scholarly journals Platelet-rich plasma, bone marrow and chitosan in minimally invasive plate osteosynthesis of canine tibia fractures – a randomized study

2019 ◽  
Vol 64 (No. 7) ◽  
pp. 309-316 ◽  
Author(s):  
FGF Filgueira ◽  
BW Minto ◽  
DG Chung ◽  
TC Prada ◽  
NM Rosa-Ballaben ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Minimally Invasive ◽  
Plate Osteosynthesis ◽  
Platelet Rich Plasma ◽  
Control Group ◽  
Minimally Invasive Plate Osteosynthesis ◽  
Bone Marrow Concentrate ◽  
Bone Consolidation ◽  
Post Surgery ◽  
Significant Difference

The goal of this study was to analyse the effects of percutaneous application of platelet rich plasma (PRP), autologous bone marrow concentrate (BM) and chitosan gel (CHI) on bone consolidation following minimally invasive plate osteosynthesis (MIPO) of the fractures of the tibia in dogs. Client-owned dogs (n = 30) with tibial fracture were divided into four treatment groups – Group 1 (control), Group 2 (BM), Group 3 (PRP) and Group 4 (CHI). The biomaterial specific to each group was injected at the fracture site immediately after the MIPO procedure. Serial radiographs were used to determine the fracture line and the development of periosteal callus immediately after surgery and at 15, 30, 60, 90 and 120 days post-surgery. There was no significant difference (P > 0.05) in the degree of oedema or grade of lameness between the groups. Grade 4 (minimum) or 5 lameness (absent) was observed in 70% of animals from all groups at 15 days post-surgery. The biomaterials PRP, BM and CHI combined with MIPO contribute to bone consolidation of tibial fractures in dogs and do not cause adverse reactions or fracture complications. Bone marrow concentrate results in shorter bone consolidation time.

scholarly journals Effect of Oral Losartan on Orthobiologics: Implications for Platelet-Rich Plasma and Bone Marrow Concentrate—A Rabbit Study

2020 ◽  
Vol 21 (19) ◽  
pp. 7374
Author(s):  
Gilberto Y. Nakama ◽  
Sabrina Gonzalez ◽  
Polina Matre ◽  
Xiaodong Mu ◽  
Kaitlyn E. Whitney ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteochondral Defect ◽  
Platelet Rich Plasma ◽  
Control Group ◽  
Bone Marrow Concentrate ◽  
Significant Difference ◽  
Losartan Group ◽  
Group 2 ◽  
And Control ◽  
Tgf Β1

Recent efforts have focused on customizing orthobiologics, such as platelet-rich plasma (PRP) and bone marrow concentrate (BMC), to improve tissue repair. We hypothesized that oral losartan (a TGF-β1 blocker with anti-fibrotic properties) could decrease TGF-β1 levels in leukocyte-poor PRP (LP-PRP) and fibrocytes in BMC. Ten rabbits were randomized into two groups (N = 5/group): osteochondral defect + microfracture (control, group 1) and osteochondral defect + microfracture + losartan (losartan, group 2). For group 2, a dose of 10mg/kg/day of losartan was administrated orally for 12 weeks post-operatively. After 12 weeks, whole blood (WB) and bone marrow aspirate (BMA) samples were collected to process LP-PRP and BMC. TGF-β1 concentrations were measured in WB and LP-PRP with multiplex immunoassay. BMC cell populations were analyzed by flow cytometry with CD31, CD44, CD45, CD34, CD146 and CD90 antibodies. There was no significant difference in TGF-β1 levels between the losartan and control group in WB or LP-PRP. In BMC, the percentage of CD31+ cells (endothelial cells) in the losartan group was significantly higher than the control group (p = 0.008), while the percentage of CD45+ cells (hematopoietic cells-fibrocytes) in the losartan group was significantly lower than the control group (p = 0.03).

