URINARY EXCRETION OF LUTEINIZING HORMONE IN BOYS AND ADULT MEN

1970 ◽  
Vol 46 (2) ◽  
pp. 229-236 ◽  
Author(s):  
N. SCIARRA ◽  
U. LEONE
Keyword(s):  
Luteinizing Hormone ◽  
Urinary Excretion ◽  
Haemagglutination Inhibition ◽  
Adult Child ◽  
Mean Value ◽  
Sharp Rise ◽  
Adult Men ◽  
Immunological Method ◽  
The Mean

SUMMARY The daily urinary excretion of luteinizing hormone (LH) was determined in 15 boys, aged 5–11 yr., and in 15 adult men, aged 18–65 yr., by an immunological method using the haemagglutination inhibition system. The hormone was detected in every subject investigated. The mean value for urinary LH excretion in boys was equivalent to 3·4 i.u./24 hr. (range 1·3–6·5) and was 29·3 i.u./24 hr. in adults (range 15·4–44·6). The mean adult: child ratio was 8·6. There was a significant increase in LH output with age in both the boys and the men; the rate of this increase was the same in both groups. However, there was a sharp rise in hormone output at about the onset of puberty.

ENZYMATISCH-FLUOROMETRISCHE BESTIMMUNG DER CORTISOLBINDUNGSKAPAZITÄT DES PLASMA

1967 ◽  
Vol 56 (1) ◽  
pp. 99-106 ◽  
Author(s):  
K. Leybold ◽  
J. Rieper ◽  
L. Weissbecker
Keyword(s):  
Binding Capacity ◽  
Liver Microsomes ◽  
Mean Value ◽  
Female Rats ◽  
Plasma Samples ◽  
Simple Method ◽  
Adult Men ◽  
The Mean ◽  
Precision And Accuracy

ABSTRACT A simple method for the determination of cortisol-binding capacity is described. For saturation of the cortisol-binding proteins, plasma samples are incubated with an excess of cortisol. In the next step NADPH and liver microsomes of female rats are added. The microsomal Δ4-3-ketosteroid hydrogenase only reduces non protein-bound cortisol to tetrahydrocortisol-5α. Then the steroids are extracted by dichloromethane, and after some purification steps analyzed by fluorometry. Tetrahydrocortisol gives practically no fluorescence. The cortisol determined by this method corresponds to protein-bound cortisol and indicates the extent of cortisolbinding capacity. Precision and accuracy of the method were found to be good. The values of cortisol-binding capacity obtained by our method are compared with the results of other authors. The mean value of adult men was 25.5 ± 3.4 μg/100 ml, that of pregnant women, mens IX-X, 42.3 ± 4.2 μg/100 ml.

EFFECTS OF CONTINUOUS DAILY ADMINISTRATION OF 0.5 mg OF CHLORMADINONE ACETATE ON THE PLASMA LEVELS OF PROGESTERONE AND ON THE URINARY EXCRETION OF LUTEINIZING HORMONE AND TOTAL OESTROGENS

1970 ◽  
Vol 63 (4) ◽  
pp. 705-716 ◽  
Author(s):  
U. Larsson-Cohn ◽  
E. D. B. Johansson ◽  
L. Wide ◽  
C. Gemzell
Keyword(s):  
Luteinizing Hormone ◽  
Plasma Level ◽  
Urinary Excretion ◽  
Corpus Luteum ◽  
Plasma Levels ◽  
Chlormadinone Acetate ◽  
The Mean ◽  
Low Levels ◽  
The Difference ◽  
All Treatment

ABSTRACT Daily determinations of the plasma level of progesterone and the urinary excretion of luteinizing hormone (LH) and total oestrogens were performed in 6 subjects during one control cycle, immediately followed by three cycles of daily treatment with 0.5 mg of chlormadinone acetate continuously. The control cycles were ovulatory according to the parameters investigated. Two of the women showed a normal LH excretion pattern in all treatment cycles. The four other subjects also had periodical variations in the LH excretion but no distinct midcycle peaks occurred. The mean oestrogen excretion was increased in all three treatment cycles but the difference was satistically significant only in the last two cycles. Compared with the treatment cycles, the sum of progesterone values was significantly decreased in the first two cycles. Chlormadinone acetate in this dose had no thermogenic effect. Three of the subjects showed bleeding irregularities which had no clear connection with the hormone variations measured in the study. It is suggested that the low levels of progesterone might be due to a defective corpus luteum function.

