scholarly journals Pollen Germination and Pollen Tube Elongation of Tomato (Lycopersicum esculentum L.) Regulated by Cell Wall Invertase through Sucrose Hydrolysis

2017 ◽  
Vol 7 (6) ◽  
Author(s):  
Astija
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Pollen Germination ◽  
Lycopersicum Esculentum ◽  
Cell Wall Invertase ◽  
Sucrose Hydrolysis ◽  
Pollen Tube Elongation

scholarly journals In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

2017 ◽  
Vol 32 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Natalia Georgieva ◽  
Ivelina Nikolova ◽  
Valentin Kosev ◽  
Yordanka Naydenova
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Culture Media ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
In Vitro Germination ◽  
Pisum Sativum L ◽  
Pollen Tube Elongation

The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

Effect of nickel on pollen germination and pollen tube length in Arabis alpina (Brassicaceae)

2016 ◽  
Vol 64 (4) ◽  
pp. 302 ◽  
Author(s):  
Dolja Pavlova
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Tube Length ◽  
Serpentine Soils ◽  
Nutritional Medium ◽  
Arabis Alpina ◽  
Pollen Tube Elongation ◽  
Germinated Pollen ◽  
Pollen Tube Length

In this work we studied and compared the toxic effect of nickel (Ni) on pollen germination and pollen tube length in Arabis alpina L. collected from serpentine and non-serpentine populations distributed in the Rila mountains, Bulgaria. Pollen grains were treated with prepared standard solutions of 100, 300, 500, and 700 μM Ni as NiCl2 in distilled water. A nutritional medium was also used to assess pollen germination. Nickel inhibited pollen germination and pollen tube elongation in both serpentine and non-serpentine plants. The percentage of germinated pollen in serpentine plants treated with Ni was higher than in non-serpentine plants but there was no difference in pollen tube elongation between groups. However, pollen tubes showed abnormalities such as coiling and swelling of the tip, or burst, and varied considerably among the samples. A complete break of pollen tube elongation is due to such abnormalities. Also, decreased pollen fertility in both populations was found. The plants from serpentines were less sensitive to (i.e. more tolerant of) elevated Ni concentrations commonly found in serpentine soils.

Roles for NO and ROS signalling in pollen germination and pollen-tube elongation in Cupressus arizonica

2015 ◽  
Vol 59 (4) ◽  
pp. 735-744 ◽  
Author(s):  
S. Pasqualini ◽  
M. Cresti ◽  
C. Casino ◽  
C. Faleri ◽  
G. Frenguelli ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Pollen Tube Elongation ◽  
Ros Signalling ◽  
Cupressus Arizonica

scholarly journals Physiological Importance of Pectin Modifying Genes During Rice Pollen Development

2020 ◽  
Vol 21 (14) ◽  
pp. 4840
Author(s):  
Yu-Jin Kim ◽  
Ho Young Jeong ◽  
Seung-Yeon Kang ◽  
Jeniffer Silva ◽  
Eui-Jung Kim ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Pollen Germination ◽  
Germination Rate ◽  
Expression System ◽  
Pectin Methylesterase ◽  
Distribution Patterns ◽  
Fine Tuning ◽  
Specific Expression

Although cell wall dynamics, particularly modification of homogalacturonan (HGA, a major component of pectin) during pollen tube growth, have been extensively studied in dicot plants, little is known about how modification of the pollen tube cell wall regulates growth in monocot plants. In this study, we assessed the role of HGA modification during elongation of the rice pollen tube by adding a pectin methylesterase (PME) enzyme or a PME-inhibiting catechin extract (Polyphenon 60) to in vitro germination medium. Both treatments led to a severe decrease in the pollen germination rate and elongation. Furthermore, using monoclonal antibodies toward methyl-esterified and de-esterified HGA epitopes, it was found that exogenous treatment of PME and Polyphenon 60 resulted in the disruption of the distribution patterns of low- and high-methylesterified pectins upon pollen germination and during pollen tube elongation. Eleven PMEs and 13 PME inhibitors (PMEIs) were identified by publicly available transcriptome datasets and their specific expression was validated by qRT-PCR. Enzyme activity assays and subcellular localization using a heterologous expression system in tobacco leaves demonstrated that some of the pollen-specific PMEs and PMEIs possessed distinct enzymatic activities and targeted either the cell wall or other compartments. Taken together, our findings are the first line of evidence showing the essentiality of HGA methyl-esterification status during the germination and elongation of pollen tubes in rice, which is primarily governed by the fine-tuning of PME and PMEI activities.

scholarly journals Phenolic Compounds Produced by Secretory Structures in Plants: A Brief Review

2008 ◽  
Vol 3 (8) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
M. Castro Marilia De ◽  
Diego Demarco
Keyword(s):  
Phenolic Compounds ◽  
Pollen Tube ◽  
Pollen Germination ◽  
Chemical Defence ◽  
Complex Mixtures ◽  
Secretory Cells ◽  
Vascular Bundles ◽  
Secretory Structures ◽  
Vegetative Organs ◽  
Pollen Tube Elongation

The purpose of this brief review has been to provide more recent data regarding the production of phenolic compounds by secretory structures. Although morphology and histochemistry of glands are well documented, meagre information concerning phenolics is available in the surveyed literature. Two major groups of glands are found regarding phenolic compounds synthesis: 1. secretory cells producing mainly phenolics, 2. secretory cells producing phenolics coupled with other compounds. In the former group, phenolic compounds remain in mature organs, and prevail in the material produced by epidermis, hypodermis, idioblasts, and sheath encircling vascular bundles and ducts. The latter group is constituted of trichomes, cavities, ducts, laticifers, colleters, nuptial nectaries, osmophores and stigma system, which synthesize complex mixtures of terpenes, phenolic compounds, polysaccharides and other compounds. In vegetative organs, the secretion of these glands might provide chemical defence against damage by UV radiation, against pathogen activities, and play a role in the herbivory deterrence. Additional functions ascribed to phenolics produced by floral glands are associated with pollination, pollen germination and pollen-tube elongation.

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