Interaction between prolactin and zinc in the human prostate gland

1984 ◽  
Vol 102 (1) ◽  
pp. 73-76 ◽  
Author(s):  
A. Leake ◽  
G. D. Chisholm ◽  
F. K. Habib
Keyword(s):  
Specific Binding ◽  
Prostate Gland ◽  
Benign Prostatic Hypertrophy ◽  
Peptide Hormone ◽  
Prostatic Hypertrophy ◽  
Human Prostate ◽  
Binding Properties ◽  
Competitive Mechanism ◽  
Human Prolactin

ABSTRACT The interaction between prolactin and zinc was examined in vitro in the human prostate gland. The results indicated that prolactin did not modulate the acute uptake of zinc into benign prostatic hypertrophy tissue whereas zinc, in contrast, increased the uptake of prolactin into the prostate gland. Our study further showed that the augmented uptake of prolactin by zinc was partly due to an increase in the non-specific binding properties of the peptide hormone. We were also able to demonstrate that the specific binding of 125I-labelled human prolactin to the receptor was reduced in the presence of zinc by a competitive mechanism. J. Endocr. (1984) 102, 73–76

SECRETION OF DIHYDROTESTOSTERONE BY HUMAN PROSTATE IN BENIGN PROSTATIC HYPERTROPHY

1974 ◽  
Vol 77 (2) ◽  
pp. 401-407 ◽  
Author(s):  
J. A. Mahoudeau ◽  
A. Delassalle ◽  
H. Bricaire
Keyword(s):  
Plasma Levels ◽  
Benign Prostatic Hypertrophy ◽  
Prostatic Hypertrophy ◽  
Human Prostate ◽  
Control Subjects ◽  
Peripheral Plasma ◽  
Significant Difference ◽  
Before And After ◽  
The Mean ◽  
And Control

ABSTRACT Plasma levels of testosterone (T) and 5α-dihydrotestosterone (DHT) were determined by radioimmunoassay in 29 patients with benign prostatic hypertrophy (BPH) and in 56 control men of various ages. No significant difference was found in T, DHT nor DHT/T ratio between BPH and control subjects of similar age. Plasma DHT was higher in the prostatic than in the peripheral veins in 8/9 patients with BPH during laparotomy, indicating a prostatic secretion of DHT. No difference in the mean T nor the mean DHT was found in peripheral plasma before and after adenomectomy.

IN-VITRO STUDIES ON TESTOSTERONE AND 5α-DIHYDROTESTOSTERONE BINDING IN BENIGN PROSTATIC HYPERTROPHY (BPH)

1973 ◽  
Vol 71 (4_Suppl) ◽  
pp. S69
Author(s):  
P. Steins ◽  
H. J. Hollmann ◽  
H. Schmidt ◽  
K. D. Voigt
Keyword(s):  
Benign Prostatic Hypertrophy ◽  
Prostatic Hypertrophy ◽  
In Vitro Studies

IN VITRO STUDIES OF TESTOSTERONE AND 5α-DIHYDROTESTOSTERONE BINDING IN BENIGN PROSTATIC HYPERTROPHY

1974 ◽  
Vol 75 (4) ◽  
pp. 773-784 ◽  
Author(s):  
P. Steins ◽  
M. Krieg ◽  
H. J. Hollmann ◽  
K. D. Voigt
Keyword(s):  
High Speed ◽  
Biological Fluids ◽  
Benign Prostatic Hypertrophy ◽  
Prostatic Hypertrophy ◽  
Sex Hormone Binding Globulin ◽  
List Type ◽  
Plasma Samples ◽  
Experimental Conditions ◽  
Displacement Experiments

ABSTRACT The cytosol fractions of prostatic adenoma and of the rectus abdominis muscle, together with the respective plasma samples were investigated in 13 patients in order to clarify whether or not there is an androgen receptor in human benign prostatic hypertrophy. The following methods were used: High speed ultracentrifugation in a sucrose gradient and agar gel electrophoresis at low temperature, of the 100 000 g supernatants after in vitro incubation with 3H-testosterone, 3H-5α-dihydrotestosterone, and 3H-oestradiol-17β. In parallel experiments the supernatants were heated to 45°C for 1 h before the steroid incubation. Displacement experiments with a 100–500-fold excess of various cold androgens. In the supernatants as well as in the plasma samples the total protein concentration was measured by the biuret reaction. The concentration of albumin and of immunoglobulin G (IgG) in the various biological fluids was determined by quantitative immuno diffusion. After charcoal stripping the binding of testosterone and of 5α-dihydrotestosterone was estimated quantitatively and correlated to the plasma contamination. By the methods used no physico-chemical differences in the androgen binding properties of plasma and of prostate cytosol were observed. However, in any individual cytosol a higher 5α-DHT binding than could be related to the plasma contamination was obtained. The question therefore remains open, as to whether the augmented 5α-dihydrotestosterone binding relates to a cellular increase of sex hormone binding globulin (SHBG) or to androgen receptors which do not show up clearly under the experimental conditions used.

