scholarly journals Identification and genetic diversity of Acer ibericum (Aceraceae) in South Caucasus

2021 ◽  
Vol 67 (No. 9) ◽  
pp. 420-426
Author(s):  
Juraj Paule ◽  
Nani Goginashvili ◽  
Irina Tvauri ◽  
Ladislav Paule ◽  
Ekaterine Gabashvili ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Marker ◽  
Internal Transcribed Spacer ◽  
Population Genetic ◽  
National Park ◽  
Its Sequence ◽  
South Caucasus ◽  
Conservation Measures

Internal transcribed spacer (ITS) sequence was tested in Acer ibericum for its suitability as a barcoding as well as population genetic marker. The marker was reliably used to identify A. ibericum as well as to gain preliminary insights into the genetic diversity of Georgian populations. MegaBLAST searches of studied samples against the GenBank database revealed that all studied accessions could be considered Acer ibericum. Populations from Vashlovani National Park revealed the presence of unique ribotypes, and together with the population from Shavi Mountain, they were identified as the most genetically diverse ones. Based on presented data the populations from Eastern Georgia should be prioritized if conservation measures are planned.

Genetic diversity of Peucedanum japonicum using Internal Transcribed Spacer (ITS) sequence

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
YH Kim ◽  
JA Ryuk ◽  
BS Ko ◽  
JW Lee ◽  
SE Oh ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Internal Transcribed Spacer ◽  
Its Sequence

Genotypic Characterization of Rhizopus Spp. Tempeh and Usar: Traditional Inoculum of Tempeh in Indonesia

2020 ◽  
Vol 14 (3) ◽  
pp. 3
Author(s):  
Tati Barus ◽  
Jason Wiranata Sanjaya ◽  
Anastasia Tatik Hartanti ◽  
Adi Yulandi ◽  
Vivitri Dewi Prasasty ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Internal Transcribed Spacer ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Its Sequence ◽  
Rhizopus Microsporus ◽  
Rhizopus Delemar

Abstract. Soybeans tempeh (tempeh) is processed by fermentation using Rhizopus spp. Tempeh is an important source of protein in Indonesia. The traditional inoculum in fermentation locally is known as Usar which is made from the leaves of Hibiscus tiliaceus. However, Rhizopus information from Usar is still limited. Therefore, this study aims to identify and investigate the genetic diversity of Rhizopus species from Usar and tempeh based on the Internal Transcribed Spacer (ITS) sequence and the Random Amplified Polymorphic DNA (RAPD) markers. Twenty-three Rhizopus strains were isolated from Usar and ten Rhizopus strains were isolated from tempeh. Based on ITS sequences, the isolates were similar to R Rhizopus microsporus (30 isolates) and Rhizopus delemar (3 isolates) with 98-99% similarity. The genetics of R. microsporus and R. delemar are varied and different from the genetics of R. microsporus from tempeh. The growth temperature of R. microsporus varies from 33 to 48°C and R. delemar can grow to a maximum at 33°C. The role of R. microsporus and R. delemar from Usar in determining the quality of tempeh is still limited. Therefore, it needs to be investigated further.

scholarly journals Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker

2019 ◽  
Vol 20 (3) ◽  
pp. 847-852
Author(s):  
TATI BARUS ◽  
RONALDO HALIM ◽  
ANASTASIA TATIK HARTANTI ◽  
PAULUS KEVIN SAPUTRA
Keyword(s):  
Genetic Diversity ◽  
Internal Transcribed Spacer ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Its Sequences ◽  
Its Sequence ◽  
Rhizopus Microsporus ◽  
Universal Primer ◽  
Cluster 2

Abstract. Barus T, Halim R, Hartanti AT, Saputra PK. 2019. Genetic diversity of Rhizopus microsporus from traditional inoculum of tempeh in Indonesia based on ITS sequences and RAPD marker. Biodiversitas 20: 847-852. The main microorganism for tempeh fermentation is Rhizopus microsporus. These days, many tempeh producers use commercial inoculum, such as ‘Raprima’ as resource of R. microsporus. As a result, the genetic diversity of R. microsporus that had been reported in Indonesia has diminished. Information about genetic diversity is needed as a basis to select R. microsporus as tempeh inoculum. This research aims to investigate the genetic diversity of R. microsporus from waru leaves based on Internal Transcribed Spacer (ITS) Sequence and Random Amplified Polymorphic DNA (RAPD) markers. A total of 25 R. microsporus were isolated from traditional inoculum waru leaves (Inoculum 1) and traditional inoculum other than waru leaves (Inoculum 2). Amplification of ITS sequence was done using universal primer pairs of ITS-4 and ITS-5. Amplification of RAPD markers was done using primers OPC-08, OPC-19, OPQ-6, R-108, OPA-09 and OPJ-20. ITS sequence was not sufficient to compare the similarities among R. microsporus. On the other hand, RAPD markers successfully compared the similarities among 25 R. microsporus. A total of 25 R. microsporus were divided into 9 clusters. R. microsporus from Inoculum 1 grouped into Cluster 1, Cluster 3 and Cluster 4-8. Inoculum 2 grouped into Cluster 2 and Cluster 9. R. microsporus from tempeh grouped into Cluster 4 and was different from Inoculum 1 and Inoculum 2, except for TB3.

scholarly journals A Meta-Analytical Investigation of the Gap between Measured and Predicted Inter-Population Genetic Diversity in Species of High Conservation Concern—The Case of the Critically Endangered European Mink Mustela lutreola L., 1761

