scholarly journals Prune cv. Jojo resistance to different strains of Plum pox virus

2010 ◽  
Vol 41 (No. 2) ◽  
pp. 47-51 ◽  
Author(s):  
J. Polák ◽  
J. Pívalová ◽  
J. Svoboda
Keyword(s):  
Czech Republic ◽  
Hypersensitive Reaction ◽  
Plum Pox Virus ◽  
Prunus Domestica ◽  
The Czech Republic ◽  
Different Strains

Trees of prune (<i>Prunus domestica</i> L.), cv. Jojo, were inoculated by chip budding with three different strains of PPV isolated from European plum in the Czech Republic. These isolates included Plum pox virus M strain (PPV-M), <i>Plum pox virus</i> D strain (PPV-D) and a PPV-recombinant both strains (PPV-Rec). The results of the evaluation of the inoculated trees over 2 years are presented. Trees of plum cv. Jojo behaven differently to infection with the three PPV strains. A strong hypersensitive reaction appeared a year after inoculation with PPV-M and PPV-Rec strains, although not all inoculated tree died. PPV must have been present in the tissue of cv. Jojo because the virus was transferred to the rootstock St. Julien. Plants of the rootstock became systemically infected with the PPV-M and PPV-Rec strains, showing severe PPV symptoms. The presence of PPV was proved by ELISA in leaves of rootstock St. Julien, but not in leaves of cv. Jojo. Inoculation with strain PPV-D resulted in partial hypersensitive reaction of plants of cv. Jojo, but after initial stunting and partial death of shoots recovering of plants was observed.

scholarly journals Reaction of transgenic plum cv. HoneySweet to the Plum pox virus after a severe infection of Monilinia sp. – short communication

2018 ◽  
Vol 55 (No. 1) ◽  
pp. 8-10 ◽  
Author(s):  
Polák Jaroslav ◽  
Neubauerová Tereza ◽  
Komínek Petr ◽  
Kundu Jiban Kumar
Keyword(s):  
Czech Republic ◽  
Short Communication ◽  
Severe Infection ◽  
Pcr Detection ◽  
Plum Pox Virus ◽  
Prunus Domestica ◽  
Rt Pcr ◽  
The Czech Republic ◽  
Das Elisa ◽  
Year 2013

Resistance to Plum pox virus (PPV) in transgenic Prunus domestica L., clone C5 (cv. HoneySweet) was evaluated in a regulated field in the Czech Republic for fifteen years (2002–2016). PPV mild symptoms appeared in C5 trees only in several leaves situated close to the point of inoculum grafting up to 2010. No symptoms of PPV were observed in the years 2011–2013 and results of ELISA and RT-PCR detection tests were negative. In the twelfth year (2013), there was a severe unusual natural attack of plum trees by Monilinia sp. This Monilinia sp. attack occurred only one time – in 2013. There was no Monilinia sp. infection in 2002–2012 and in 2014–2016. Mild PPV symptoms reappeared in several leaves of transgenic plum trees in the next two years (2014–2015) and the presence of PPV was proved by DAS-ELISA and confirmed by RT-PCR.

scholarly journals Influence of climate changes in the Czech Republic on the distribution of plant viruses and phytoplasmas originally from the mediterranean subtropical region

2010 ◽  
Vol 45 (Special Issue) ◽  
pp. S20-S26 ◽  
Author(s):  
J. Polák
Keyword(s):  
Czech Republic ◽  
Climate Changes ◽  
Plant Pathogens ◽  
Zucchini Yellow Mosaic Virus ◽  
Plant Viruses ◽  
Northern Italy ◽  
Yellow Mosaic Virus ◽  
Plum Pox Virus ◽  
The Czech Republic ◽  
Peach Trees

