Tracking of powdery mildew and leaf rust resistance genes in Triticum boeoticum and T. urartu, wild relatives of common wheat

Wild Triticum and Aegilops species are increasingly used in wheat breeding programmes around the world as donors of genes conferring resistance to biotic and abiotic stresses, as well as of genes that contribute to the improvement of grain quality. In the present study, thirty-nine accessions of diploid species with the A genome (Triticum boeoticum and T. urartu) were evaluated for the presence of the genes conferring resistance to powdery mildew (Blumeria graminis) and leaf rust (Puccinia recondita) using both inoculation tests and sequence tagged sites (STS) marker analyses in order to find correspondence between STS markers and resistance as a trait. The most resistant entries were T. boeoticum accessions. All the marked Lr and Pm resistance genes (Pm1, Pm2, Pm3, Lr10, Lr47, Lr25 and Lr28) were identified in the check T. aestivum cultivar Bezostaya 1. The resistance to powdery mildew in the material studied was conferred by the combination of the Pm1 gene with either Pm2 or Pm3. The Pm1 and Pm3 markers appeared to be suitable for tracking these powdery mildew resistance genes, while the Pm2 gene marker cannot be considered as usable in various genetically different wheat accessions. The presence of the genes Lr25, Lr28 and Lr47 seems to be particularly useful for obtaining leaf rust resistance in T. boeoticum and T. urartu species.  

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Effectiveness of major resistance genes and identification of new sources for disease resistance in wheat

Acta Agronomica Hungarica ◽

10.1556/aagr.59.2011.3.7 ◽

2011 ◽

Vol 59 (3) ◽

pp. 241-248 ◽

Cited By ~ 2

Author(s):

G. Vida ◽

M. Cséplő ◽

G. Gulyás ◽

I. Karsai ◽

T. Kiss ◽

...

Keyword(s):

Disease Resistance ◽

Powdery Mildew ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Genetic Material ◽

Leaf Rust Resistance ◽

Wheat Varieties ◽

Major Resistance Genes ◽

Greenhouse Tests

Among the factors which determine yield reliability an important role is played by disease resistance. One of the breeding aims in the Martonvásár institute is to develop wheat varieties with resistance to major diseases. The winter wheat varieties bred in Martonvásár are examined in artificially inoculated nurseries and greenhouses for resistance to economically important pathogens. The effectiveness of designated genes for resistance to powdery mildew and leaf rust has been monitored over a period of several decades. None of the designated major resistance genes examined in greenhouse tests is able to provide complete resistance to powdery mildew; however, a number of leaf rust resistance genes provide full protection against pathogen attack (Lr9, Lr19, Lr24, Lr25, Lr28 and Lr35). In the course of marker-assisted selection, efficient resistance genes (Lr9, Lr24, Lr25 and Lr29) have been incorporated into Martonvásár wheat varieties. The presence of Lr1, Lr10, Lr26, Lr34 and Lr37 in the Martonvásár gene pool was identified using molecular markers. New sources carrying alien genetic material have been tested for powdery mildew and leaf rust resistance. Valuable Fusarium head blight resistance sources have been identified in populations of old Hungarian wheat varieties. Species causing leaf spots (Pyrenophora tritici-repentis, Septoria tritici and Stagonospora nodorum) have gradually become more frequent over the last two decades. Tests on the resistance of the host plant were begun in Martonvásár four years ago and regular greenhouse tests on seedlings have also been initiated.

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Mapping of powdery mildew and leaf rust resistance genes on the wheat-rye translocated chromosome T1BL.1RS using molecular and biochemical markers

Plant Breeding ◽

10.1046/j.1439-0523.2000.00444.x ◽

2000 ◽

Vol 119 (1) ◽

pp. 87-89 ◽

Cited By ~ 22

Author(s):

S. L. K. Hsam ◽

V. Mohler ◽

L. Hartl ◽

G. Wenzel ◽

F. J. Zeller

Keyword(s):

