scholarly journals Evaluation of the genetic diversity of selected Fagus sylvatica L. populations in the Czech Republic using nuclear microsatellites

2017 ◽  
Vol 63 (No. 2) ◽  
pp. 53-61 ◽  
Author(s):  
Cvrčková Helena ◽  
Máchová Pavlína ◽  
Poláková Lucie ◽  
Trčková Olga
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Microsatellite Markers ◽  
Fagus Sylvatica ◽  
Tree Species ◽  
European Beech ◽  
Fixation Index ◽  
Detailed Knowledge ◽  
The Czech Republic ◽  
Adult Trees

Fagus sylvatica Linnaeus (European beech), the ecologically and economically most important broadleaved tree species in the Czech Republic, was strongly reduced in the past. Today there are efforts to increase the proportion of beech to ensure optimal forest tree species composition. When extensively reintroducing beech, it is important to acquire more detailed knowledge of genetic diversity. Thirteen important beech populations in different stands in the territory of the Czech Republic were genotyped using 12 polymorphic nuclear microsatellite markers. The genotypic data from adult trees imply genetic differences between the populations. The estimated genetic diversity expressed as Shannon’s information index ranged from 1.73 to 1.92. Thirteen beech populations showed excess homozygotes, as indicated by positive fixation index (F) values (F = 0.005–0.115). The pairwise F<sub>ST</sub> values indicated low genetic differentiation between the 13 Czech beech populations, because they were greater than zero, that means they confirmed the presence of population structuring in Czech European beech. Not significant linear correlations were observed between genetic and geographic distances of the 13 beech populations studied on the basis of microsatellite markers. Twelve microsatellite markers were verified as highly polymorphic and suitable for genotyping of European beech populations.

scholarly journals Microsatellite analysis of genetic diversity in Czech populations of European beech (Fagus sylvatica L.)

2020 ◽  
Vol 73 (1) ◽  
pp. 64-76
Author(s):  
Dagmar Zádrapová ◽  
Jiří Korecký ◽  
Jakub Dvořák ◽  
Zuzana Faltinová ◽  
Jan Bílý
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Fagus Sylvatica ◽  
Tree Species ◽  
European Beech ◽  
Forest Tree ◽  
Genetic Distances ◽  
Tree Species Composition ◽  
The Czech Republic ◽  
Primary Driver

Abstract European beech (Fagus sylvatica L.) is one of the most important broadleaved tree species in Europe both ecologically and economically. Nowadays, in the Czech Republic, beech is underrepresented in forest tree species composition, and there are tendencies to increase its proportion. When reintroducing beech, genetic variability, along with other factors, play a key role. The main aim of this study was to evaluate the genetic diversity of ten selected indigenous beech populations across the Czech Republic. Two hundred and fifty individuals were genotyped on 21 polymorphic nuclear microsatellite markers, which were amplified using two newly assembled multiplexes. According to the results, observed heterozygosity (Ho ) among populations ranged from 0.595 to 0.654 and expected heterozygosity (He ) from 0.650 to 0.678. That is comparable with the findings in other European studies. The high discriminatory power of the assembled multiplexes was confirmed by calculating the Probability of Identity among both unrelated and related individuals. Principal Coordinate Analysis (PCoA) based on Nei's genetic distances revealed that there are genetic differences among populations resulting in three approximate clusters (geographically north, south-east, and south-west). Nevertheless, the results implicate that on a geographical scale of the Czech Republic, the distance is unlikely to be the primary driver of genetic differentiation.

scholarly journals Models for predicting aboveground biomass of European beech (Fagus sylvatica L.) in the Czech Republic

2016 ◽  
Vol 61 (No. 2) ◽  
pp. 45-54 ◽  
Author(s):  
Vejpustková Monika ◽  
Zahradník Daniel ◽  
Čihák Tomáš ◽  
Šrámek Vít
Keyword(s):  
Czech Republic ◽  
Fagus Sylvatica ◽  
Aboveground Biomass ◽  
European Beech ◽  
The Czech Republic ◽  
Fagus Sylvatica L

scholarly journals Genetic diversity of Anadenanthera colubrina Vell. (Brenan) var cebil, a tree species from the South American subtropical forest as revealed by cpSSR markers

2011 ◽  
Vol 60 (1-6) ◽  
pp. 123-132 ◽  
Author(s):  
M. E. Barrandeguy ◽  
M.V. García ◽  
C. F. Argüelles ◽  
G. D. L. Cervigni
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Tree Species ◽  
Evolutionary Dynamics ◽  
Pairwise Comparison ◽  
Fixation Index ◽  
Haplotypic Diversity ◽  
Adult Trees ◽  
Minimum Spanning Network ◽  
Subdivided Populations