Results of research aiming at monitoring of climate changes impact on plant pathogens distribution such as <i>Zucchini yellow mosaic virus</i> (ZYMV), quarantine <i>Plum pox virus</i> (PPV) and quarantine phytoplasma European stone fruit yellows (ESFY) are presented here. ZYMV has spread from Northern Italy across Austria up to Central Moravia and Bohemia. PPV has been continuously spreading from the lowlands of Central Bohemia and Moravia up to plains. Later, from the sixties and seventies of the last century, due to climate warming and human activities the virus quickly spread to uplands, foothills and mountains of the Czech Republic. Phytoplasma ESFY was spreading in a manner similar to ZYMV in the eighties of the twentieth century from Northern Italy and currently is affecting mainly apricot and peach trees in Southern Moravia.

scholarly journals First Report of Apricot pseudo-chlorotic leaf spot virus Infecting Plum (Prunus domestica) in the Czech Republic

Plant Disease ◽  
2012 ◽  
Vol 96 (3) ◽  
pp. 461-461 ◽  
Author(s):  
D. Šafářová ◽  
M. Navrátil ◽  
C. Faure ◽  
T. Candresse ◽  
A. Marais
Keyword(s):  
Czech Republic ◽  
Amino Acid ◽  
Leaf Spot ◽  
Fruit Trees ◽  
Stone Fruit ◽  
Prunus Domestica ◽  
Rt Pcr ◽  
Spot Virus ◽  
The Czech Republic ◽  
Chlorotic Leaf

Apricot pseudo-chlorotic leaf spot virus (APCLSV) is a novel, still poorly known Trichovirus in the family Betaflexiviridae. It is most closely related to Apple chlorotic leaf spot virus (ACLSV) (2,4) and infects stone fruit trees of the Prunus genus. Its presence has so far been detected in apricot, plum, Japanese plum, and peach trees in Italy, Spain, France, Hungary, Turkey, Jordan, and Australia (1,2,4). During the summers of 2008 and 2010, leaf samples of old Czech local plum cultivars were obtained from the Holovousy collection and assessed for the presence of viruses belonging to the Capillovirus, Trichovirus, and Foveavirus genera using the polyvalent degenerate oligonucleotides (PDO) nested reverse transcription (RT)-PCR test (3). Following amplification from total RNAs extracts, the amplicons were cloned and several clones were sequenced for each plant sample. In plum (Prunus domestica) cv. Babce, a mixture of amplicons was observed and BlastN and BlastX analyses of the obtained sequences revealed the presence of ACLSV and APCLSV. The 310-bp APCLSV amplicon (GenBank Accession No. JN790294) showed highest identity (82.9% in nucleotide sequence and 97.1% in amino acid sequence) with the Sus2 isolate of APCLSV (4) and clustered with APCLSV isolates in a phylogenetic analysis. APCLSV infection was further confirmed with an APCLSV-specific RT-PCR assay (4), which yielded a product of the expected 205-bp size (GenBank Accession No. JN653070) with closest homology again to the Sus2 APCLSV isolate (83.4 and 94.3% nucleotide and amino acid identity, respectively). To our knowledge, this finding represents the first detection of APCLSV in domestic plums in the Czech Republic, extending our vision of APCLSV diversity and its geographic distribution. For unknown reasons, APCLSV has almost always been reported in mixed infection with ACLSV (1,2,4) and the situation in cv. Babce does not deviate from this trend. This has greatly hindered the analysis of the pathogenicity of APCLSV, a situation further complicated in the current case because the Babce cultivar was also infected by Plum pox virus. References: (1) M. Barone et al. Acta Hortic. 781:53, 2008. (2) T. Candresse et al. Virus and Virus-Like Diseases of Pome and Stone Fruit Trees. A. Hadidi et al., eds. The American Phytopathological Society, St. Paul, MN, 2011. (3) X. Foissac et al. Phytopathology 95:617, 2005. (4) D. Liberti et al. Phytopathology 95:420, 2005.

