scholarly journals Haplotype analysis within quantitative trait locus affecting intramuscular fat content on porcine chromosome

2011 ◽  
Vol 56 (No. 12) ◽  
pp. 521-528
Author(s):  
S. Sato ◽  
C. Ohnishi ◽  
Y. Uemoto ◽  
E. Kobayashi
Keyword(s):  
Quantitative Trait ◽  
Haplotype Analysis ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Porcine Chromosome ◽  
Large White ◽  
Positional Candidate ◽  
Synonymous Substitutions ◽  
Trait Locus ◽  
Intramuscular Fat Content

Previous results of fine mapping for quantitative trait loci affecting intramuscular fat content identified a 3.0-Mb chromosome interval on porcine chromosome 7, which contains at least 9 genes, based on the pig genome assembly. Therefore, we proposed these nine genes (LOC100154481, LOC100155711, LOC100155276, SPATA7, PTPN21, ZCH14, EML5, TTC8, and FOXN3) as positional candidate genes. The coding exons of the nine genes were characterized, and 45 polymorphisms were detected in F<sub>2</sub> Duroc &times; Meishan population. Within the nine genes, 10 non-synonymous substitutions and 1 insertion were genotyped among three European breeds (Landrace, Large White, and Duroc) and 1 Chinese breed (Meishan). Genotyping data was used to perform the haplotype analysis. Polymorphisms were found in all the studied genes, except ZCH14. We surveyed the frequency of 33 haplotypes that formed non-synonymous substitutions in four breeds. One of them was distributed widely in the Landrace, Large White, and Meishan breeds, but not in Duroc. Each breed had different major haplotypes. &nbsp;

scholarly journals Variability in the leptin, leptin receptor and heart fatty acid binding protein genes in relationship with meat quality traits in pigs

2005 ◽  
Vol 53 (4) ◽  
pp. 87-94
Author(s):  
Renata Mikolášová ◽  
Tomáš Urban
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Leptin Receptor ◽  
Binding Protein ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Large White ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Intramuscular Fat Content

The leptin (LEP-HinfI), leptin receptor (LEPR-HpaII) and heart fatty acid binding protein (H-FABP-HinfI) genes and their genotypes combination (LEP-HinfI *LEPR-HpaII) were tested for associations with the pH1, pH24, myoglobin content (mg/100 g), intramuscular fat content (%) and remission (%). The genotypes were determined in Large White, Landrace and Duroc breeds (n = 106, 56 and 4, respectively). The allele frequencies were: LEP-HinfI: C = 0.133 T = 0.867; LEPR-HpaII: A = 0.331 B = 0.669; H-FABP-HinfI: H = 0.745 h = 0.255. The populations of breeds were in the genetic equilibrium according to the χ2 test in the tested loci. The combinations of LEP-HinfI and LEPR-HpaII were significantly associated with the pH24 and remission. The H-FABP-HinfI locus was significantly associated with intramuscular fat content.

scholarly journals Muscle transcriptome analysis identifies genes involved in ciliogenesis and the molecular cascade associated with intramuscular fat content in Large White heavy pigs

PLoS ONE ◽  
2020 ◽  
Vol 15 (5) ◽  
pp. e0233372 ◽  
Author(s):  
Martina Zappaterra ◽  
Silvia Gioiosa ◽  
Giovanni Chillemi ◽  
Paolo Zambonelli ◽  
Roberta Davoli
Keyword(s):  
Transcriptome Analysis ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Large White ◽  
Intramuscular Fat Content

The X Chromosome harbors quantitative trait loci for backfat thickness and intramuscular fat content in pigs

2000 ◽  
Vol 11 (9) ◽  
pp. 800-802 ◽  
Author(s):  
Barbara Harlizius ◽  
Annemieke P. Rattink ◽  
Dirk J. de Koning ◽  
Marilyne Faivre ◽  
Ruth G. Joosten ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
X Chromosome ◽  
Quantitative Trait ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Backfat Thickness ◽  
Trait Loci ◽  
Intramuscular Fat Content

scholarly journals Identification of QTL with effects on intramuscular fat content and fatty acid composition in a Duroc × Large White cross

BMC Genetics ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 55 ◽  
Author(s):  
Marie-Pierre Sanchez ◽  
Nathalie Iannuccelli ◽  
Benjamin Basso ◽  
Jean-Pierre Bidanel ◽  
Yvon Billon ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Large White ◽  
Intramuscular Fat Content

scholarly journals Comparative Microbial Profiles of Colonic Digesta between Ningxiang Pig and Large White Pig