AN IMMUNOLOGICAL PREGNANCY TEST

1960 ◽  
Vol XXXV (II) ◽  
pp. 261-267 ◽  
Author(s):  
Leif Wide ◽  
Carl A. Gemzell
Keyword(s):  
Urinary Excretion ◽  
Early Pregnancy ◽  
Human Urine ◽  
Haemagglutination Inhibition ◽  
Chorionic Gonadotrophin ◽  
Menstrual Period ◽  
Pregnancy Test ◽  
Immunological Method ◽  
Inhibition Reaction

ABSTRACT An immunological method for the assay of chorionic gonadotrophin (HCG) in human urine has been described in detail. The method is useful as a simple and rapid pregnancy test and can be applied for quantitative determinations of HCG in urine. Morning urine from 306 women were examined; 212 were found to be pregnant by the haemagglutination inhibition reaction and the pregnancy was confirmed by ordinary pregnancy tests; 94 women were not pregnant and the urine of these women gave in no case a haemagglutination inhibition reaction. Quantitative determinations of HCG were performed in the urine of 103 women in early pregnancy. The urinary excretion of HCG increased following the missed menstrual period and reached in the 8th week of pregnancy a level of about 160.000 IU of HCG per liter of urine.

LUTEINIZING HORMONE (LH) AND PROGESTERONE IN PLASMA AND LH AND OESTROGENS IN URINE DURING 42 NORMAL MENSTRUAL CYCLES

1971 ◽  
Vol 68 (3) ◽  
pp. 502-512 ◽  
Author(s):  
Elof D. B. Johansson ◽  
Leif Wide ◽  
Carl Gemzell
Keyword(s):  
Luteinizing Hormone ◽  
Urinary Excretion ◽  
Plasma Levels ◽  
Luteal Phase ◽  
Maximum Level ◽  
Plasma Progesterone ◽  
Menstrual Cycles ◽  
Normal Menstrual Cycle ◽  
The Mean ◽  
Second Peak

ABSTRACT The plasma levels of luteinizing hormone (LH) and progesterone and the urinary excretion of LH and oestrogens were measured during the normal menstrual cycle of 22 young and healthy women. A total of 42 cycles were investigated. The urinary excretion of total oestrogens increased during several days before the rise of LH in the urine. The day of maximum excretion of LH and total oestrogens coincided during the midcycle period. The mid-cyclic rise in LH was found to occur on the same day in the plasma and urine and the days of maximum values coincided in 11 out of 16 cycles. In 5 cycles the maximum level was reached one day later in the urine. The plasma levels of progesterone started to increase during the LH and oestrogen peaks. The days for maximum levels of progesterone coincided with the second peak of urinary oestrogens. The levels of progesterone in the plasma reached values above 10 ng per ml in all normal cycles. The plasma levels of progesterone were below 1 ng per ml plasma when menstrual bleeding started. The length of the luteal phase was 14.4 ± 1.1 (s) days. The sum of daily urinary excretion of total oestrogens and the sum of the daily plasma progesterone levels varied within 15 per cent of the mean in five out of six women studied during more than two cycles. The variation in values for the sums of daily oestrogen excretion and plasma progesterone levels was considerably larger between menstrual cycles of different women than between menstrual cycles of the same woman.