Powdery Mildew ◽

Leaf Rust ◽

Resistance Genes ◽

Biochemical Markers ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

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Evidence of allelism between genes Pm8 and Pm17 and chromosomal location of powdery mildew and leaf rust resistance genes in the common wheat cultivar'Amigo'

Plant Breeding ◽

10.1111/j.1439-0523.1997.tb02164.x ◽

1997 ◽

Vol 116 (2) ◽

pp. 119-122 ◽

Cited By ~ 54

Author(s):

S. L. K. Hsam ◽

F. J. Zeller

Keyword(s):

Powdery Mildew ◽

Leaf Rust ◽

Common Wheat ◽

Resistance Genes ◽

Rust Resistance ◽

Chromosomal Location ◽

Leaf Rust Resistance ◽

The Common ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

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Genetic Analysis of Leaf Rust Resistance in Six Durum Wheat Genotypes

Phytopathology ◽

10.1094/phyto-03-14-0065-r ◽

2014 ◽

Vol 104 (12) ◽

pp. 1322-1328 ◽

Cited By ~ 8

Author(s):

Alexander Loladze ◽

Dhouha Kthiri ◽

Curtis Pozniak ◽

Karim Ammar

Keyword(s):

Durum Wheat ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Durable Resistance ◽

Puccinia Triticina ◽

Leaf Rust Resistance ◽

Adult Plant ◽

Seedling Resistance ◽

Allelism Tests

Leaf rust, caused by Puccinia triticina, is one of the main fungal diseases limiting durum wheat production. This study aimed to characterize previously undescribed genes for leaf rust resistance in durum wheat. Six different resistant durum genotypes were crossed to two susceptible International Maize and Wheat Improvement Center (CIMMYT) lines and the resulting F1, F2, and F3 progenies were evaluated for leaf rust reactions in the field and under greenhouse conditions. In addition, allelism tests were conducted. The results of the study indicated that most genotypes carried single effective dominant or recessive seedling resistance genes; the only exception to this was genotype Gaza, which carried one adult plant and one seedling resistance gene. In addition, it was concluded that the resistance genes identified in the current study were neither allelic to LrCamayo or Lr61, nor were they related to Lr3 or Lr14a, the genes that already are either ineffective or are considered to be vulnerable for breeding purposes. A complicated allelic or linkage relationship between the identified genes is discussed. The results of the study will be useful for breeding for durable resistance by creating polygenic complexes.

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Structural and functional analysis of genes with potential involvement in resistance to coffee leaf rust: a functional marker based approach

10.1101/763425 ◽

2019 ◽

Author(s):

Geleta Dugassa Barka ◽

Eveline Teixeira Caixeta ◽

Sávio Siqueira Ferreira ◽

Laércio Zambolim

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Function Analysis ◽

Bac Library ◽

Leaf Rust Resistance ◽

Molecular Data ◽

Coffee Leaf Rust ◽

Rust Resistance Genes

AbstractPhysiology-based differentiation of SH genes and Hemileia vastatrix races is the principal method employed for the characterization of coffee leaf rust resistance. Based on the gene-for-gene theory, nine major rust resistance genes (SH1-9) have been proposed. However, these genes have not been characterized at the molecular level. Consequently, the lack of molecular data regarding rust resistance genes or candidates is a major bottleneck in coffee breeding. To address this issue, we screened a BAC library with resistance gene analogs (RGAs), identified RGAs, characterized and explored for any SH related candidate genes. Herein, we report the identification and characterization of a gene (gene 11), which shares conserved sequences with other SH genes and displays a characteristic polymorphic allele conferring different resistance phenotypes. Furthermore, comparative analysis of the two RGAs belonging to CC-NBS-LRR revealed more intense diversifying selection in tomato and grape genomes than in coffee. For the first time, the present study has unveiled novel insights into the molecular nature of the SH genes, thereby opening new avenues for coffee rust resistance molecular breeding. The characterized candidate RGA is of particular importance for further biological function analysis in coffee.