Retrospective comparison between minimally invasive plate osteosynthesis and open plating for tibial fractures in dogs

2012 ◽  
Vol 25 (05) ◽  
pp. 410-417 ◽  
Author(s):  
A. Boero Baroncelli ◽  
B. Peirone ◽  
M. D. Winter ◽  
D. J. Reese ◽  
A. Pozzi
Keyword(s):  
Minimally Invasive ◽  
Plate Osteosynthesis ◽  
Major Complication ◽  
Complete Healing ◽  
Tibial Fractures ◽  
Minimally Invasive Plate Osteosynthesis ◽  
Fracture Length ◽  
Plate Length ◽  
Working Length ◽  
Significant Difference

SummaryObjectives: To compare fracture healing in diaphyseal tibial fractures stabilized using either minimally invasive plate osteosynthesis (MIPO) or open reduction and internal fixation (ORIF).Methods: Dogs in each group were matched for type of fracture, age and body weight. Stage of healing was measured blindly every four weeks postoperatively until complete healing. Outcome variables including fracture length, plate length, plate bridging ratio, plate working length, healing grading, and fracture reduction were compared between groups using the Mann-Whitney test. Significant difference was set at p <0.05.Results: Based on the definition of clinical union, at 30 days five out of eight dogs managed with MIPO had healed, while two of the eight of dogs managed with ORIF had healed. We did not find any significant differences in the other outcome measures. No complications were reported in the MIPO group whereas one major complication was reported in the ORIF group.Clinical significance: All dogs treated by MIPO healed rapidly without any complications, nevertheless the difference in radiographic healing between the two groups was not significant.

Minimally Invasive Plate Osteosynthesis Using Fracture Reduction Under the Plate without Intraoperative Fluoroscopy to Stabilize Diaphyseal Fractures of the Tibia and Femur in Dogs and Cats

2019 ◽  
Vol 32 (06) ◽  
pp. 475-482 ◽  
Author(s):  
Julien Cabassu
Keyword(s):  
Postoperative Complications ◽  
Minimally Invasive ◽  
Tibial Plateau ◽  
Plate Osteosynthesis ◽  
Femoral Fractures ◽  
Fracture Reduction ◽  
Minimally Invasive Plate Osteosynthesis ◽  
Tibial Torsion ◽  
Cortical Screw ◽  
Significant Difference

Objective The aim of this study was to prospectively evaluate postoperative alignment when using fracture reduction under the plate (FRUP) during a minimally invasive plate osteosynthesis in tibial and femoral fractures, without intraoperative imaging, and report immediate postoperative complications. Materials and Methods After precise plate contouring and preoperative planning, FRUP was obtained with one cortical screw per fragment. Fractures were stabilized with a plate or plate rod. Tibial/femoral lengths, tibial plateau angles, mechanical medial proximal and distal tibial angles, anatomical lateral distal femoral angles, femoral curvatum and neck anteversion were evaluated on postoperative radiographs and contralateral bone. Tibial torsion was evaluated visually. Paired t-test were used to compare data. Immediate postoperative complications were recorded. Results Twenty-one tibial and 20 femoral fractures were stabilized (14 plate rod cases). Mean postoperative operated tibial length was 1.4% shorter (p = 0.001). Mean postoperative operated femoral length was 2% shorter (p = 0.04). Mean operated tibial plateau angle was 1.1° lower (p = 0.02). No difference in tibial torsion was noticed. No significant difference in mechanical medial proximal tibial angle, mechanical medial distal tibial angle, anatomical lateral distal femoral angle and femoral neck anteversion was observed. Mean operated femoral curvatum angle was 5.6° less (p = 0.01). Five cases (3 plate rod cases) required an immediate revision. Clinical Significance Minimally invasive plate osteosynthesis with FRUP leads to acceptable postoperative alignment. Correct pin placement should be evaluated.

scholarly journals Long Volar Plating for Metadiaphyseal Fractures of Distal Radius: Study Comparing Minimally Invasive Plate Osteosynthesis versus Conventional Approach

2017 ◽  
Vol 06 (03) ◽  
pp. 227-234 ◽  
Author(s):  
Emilie Pire ◽  
Juan Hidalgo Diaz ◽  
Santiago Salazar Botero ◽  
Sybille Facca ◽  
Philippe Liverneaux
Keyword(s):  
Minimally Invasive ◽  
Distal Radius ◽  
Plate Osteosynthesis ◽  
Conventional Approach ◽  
Minimally Invasive Plate Osteosynthesis ◽  
Dash Score ◽  
Volar Plating ◽  
Significant Difference ◽  
Group 2 ◽  
Group 1