IMMUNOLOGICAL DETERMINATION OF PITUITARY LUTEINIZING HORMONE IN THE URINE OF FERTILE AND POST-MENOPAUSAL WOMEN AND ADULT MEN

1962 ◽  
Vol 39 (4) ◽  
pp. 539-546 ◽  
Author(s):  
Leif Wide ◽  
Carl Gemzell
Keyword(s):  
Luteinizing Hormone ◽  
Haemagglutination Inhibition ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Adult Men ◽  
Menopausal Women ◽  
Post Menopausal ◽  
Inhibition Reaction

ABSTRACT An immunological method to assay human pituitary luteinizing hormone (HPLH) in urine is described. It is based on the fact that HPLH crossreacts with human chorionic gonadotrophin (HCG) in an haemagglutination inhibition reaction between HCG-coated blood cells and rabbit HCG-antisera. During the menstrual cycle the excretion of HPLH reached a peak of 200–400 U per liter at the time of ovulation. In the urine of post-menopausal women the concentration of HPLH was between 100 and 400 U per liter. In the urine of adult men the concentration of HPLH was between 50 and 160 U per liter.

THE EXCRETION OF HUMAN CHORIONIC GONADOTROPHIN IN NORMAL PREGNANCY. AN IMMUNOLOGICAL INVESTIGATION

1964 ◽  
Vol 30 (1) ◽  
pp. 79-86 ◽  
Author(s):  
AILEEN F. CONNON
Keyword(s):  
Haemagglutination Inhibition ◽  
Normal Pregnancy ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Confidence Limits ◽  
Mean Values ◽  
Immunological Method ◽  
Immunological Investigation ◽  
The Mean ◽  
Immunological Assays

SUMMARY The haemagglutination-inhibition test was investigated as an immunological method for the assay of human chorionic gonadotrophin (HCG). The preparation of a potent antiserum to HCG is described. The results of the assay of HCG during normal pregnancy in ten patients are compared with the results of immunological assays from two Swedish laboratories. The mean values and the 95 % confidence limits for the excretion of HCG from the 10th week of normal pregnancy are calculated.

Effects of acute stimulation with luteinizing hormone-releasing hormone (LRH) on biologically active and immunoreactive serum luteinizing hormone (LH) in pubertal boys

1984 ◽  
Vol 107 (3) ◽  
pp. 289-294 ◽  
Author(s):  
Vanna Montanini ◽  
Marco Francesco Celani ◽  
Gian Franco Baraghini ◽  
Cesare Carani ◽  
Paolo Marrama
Keyword(s):  
Luteinizing Hormone ◽  
Serum Levels ◽  
Biologically Active ◽  
Acute Administration ◽  
Adult Men ◽  
Serum Luteinizing Hormone ◽  
Significant Difference ◽  
The Mean ◽  
Pubertal Boys

Abstract. The responses of biologically active LH (BIO-LH) and immunoreactive LH (RIA-LH) to acute stimulation with LRH (0.1 mg iv) were studied in 8 pubertal boys (9–15 years, 2nd–4th Tanner's stage), and in 10 healthy adult men (20–46 years). Serum levels of BIO-LH were assessed by an in vitro bioassay method based upon testosterone production by mechanically dispersed mouse Leydig cell preparations. In pubertal boys the mean BIO-LH/RIA-LH (B/I) ratio of basally secreted LH was significantly lower than in adult men (1.2 ± 0.2 (sem) and 2.2 ± 0.2 respectively, P < 0.01). After acute administration of LRH the mean B/I ratio of circulating LH showed a significant increase from the basal value in pubertal boys (2.6 ± 0.2, P < 0.01 vs basal values), whereas no significant difference in LH B/I ratios were demonstrated throughout the study period in adult men (2.1 ± 0.1, P = NS vs basal values). In agreement with this finding, the mean relative maximum response for BIO-LH (BIO-LH Δ%) was higher in pubertal boys than in adult men (1702.7 ± 500.3 and 499.6 ± 65.4% respectively, P < 0.05), whereas the mean RIA-LH Δ% was similar in both groups (609.1 ± 85.1 and 534.1 ± 75.5% respectively, P = NS). No significant differences were shown in the BIO-LH Δ area between pubertal boys (4.9 ± 0.9 area units × 103) and adult men (6.7 ± 1.2 area units × 103, P = NS), whereas the mean RIA-LH Δ area was significantly lower in the former group (1.9 ± 0.4 area units × 103 vs 3.2 ± 0.5 area units × 103, P < 0.05). Our study emphasizes that the pubertal pituitary possesses a greater responsiveness for BIO-LH than the adult pituitary, and that in pubertal boys acute stimulation with LRH evokes the release of a more bioactive form of LH.