Abstract Anadenanthera colubrina var cebil is a tree species native to the Upper Parana Atlantic Forest where human activities have severely impacted causing deep fragmentation. Microsatellites are not available in this species. Therefore, the first objective of this study was to generate chloroplast simple sequence repeats (cpSSR) by cross-species transfer. Understanding the evolutionary dynamics of subdivided populations is an important matter. In this way, a first approach to the characterization of the haplotypic diversity within and between populations as well as the genetic structure of native Argentinean populations were the main goals of this study. Twenty four individuals from two populations of the Misiones province were studied and four cpSSR loci were tested. Two of them exhibited polymorphic patterns leading to the identification of 11 cpDNA haplotypes with high mean genetic diversity (GD=0.73). The minimum spanning network defined three clear groups which can be assigned to at least three subpopulations. AMOVA indicated that the total variance showed the highest percentage of variation (48%) within subpopulations with a fixation index (FST) statistically significant (FST=0.520; p<0.05). Brown’s two loci component analysis indicated that substructure population is present. Jost’s differentiation global index (Dest) was 0.049 while Dest pairwise comparison reflected a certain level of genetic structure. The high diversity level detected in the adult trees of A. colubrina var cebil from the populations under study could be due to recent human influence. In this way, the reduction in population size caused a reduction in the number of trees leading to surviving trees showing the historical diversity of the populations analyzed.

scholarly journals High Levels of Genetic Diversity in Salix viminalis of the Czech Republic as Revealed by Microsatellite Markers

2012 ◽  
Vol 5 (4) ◽  
pp. 969-977 ◽  
Author(s):  
Sviatlana O Trybush ◽  
Šárka Jahodová ◽  
Luďka Čížková ◽  
Angela Karp ◽  
Steven J Hanley
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Microsatellite Markers ◽  
Salix Viminalis ◽  
The Czech Republic

scholarly journals Genetic diversity of sessile oak populations in the Czech Republic

2022 ◽  
Vol 68 (No. 1) ◽  
pp. 8-18
Author(s):  
Jakub Dvořák ◽  
Jiří Korecký ◽  
Zuzana Faltinová ◽  
Dagmar Zádrapová
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Tree Species ◽  
Deciduous Tree ◽  
Boundary Line ◽  
The Czech Republic ◽  
Sessile Oak ◽  
Seed Sources ◽  
Forest Reproductive Material ◽  
Reproductive Material

The sessile oak is a broadleaved tree species of great ecological and silvicultural importance. Oaks are the second most widespread deciduous tree species in the Czech Republic, and ongoing climate change negatively affects the abundant and often monocultural Norway spruce. Therefore, a proportional increase of more resilient tree species such as sessile oak has emerged. This study aimed to depict population genetic diversity when analysing 272 individuals from 10 subpopulations selected across the Czech Republic. Targeted populations were chosen based on the minimal expected human impact on the stand (presumably autochthonous stands). All individuals were genotyped using 18 polymorphic microsatellite markers (SSRs) assembled into two amplification multiplexes. The high discriminatory power of SSR markers was tested and confirmed by the probability of identity analysis. The genetic differentiation of the subpopulations was low yet significant, quantified by Wright’s F-statistics within the range from 0.012 to 0.029. Based on discriminant analysis of principal components (DAPC), we detected two populations with geographic genetic correlation (the 15<sup>th</sup> meridian east being a north-south boundary line) and one with a distinct genetic pattern. We assume that the population might previously be established from seed sources outside the Czech Republic. Moreover, to some extent, our findings advocate the legitimacy of the legislative rules for forest reproductive material (FRM) transfer.

scholarly journals Genetic assessment of three Fagaceae species in forest restoration trials

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6958 ◽  
Author(s):  
Patcharawadee Thongkumkoon ◽  
Siriwadee Chomdej ◽  
Jatupol Kampuansai ◽  
Waranee Pradit ◽  
Pimubon Waikham ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Tree Species ◽  
Forest Restoration ◽  
Second Generation ◽  
Fixation Index ◽  
Seed Sample ◽  
Sample Collection ◽  
Entire Genome ◽  
Collection Method ◽  
Adult Trees