Genetic variability of Plum pox virus isolates in the Czech Republic

2008 ◽  
Vol 121 (4) ◽  
pp. 513-517 ◽  
Author(s):  
Sébastien Gadiou ◽  
Dana Šafářová ◽  
Milan Navrátil
Keyword(s):  
Czech Republic ◽  
Genetic Variability ◽  
Plum Pox Virus ◽  
The Czech Republic ◽  
Virus Isolates

scholarly journals Viruses of blackthorn and road-bordering trees of plum, myrobalan, sweet and sour cherries in the Czech Republic

2008 ◽  
Vol 43 (No. 1) ◽  
pp. 1-4 ◽  
Author(s):  
J. Polák
Keyword(s):  
Czech Republic ◽  
Mosaic Virus ◽  
Sour Cherry ◽  
Ringspot Virus ◽  
Dwarf Virus ◽  
Plum Pox Virus ◽  
The Czech Republic ◽  
Prune Dwarf Virus ◽  
Prunus Necrotic Ringspot Virus ◽  
Leafroll Virus

The distribution of <i>Plum pox virus</i> (PPV), <i>Prune dwarf virus</i> (PDV), <i>Prunus necrotic ringspot virus</i> (PNRSV), <i>Apple chlorotic ringspot virus</i> (ACLSV) and <i>Apple mosaic virus</i> (ApMV) in naturally growing shrubs of blackthorn and road-bordering trees of plum and myrobalan, and of PPV, PDV, PNRSV and <i>Cherry leafroll virus</i> (CLRV) in sweet and sour cherry trees were investigated. The most widely distributed viruses were PPV in plums (74% of the investigated trees were infected); PPV, PDV, and PNRSV in myrobalans (26%, 11% and 18%, respectively), PDV in blackthorns (27%), and PDV and PNRSV in cherries (25% and 22%). PPV was not detected in sweet and sour cherries. The incidence of ACLSV and ApMV was negligible in individually growing trees of the genus Prunus in the Czech Republic.

scholarly journals To the distribution of PPV-M strain in selected orchards of apricots and peaches in the Czech Republic

2011 ◽  
Vol 31 (No. 2) ◽  
pp. 44-46
Author(s):  
J. Polák
Keyword(s):  
Czech Republic ◽  
Plum Pox Virus ◽  
The Czech Republic

The presence and distribution of PPV-M strain of Plum pox virus were investigated in selected orchards of apricots and peaches in the Czech Republic. PPV-M was found to be distributed in apricot and peach orchards planted with nursery material imported from abroad. The presence of PPV-M was not proved in orchards planted with trees from local nurseries. The absence of PPV-M in a majority of spontaneous PPV hosts was proved previously. PPV-M strain appears to have been introduced recently. &nbsp;

scholarly journals Sporadic distribution of Plum pox virus M strain in natural sources in the Czech Republic

2011 ◽  
Vol 32 (No. 3) ◽  
pp. 85-88
Author(s):  
J. Polák ◽  
J. Pívalová
Keyword(s):  
Monoclonal Antibodies ◽  
Czech Republic ◽  
Central Europe ◽  
Polyclonal Antibodies ◽  
Plum Pox Virus ◽  
The Czech Republic ◽  
Natural Sources ◽  
Serological Typing ◽  
Natural Hosts

The presence and distribution of M strain of Plum pox virus (PPV-M) were investigated in natural hosts of&nbsp;Sharka, plums, myrobalans and blackthorns in the Czech Republic. Leaves or flowers of trees were evaluated for the presence of PPV by specific polyclonal antibodies at first. PPV infected samples were investigated for the presence of PPV-M by strain specific monoclonal antibodies. 102 PPV isolates from plum, 81 from myrobalan and 25 from blackthorn were typed. PPV-M was detected in six plum trees, six myrobalan trees and in one shrub of blackthorn. Sporadic incidence of PPV-M was proved in all investigated areas of the Czech Republic. Molecular and serological typing of different PPV strains in natural hosts, plum, apricot, and peach orchards was proposed to realize in Central Europe. &nbsp;

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