Microsatellite analysis of genetic diversity in Czech populations of European beech (Fagus sylvatica L.)

Abstract European beech (Fagus sylvatica L.) is one of the most important broadleaved tree species in Europe both ecologically and economically. Nowadays, in the Czech Republic, beech is underrepresented in forest tree species composition, and there are tendencies to increase its proportion. When reintroducing beech, genetic variability, along with other factors, play a key role. The main aim of this study was to evaluate the genetic diversity of ten selected indigenous beech populations across the Czech Republic. Two hundred and fifty individuals were genotyped on 21 polymorphic nuclear microsatellite markers, which were amplified using two newly assembled multiplexes. According to the results, observed heterozygosity (Ho ) among populations ranged from 0.595 to 0.654 and expected heterozygosity (He ) from 0.650 to 0.678. That is comparable with the findings in other European studies. The high discriminatory power of the assembled multiplexes was confirmed by calculating the Probability of Identity among both unrelated and related individuals. Principal Coordinate Analysis (PCoA) based on Nei's genetic distances revealed that there are genetic differences among populations resulting in three approximate clusters (geographically north, south-east, and south-west). Nevertheless, the results implicate that on a geographical scale of the Czech Republic, the distance is unlikely to be the primary driver of genetic differentiation.

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Evaluation of the genetic diversity of selected Fagus sylvatica L. populations in the Czech Republic using nuclear microsatellites

Journal of Forest Science ◽

10.17221/88/2016-jfs ◽

2017 ◽

Vol 63 (No. 2) ◽

pp. 53-61 ◽

Cited By ~ 1

Author(s):

Cvrčková Helena ◽

Máchová Pavlína ◽

Poláková Lucie ◽

Trčková Olga

Keyword(s):

Genetic Diversity ◽

Czech Republic ◽

Microsatellite Markers ◽

Fagus Sylvatica ◽

Tree Species ◽

European Beech ◽

Fixation Index ◽

Detailed Knowledge ◽

The Czech Republic ◽

Adult Trees

Fagus sylvatica Linnaeus (European beech), the ecologically and economically most important broadleaved tree species in the Czech Republic, was strongly reduced in the past. Today there are efforts to increase the proportion of beech to ensure optimal forest tree species composition. When extensively reintroducing beech, it is important to acquire more detailed knowledge of genetic diversity. Thirteen important beech populations in different stands in the territory of the Czech Republic were genotyped using 12 polymorphic nuclear microsatellite markers. The genotypic data from adult trees imply genetic differences between the populations. The estimated genetic diversity expressed as Shannon’s information index ranged from 1.73 to 1.92. Thirteen beech populations showed excess homozygotes, as indicated by positive fixation index (F) values (F = 0.005–0.115). The pairwise FST values indicated low genetic differentiation between the 13 Czech beech populations, because they were greater than zero, that means they confirmed the presence of population structuring in Czech European beech. Not significant linear correlations were observed between genetic and geographic distances of the 13 beech populations studied on the basis of microsatellite markers. Twelve microsatellite markers were verified as highly polymorphic and suitable for genotyping of European beech populations.

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Testing of germination of spruce, pine and larch seed after 10 years from collection

Journal of Forest Science ◽

10.17221/74/2013-jfs ◽

2014 ◽

Vol 60 (No. 12) ◽

pp. 540-543

Author(s):

I. Tomášková ◽

J. Vítámvás ◽

J. Korecký

Keyword(s):

Czech Republic ◽

Picea Abies ◽

Pinus Sylvestris ◽

Tree Species ◽

Abiotic Factors ◽

Forest Tree ◽

Germination Capacity ◽

The Czech Republic ◽

Germination Energy ◽

Seed Ageing

:Germination capacity and germination energy are usually the most frequently used quantitative parameters of forest tree seed. With seed ageing both parameters decreased and the rate of the collapse is given by tree species, age of tree and its seed and biotic and abiotic factors. Relatively little attention has been paid to the age of seed. As it was found, the longevity of the main tree species remained relatively high, and spruce (Picea abies [L.] Karsten and pines (Pinus sylvestris L.) from the investigated areas across the Czech Republic maintained minimally one third of germination capacity or germination energy during the 10 years with the exception of larch (Larix decidua Mill.) where germination capacity decreased almost to zero after 10 years. Although the germination energy and germination capacity decreased significantly, it is possible to use the seed in the case of shortage of the seed of better quality.  