Restoring isolated patches of forest ecosystems in degraded landscapes could potentially lead to genetic loss and inbreeding. Therefore, this study determined the occurrence of genetic diversity among the tree species Castanopsis tribuloides, C. calathiformis, and Lithocarpus polystachyus all of which were proven previously to be effective native tree species in the restoration of upland evergreen forests in northern Thailand when using the seed sample collection method. We tested our hypothesis as to whether the genetic diversity of a plant population that had been planted from the seeds of 4–6 adult trees would be lower and whether incidences of fixation index (Fis) would be higher among the second generation seedlings of these three Fagaceae species in isolated forest restoration trial plots. Microsatellite primers were selected from the entire genome sequence of C. tribuloides and the genetic sequences of C. tribuloides, L. polystachyus, and C. calathiformis were analyzed. Our results indicated a high degree of genetic diversity (He) in C. tribuloides (0.736) and C. calathiformis (0.481); however, a low level of genetic diversity was observed in L. polystachyus (0.281) within the restored forest. The fixation index for the second generation of L. polystachyus and C. calathiformis in the restored forest showed evidence of inbreeding. These results imply the efficiency of the seed sample collection method and verify that it does not reduce the level of genetic diversity in C. tribuloides and C. calathiformis. However, it may result in incidences of an inbreeding phenomena, suggesting the need to increase the number of adult trees used at the seed collection stage.

Genetic diversity and differentiation in European beech (Fagus sylvatica L.) stands varying in management history

2007 ◽  
Vol 247 (1-3) ◽  
pp. 98-106 ◽  
Author(s):  
J. Buiteveld ◽  
G.G. Vendramin ◽  
S. Leonardi ◽  
K. Kamer ◽  
T. Geburek
Keyword(s):  
Genetic Diversity ◽  
Fagus Sylvatica ◽  
European Beech ◽  
Management History ◽  
Fagus Sylvatica L

scholarly journals Polymorphism of prolamin proteins of the grain of triticale varieties certifiedin the Czech Republic

2011 ◽  
Vol 51 (No. 4) ◽  
pp. 151-155 ◽  
Author(s):  
T. Vyhnánek ◽  
J. Bednář
Keyword(s):  
Genetic Diversity ◽  
Czech Republic ◽  
Cold Resistance ◽  
The Czech Republic ◽  
Baking Quality ◽  
The Individual

&nbsp;Genetic diversity was detected in 11 varieties of triticale registered in the Czech Republic by means of polymorphism of prolamin proteins using the PAGE ISTA method. The polymorphism of prolamin proteins allowed the differentiation of the individual triticale varieties in 2002 and 2003 harvests. On the basis of Dice&rsquo;s calculations of coefficients of similarity we discovered, in parallel with the uniform genotypes, genotypes with sister prolamin spectrums with a&nbsp;different percentage of participation in the respective years. A uniform spectrum was detected in the following varieties: Disco, Kolor, Lamberto, Marko, Presto, Sekundo, Ticino and Tricolor; Kitaro and Modus were dimorphous varieties. In 2003 three sister prolamin lines appeared in the variety Gabo and in 2004 only two. In 2003 a 5% admixture of a foreign genotype was detected in the variety Marko. Typical of the unknown genotype was the gliadin block Gld&nbsp;1B3, which is the marker of rye translocation T1BL.1RS, gene Sr31 with resistance to black rust, higher cold resistance and lower baking quality of the wheat. The prolamin proteins of triticale grain are suitable for the detection of the genetic diversity and for the assessment of varietal authenticity and purity in seed samples of triticale varieties registered in the Czech Republic.

scholarly journals Initial Survival and Development of Planted European Beech (Fagus sylvatica L.) and Small-Leaved Lime (Tilia cordata Mill.) Seedlings Competing with Black Cherry (Prunus serotina Ehrh.)

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 677
Author(s):  
Sarah L. Hasstedt ◽  
Peter Annighöfer
Keyword(s):  
Fagus Sylvatica ◽  
Tree Species ◽  
Survival Rates ◽  
Light Availability ◽  
European Beech ◽  
Prunus Serotina ◽  
Tilia Cordata ◽  
Black Cherry ◽  
Light Gradient ◽  
Fagus Sylvatica L

Black cherry (Prunus serotina Ehrh.) is considered one of the most invasive tree species in central Europe and causes problems for both nature conservation and silviculture. Besides mechanical control treatments, a suggested control method to prevent its ongoing spread is to underplant shade-tolerant native tree species. Therefore, we combined two mechanical treatments, with underplanting of European beech (Fagus sylvatica L.) or small-leaved lime (Tilia cordata Mill.) on fenced and unfenced plots. After the first growing season, survival rates were evaluated, and selected seedlings were destructively harvested to analyze their growth performance and leaf morphology in association with the different light regimes resulting from mechanical treatments Survival rates for both seedlings were very high (>95%). Survival rates were higher on fenced plots than on unfenced plots, most likely as result of browsing. The mortality of F. sylvatica decreased with increasing light availability on fenced plots. The mortality of T. cordata did not change along the light gradient. After one vegetation period no differences with respect to biomass allocation could be detected along the light gradient. However, the specific leaf areas of both species responded similarly, decreasing with increasing light availability. In summary, both species were able to establish and survive in the dense P. serotina understory and might have the potential to outcompete the invasive alien species in the long run.

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