Background Minimally invasive plate osteosynthesis (MIPO) has been used in wrist surgery for several years. The purpose of this retrospective study was to compare clinical and radiologic outcomes of MIPO technique with those of a conventional approach in the treatment of metadiaphyseal distal radius fracture by long volar plating. Materials and Methods Our series consisted of 32 fractures in 31 patients, mean age 63.9 years, including 16 men and 15 women. MIPO technique was used in 15 wrists (group 1) and conventional approach (> 60 mm of skin incision) in 17 wrists (group 2). In group 1, a long volar plate was inserted under pronator quadratus through a 15- to 30-mm distal incision then fixed to the epiphysis of the distal radius. Then, through a 15- to 30-mm proximal incision, the plate was fixed to the diaphysis of the radius, thus reducing the fracture. Results In group 1, mean distal incision size was 23.5 and 16.9 mm for proximal one. Mean total scar size (sum of both distal and proximal incisions) was 40.0 mm in group 1 and 84.1 mm in group 2. Mean tourniquet time was 58.4 minutes in group 1 and 68.9 minutes in group 2. At latest follow-up, no significant difference was noted in both the groups concerning pain, quick-DASH score, grip strength, ROM, and radiologic data. One extensor pollicis longus rupture treated by tendon transfer was done in group 1. Conclusion The MIPO technique for metadiaphyseal fractures of the distal radius by long volar plating has cosmetic and economic advantages compared with the conventional approach. Conversion to conventional approach is possible at any time in case of technical difficulties. Level of Evidence III.

scholarly journals Bridge plating for simple tibial fractures treated by minimally invasive plate osteosynthesis

2021 ◽  
Vol 73 (3) ◽  
pp. 589-597
Author(s):  
B.M. Alcântara ◽  
B.W. Minto ◽  
G.G. Franco ◽  
D.V.F. Lucena ◽  
L.G.G.G. Dias
Keyword(s):  
Minimally Invasive ◽  
Plate Osteosynthesis ◽  
Frontal Plane ◽  
Tibial Fractures ◽  
Minimally Invasive Plate Osteosynthesis ◽  
Complication Rates ◽  
Bridge Plate ◽  
Working Length ◽  
Significant Difference ◽  
Bridge Plating

ABSTRACT This study aimed to evaluate the effectiveness of bridge plating of simple tibial fractures in dogs by minimally invasive plate osteosynthesis (MIPO). Medical and radiographic records of twenty-nine dogs with simple tibial fractures that underwent bridge fixation by MIPO were retrospectively evaluated. The clinical outcome was classified considering the presence of lameness at the end of the treatment. The tibial mechanical joint angles were measured and compared with the values described in the literature. Additionally, fragment apposition and implant disposition were evaluated. Based on the modified Radiographic Union Scale for Tibial fractures, the moment of clinical union was determined. Clinically, at the end of treatment, only one patient presented lameness at a trot. While there was no significant difference between the bone alignment in the frontal plane values and the values described in the literature (P>0.05), the caudal proximal tibial angle was significantly higher (P=0.001). The median fragment apposition was considered acceptable. The average bridge plate ratio, plate working length, and plate screw density were 0.8, 0.57, and 0.48, respectively. The median time to clinical union was 30 days. Bridge plating in simple tibial fractures resulted in fast healing and low complication rates.

scholarly journals Assessing the Potential of Bone Marrow Concentrate for Cartilage Repair and Regeneration in Animal Models: A Systemic Review

2021 ◽  
Vol 50 (6) ◽  
pp. 1727-1744
Author(s):  
Nabillah Abd Radzak ◽  
Malliga Raman Murali ◽  
Tunku Kamarul Tunku Kamarul
Keyword(s):  
Bone Marrow ◽  
Animal Models ◽  
Cartilage Repair ◽  
Cartilage Defect ◽  
Transforming Growth Factor ◽  
Platelet Rich Plasma ◽  
Stem Cell Differentiation ◽  
Systemic Review ◽  
Bone Marrow Concentrate ◽  
Significant Difference