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Financial impact in case of investment to the forest property as a result of different tree species composition in the Czech Republic

E+M Ekonomie a Management ◽

10.15240/tul/001/2014-4-006 ◽

2014 ◽

Vol 17 (4) ◽

pp. 70-85

Author(s):

Pavlína Köhler ◽

Vlastimil Vala

Keyword(s):

Czech Republic ◽

Species Composition ◽

Tree Species ◽

Financial Impact ◽

Tree Species Composition ◽

The Czech Republic

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Models for predicting aboveground biomass of European beech (Fagus sylvatica L.) in the Czech Republic

Journal of Forest Science ◽

10.17221/100/2014-jfs ◽

2016 ◽

Vol 61 (No. 2) ◽

pp. 45-54 ◽

Cited By ~ 1

Author(s):

Vejpustková Monika ◽

Zahradník Daniel ◽

Čihák Tomáš ◽

Šrámek Vít

Keyword(s):

Czech Republic ◽

Fagus Sylvatica ◽

Aboveground Biomass ◽

European Beech ◽

The Czech Republic ◽

Fagus Sylvatica L

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Delineation of seed zones for European beech (Fagus sylvatica L.) in the Czech Republic based on isozyme gene markers

Annales des Sciences Forestières ◽

10.1051/forest:19980403 ◽

1998 ◽

Vol 55 (4) ◽

pp. 425-436 ◽

Cited By ~ 9

Author(s):

Dušan Gömöry ◽

Vladimír Hynek ◽

Ladislav Paule

Keyword(s):

Czech Republic ◽

Fagus Sylvatica ◽

European Beech ◽

Gene Markers ◽

The Czech Republic ◽

Seed Zones ◽

Fagus Sylvatica L

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Evaluation of changes in the tree species composition of Czech forests

Journal of Forest Science ◽

10.17221/4598-jfs ◽

2012 ◽

Vol 50 (No. 1) ◽

pp. 31-37 ◽

Cited By ~ 2

Author(s):

I. Tomášková

Keyword(s):

Czech Republic ◽

Species Composition ◽

Human Impact ◽

Tree Species ◽

Target Species ◽

Tree Species Composition ◽

The Czech Republic ◽

Spruce Stands

The tree species composition of forests in the Czech Republic has changed due to the human impact over the time. Nowadays, the idea of reducing the area with spruce stands and of increasing the area with broadleaved stands is widely discussed. The paper compares the target species composition with the presentone and creates four groups of areas with the largest and/or negligible differences between the target and the actual tree species composition using the Czech typology school.

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Above-ground net primary productivity in young stands of beech and spruce

Forestry Journal ◽

10.2478/v10114-011-0022-y ◽

2013 ◽

Vol 59 (3) ◽

Cited By ~ 4

Author(s):

Jozef Pajtík ◽

Bohdan Konôpka ◽

Róbert Marušák

Keyword(s):

Climate Change ◽

Primary Productivity ◽

Tree Species ◽

Net Primary Productivity ◽

Standing Stock ◽

European Beech ◽

Forest Tree ◽

Tree Species Composition ◽

Climate Conditions ◽

Resistant Species

AbstractOne of the expected consequences of climate change and its inherent phenomena to forest ecosystems is the gradual modification of their tree species composition (i.e. expansion of resistant species instead of less resistant ones). Climate change accompanied with increasing temperatures and a lack of precipitations may present a threat especially to spruce stands in the European part of the temperate zone. European beech is one of the possible forest tree species which might replace the potentially endangered spruce. In this paper, we observed, by using a combination of continual measurements and destructive whole-tree sampling, standing stocks of above-ground biomass (i.e. stem, branches, and foliage) and its annual net primary productivity (NPP) in naturally regenerated young stands of beech and spruce. We intentionally selected a site where the changing climate conditions are better suited to the ecological demands of beech rather than spruce (the species is dominant in the observed area). We recorded only small differences in the standing stock of stems of the beech, if based on tons per ha. However, this is in favor of spruce if based on cubic meters per ha. The largest difference between the species was found for the standing stock of foliage, spruce retained three times the biomass of beech. Also, beech allocated more carbohydrates to stem than spruce. On the other hand, we estimated nearly the same production of foliages and branches in both stands.

