Immobilization of Providencia stuartii Cells in Pumice Stone and Its Application for N-Acetylglucosamine Production

Research background. Shrimp shells contain chitin that can further be processed into N-acetylglucosamine which has been extensively used to treat joint damage. Providencia stuartii isolated form previous research has strong chitinolytic activity and may be utilized in the form of immobilized cells to be used in repeated fermentation. Pumice is a porous and rigid stone that offers superior mechanical strength, making it suitable to be used for immobilization process. Experimental approach. The research used experimental method to conduct the submerged fermentation process with different pumice stone size and pumice stone:growth medium ratio (m/V). The fermentation was carried out for 4 days at 37 C and pH of 7.0. The optimum pumice stone size and pumice stone:growth medium ratio (m/V) were used to determine the optimum fermentation cycle to produce N-acetylglucosamine. Results and conclusions. Pumice stones of 1.0×1.0×1.0 cm and pumice stone:growth medium ratio (m/V) of 1:5 were found to be the optimum conditions which successfully immobilized (89.99±1.65) % cells and produced (331.37±7.34) g/L N-acetylglucosamine. The highest N-acetylglucosamine concentration of (322.97±2.46) g/L was obtained in the first fermentation cycle which then decreased and remained stable throughout the last three cycles of fermentation. Novelty and scientific contribution. P. stuartii was a strong chitinolytic bacteria previously isolated from rotten shrimp shells and was used for the first time in immobilized form to produce N-acetylglucosamine. The findings in this research showed potential use of P. stuartii cells immobilized in pumice stone for continuous production of N-acetylglucosamine using fermentation method.

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Immobilization of Providencia stuartii Cells in Papaya Trunk Wood for N-acetylglucosamine Production from Pennaeus vannamei Shrimp Shells

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v13i2.28011 ◽

2021 ◽

Vol 13 (2) ◽

pp. 208

Author(s):

Yuniwaty Halim ◽

Steven Fausta Tantradjaja ◽

Hardoko Hardoko ◽

Ratna Handayani

Keyword(s):

Experimental Method ◽

Growth Medium ◽

Submerged Fermentation ◽

Natural Compound ◽

Size Ratio ◽

High Concentration ◽

Chitinolytic Bacteria ◽

Fermentation Method ◽

Shrimp Shells ◽

Providencia Stuartii

Highlight Research AbstractChitin is a natural compound found abundantly in shrimp shells. Chitin can be degraded to produce N-acetylglucosamine, which has wide applications in the food and pharmaceutical fields. Fermentation using chitinolytic microorganisms can be used to produce N-acetylglucosamine from shrimp shells’ chitin. One of the strong chitinolytic bacteria that was isolated from previous research was Providencia stuartii. To provide better stability and efficiency in fermentation, P. stuartii cells were immobilized using entrapment method in papaya trunk wood. The aims of this research were to determine the optimum papaya trunk wood size, ratio of papaya trunk wood and growth medium, as well as the optimum fermentation cycle to produce N-acetylglucosamine from P. vannamei shrimp shells using submerged fermentation method. The research used experimental method with treatment of different sizes of papaya trunk wood (1 x 1 x 1 cm3, 1.5 x 1.5 x 1.5 cm3, and 2 x 2 x 2 cm3), different ratio of papaya trunk wood and growth medium (1:10, 1:15 and 1:20), and 4 fermentation cycles. Results showed that papaya trunk wood with size of 1 x 1 x 1 cm3 and ratio (w/v) of 1:10 could immobilize 87.08±2.05% of P. stuartii cells and produce the highest N-acetylglucosamine concentration, which was 238177.78±3153.48 ppm. The highest N-acetylglucosamine production was obtained from first fermentation cycle and decreased over the last three cycles, but still produced high concentration of N-acetylglucosamine. Therefore, it is possible to perform continuous N-acetylglucosamine production from shrimp shells using P. stuartii cells immobilized in papaya trunk wood.

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A DFT study of RuO4 interactions with porous materials: metal–organic frameworks (MOFs) and zeolites

Physical Chemistry Chemical Physics ◽

10.1039/c8cp01950a ◽

2018 ◽

Vol 20 (24) ◽

pp. 16770-16776 ◽

Cited By ~ 14

Author(s):

Siwar Chibani ◽

Michael Badawi ◽

Thierry Loiseau ◽

Christophe Volkringer ◽

Laurent Cantrel ◽

...

Keyword(s):

Hydrogen Bonds ◽

Porous Materials ◽

Metal Organic Frameworks ◽

Dft Study ◽

Hydrated Form ◽

Metal Organic ◽

First Time ◽

Potential Use ◽

Multiple Hydrogen Bonds

The potential use of zeolite and MOF materials for the capture of RuO4 has been investigated for the first time. A hydrated form of HKUST-1 could be a promising sorbent due to its ability to form multiple hydrogen bonds.