Bone marrow concentrate (BMC) has been emerging as a promising regenerative source to accelerate cartilage regeneration in cartilage injuries and osteoarthritis. Though the number of stem cells in BMC is limited, BMC is rich in growth factors that promote stem cell differentiation and tissue regeneration. Despite of multiple reports available on the use of BMC for cartilage repair in humans and its use in clinical settings, only limited number of pre-clinical proof of concept studies have been reported in animal models. Hence, a systematic review focusing on the potential of BMCfor the treatment of cartilage defect in animal models has been conducted. The systematic search of literature using three popular databases, ISI Web of Knowledge, PubMed and Scopus, were conducted without year restriction. Fifteen (n = 15) studies were found appropriate and included in this review. All of the included studies were of different animal models with cartilage defect. 13 out of 15 studies reported that the usage of BMC gave the best outcome compared to other treatment methods. Most of the findings provided good scoring on the tissue repair and the histological outcome. However, most of the BMC group outcomes did not give a significant difference when compared with other interventions such as the addition of platelet rich plasma, erythropoietin, hyaluronic acid, transforming growth factor, autologous tissue implant, genetic modification or scaffoldings. In conclusion, the published studies do suggest that BMC could provide a better cartilage repair. However, more preclinical studies are required to provide definitive conclusions.

Danger zone for screw placement in minimally invasive plate osteosynthesis (MIPO) of diaphyseal fractures of forearm: a cadaveric study

2021 ◽  
Author(s):  
Apipop Kritsaneephaiboon ◽  
Watit Wuttimanop ◽  
Surasak Jitprapaikulsarn ◽  
Pornpanit Dissaneewate ◽  
Chulin Chewakidakarn ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Plate Osteosynthesis ◽  
Cadaveric Study ◽  
Screw Placement ◽  
Minimally Invasive Plate Osteosynthesis ◽  
Danger Zone ◽  
Diaphyseal Fractures

Evaluation of genotoxicity of pan masala employing chromosomal aberration and micronucleus assay in bone marrow cells of the mice

2009 ◽  
Vol 25 (7) ◽  
pp. 467-471 ◽  
Author(s):  
BN Mojidra ◽  
K. Archana ◽  
AK Gautam ◽  
Y. Verma ◽  
BC Lakkad ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberration ◽  
Chromosomal Aberration ◽  
Bone Marrow Cells ◽  
Micronucleus Assay ◽  
Control Group ◽  
Genotoxic Potential ◽  
Polychromatic Erythrocytes ◽  
Significant Difference ◽  
Marrow Cells

Pan masala is commonly consumed in south-east Asian and other oriental countries as an alternate of tobacco chewing and smoking. Genotoxic potential of pan masala (pan masala plain and pan masala with tobacco known as gutkha) was evaluated employing chromosome aberration (CA) and micronucleus (MN) assay in vivo. Animals were exposed to three different doses (0.5%, 1.5% and 3%) of pan masala plain (PMP) and gutkha (PMT) through feed for a period of 6 months and micronucleus and chromosomal aberrations were studied in the bone marrow cells. Induction of mean micronuclei in polychromatic erythrocytes (MNPCE) and normochromatic erythrocyte (MNNCE) was higher in both types of pan masala treated groups with respect to control group. Both pan masala plain and gutkha treatment significantly induced the frequency of MNPCE and MNNCE in the bone marrow cells, indicating the genotoxic potential. Furthermore, slight decline in the ratio of polychromatic erythrocytes to normochromatic erythrocytes was also noticed, suggesting the cytotoxic potential even though the ratio was statistically non significant. A dose-dependent, significant increase in chromosome aberration was observed in both types of pan masala treated mice with respect to control. However, no significant difference in micronucleus and chromosomal aberration induction was noticed between two types of pan masala exposed (PMP and PMT) groups. Results suggest that both types of pan masala, i.e. plain and gutkha, have genotoxic potential.

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