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Molecular analysis of temporal genetic structuring in pea (Pisum sativum L.) cultivars bred in the Czech Republic and in former Czechoslovakia since the mid-20th century

Czech Journal of Genetics and Plant Breeding ◽

10.17221/127/2011-cjgpb ◽

2012 ◽

Vol 48 (No. 2) ◽

pp. 61-73 ◽

Cited By ~ 5

Author(s):

J. Cieslarová ◽

M. Hýbl ◽

M. Griga ◽

P. Smýkal

Keyword(s):

Genetic Diversity ◽

Czech Republic ◽

Genetic Erosion ◽

Genetic Distances ◽

Vital Role ◽

Genetic Structuring ◽

The Czech Republic ◽

Germplasm Collections ◽

Marker Loci

Changes in genetic diversity of peas bred in the Czech Republic and in former Czechoslovakia since the mid-20th century were analysed using 38 molecular marker loci, including retrotransposons and microsatellites, differentiating a total of 84 alleles. Both marker types were comparably effective in revealing the genetic diversity, with a high correlation (r = 0.81), although the pairwise genetic distances of each marker type differed. In total, 175 accessions, selected from the Czech pea gene bank collection and representing the pea cultivars collected or bred in the country, were divided into three groups according to their date of sampling or variety registration. The first group contained 70 old cultivars and landraces collected prior to 1961. The second group contained 46 cultivars released from 1961 to 1980. The third group contained 59 cultivars released between 1981 and 2004. In spite of the decline in several diversity measures, differences in allele frequencies and even allele loss in three microsatellite loci were recorded over the 70-year period, while these differences between the groups were not statistically significant. In addition, genetic heterogeneity was detected in 29 accessions (15%). This indicates that although no genetic erosion could be observed since then, it is important to monitor the genetic diversity, furthermore it highlights the vital role of germplasm collections for the crop diversity conservation.

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Genetic diversity of sessile oak populations in the Czech Republic

Journal of Forest Science ◽

10.17221/99/2021-jfs ◽

2022 ◽

Vol 68 (No. 1) ◽

pp. 8-18

Author(s):

Jakub Dvořák ◽

Jiří Korecký ◽

Zuzana Faltinová ◽

Dagmar Zádrapová

Keyword(s):

Genetic Diversity ◽

Czech Republic ◽

Tree Species ◽

Deciduous Tree ◽

Boundary Line ◽

The Czech Republic ◽

Sessile Oak ◽

Seed Sources ◽

Forest Reproductive Material ◽

Reproductive Material

The sessile oak is a broadleaved tree species of great ecological and silvicultural importance. Oaks are the second most widespread deciduous tree species in the Czech Republic, and ongoing climate change negatively affects the abundant and often monocultural Norway spruce. Therefore, a proportional increase of more resilient tree species such as sessile oak has emerged. This study aimed to depict population genetic diversity when analysing 272 individuals from 10 subpopulations selected across the Czech Republic. Targeted populations were chosen based on the minimal expected human impact on the stand (presumably autochthonous stands). All individuals were genotyped using 18 polymorphic microsatellite markers (SSRs) assembled into two amplification multiplexes. The high discriminatory power of SSR markers was tested and confirmed by the probability of identity analysis. The genetic differentiation of the subpopulations was low yet significant, quantified by Wright’s F-statistics within the range from 0.012 to 0.029. Based on discriminant analysis of principal components (DAPC), we detected two populations with geographic genetic correlation (the 15th meridian east being a north-south boundary line) and one with a distinct genetic pattern. We assume that the population might previously be established from seed sources outside the Czech Republic. Moreover, to some extent, our findings advocate the legitimacy of the legislative rules for forest reproductive material (FRM) transfer.

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