scholarly journals Fluorometric Assay of Human Serum Carnosinase Activity in Normal Children, Adults and Patients with Myopathy

1984 ◽  
Vol 21 (6) ◽  
pp. 510-514 ◽  
Author(s):  
K Bando ◽  
T Shimotsuji ◽  
H Toyoshima ◽  
C Hayashi ◽  
K Miyai
Keyword(s):  
Muscular Dystrophy ◽  
Human Serum ◽  
Accurate Method ◽  
Mean Value ◽  
Adult Women ◽  
Normal Children ◽  
Adult Men ◽  
The Mean ◽  
Low Activity ◽  
Normal Adults

A sensitive, accurate method has been established for the assay of serum carnosinase by measuring the fluorescence emitted from the L-histidine liberated on treatment with o-phthaldialdehyde. Using this method the serum values for normal adults, infants and children were measured. The mean value was very low in infants of less than 1 year old but increased with age, being almost the same in children aged 6 years or more as in adults. In adult men, the mean activity was 1·85 μmol/mL/h and in adult women it was 2·07 μmol/mL/h. Low activity was observed in patients with muscular dystrophy.

THE URINARY EXCRETION OF GONADOTROPHINS BY SHEEP: THE EFFECT OF SOME NUTRITIONAL FACTORS ON THE EXCRETION OF GONADOTROPHINS BY CASTRATED MALE SHEEP

1964 ◽  
Vol 29 (3) ◽  
pp. 229-234 ◽  
Author(s):  
R. B. SYMINGTON
Keyword(s):  
Luteinizing Hormone ◽  
Urinary Excretion ◽  
Urine Output ◽  
Follicle Stimulating Hormone ◽  
Temporal Fluctuation ◽  
Nutritional Factors ◽  
Daily Output ◽  
Merino Sheep ◽  
The Mean ◽  
Male Sheep

SUMMARY Male castrated Merino sheep were fed indoors on a dry ration or grazed on a good quality pasture. Despite the much greater urine volumes excreted by the grazing animals, the mean daily rates of excretion of total gonadotrophic activity and follicle-stimulating hormone (FSH) were very similar in the two groups. Corresponding levels of excretion of luteinizing hormone (LH) were very variable, owing apparently to deficiencies in the assay technique. When sheep were fed indoors on freshly cut grass, the urine output was almost three-quarters of that of grazing animals but the rate of excretion of total gonadotrophic activity and of FSH was considerably greater than that of grazing animals. The daily output of urine by sheep fed indoors on a dry ration was adjusted to equal that of grazing sheep. Although the amounts of total gonadotrophic activity were smaller than those in the previous experiments, the amounts of FSH excreted were the highest recorded in the entire investigation. The possibility of a temporal fluctuation in the excretion of gonadotrophins is discussed.

OBSERVATIONS ON A HAEMAGGLUTINATION—INHIBITION TECHNIQUE FOR ESTIMATING HUMAN GROWTH HORMONE

1963 ◽  
Vol 26 (2) ◽  
pp. 219-231 ◽  
Author(s):  
P. J. O'CONNOR ◽  
L. G. SKINNER
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Haemagglutination Inhibition ◽  
Human Growth ◽  
Mean Value ◽  
Normal Adult ◽  
Adult Human ◽  
Human Sera ◽  
The Mean ◽  
Inhibition Technique

SUMMARY The haemagglutination—inhibition technique has been examined as a method of estimating human growth hormone (HGH) and the need for rigid standardization of the procedures involved is stressed. Examination of antisera to a Raben type preparation by immunodiffusion and haemagglutination—inhibition procedures showed the presence of antibodies to albumin and γ-globulin as well as to HGH. The presence of these contaminating antibodies did not appear to interfere with the endpoints obtained in the haemagglutination—inhibition reactions. Within its limitations the technique has been found suitable for the assay of solutions of purified HGH. The mean level of HGH in six normal adult human sera was estimated as 261 ± 23·6 μg./l. (± s.e.) which is similar to the values obtained by other workers, but the validity of this mean value is questioned.

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