
H Saito ◽  
H Negoro ◽  
S Matsukawa
Keyword(s):  
Steroid Hormones ◽  
Blot Hybridization ◽  
Physiological Role ◽  
Ovariectomized Rats ◽  
Dot Blot ◽  
Ovarian Steroid ◽  
Rat Uterus ◽  
Pregnant Uterus ◽  
Posterior Pituitary Gland ◽  
Very High

Abstract Oxytocin (OT) is a neurohypophysial hormone with potent stimulating activity of the pregnant uterus, but its physiological role in parturition is still unclear. Recently, OT was found to be synthesized in the pregnant uterus, indicating that OT originating from the uterus, not from the posterior pituitary gland, may trigger the onset of labour. In order to define the factors responsible for the induction of uterine OT, the effect of ovarian steroid hormones and conceptus on the induction of OT mRNA in the rat uterus was examined by Northern and dot blot hybridization analysis. OT mRNA in the uterus started to increase on day 14 of pregnancy and showed very high levels at the time of parturition. Uterine OT mRNA was not altered by any steroid treatment, oestradiol-17β (0·2 μg), progesterone (4 mg) or both in combination, for 6 days. The gravid horn of the uterus had 3·6-fold as much OT mRNA as the non-gravid horn on day 21 of pregnancy in hemipregnant rats with one ligated oviduct. The ovarian steroid hormones could not induce accumulation of OT mRNA in the uterus of ovariectomized rats, at least under the conditions used, but the presence of a conceptus may be critical for the very high levels of OT mRNA. Journal of Endocrinology (1995) 146, 81–85

LIBERATION OF 5-HYDROXYTRYPTAMINE AND HISTAMINE IN THE ANAPHYLACTIC REACTION OF THE RAT

1961 ◽  
Vol 39 (2) ◽  
pp. 403-416 ◽  
Author(s):  
H. Garcia-Arocha
Keyword(s):  
Small Intestine ◽  
Anaphylactic Reaction ◽  
Cell Suspensions ◽  
Peritoneal Cell ◽  
Rat Tissues ◽  
Uterine Tissue ◽  
Rat Uterus ◽  
Anaphylactic Response

5-Hydroxytryptamine (5-HT) and histamine were released when antigen was added to peritoneal cell suspensions or to the fluid perfusing the small intestine of sensitized rats. In parallel experiments with minced uterine tissue the antigen failed to release significant amounts of histamine into the suspending medium but did release 5-HT. These results, taken in conjunction with the effects of the two amines and of their specific antagonists, suggest that the anaphylactic response of the rat uterus depends on the release of 5-HT, whereas both histamine and 5-HT participate in the anaphylactic response of other rat tissues.

scholarly journals Interaction of nistranol with estradiol and progesterone receptors in the rat uterus cytosol in vitro and in vivo

1996 ◽  
Vol 42 (4) ◽  
pp. 33-34
Author(s):  
Ye. N. Kareva ◽  
V. P. Fedotov ◽  
V. M. Rzheznikov ◽  
Ye. V. Solovyova ◽  
Ye. V. Pokrovskaya
Keyword(s):  
Single Injection ◽  
Progesterone Receptors ◽  
Uterine Tissue ◽  
Rat Uterus ◽  
Cytosol Fraction ◽  
Receptor Complexes ◽  
Target Organs ◽  
Uterine Growth

Interactions between nistranol and estradiol and progesterone receptors in the cytosol fraction of the uterine tissue of oophorectomized rats and the relative competitive capacity of nistranol have been studied 24 h after a single injection of the drug. The results demonstrate the effects of nistranol on estradiol and progesterone binding. Nistranol boosting of uterine growth in rats is explained by its capacity to accelerate the translocation of hor- mone-receptor complexes into the nucleus. Investigations of the capacity of new estrogens to compete with estradiol for binding in the tissues of target organs in vitro and affect estradiol and progesterone binding in vivo permit a more effective screening of estrogens than use of only the classical in vitro method.

