THE SITES OF IODINE ACCUMULATION AND THE IODOAMINO ACIDS IN THE THYROID AND PLASMA OF PLAICE (PLEURONECTES PLATESSA L.)

1969 ◽  
Vol 43 (4) ◽  
pp. 643-NP ◽  
Author(s):  
R. H. OSBORN ◽  
T. H. SIMPSON
Keyword(s):  
Thin Layer ◽  
Blood Plasma ◽  
Thin Layer Chromatography ◽  
Thyroid Tissue ◽  
Whole Body ◽  
Thyroid Activity ◽  
Body Scanning ◽  
Pleuronectes Platessa ◽  
Layer Chromatography

SUMMARY Whole body scanning and radioautography of plaice injected with 125I failed to show extra-pharyngeal sites of thyroid activity. The constituent iodoamino acids of thyroid tissue and of blood plasma have been identified by thin-layer chromatography of the parent compounds and of their derivatives, the preparation of which is described. Thyroid tissue has been found to contain 3,5,3′-tri-iodothyronine (T3), thyroxine (T4), 3-iodotyrosine (MIT) and 3,5-di-iodotyrosine (DIT). T3 and T4 were the only iodoamino acids detected in plasma.

Porphyrins in Erythrocytes and Blood Plasma: Preliminary Studies with Qualitative Thin-Layer Chromatography

1977 ◽  
Vol 52 (5) ◽  
pp. 463-467 ◽  
Author(s):  
T. K. With
Keyword(s):  
Thin Layer ◽  
Blood Plasma ◽  
Thin Layer Chromatography ◽  
Chromatographic Method ◽  
Erythropoietic Protoporphyria ◽  
Layer Chromatography ◽  
In Blood Plasma

1. A simple chromatographic method for the study of porphyrins in erythrocytes and blood plasma, requiring little technician time, is described. The findings in adults and neonates are presented as well as those from severely ill patients. 2. The regular occurrence of uro-, copro- and proto-porphyrin in erythrocytes is demonstrable as well as sporadic occurrence of tri-, penta-, hexa- and hepta-carboxylic porphyrin. The method gives, however, irregular values for protoporphyrin, which seems to occur in a more labile form in some subjects than in others; this is especially the case in erythropoietic protoporphyria. 3. In blood plasma, uroporphyrin occurs regularly, and coproporphyrin less regularly. Protoporphyrin and penta- to hepta-carboxylic porphyrin are found only occasionally.

Effects of Schistosoma mansoni infection on lutein and β-carotene concentrations in Biomphalaria glabrata snails as determined by quantitative high performance reversed phase thin-layer chromatography

2013 ◽  
Vol 58 (3) ◽  
Author(s):  
Nicole Dieterich ◽  
Bernard Fried ◽  
Joseph Sherma
Keyword(s):  
Thin Layer ◽  
Schistosoma Mansoni ◽  
Thin Layer Chromatography ◽  
Post Infection ◽  
High Performance ◽  
Biomphalaria Glabrata ◽  
Reversed Phase ◽  
Whole Body ◽  
Layer Chromatography ◽  
Β Carotene

AbstractHigh performance thin-layer chromatography was used to determine the concentration of β-carotene and lutein in the whole body and digestive gland-gonad complex (DGG) of uninfected Biomphalaria glabrata snails and those infected with Schistosoma mansoni for 6 and 8 weeks. Pigments were extracted from the snails using acetone and separated on EMD Millipore reversed phase C-18 plates with concentration zone using petroleum ether-acetonitrile-methanol (1:1:2) mobile phase. After development, two yellow pigment zones, lutein and β-carotene, were identified with respective R f values of 0.55 and 0.13 and then quantified by densitometry. Statistical analysis of the weight percentages of each pigment showed a significant decrease (P < 0.05) in the concentration of β-carotene in the DGGs of infected B. glabrata at 6 and 8 weeks post-infection compared to the uninfected snails. No significant differences were seen in the concentrations of β-carotene in the whole body of the uninfected versus infected snail samples. Changes in the lutein concentration of the infected DGG and whole snail bodies were insignificant compared to the uninfected controls. In conclusion, larval S. mansoni infection caused a significant decrease in the β-carotene concentration of the DGG at 6 and 8 weeks post infection.

Thin-Layer Chromatography

1965 ◽  
Author(s):  
H. R. Bolliger ◽  
M. Brenner ◽  
H. Gänshirt ◽  
Helmut K. Mangold ◽  
H. Seiler ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

THE LESS-POLAR METABOLITES PRODUCED BY INCUBATION OF TESTOSTERONE-4-14C WITH RAT AND BOVINE BRAIN

1969 ◽  
Vol 61 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Leon J. Sholiton ◽  
Emile E. Werk
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Paper Chromatography ◽  
Specific Activity ◽  
Bovine Brain ◽  
Polar Fraction ◽  
End Products ◽  
Polar Metabolites ◽  
Layer Chromatography ◽  
Constant Specific Activity

ABSTRACT Rat and bovine brain have been incubated with testosterone-4-14C under standard conditions. With use of paper chromatography, the extracted metabolites were noted to fall into less-polar, iso-polar, and more polar fractions. The components of the less-polar fraction were separated by acetylation and thin-layer chromatography and the major end-products identified by recrystallization to constant specific activity or constant 3H/14C ratios. Androst-4-enedione and 5α-dihydrotestosterone were formed consistently under the conditions utilized. Trace amounts of other less-polar metabolites were noted occasionally.

Sign in / Sign up

Export Citation Format

Share Document