Effects of finasteride on size of the prostate gland and semen quality in dogs with benign prostatic hypertrophy

2001 ◽  
Vol 218 (8) ◽  
pp. 1275-1280 ◽  
Author(s):  
Kaitkanoke Sirinarumitr ◽  
Shirley D. Johnston ◽  
Margaret V. Root Kustritz ◽  
Gary R. Johnston ◽  
Dipak K. Sarkar ◽  
...  
Keyword(s):  
Semen Quality ◽  
Prostate Gland ◽  
Benign Prostatic Hypertrophy ◽  
Prostatic Hypertrophy

Studies on Corticosterone–Receptor Complexes from Mouse Placenta

1974 ◽  
Vol 52 (3) ◽  
pp. 190-195 ◽  
Author(s):  
Ming D. Wong ◽  
A. F. Burton
Keyword(s):  
Binding Sites ◽  
Time Course ◽  
Specific Binding ◽  
Receptor Site ◽  
Binding Properties ◽  
Receptor Complexes ◽  
Specific Binding Sites ◽  
Mouse Placenta ◽  
Vitro Binding

The in vitro binding of radioactive steroids to components of mouse placental nuclei and cytoplasm was investigated using Sephadex or charcoal to remove unbound steroid. Specificity was indicated in competition experiments using excess unlabelled competing steroids. Only the active glucocorticoids formed complexes that could be isolated from the nucleus. The binding properties of the cytoplasmic steroid–receptor complex were studied. From the time course of binding the complex was shown to be more stable at 0° than at 37°, and the distribution of receptors in the cytosol appeared to be homogeneous. The complex was labile to heat and to proteolytic digestion but did not appear to be affected by nucleases or sulfhydryl reagents. Kinetic analysis revealed the presence of high affinity specific binding sites with a dissociation constant of 17.5 nM and a receptor site concentration of 0.26 pmol/mg protein. The corticosterone isolated from nuclear complexes and dexamethasone from cytoplasmic complexes were identified by chromatography and by cocrystallization as the unchanged steroid in each case.

scholarly journals Intraurethral Catheter: Alternative Management for Urinary Retention in Patients With Benign Prostatic Hypertrophy

1995 ◽  
Vol 2 (2) ◽  
pp. 113-117
Author(s):  
Sulabha Punekar ◽  
Prem A. Ramkrishnan ◽  
Anand R. Kelkar ◽  
Jaydeep A. Date ◽  
Vasudeo R. Ridhorkar
Keyword(s):  
Urinary Retention ◽  
Peak Flow ◽  
Prostate Gland ◽  
Benign Prostatic Hypertrophy ◽  
Prostatic Hypertrophy ◽  
Fluoroscopic Control ◽  
Calculus Formation ◽  
Clinically Significant

A polyurethane intraurethral catheter (IUC) was used in 27 patients with benign prostatic hypertrophy who were unfit for surgery, or were awaiting surgery. All of them had previously had a periurethral catheter inserted. The IUC was inserted with a cystoscope under fluoroscopic control. Spontaneous voiding through the IUC resumed in 25 patients (93%) in the immediate postprocedure period. At the end of 6 months follow-up, the peak flow rates and the residual volumes estimated in 22 patients were satisfactory. Immediate complications included incontinence due to distal displacement in 2 patients and hematuria in one patient; long-term complications included mild encrustation of the IUC in 2 patients and calculus formation on the IUC in 1 patient. None of the patients had clinically significant urinary tract infection. The presence of the IUC did not compromise the subsequent transurethral resection of the prostate gland. We recommend the use of an IUC for up to 6 months in patients with urinary retention who are awaiting surgery or are unfit for surgery as an alternative to an indwelling urethral catheter.

scholarly journals Phage Display Selection of an Anti-Idiotype-Antibody with Broad-Specificity to Deoxynivalenol Mycotoxins

Toxins ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 18
Author(s):  
Janne Leivo ◽  
Markus Vehniäinen ◽  
Urpo Lamminmäki
Keyword(s):  
In Vitro Selection ◽  
Specific Binding ◽  
Binding Properties ◽  
Phage Displays ◽  
Synthetic Antibody ◽  
Wide Range ◽  
Antibody Libraries ◽  
Rapid Generation ◽  
Selection Of

The use of synthetic antibody libraries and phage displays provides an efficient and robust method for the generation of antibodies against a wide range of targets with highly specific binding properties. As the in vitro selection conditions can be easily controlled, these methods enable the rapid generation of binders against difficult targets such as toxins and haptens. In this study, we used deoxynivalenol mycotoxin as a target to generate anti-idiotype-antibodies with unique binding properties from synthetic antibody libraries. The binding of the selected anti-idiotype antibodies can be efficiently inhibited with the addition of free isoforms of deoxynivalenol. The antibody was consecutively used to develop deoxynivalenol-specific ELISA and TRF-immunoassays, which can detect deoxynivalenol and two of the most common metabolic isoforms in the range of 78–115 ng/mL.

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