scholarly journals Molecular cloning and differential expression of three GnRH mRNAs in discrete brain areas and lymphocytes in red drum

2006 ◽  
Vol 188 (3) ◽  
pp. 407-416 ◽  
Author(s):  
J Shaik Mohamed ◽  
I A Khan
Keyword(s):  
Mrna Expression ◽  
Sea Bass ◽  
Red Drum ◽  
Sciaenops Ocellatus ◽  
Sea Bream ◽  
Hypothalamic Area ◽  
Cdna Sequences ◽  
Peptide Expression ◽  
Gonadal Recrudescence ◽  
The Brain

Three gonadotropin releasing hormones, seabream GnRH (GnRH-I), chicken GnRH-II (GnRH-II) and salmon GnRH (GnRH-III) cDNAs were isolated from the brain of the red drum, Sciaenops ocellatus. The GnRH cDNA sequences of red drum showed more similarity to those of Atlantic croaker, sea bass and sea bream. The real-time quantitative RT-PCR study revealed expression of GnRH-I and GnRH III mRNAs in the olfactory bulb plus telencephalon (OB+TEL), and preoptic-anterior hypothalamic area (POAH), indicating an overlap of the GnRH-I and GnRH-III systems in these forebrain regions. However, GnRH-II mRNA expression was detected only in the midbrain tegmentum (MT). The GnRH-I mRNA expression in the POAH in fish undergoing gonadal recrudescence was significantly higher than that in gonadally immature individuals, suggesting involvement of the POAH GnRH-I in gonadal maturation. On the other hand, GnRH-III mRNA expression was significantly higher in the OB+TEL region compared with that in the POAH. Moreover, the demonstration of GnRH-I mRNA and peptide expression in red drum lymphocytes indicates that the GnRH-I is synthesized in these cells of the immune system similar to the situation in mammals.

Molecular cloning and regulation of mRNA expression of the thyrotropin β and glycoprotein hormone α subunits in red drum, Sciaenops ocellatus

2010 ◽  
Vol 36 (4) ◽  
pp. 1277-1290 ◽  
Author(s):  
William B. Cohn ◽  
Richard A. Jones ◽  
Roldan A. Valverde ◽  
Kevin A. Leiner ◽  
Duncan S. MacKenzie
Keyword(s):  
Mrna Expression ◽  
Molecular Cloning ◽  
Red Drum ◽  
Sciaenops Ocellatus ◽  
Glycoprotein Hormone ◽  
Α Subunits

Cyclic mRNA expression of thyrotropin subunits and deiodinases in red drum, Sciaenops ocellatus

2013 ◽  
Vol 194 ◽  
pp. 248-256 ◽  
Author(s):  
R.A. Jones ◽  
W.B. Cohn ◽  
T.C. Miller ◽  
J.T. Jaques ◽  
D.S. MacKenzie
Keyword(s):  
Mrna Expression ◽  
Red Drum ◽  
Sciaenops Ocellatus

scholarly journals The GnRH system in the European sea bass (Dicentrarchus labrax)

2002 ◽  
Vol 172 (1) ◽  
pp. 105-116 ◽  
Author(s):  
N Zmora ◽  
D Gonzalez-Martinez ◽  
JA Munoz-Cueto ◽  
T Madigou ◽  
E Mananos-Sanchez ◽  
...  
Keyword(s):  
Striped Bass ◽  
Stop Codon ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Amino Acid Sequences ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
High Homology ◽  
European Sea Bass ◽  
The Brain

