Evaluation of the Cytotoxic Effects of Ultrasonic Extracts of Tribulus Terrestris L. on MCF-7 Cell Line by MTT Assay

Background: Breast cancer is most frequently diagnosed cancer globally but there is not any ideal economical and safer agent that not only decreases the progression but also resolve complexities associated with breast cancer such as inflammatory conditions. There was strong link between inflammation and cancer specially breast cancer. Thus by inhibiting the COX enzyme may inhibit the progression of cancer beside of its role in inflammatory conditions of breast. Study Design: Interventional In Vitro trial. Setting: Department of Pharmacology in alliance with PCMD. Period: The duration of study from April 2016 to February 2017. Methodology: For this purpose we used five cancerous lines MCF-7, MDA-MB-231, MCF-10, HT-29 and Hela cell lines. For demonstrating the cytotoxic effects of Apricoxib we used MTT assay (for all cell Lines) and Trypan blue dye exclusion assay (Primarily for MCF-7 cell lines). For calculation of minimum dose required for exert cytotoxic effects of Apricoxib and its selectivitytowards cancerous cells of breast tissue we calculated its IC50 value and Selectivity Index (SI) by MTT assay. Results: Apricoxib significantly reduce the viability of MCF-7, MDA-MB-231, Hela, HT-29 as assessed by MTT assay in dose dependent manner (χ2 (2) = 26.483, p<0.001), (χ2 (2) = 26.49, p<0.001), (χ2 (2) = 26.062, p<0.001) and (χ2 (2) = 26.062, p<0.001) respectively. However Apricoxib had non-significant effects on % viability of MCF-10 cell line (χ2 (2) = 4.167, p=0.654) as assessed by MTT assay. Furthermore Apricoxib had lowest IC50 value against MCF-7 cell line. Conclusion: This study demonstrated that beside of primarily anti-inflammatory effects Apricoxib have additional benefits in term of exerting the cytotoxic effects (in vitro) on cancerous cell lines as indicated by reducing the % viability and reducing the Absorbance value of test sample as compare to control. This opens the newer path for researcher to evaluate different aspects of Apricoxib in field of chemotherapy.

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Synthesis of 1-[(Aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic Acid Derivatives and Their Breast Anticancer Activity

Crystals ◽

10.3390/cryst11050571 ◽

2021 ◽

Vol 11 (5) ◽

pp. 571

Author(s):

Ahmed Gaber ◽

Walaa F. Alsanie ◽

Majid Alhomrani ◽

Abdulhakeem S. Alamri ◽

Ibrahim M. El-Deen ◽

...

Keyword(s):

Breast Cancer ◽

Carboxylic Acid ◽

Anticancer Activity ◽

Cell Line ◽

Mtt Assay ◽

Analytical Techniques ◽

Anticancer Effect ◽

Reference Compound ◽

Anticancer Effects ◽

Mcf 7

This research aimed to produce new 1-[(aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic acid derivatives and check their anticancer effect against the breast cancer MCF-7 cell line. The 2-oxo-1,2-dihydroquinoline-3-carboxylic acid (4) compound was obtained by hydrolyzing ethyl 2-oxo-1,2-dihydroquinoline-3-carboxylate (2) with thiourea and anhydrous potassium carbonate ethanol, which was then treated with ethyl 3-substituted 2-cyanoacrylates (6) in the presence of triethylamine in diethyl formamide to give 1-[2-(ethoxy)carbonyl-2-cyano-1-arylvinyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic (7a,d). Cyclization of compound 7 with hydrazine hydrate ethanol inferred the association of 1-[(aryl)(3 amino-5-oxopyrazolidin-4-ylidene)methyl-2-oxo-1,2-dihydroquinol-3-carboxylates (8a,d). Spectroscopic and micro-analytical techniques such as IR, NMR, and elemental analysis were used to validate the structure of the synthesized organic compounds. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested by using the MTT assay on the MCF-7 cell line. When compared to the reference compound Dox, the compounds 7b, 7c, 8a, 8b, and 8c demonstrated strong anticancer activity against the MCF-7 cell line. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested against the MCF-7 cell line, using MTT assay. The compounds 7b, 7c, 8a, 8b, and 8c showed significant anticancer activity compared to the reference compound Dox against the MCF-7 cell line.

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Cytotoxic Constituents of Pachyrhizus Tuberosus from Peruvian Amazon

Natural Product Communications ◽

10.1177/1934578x1300801022 ◽

2013 ◽

Vol 8 (10) ◽

pp. 1934578X1300801 ◽

Cited By ~ 1

Author(s):