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Semicontinuous and Continuous Production of Citric Acid with Immobilized Cells of Aspergillus niger

Zeitschrift für Naturforschung C ◽

10.1515/znc-1987-0413 ◽

1987 ◽

Vol 42 (4) ◽

pp. 408-413 ◽

Cited By ~ 16

Author(s):

H. Eikmeier ◽

H. J. Rehm

Keyword(s):

Citric Acid ◽

Aspergillus Niger ◽

Immobilized Cells ◽

Continuous Production ◽

Continuous Process ◽

Batch Experiments ◽

Medium Exchange ◽

Free Cells ◽

Semicontinuous Cultivation ◽

Ca Alginate

Abstract The citric acid excretion of Ca-alginate-immobilized cells of Aspergillus niger in batch culture decreased with a half-time of approximately 19 days. Reactivation of the biocatalysts by regeneration in growth medium was possible, but it was followed by a submerged sporulation of the fungus, and medium was highly contaminated with free cells. Citric acid production could better be prolonged by semicontinuous cultivation with medium exchange every 7 or 14 days, respectively. After 32 days the remaining activity in semicontinuous culture was 1.4-fold higher than in comparable batch experiments. Similar improvements were obtained with a continuous process at a dilution rate of 0.125 v/v · d, whereby medium efflux completely free of detaching mycelia.

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Removal of dimethachlon from soils using immobilized cells and enzymes of a novel potential degrader Providencia stuartii JD

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2019.04.089 ◽

2019 ◽

Vol 378 ◽

pp. 120606 ◽

Cited By ~ 5

Author(s):

Cheng Zhang ◽

Xinglu Pan ◽

Xiaomao Wu ◽

Fengshou Dong ◽

Xingang Liu ◽

...

Keyword(s):

Immobilized Cells ◽

Providencia Stuartii

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Rhipicephalus turanicus, a new vector of Hepatozoon canis

Parasitology ◽

10.1017/s003118201600250x ◽

2016 ◽

Vol 144 (6) ◽

pp. 730-737 ◽

Cited By ~ 19

Author(s):

ALESSIO GIANNELLI ◽

RICCARDO PAOLO LIA ◽

GIADA ANNOSCIA ◽

CANIO BUONAVOGLIA ◽

ELEONORA LORUSSO ◽

...

Keyword(s):

Southern Italy ◽

Rhipicephalus Sanguineus ◽

Hepatozoon Canis ◽

Time Data ◽

Tick Vector ◽

First Time ◽

Potential Use ◽

Potential Tool

SUMMARYThe distribution of Hepatozoon canis mainly encompasses areas where its main tick vector, Rhipicephalus sanguineus sensu lato, is present. However, the detection of this pathogen in dogs, foxes and golden jackals well outside the areas inhabited by this tick species reinforced the hypothesis that additional ixodids are involved in the life cycle and transmission of this protozoon. The present study provides, for the first time, data supporting the sporogonic development of H. canis in specimens of Rhipicephalus turanicus collected from a naturally infected fox from southern Italy. The epidemiological role of R. turanicus as a vector of H. canis is discussed, along with information on the potential use of cell cultures for the experimental infection with H. canis sporozoites. The in vitro infection of canine leucocytes by sporozoites from ticks is proposed as a potential tool for future in-depth studies on the biology of H. canis.

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Germ cell survival and differentiation after xenotransplantation of testis tissue from three endangered species: Iberian lynx (Lynx pardinus), Cuvier's gazelle (Gazella cuvieri) and Mohor gazelle (G. dama mhorr)

Reproduction Fertility and Development ◽

10.1071/rd12411 ◽

2014 ◽

Vol 26 (6) ◽

pp. 817 ◽

Cited By ~ 8

Author(s):

Lucía Arregui ◽

Ina Dobrinski ◽

Eduardo R. S. Roldan

Keyword(s):

Endangered Species ◽

Biological Diversity ◽

Testicular Tissue ◽

Seminiferous Tubules ◽

Lynx Lynx ◽

Lynx Pardinus ◽

Reproductive Techniques ◽

First Time ◽

Potential Use ◽

Testis Tissue

The use of assisted reproductive techniques for endangered species is a major goal for conservation. One of these techniques, testis tissue xenografting, allows for the development of spermatozoa from animals that die before reaching sexual maturity. To assess the potential use of this technique with endangered species, testis tissue from six Iberian lynxes (one fetus, two perinatal cubs, two 6-month-old and one 2-year-old lynx), two Cuvier’s gazelle fetuses and one 8-month-old Mohor gazelle were transplanted ectopically into nude mice. Tissue from the lynx fetus, perinatal cubs and 2-year-old donors degenerated, whereas spermatogonia were present in 15% of seminiferous tubules more than 70 weeks after grafting in transplanted testis tissue from 6-month-old donors. Seminal vesicle weights (indicative of testosterone production) increased over time in mice transplanted with tissue from 6-month-old lynxes. Progression of spermatogenesis was observed in xenografts from gazelles and was donor age dependent. Tissue from Cuvier’s gazelle fetuses contained spermatocytes 40 weeks after grafting. Finally, round spermatids were found 28 weeks after transplantation in grafts from the 8-month-old Mohor gazelle. This is the first time that xenotransplantation of testicular tissue has been performed with an endangered felid and the first successful xenotransplantation in an endangered species. Our results open important options for the preservation of biological diversity.

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