Electrochemical detection of adrenaline and hydrogen peroxide on carbon nanotube electrodes

Adrenaline and hydrogen peroxide have neuromodulatory functions in the brain and peroxide is also formed during reaction of adrenaline. Considerable interest exists in developing electrochemical sensors that can detect their levels in vivo due to their important biochemical roles. Challenges associated with electrochemical detection of hydrogen peroxide and adrenaline are that the oxidation of these molecules usually requires highly oxidising potentials (beyond 1.4 V vs Ag/AgCl) where electrode damage and biofouling are likely and the signals of adrenaline, hydrogen peroxide and adenosine overlap on most electrode materials. To address these issues we fabricated pyrolysed carbon electrodes coated with oxidised carbon nanotubes (CNTs). Using these electrodes for fast-scan cyclic voltammetric (FSCV) measurements showed that the electrode offers reduced overpotentials compared with graphite and improved resistance to biofouling. Adrenaline oxidises on this electrode at 0.75(±0.1) V and reduces back at −0.2(±0.1) V while hydrogen peroxide oxidation is detected at 0.85(±0.1) V on this electrode. The electrodes are highly sensitive with a sensitivity of 16 nA µM−1 for Adrenaline and 11 nA µM−1 for hydrogen peroxide on an 80 µm2 electrode. They are also suitable to distinguish between adrenaline, hydrogen peroxide and adenosine thus these probes can be used for multimodal detection of analytes.

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Electrochemical Detection of Adrenaline and Hydrogen Peroxide on Carbon Nanotube Electrodes

10.1101/2021.08.24.457486 ◽

2021 ◽

Author(s):

Gaurang Khot ◽

Mohsin Kaboli ◽

Tansu Celikel ◽

Neil Shirtcliffe

Keyword(s):

Carbon Nanotubes ◽

Hydrogen Peroxide ◽

Carbon Nanotube ◽

Electrochemical Detection ◽

Electrochemical Sensors ◽

Carbon Electrodes ◽

Cyclic Voltammetric ◽

Highly Sensitive

Adrenaline and hydrogen peroxide have neuromodulatory functions in the brain.Considerable interest exists in developing electrochemical sensors that can detect their levels in vivo due to their important biochemical roles. Challenges associated with electrochemical detection of hydrogen peroxide and adrenaline are that the oxidation of these molecules usually requires highly oxidising potentials (beyond 1.4V vs Ag/AgCl) where electrode damage and biofouling are likely and the signals of adrenaline, hydrogen peroxide and adenosine overlap. To address these issues we fabricated pyrolysed carbon electrodes coated with oxidised carbon nanotubes (CNTs). Using these electrodes for fast-scan cyclic voltammetric (FSCV) measurements showed that the electrode offers reduced overpotentials compared with graphite and improved resistance to biofouling. The Adrenaline peak is reached at 0.75 V and reduced back at -0.2 V while hydrogen peroxide is detected at 0.85V on this electrode. The electrodes are highly sensitive with a sensitivity of16nA microM-1 for Adrenaline and 11nA microM-1 for hydrogen peroxide on an 80 micro m2 electrode. They are also suitable to distinguish between adrenaline, hydrogen peroxide and adenosine thus these probes can be used for multimodal detection of analytes.

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Aptamer-functionalized neural recording electrodes for the direct measurement of cocaine in vivo

Journal of Materials Chemistry B ◽

10.1039/c7tb00095b ◽

2017 ◽

Vol 5 (13) ◽

pp. 2445-2458 ◽

Cited By ~ 19

Author(s):

I. Mitch Taylor ◽

Zhanhong Du ◽

Emma T. Bigelow ◽

James R. Eles ◽

Anthony R. Horner ◽

...

Keyword(s):

Electrochemical Detection ◽

Direct Measurement ◽

Neural Recording ◽

Dna Aptamer ◽

Silicon Based ◽

The Brain ◽

Recording Electrodes

First everin vivosensor for directly measuring cocaine concentration in the brainviaelectrochemical detection at DNA aptamer functionalized single shank, silicon-based neural recording probes.

