scholarly journals Effects of potassium fertilisation on late potato blight and yield – short communication

2018 ◽  
Vol 54 (No. 2) ◽  
pp. 87-91 ◽  
Author(s):  
Kowalska Jolanta ◽  
Drożdżyński Darius
Keyword(s):  
Synergistic Effect ◽  
Alternative Treatment ◽  
Short Communication ◽  
Maximum Dose ◽  
Infestation Rate ◽  
Late Potato ◽  
Potato Blight ◽  
Soil Application ◽  
Potato Yields ◽  
Uniform Moisture

Potato yields and infestation by P. infestans are related to the supply of potassium. Potassium was applied as soil fertilisation combined with split foliar applications or only as split foliar treatments at a maximum dose of 150 kg/ha K<sub>2</sub>O in both strategies, Lord and Ditta cultivars were used. Additionally, water spraying was included as an alternative treatment in order to maintain uniform moisture in the rows of plants. Plants fertilised with foliar spraying only were more infested than plants fertilised with combined methods. The fertiliser increased the protection impact of copper treatments against P. infestans. This may suggest a possible synergistic effect in reducing the symptoms of the disease, however not always statistically significant in both cultivars. Plants sprayed with water but without soil application of fertiliser showed a statistically significantly higher infestation rate, both in Lord and Ditta cultivars, compared to plants with soil application of fertiliser but without watering.

scholarly journals Mitochondrial targeting of glycolysis in a major lineage of eukaryotes

2018 ◽  
Author(s):  
Carolina Río Bártulos ◽  
Matthew B. Rogers ◽  
Tom A. Williams ◽  
Eleni Gentekaki ◽  
Henner Brinkmann ◽  
...  
Keyword(s):  
Enzyme Assays ◽  
Mitochondrial Targeting ◽  
Late Potato ◽  
Potato Blight ◽  
Large Group ◽  
Unicellular Algae ◽  
Targeting Signals ◽  
Triton X ◽  
Eukaryotic Genomes ◽  
New Strategies

Glycolysis is a major cytosolic catabolic pathway that provides ATP for many organisms1. Mitochondria play an even more important role in the provision of additional cellular ATP for eukaryotes2. Here, we show that in many stramenopiles, the C3 part of glycolysis is localised in mitochondria. We discovered genuine mitochondrial targeting signals on the six last enzymes of glycolysis. These targeting signals are recognised and sufficient to import GFP into mitochondria of a heterologous host. Analysis of eukaryotic genomes identified these targeting signals on many glycolytic C3 enzymes in a large group of eukaryotes found in the SAR supergroup3, in particular the stramenopiles. Stramenopiles, or heterokonts, are a large group of ecologically important eukaryotes that includes multi- and unicellular algae such as kelp and diatoms, but also economically important oomycete pathogens such as Phytophthora infestans. Confocal immunomicroscopy confirmed the mitochondrial location of glycolytic enzymes for the human parasite Blastocystis. Enzyme assays on cellular fractions confirmed the presence of the C3 part of glycolysis in Blastocystis mitochondria. These activities are sensitive to treatment with proteases and Triton X-100 but not proteases alone. Our work clearly shows that core cellular metabolism is more plastic than previously imagined and suggests new strategies to combat stramenopile pathogens such as the causative agent of late potato blight, P. infestans.

scholarly journals Thirty years of the PROINPA Foundation's contribution to Bolivian agriculture: Case of “Marcela” potato crop

2020 ◽  
Vol 23 (2) ◽  
pp. 66-73
Author(s):  
J. Gabriel
Keyword(s):  
Climate Change ◽  
Phytophthora Infestans ◽  
Drought Resistance ◽  
Potato Crop ◽  
Potato Production ◽  
Alternative Strategy ◽  
Late Potato ◽  
Potato Blight

With the objetive of recognizing Bolivian researchers, who are not always mentioned, we have written this simple but significant document, in which the case of potato cultivation “Marcela”, which is being widely used in the valleys from Tarija and Potosí heights mainly, due to its tolerance to drought, resistance to late potato blight (Phytophthora infestans) and precocity, with yields ranging from 15 to 20 t ha-1. This cultivar was assumed by INIAF as an alternative strategy to deal with reducing the effects of climate change on potato production.

scholarly journals Honey as a Strategy to Fight Candida tropicalis in Mixed-Biofilms with Pseudomonas aeruginosa

Antibiotics ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 43 ◽  
Author(s):  
Liliana Fernandes ◽  
Ana Oliveira ◽  
Mariana Henriques ◽  
Maria Elisa Rodrigues
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fluorescence Microscopy ◽  
Synergistic Effect ◽  
Antimicrobial Agent ◽  
Candida Tropicalis ◽  
Alternative Treatment ◽  
Antimicrobial Agents ◽  
Colony Forming Units ◽  
Antimicrobial Combinations

Fungal contaminations with Candida species are commonly responsible for several infections, especially when associated to bacteria. The therapeutic approach commonly used is being compromised due to microbial resistances of these microorganisms to antimicrobial agents, especially in biofilm. The use of honey as an antimicrobial agent has been emerging as a valuable solution and proving its potential in planktonic and in biofilm cells. This work aims to assess the effect of different honeys on biofilms of Candida tropicalis and Pseudomonas aeruginosa. The effect of Portuguese heather (PH) and manuka honeys on planktonic growth of Candida was initially evaluated by determination of the minimum inhibitory concentrations (MIC). Then, the same effect was evaluated in mixed biofilms, by colony-forming units numeration and fluorescence microscopy. The combinations of honey plus fluconazole and gentamicin were also tested. The results showed that the honeys tested enabled a great reduction of C. tropicalis, both in planktonic (12.5% and 25% of MIC for PH and manuka) and in biofilm. In polymicrobial biofilms, the use of PH and manuka honeys was revealed to be a promising choice and an alternative treatment, since they were able to reduce cells from both species. No synergistic effect was observed in antimicrobial combinations assays against polymicrobial biofilms.