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1862
Author(s):  
Linfeng Lei ◽  
Zhaobin Wang ◽  
Jianzhong Li ◽  
Huansheng Yang ◽  
Yulong Yin ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Fatty Acid Biosynthesis ◽  
16S Rrna Gene Sequencing ◽  
Short Chain Fatty Acids ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Rrna Gene ◽  
Scaling Analysis ◽  
Large White ◽  
Intramuscular Fat Content

Sixteen 35-day-old piglets, including eight Large White (LW) piglets (a lean-type pig breed) and eight Ningxiang (NX) piglets (a fatty-type Chinese Indigenous pig breed), were fed the same diet for 105 days. NX pigs had higher intramuscular fat content than LW pigs (p < 0.05). According to 16S rRNA gene sequencing, the relative abundances of the genera Ruminococcaceae_NK4A214_group, Parabacteroides, Christensenellaaceae_R-7_group and Ruminiclostridium were higher, whereas the abundances of Prevotellaceae_NK3B31_group, Prevotella, Subdoligranulum and Faecalibacterium were lower, in the colon of NX pigs compared to that of LW pigs. Nonmetric multidimensional scaling analysis revealed that the microbiota of the two pig breeds clustered separately along the principal coordinate axis. Furthermore, functional prediction of the bacterial communities suggested higher fatty acid biosynthesis in NX pigs. NX pigs also exhibited lower concentrations of total short-chain fatty acids, propionate and butyrate in the colon (p < 0.05). These findings suggest that NX pigs exhibited higher intramuscular fat content and backfat thickness than LW pigs. The bacterial communities in the colon of NX pigs were also more diverse than those in the colon of LW pigs, which might be used as a potential metabolomics mechanism to research different breeds of pigs.

scholarly journals Efect of intramuscular fat content on physical-chemical parameters of pork from Mangalitsa and their crossbreed

10.5219/1095 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 422-428
Author(s):  
Petra Lípová ◽  
Ondrej Debrecéni ◽  
Ondřej Bučko ◽  
Klára Vavrišínová
Keyword(s):  
Moisture Content ◽  
Protein Content ◽  
Live Weight ◽  
Cholesterol Content ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Post Mortem ◽  
Chemical Parameters ◽  
Large White ◽  
Intramuscular Fat Content

The aim of study was to evaluate the effect of intramuscular fat content on physical parameters and proximate composition in musculus longissimus dorsi (MLD) from Mangalitsa breed and Slovak Large White x Mangalitsa crossbreed. In the study, sixteen pigs of Mangalitsa and twenty-two pigs of Slovak Large White x Mangalitsa crossbreed were used. The pigs were reared under intensive condition and all animals were fed ad libitum with complete fattening feed mixture. The fattening period started from 30 kg of live weight. Then the pigs were slaughtered at 100 kg of live weight. Chemical analysis showed that MLD from Mangalitsa had lower protein content, higher moisture content (p <0.05) and higher content of intramuscular fat compared to Slovak Large White x Mangalitsa crossbreed. As regarding the cholesterol content in MLD, no significant differences were found between genotypes, but the cholesterol content was higher in MLD from Mangalitsa than in MLD from crossbreeds. The MLD from Mangalitsa exhibited lower CIE L* (p <0.01) and CIE b* (p <0.01) values 45 min post mortem compared to crossbreeds. Colour parameters increased after 7 days post mortem, which is normal due to the maturing process of the meat. Then the CIE L* value was lower in MLD from Mangalitsa (p <0.01), but CIE a* value was higher in relation to crossbreeds (p <0.05). As regards the Warner-Bratzler shear force, the meat from Mangalitsa was tenderer than in crossbreeds (p <0.05). Intramuscular fat in the meat positively correlated with colour parameter CIE a* (r = 0.324; p <0.05) as well as cholesterol content (r = 0,656; p <0.001). In contrast in the study was found negative correlations between intramuscular fat in meat with moisture content (r = -0.399; p <0.05) and protein content (r = -0.812; p <0.001). It can be concluded that the percentage of intramuscular fat significantly influenced the physical and the chemical parameters of pork. The meat from Mangalitsa is more suitable for production of special meat products (fermented and smoked).

scholarly journals Evidence Against the Causal Relationship Between a Putative Cis-Regulatory Variant of MYH3 and Intramuscular Fat Content in Pigs