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Effects of the Coreopsis tinctoria extracts on anti-agingin the aging model mice

Indian Journal of Animal Research ◽

10.18805/ijar.9630 ◽

2016 ◽

Author(s):

Weixin Zhang ◽

Haiyan Hao ◽

Ailong Sha

Keyword(s):

Hydrogen Peroxide ◽

Aging Effects ◽

Once Daily ◽

Aging Model ◽

Coreopsis Tinctoria ◽

Kg Medium ◽

The Brain ◽

Liver Tissues ◽

Brain Tissues

The effects of the Coreopsis tinctoria extracts on anti-aging were observed by investigating the cerebral index and viscera indexes, the contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in the serums, the activities of glutathione peroxidase (GSH-Px) in the brain tissues and the ones of catalase (CAT) and superoxide dismutase (SOD) in the liver tissues of the aging model mice. The aging model mice were injected subcutaneously with D-galactose in vivo and intragastric administrated with the Coreopsis tinctoria extracts at doses of low (0.5g/kg), medium (1g/ kg) and high (2g/ kg) once daily for 6 weeks. The results showed that all the cerebral index, spleen index, thymus index, liver index and kidney index of the three groups dosed of the Coreopsis tinctoria extracts increased, the activities of GSH-Px in the brain tissues and the ones of CAT and SOD in the liver tissues increased to different degree while the contents of H2O2 and MDA in the serums decreased extremely and significantly (P<0.01) compared with the aging model mice. All of these results suggested that the Coreopsis tinctoria extracts might possess anti-aging effects by improving antioxidant capacity of the mice.

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Highly Sensitive Platinum-Black Coated Platinum Electrodes for Electrochemical Detection of Hydrogen Peroxide and Nitrite in Microchannel

Electroanalysis ◽

10.1002/elan.201200456 ◽

2013 ◽

Vol 25 (4) ◽

pp. 895-902 ◽

Cited By ~ 47

Author(s):

Yun Li ◽

Catherine Sella ◽

Fredéric Lemaître ◽

Manon Guille Collignon ◽

Laurent Thouin ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Electrochemical Detection ◽

Platinum Black ◽

Platinum Electrodes ◽

Highly Sensitive

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MnO2 nanorods grown NGNF nanocomposites for the application of highly sensitive and selective electrochemical detection of hydrogen peroxide

Journal of Industrial and Engineering Chemistry ◽

10.1016/j.jiec.2016.09.012 ◽

2017 ◽

Vol 46 ◽

pp. 19-27 ◽

Cited By ~ 23

Author(s):

K. Ramachandran ◽

Awan Zahoor ◽

T. Raj Kumar ◽

Kee Suk Nahm ◽

A. Balasubramani ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Electrochemical Detection ◽

Highly Sensitive

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Latest Trends in Electrochemical Sensors for Neurotransmitters: A Review

Sensors ◽

10.3390/s19092037 ◽

2019 ◽

Vol 19 (9) ◽

pp. 2037 ◽

Cited By ~ 24

Author(s):

Tavakolian-Ardakani ◽

Hosu ◽

Cristea ◽

Mazloum-Ardakani ◽

Marrazza

Keyword(s):

Electrochemical Sensors ◽

Ex Vivo ◽

Analytical Techniques ◽

Substantial Improvement ◽

Robust Detection ◽

Brain Functions ◽

The Brain ◽

Made In

Neurotransmitters are endogenous chemical messengers which play an important role in many of the brain functions, abnormal levels being correlated with physical, psychotic and neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's disease. Therefore, their sensitive and robust detection is of great clinical significance. Electrochemical methods have been intensively used in the last decades for neurotransmitter detection, outclassing more complicated analytical techniques such as conventional spectrophotometry, chromatography, fluorescence, flow injection, and capillary electrophoresis. In this manuscript, the most successful and promising electrochemical enzyme-free and enzymatic sensors for neurotransmitter detection are reviewed. Focusing on the activity of worldwide researchers mainly during the last ten years (2010–2019), without pretending to be exhaustive, we present an overview of the progress made in sensing strategies during this time. Particular emphasis is placed on nanostructured-based sensors, which show a substantial improvement of the analytical performances. This review also examines the progress made in biosensors for neurotransmitter measurements in vitro, in vivo and ex vivo.

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