Ultrastructure of primary spermatocyte in fish (Tilapia: Oreochromis niloticus): The synaptonemal complex

1986 ◽  
Vol 44 ◽  
pp. 288-289
Author(s):  
T. Guha ◽  
A. Q. Siddiqui ◽  
P. F. Prentis
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Synaptonemal Complex ◽  
Oreochromis Niloticus ◽  
Short Communication ◽  
Primary Spermatocyte ◽  
Mitotic Division ◽  
Meiotic Cell ◽  
Electron Microscope Level ◽  
Homologous Chromosomes

The Primary Spermatocytes represent a stage in spermatogenesis when the first meiotic cell division occurs. They are derived from Spermatogonium or Stem cell through mitotic division. At the zygotene phase of meiotic prophase the Synaptonemal complex appears in these cells in the space between the paired homologous chromosomes. Spermatogenesis and sperm structure in fish have been studied at the electron microscope level in a few species? However, no work has yet been reported on ultrastructure of tilapia, O. niloticus, spermatozoa and spermatogenetic process. In this short communication we are reporting the Ultrastructure of Primary Spermatocytes in tilapia, O. niloticus, and the fine structure of synaptonemal complexes seen in the spermatocyte nuclei.

Projected Structure of the Surface Protein of Sulfolobus Spec. B12 Determined to a Resolution of 1.0 NM by Cryo-Electronmicroscopy

1990 ◽  
Vol 48 (1) ◽  
pp. 102-103
Author(s):  
G. Lembcke ◽  
F. Zemlin
Keyword(s):  
Low Dose ◽  
Maximum Dose ◽  
Three Dimensional ◽  
Surface Protein ◽  
Protein S ◽  
Radiation Sensitivity ◽  
Specimen Preparation ◽  
Molecular Architecture ◽  
High Radiation ◽  
Fritz Haber

The thermoacidophilic archaebacterium Sulfolobus spec. B12 , which is closely related to Sulfolobus solfataricus , possesses a regularly arrayed surface protein (S-layer), which is linked to the plasma membrane via spacer elements spanning a distinct interspace of approximately 18 nm. The S-layer has p3-Symmetry and a lattice constant of 21 nm; three-dimensional reconstructions of negatively stained fragments yield a layer thickness of approximately 6-7 nm.For analysing the molecular architecture of Sulfolobus surface protein in greater detail we use aurothioglucose(ATG)-embedding for specimen preparation. Like glucose, ATG, is supposed to mimic the effect of water, but has the advantage of being less volatile. ATG has advantages over glucose when working with specimens composed exclusively of protein because of its higher density of 2.92 g cm-3. Because of its high radiation sensitivity electromicrographs has to be recorded under strict low-dose conditions. We have recorded electromicrographs with a liquid helium-cooled superconducting electron microscope (the socalled SULEIKA at the Fritz-Haber-lnstitut) with a specimen temperature of 4.5 K and with a maximum dose of 2000 e nm-2 avoiding any pre-irradiation of the specimen.

SHORT COMMUNICATION

1997 ◽  
Vol 61 (3) ◽  
pp. 275-279 ◽  
Author(s):  
S. MONNO ◽  
Y. MIZUSHIMA ◽  
N. TOYODA ◽  
T. KASHII ◽  
M. KOBAYASHI
Keyword(s):  
Short Communication

Short Communication: Synthesis of Polymer-Embedded Metal Clusters by Thermolysis of Mercaptides—Polymer Blends

Polymer News ◽  
2005 ◽  
Vol 30 (9) ◽  
pp. 296-300
Author(s):  
F. Esposito ◽  
V. Casuscelli ◽  
M. V. Volpe ◽  
G. Carotenuto ◽  
L. Nicolais
Keyword(s):  
Polymer Blends ◽  
Short Communication ◽  
Metal Clusters ◽  
Communication Synthesis

Short Communication: The "Half-Monomer" Concept for Sequence Analysis of Condensation Copolymers by NMR

Polymer News ◽  
2004 ◽  
Vol 29 (7) ◽  
pp. 228-229
Author(s):  
Maurizio Montaudo
Keyword(s):  
Sequence Analysis ◽  
Short Communication

Short communication Temperature dependence of F X reaction based holographic recording in electrolytically coloured KBr crystals

1996 ◽  
Vol 43 (8) ◽  
pp. 1759-1762
Author(s):  
ANDRIS OZOLS OLLI SALMINEN and ERIK RAITA
Keyword(s):  
Temperature Dependence ◽  
Short Communication ◽  
Holographic Recording
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