MODULATION OF CYCLIC AMP IN ISOLATED RAT UTERINE TISSUE SLICES BY PORCINE RELAXIN

1980 ◽  
Vol 87 (1) ◽  
pp. 153-159 ◽  
Author(s):  
D. G. JUDSON ◽  
SARAH PAY ◽  
K. D. BHOOLA
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Time Course ◽  
Tissue Concentration ◽  
Uterine Tissue ◽  
Tissue Slices ◽  
Rat Uterus ◽  
Hormonal Action ◽  
Isolated Rat

Porcine relaxin produced a rapid, dose-related rise of cyclic AMP values in rat uterine tissue incubated in vitro. In time-course experiments, peak cyclic AMP concentrations were observed in the uterine slices at 5 min; subsequently the values fell, at first rapidly and then more slowly with the tissue concentration remaining significantly raised at 15 min. Levels of cyclic GMP in the same tissue slices were not significantly altered by relaxin. Furthermore, no increase in basal cyclic AMP values was measured in control slices prepared from the rat heart or jejunum. An increase in cyclic AMP concentration comparable to that found in the rat uterus was observed in slices of porcine uterus and cervix but not of vagina when they were stimulated with porcine relaxin. Our results suggest that the hormonal action of relaxin on the uterus and cervix is mediated through receptors linked to the enzyme, adenylate cyclase.

scholarly journals PEROXIDASE-ASSOCIATED BINDING OF ESTRADIOL BY THE RAT UTERUS

1969 ◽  
Vol 17 (6) ◽  
pp. 394-407 ◽  
Author(s):  
JOST BRÖKELMANN
Keyword(s):  
Hydrogen Peroxide ◽  
Incubation Medium ◽  
Degradation Products ◽  
Uterine Tissue ◽  
Electron Microscopic ◽  
Rat Uterus ◽  
In Vitro Binding ◽  
Vitro Binding ◽  
Specific Granules

It has been shown that the addition of hydrogen peroxide to the incubation medium greatly increases the in vitro binding of tritiated estradiol or its degradation products to uterine tissue. The binding probably is covalent. It involves a hemoprotein which can be demonstrated histochemically by a peroxidase reaction. In light and electron microscopic radioautographs, the sites of radioactivity in uterine tissues after incubation with tritiated estradiol are mainly the specific granules of eosinophils and the cytoplasm of epithelial cells. The possible significance of an interaction between estradiol and a peroxidative hemoprotein is discussed.

scholarly journals Expression of the oxytocin gene, but not the vasopressin gene, in the rat uterus during pregnancy: influence of oestradiol and progesterone

2007 ◽  
Vol 193 (1) ◽  
pp. 121-126 ◽  
Author(s):  
T Bossmar ◽  
N Osman ◽  
E Zilahi ◽  
M A El Haj ◽  
N Nowotny ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ovariectomized Rats ◽  
Rat Uterus ◽  
Mrna Accumulation ◽  
Stimulatory Action ◽  
Pregnant Rats ◽  
Reverse Transcription Pcr ◽  
Steroid Dependent ◽  
Endocrine Mechanism ◽  
Vasopressin Gene

Oxytocin (OT) and vasopressin (VP) are neurohypophyseal hormones with potent stimulatory actions on the uterus. In order to determine whether these hormones may have a paracrine action on the uterus, OT and VP gene expression was studied in myometrium from pregnant rats at gestational ages of 14 and 20 days, and from ovariectomized animals treated with oestradiol and progesterone. OTand VP mRNA concentrations were measured using real-time quantitative reverse transcription-PCR, and OT- and VP-like immunoreactivities were determined using RIA. OT mRNA was detected in the uterus from pregnant rats, but did not differ between the groups of different gestational ages. Oestradiol significantly (P<0.05) stimulated OT gene expression in ovariectomized rats. Progesterone alone was without effect on OT mRNA concentrations, but significantly (P<0.05) reduced the oestradiol-induced OT mRNA accumulation. The OT-like immunoreactivity in an extract of myometrium from pregnant rats was eluted from a reverse-phase HPLC column with a retention time identical to that of synthetic OT. Neither VP mRNA nor VP-like immunoreactivity was detected in the myometrium from pregnant or ovariectomized rats. The study demonstrates steroid-dependent expression of the OT gene in the rat uterus and processing of uterine preprooxytocin to the mature nonapeptide. The data support the theory that this peptide may act in a paracrine pathway. No evidence was found for the presence of VP in the uterus so that, if the hormone is involved in a stimulatory action on this tissue, it probably acts via an endocrine mechanism.

Sign in / Sign up

Export Citation Format

Share Document