The cDNA sequences encoding three GnRH forms, sea bream GnRH (sbGnRH), salmon GnRH (sGnRH) and chicken GnRH II (cGnRH II), were cloned from the brain of European sea bass, Dicentrarchus labrax. Comparison of their deduced amino acid sequences to the same forms in the gilthead sea bream, Sparus aurata, and striped bass, Morone saxatilis, revealed high homology of the prepro-cGnRH II (94% and 98% respectively), and prepro-sGnRH (92% to both species). The sbGnRH exhibited dissimilar identities, with high homology to the striped bass (93%), and lower homology (59%) to the gilthead sea bream. Two transcript types were identified for the GnRH-associated peptide (GAP)-sGnRH as well as for the GAP-cGnRH II, which suggests a possible alternative splicing followed by the addition of an early stop codon. In order to obtain antibodies specific for the three GnRH precursors, recombinant GAP proteins were produced. The differential expression of the three GnRHs previously reported in the brain by means of in situ hybridization, using riboprobes corresponding to the GAP-coding regions, was fully confirmed by immunocytochemistry using antibodies raised against the recombinant GAP proteins, indicating that the transcripts are translated into functional proteins. Moreover, this approach allowed us to follow, for the first time, the specific projections of the different cell groups: sGAP fibers are distributed mainly in the forebrain with few projections reaching the pituitary, sbGAP fibers are mainly present in the preoptic area, mediobasal hypothalamus and predominantly project to the pars distalis of the pituitary, whereas cGnRH II fibers have a widespread distribution primarily in the posterior brain, and do not project to the pituitary. These new tools will be extremely useful to study further the development, regulation and functional significance of three independent GnRH systems in the brain of vertebrate species.

Studies on Prevailing Parasitic Fish Diseases in Pre-mature Cultured Sea bass, Dicentrarchus labrax, Sea bream and Mugil cephalus at Ismailia, Province with Special References to Control

2020 ◽  
Keyword(s):  
Parasitic Infection ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Mugil Cephalus ◽  
Sea Bream ◽  
Fish Diseases ◽  
Total Prevalence ◽  
Dark Colour ◽  
Gill Filaments ◽  
Mature Fish

This study concentrated on the assessment of the prevailing parasitic fish diseases in some marine fishes at Ismailia province and how to control the infestation using microalgae. This study was carried out on 1080 pre-mature fish (360 D. labrax (225±25 g) and 360 S aurata (150±25 g) and 360 M. cephalus (125±25 g) collected from similar ponds of studies to be examined at the end of treatment. In addition to that we followed non-treated fish (1080 premature). The infested fish showed dark colour and respiratory signs. Post mortem lesions were a presence of congestion or paleness and destruction of gill filaments. The total prevalence of infestation was the total prevalence of parasitic infection of non-treated fishes was 45.83 %. The highest percentage was in D. labrax 56.94 % followed by S. aurata 47.22%, the lowest percentage in M. cephalus 33.33. The total prevalence of parasitic infection in premature treated with 2 g algae was 28.79%, followed by 3 g algae was 23.60 %, while the lowest percentage with 5 g algae was 20.37 % respectively. The detected species of parasites were protozoal parasites, Amyloodinium ocellatum and Riboscyphidia in additions of marine monogenea, Lamellodiscus diplodicus isolated from D Labrex, Mugil Cephalus and S aurata. The present study concluded that, the use of microalgae instead of fish meal decreased parasitic infestation in marine fish. The histopathological alteration of natural infested examined fishes was also recorded.

Diagnostic investigation for the detection of mycobacteria in samples of fish feeds and tissue from sea bream and sea bass with severe granulomatous lesions

Aquaculture ◽  
2021 ◽  
pp. 737283
Author(s):  
Antonia Mataragka ◽  
Nikolaos Tzimotoudis ◽  
Markos Kolygas ◽  
Emmanuel Karavanis ◽  
John Ikonomopoulos
Keyword(s):  
Sea Bass ◽  
Sea Bream ◽  
Diagnostic Investigation ◽  
Granulomatous Lesions ◽  
Fish Feeds

First evidence of fish nocardiosis in Mexico caused by Nocardia seriolae in farmed red drum ( Sciaenops ocellatus , Linnaeus)

2021 ◽  
Author(s):  
Rodolfo Enrique Rio‐Rodriguez ◽  
Jose Gustavo Ramirez‐Paredes ◽  
Sonia Araceli Soto‐Rodriguez ◽  
Yechiam Shapira ◽  
Mariana del Jesus Huchin‐Cortes ◽  
...  
Keyword(s):  
Red Drum ◽  
Sciaenops Ocellatus ◽  
Nocardia Seriolae
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