Genes ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1555
Author(s):  
Jakub Skorupski ◽  
Johan Michaux ◽  
Przemysław Śmietana
Keyword(s):  
Genetic Diversity ◽  
Population Genetic ◽  
Meta Analysis ◽  
Critically Endangered ◽  
Northeastern Russia ◽  
Mustela Lutreola ◽  
Genetic Studies ◽  
European Mink ◽  
Population Genetic Diversity ◽  
Conservation Measures

Although properly designed sampling in population genetic studies is of key importance for planning evidence-informed conservation measures, sampling strategies are rarely discussed. This is the case for the European mink Mustela lutreola, a critically endangered species. In order to address this problem, a meta-analysis aiming to examine the completeness of mtDNA haplotype sampling in recent studies of M. lutreola inter-population genetic diversity was conducted. The analysis was performed using the sample-size-based rarefaction and extrapolation sampling curve method for three populations—the Northeastern (Russia, Belarus and Estonia), the Western (France and Spain), and the Southeastern (Romania). The extrapolated values of the Shannon–Wiener index were determined, assuming full sample coverage. The gap between the measured and predicted inter-population genetic diversity was estimated, indicating that the identified level of sample coverage was the lowest for the NE population (87%), followed by the SE population (96%) and the W population (99%). A guide for sampling design and accounting for sampling uncertainty in future population genetic studies on European mink is provided. The relatively low sample coverage for the Russian population clearly indicates an urgent need to take conservation measures for European mink in this country.

scholarly journals Meta-analysis of a polymorphic surface glycoprotein of the parasitic protozoa Cryptosporidium parvum and Cryptosporidium hominis

2009 ◽  
Vol 137 (12) ◽  
pp. 1800-1808 ◽  
Author(s):  
G. WIDMER
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Marker ◽  
Cryptosporidium Parvum ◽  
Meta Analysis ◽  
Surface Glycoprotein ◽  
Its Sequence ◽  
Marked Contrast ◽  
Cryptosporidium Hominis ◽  
Glycoprotein Gene

SUMMARYDue to its extensive polymorphism, a partial sequence of the Cryptosporidium surface glycoprotein gene gp60 has been frequently used as a genetic marker. I explored the global diversity of this protein, and compared its sequence diversity in Cryptosporidium parvum and Cryptosporidium hominis. In marked contrast to the geographical partition of C. parvum and C. hominis multi-locus genotypes, gp60 allelic groups showed no evidence of segregating in space, or of differing with respect to geographical diversity. Globally, genetic diversity of C. hominis gp60 exceeded that of C. parvum. Within C. parvum, gp60 alleles originating from human isolates were more diverse than those infecting ruminants. Phylogenetic analysis grouped gp60 sequences into a small number of relatively homogenous allelic groups, with only a small number of alleles having evolved independently. With the notable exception of a group of alleles restricted to humans, C. parvum alleles are found in ruminants and humans.

KERAGAMAN GENETIK INDUK UDANG WINDU DI PERAIRAN SELATAN PANGANDARAN DAN BINUANGEUN, JAWABARAT

2018 ◽  
Vol 5 (3) ◽  
Author(s):  
Ratu Siti Aliah
Keyword(s):  
Genetic Diversity ◽  
Population Genetic ◽  
Gene Locus ◽  
Penaeus Monodon ◽  
12S Rrna ◽  
Brood Stock ◽  
Population Genetic Diversity ◽  
Result Show ◽  
Mtdna Diversity

An evaluation of the Black Tiger Brood Stock (Penaeus monodon) genetic diversity of Pangandaran and Binuangeun was conducted by using the mtDNA diversity of two gene locus of CO I and 12S rRNA to understand their population genetic diversity. The result show that the brood stock of Pangandaran has 17 haplotipe, while from Binuangeun has 13 haplotipe. The result indicated that the genetic diversity of the Balck Tiger brood stock of Pangandaran was higher than thatBinuangeun.Key words : Genetic diversity, Black Tiger brood stock, Pangandaran, Binuangeun

Microsatellite analysis of population genetic diversity in Triplophysa xiangxiensis

2013 ◽  
Vol 37 (1) ◽  
pp. 26 ◽  
Author(s):  
Yanhong YAO ◽  
Lingfu KONG ◽  
Dengqiang WANG ◽  
Wenhui HE ◽  
Li HE ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Genetic ◽  
Microsatellite Analysis ◽  
Population Genetic Diversity

Allozyme variation and an estimate of the inbreeding coefficient in the coffee berry borer, Hypothenemus hampei (Coleoptera: Scolytidae)

1995 ◽  
Vol 85 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Philippe Borsa ◽  
D. Pierre Gingerich
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Population Genetic ◽  
New Caledonia ◽  
Allozyme Variation ◽  
Inbreeding Coefficient ◽  
Hypothenemus Hampei ◽  
Coffee Berry ◽  
Biological Features ◽  
High Degree

AbstractSeven presumed Mendelian enzyme loci (Est-2, Est-3, Gpi, Idh-l, Idh-2, Mdh-2 and Mpi) were characterized and tested for polymorphism in coffee berry borers, Hypothenemus hampei (Ferrari), sampled in Côte d′Ivoire, Mexico and New Caledonia. The average genetic diversity was H = 0.080. Two loci, Mdh-2 and Mpi were polymorphic, and thus usable as genetic markers. The population structure of H. hampei was analysed using Weir & Cockerham's estimators of Wright's F-statistics. A high degree of inbreeding (f = 0.298) characterized the elementary geographic sampling unit, the coffee field. The estimate of gene flow between fields within a country was Nm = 10.6 and that between countries was Nm = 2. The population genetic structure in H. hampei could be related to its known population biological features and history.

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