2021 ◽  
Vol 8 ◽  
Author(s):  
Cong Huang ◽  
Liepeng Zhong ◽  
Xiaoxiao Zou ◽  
Yizhong Huang ◽  
Liping Cai ◽  
...  
Keyword(s):  
Causal Relationship ◽  
Meat Quality ◽  
Large Scale ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Commercial Application ◽  
Large White ◽  
Yorkshire Pigs ◽  
Causal Mutation ◽  
Intramuscular Fat Content

Improving meat quality has become the main goal of modern pig breeding. Intramuscular fat content (IMF) is an important trait influencing meat quality of livestock, but the molecular mechanism behind this trait is still unclear. Recently, Cho et al. reported the discovery of the first causal mutation affecting IMF and red flesh color (a*) in pigs, namely XM_013981330.2:g.−1805_−1810del, a 6-bp deletion variant in the porcine MYH3 promoter region. The objective of this study was to reassess the causality of this mutation for its potential commercial application. By Sanger sequencing, we firstly identified several new variants (including a 4-bp deletion) at or near the 6-bp deletion site, which formed four haplotypes in multiple breeds. Unexpectedly, the 6-bp deletion allele, previously determined as the MYH3 Q allele because of its significantly positive effect on IMF and a*, was found not only in Chinese indigenous breeds, but also in four western commercial breeds with relatively lower IMF levels, including Duroc, Large White, Landrace and Pietrain. More surprisingly, we found that the MYH3 Q allele and the haplotypes harboring it had no significant effects on IMF, marbling and color score in three large-scale divergent pig populations: the heterogeneous F6 and F7 pigs and commercial crossbred Duroc × (Landrace × Yorkshire) pigs. Transient transfection analysis in porcine satellite cells showed that the 6-bp deletion variants had a negligible effect on transcription of reporter gene, but could attenuate the MRF (myogenesis regulatory factors)-induced increase in luciferase activity of the MYH3 promoter vector. The MYH3 protein level in muscle did not differ significantly among the haplotype groups. Therefore, our results cannot support the causal relationship between the 6-bp deletion in MYH3 and IMF trait, suggesting that the causal mutation for the IMF QTL on SSC12 needs to be further identified.

scholarly journals Quantitative Trait Gene Responsible for Intramuscular Fat Content in the Rat

2011 ◽  
Vol 10 (7) ◽  
pp. 841-846
Author(s):  
Hideki Tanomura ◽  
Takuji Yamamoto ◽  
Youji Muramatsu ◽  
Takeshi Ohta ◽  
Takahisa Yamada
Keyword(s):  
Quantitative Trait ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Intramuscular Fat Content ◽  
Quantitative Trait Gene

scholarly journals Detection of Quantitative Trait Loci for Backfat Thickness and Intramuscular Fat Content in Pigs (Sus scrofa)

Genetics ◽  
1999 ◽  
Vol 152 (4) ◽  
pp. 1679-1690 ◽  
Author(s):  
Dirk J de Koning ◽  
Luc L G Janss ◽  
Annemieke P Rattink ◽  
Pieter A M van Oers ◽  
Beja J de Vries ◽  
...  
Keyword(s):  
Intramuscular Fat ◽  
Interval Mapping ◽  
Fat Content ◽  
Backfat Thickness ◽  
Chromosome 2 ◽  
Large White ◽  
Cross Model ◽  
Suggestive Evidence ◽  
Line Cross ◽  
Intramuscular Fat Content

Abstract In an experimental cross between Meishan and Dutch Large White and Landrace lines, 619 F2 animals and their parents were typed for molecular markers covering the entire porcine genome. Associations were studied between these markers and two fatness traits: intramuscular fat content and backfat thickness. Association analyses were performed using interval mapping by regression under two genetic models: (1) an outbred line-cross model where the founder lines were assumed to be fixed for different QTL alleles; and (2) a half-sib model where a unique allele substitution effect was fitted within each of the 19 half-sib families. Both approaches revealed for backfat thickness a highly significant QTL on chromosome 7 and suggestive evidence for a QTL at chromosome 2. Furthermore, suggestive QTL affecting backfat thickness were detected on chromosomes 1 and 6 under the line-cross model. For intramuscular fat content the line-cross approach showed suggestive evidence for QTL on chromosomes 2, 4, and 6, whereas the half-sib analysis showed suggestive linkage for chromosomes 4 and 7. The nature of the QTL effects and assumptions underlying both models could explain discrepancies between the findings under the two models. It is concluded that both approaches can complement each other in the analysis of data from outbred line crosses.

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