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Development of barley introgression lines carrying the leaf rust resistance genes Rph1 to Rph15

Crop Science ◽

10.1002/csc2.20057 ◽

2020 ◽

Vol 60 (1) ◽

pp. 282-302 ◽

Cited By ~ 1

Author(s):

Matthew J. Martin ◽

Oswaldo Chicaiza ◽

Juan C. Caffarel ◽

Ahmad H. Sallam ◽

Arnis Druka ◽

...

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Introgression Lines ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

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Identification of Leaf Rust Resistance Genes in Selected Wheat Cultivars and Development of Multiplex PCR

Open Life Sciences ◽

10.1515/biol-2019-0036 ◽

2019 ◽

Vol 14 (1) ◽

pp. 327-334 ◽

Cited By ~ 2

Author(s):

Agnieszka Tomkowiak ◽

Roksana Skowrońska ◽

Alicja Buda ◽

Danuta Kurasiak-Popowska ◽

Jerzy Nawracała ◽

...

Keyword(s):

Multiplex Pcr ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Field Resistance ◽

Wheat Cultivars ◽

Pcr Conditions ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

AbstractTen leading wheat cultivars originating from the Plant Breeding and Acclimatization Institute (IHAR) - National Research Institute (Poland) and the Department of Gene Bank (Czech Republic) were used to establish a field experiment in 2017 and 2018 at the Dłoń Experimental Farm. The analyzed wheat genotypes were characterized by diversified field resistance to leaf rust. Jubilatka, Thatcher and Sparta were the most resistant cultivars in field conditions in both 2017 and 2018. The aim of the work was to identify the Lr11, L13, Lr16 and Lr26 genes encoding resistance to leaf rust using molecular SSR markers (wmc24, wmc261, Xgwm630, Xwmc764 and P6M12) and to develop multiplex PCR conditions to accelerate identification of these genes. Markers of three leaf rust resistance genes have been identified simultaneously in these cultivars. Jubilatka, Thatcher and Sparta cultivars may serve as a good source of the analyzed leaf rust resistance genes. In addition, multiplex PCR conditions have been developed for the simultaneous identification of the Lr11 and Lr16 and Lr11 and Lr26 gene pairs.

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Identification and Molecular Characterization of Leaf Rust Resistance Gene Lr14a in Durum Wheat

Plant Disease ◽

10.1094/pdis-92-3-0469 ◽

2008 ◽

Vol 92 (3) ◽

pp. 469-473 ◽

Cited By ~ 43

Author(s):

S. A. Herrera-Foessel ◽

R. P. Singh ◽

J. Huerta-Espino ◽

H. M. William ◽

V. Garcia ◽

...

Keyword(s):

Durum Wheat ◽

Leaf Rust ◽

Resistance Gene ◽

Ssr Markers ◽

Common Wheat ◽

Resistance Genes ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Rust Resistance Gene ◽

Leaf Rust Resistance Gene

Leaf rust, caused by Puccinia triticina, is an important disease of durum wheat (Triticum turgidum subsp. durum) and only a few designated resistance genes are known to occur in this crop. A dominant leaf rust resistance gene in the Chilean durum cv. Llareta INIA was mapped to chromosome arm 7BL through bulked segregant analysis using the amplified fragment length polymorphism (AFLP) technique, and by mapping three polymorphic markers in the common wheat (T. aestivum) International Triticeae Mapping Initiative population. Several simple sequence repeat (SSR) markers, including Xgwm344-7B and Xgwm146-7B, were associated with the leaf rust resistance gene. Resistance response and chromosomal position indicated that this gene is likely to be Lr14a. The SSR markers Xgwm344-7B and Xgwm146-7B and one AFLP marker also differentiated common wheat cv. Thatcher from the near-isogenic line with Lr14a, as well as durum ‘Altar C84’ from durum wheat with Lr14a. This is the first report of the presence of Lr14a in durum wheat, although the gene originally was transferred from emmer wheat ‘Yaroslav’ to common wheat. Lr14a is also present in CIMMYT-derived durum ‘Somateria’ and effective against Mexican and other P. triticina races of durum origin. Lr14a should be deployed in combination with other effective leaf rust resistance genes to prolong its effectiveness in durum wheat.

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