Determination of the wing morphology differentiation of old and recent honey bee samples from western Turkey using geometric morphometrics

Abstract The effectiveness of two methods of collecting semen from honeybee Apis mellifera drones was compared, and the reasons for problems with ejaculating semen were analysed. Among 275 drones, 100 were stimulated to release semen using a manual method, 100 with the use of chloroform, and from 75 drones the reproductive organs were dissected for analysis and evaluation. It was found that the principal causes of problems that drones had with ejaculating their semen were anatomical changes or a delay in the development of the mucus glands. It was also found that the method employing chloroform was less efficient in the first phase of eversion of the endophallus, compared with the manual method. The method with the use of chloroform allows the determination of the proportion of drones, which do not evert the endophallus because of poor or delayed development of mucus glands, as well as the proportion of drones which evert the organ, but do not ejaculate semen because of the absence of semen in the seminal vesicles.

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Determination of Water-Soluble Vitamins in Iraqi Honey Bee and Compare with Others Types by High –Performance Liquid Chromatography

Baghdad Science Journal ◽

10.21123/bsj.13.2.447-457 ◽

2016 ◽

Vol 13 (2) ◽

pp. 447-457 ◽

Cited By ~ 1

Author(s):

Baghdad Science Journal

Keyword(s):

Honey Bee ◽

High Performance ◽

High Performance Liquid Chromatographic ◽

Reversed Phase ◽

Uv Spectra ◽

Water Soluble ◽

Good Precision ◽

Concentration Interval ◽

Liquid Chromatographic

High-performance liquid chromatographic methods are used for the determination of water-soluble vitamins with UV-Vis. Detector. A reversed-phase high-performance liquid chromatographic has been developed for determination of water-soluble vitamins. Identification of compounds was achieved by comparing their retention times and UV spectra with those of standards solution. Separation was performed on a C18 column, using an isocratic 30% (v/v) acetonitril in dionozed water as mobile phase at pH 3.5 and flow rate 1.0m/min. The method provides low detection and quantification limits, good linearity in a large concentration interval and good precision. The detection limits ranged from 0.01 to 0.025µg/ml. The accuracy of the method was tested by measuring average recovery values ranged between 94% - 101 %. For standerd solution, and 93%-99% of honey bee samples.

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Relevance of wing morphology in distinguishing and classifying genera and species of Stenogastrinae wasps

Contributions to Zoology ◽

10.1163/18759866-08003003 ◽

2011 ◽

Vol 80 (3) ◽

pp. 191-199 ◽

Cited By ~ 11

Author(s):

David Baracchi ◽

Leonardo Dapporto ◽

Stefano Turillazzi

Keyword(s):

Geometric Morphometrics ◽

Species Identification ◽

Reliable Method ◽

Wing Morphology ◽

Wing Vein ◽

Statistical Assessment ◽

Genus Level ◽

Nesting Material ◽

First Time

The phylogeny of the Stenogastrinae wasps is still under discussion and their systematic incomplete. In the present work we used geometric morphometrics, a technique based on a rigorous statistical assessment of shape, to compare the forewings of fifteen species of Stenogastrinae wasps belonging to four different genera to ascertain whether this approach may be used as a reliable method in the study of the taxonomy of the group. The results show that the wing vein junctions can be diagnostic for both genus and species identification. For the first time in this subfamily, we propose a phylogenetic classification of the species based on wing morphology that largely agrees with the cladistic data available at genus level and reflects the differences among species in terms of nesting material and architecture of their nest.

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