Olga Leuner ◽

Jaroslav Havlik ◽

Milos Budesinsky ◽

Vladimir Vrkoslav ◽

Jessica Chu ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Mtt Assay ◽

Human Cancer ◽

Chemical Constituents ◽

Human Fibroblasts ◽

Cancer Cell Lines ◽

Cytotoxic Effects ◽

Hct 116 ◽

Mcf 7

Investigations into the chemical constituents of the seeds of the neglected tuber crop Pachyrhizus tuberosus (Leguminosae) resulted in the isolation of seven components: five rotenoids [12a-hydroxyerosone (1), 12a-hydroxydolineone (2), erosone (3), 12a-hydroxyrotenone (4) and rotenone (6)], a phenylfuranocoumarin [pachyrrhizine (5)] and an isoflavanone [neotenone (7)]. The compounds were isolated using several chromatography techniques and characterized and verified by NMR and HPLC/MS. The MTT assay was used to examine the selective cytotoxic effects of the methanolic P. tuberosus extract and isolated compounds in two human cancer cell lines [breast (MCF-7) and colorectal (HCT-116)] and in non-transformed human fibroblasts (MRC-5); IC50 values were calculated. The methanolic P. tuberosus extract displayed respectable cytotoxic effects against HCT-116 and MCF-7 cells with IC50 values of 7.3 and 6.3 μg/mL, respectively. Of the compounds, 6 exacted greatest cytotoxicity and selectivity towards the cancer cell lines tested, yielding IC50 values of 0.3 μg/mL against both MCF-7 and HCT-116 cells, and a 6-fold reduced activity against MRC-5 fibroblasts. Compound 4 also demonstrated cytotoxicity against MCF-7 and HCT-116 (1.1 and 1.8 μg/mL, respectively), and reduced cytotoxicity towards MRC-5 cells (7.5 μg/mL). The results revealed from the in vitro cytotoxic MTT assay are worthy of further antitumor investigation.

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Antiradical Activities and Cytotoxic Effects of Phlomis Brevibracteata Turrill on SK-HEP-1 Adenocarcinoma Cell Line

Proceedings ◽

10.3390/proceedings2251575 ◽

2018 ◽

Vol 2 (25) ◽

pp. 1575

Author(s):

Imge Kunter ◽

Niloofar Zabib ◽

Kelly Randmae ◽

Mahshid Dabeshlim ◽

Muberra Kosar

Keyword(s):

Cell Line ◽

Mtt Assay ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Scavenging Activity ◽

Endemic Plant ◽

Cytotoxic Effects ◽

Phenolic Extract

The present study investigates the total phenol amount, radical scavenging and anticancer activity of methanolic extract of Phlomis brevibracteata Turrill (PBT) leaves; an endemic plant of North Cyprus. The antiradical activity and cytotoxicity of 70% methanolic extract of PBT leaves were analyzed by the implementation of DPPH, ABTS radical scavenging activity tests and MTT assay, respectively. Our results showed significant antiradical scavenging activity in the total phenolic content of the PBT leaves. MTT assay proved potent cytotoxic activity against SK-HEP-1 cell line not only for 70% methanolic extract of PBT, but also for gallic acid and caffeic acid constituents of the total phenolic extract.

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Modification of MTT assay conditions to examine the cytotoxic effects of amitraz on the human lymphoblastoid cell line, WIL2NS

Toxicology in Vitro ◽

10.1016/j.tiv.2005.05.001 ◽

2005 ◽

Vol 19 (8) ◽

pp. 1051-1059 ◽

Cited By ~ 65

Author(s):

Fiona M. Young ◽

Wichaya Phungtamdet ◽

Barbara J.S. Sanderson

Keyword(s):

Cell Line ◽

Mtt Assay ◽

Lymphoblastoid Cell Line ◽

Cytotoxic Effects ◽

Human Lymphoblastoid Cell ◽

Human Lymphoblastoid Cell Line ◽

Assay Conditions

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Correlation Between Antioxidant and Cytotoxic Activity of Parijoto (Medinilla speciosa Blume) Fractions in 4T1 Cell Line

Indonesian Journal of Cancer Chemoprevention ◽

10.14499/indonesianjcanchemoprev12iss1pp21-27 ◽

2021 ◽

Vol 12 (1) ◽

pp. 21

Author(s):

Eva Annisaa' ◽

Widyandani Sasikirana ◽

Nuraini Ekawati ◽

Intan Rahmania Eka Dini

Keyword(s):

Antioxidant Activity ◽

Cytotoxic Activity ◽

Cell Line ◽

Mtt Assay ◽

Ethyl Acetate Fraction ◽

Strong Positive Correlation ◽

Cytotoxic Effects ◽

Assay Methods ◽

Botanical Extracts ◽

Diphenyl Tetrazolium Bromide

Parijoto (Medinilla speciosa Blume) is one of Indonesian plant used for traditional medicine. Previous studies have demonstrated antimicrobial and cytotoxic effects of Parijoto on T47D cells. Therefore, we intended to know the antioxidant and cytotoxic activity of these fractions in 4T1 cell line (a Mus musculus mammary carcinoma). This cancer causes the greatest number of cancer-related deaths This study also investigated the correlation between antioxidant activity and cytotoxicity of Parijoto fractions. Discovering the type of correlation between antioxidant and anticancer activity of botanical extracts could relieve in screening for cytotoxic agent from natural products. The antioxidant and cytotoxic activity investigated using the Diphenylpicrylhydrazyl (DPPH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay methods. The result showed that ethyl acetate fraction is the higher antioxidant activity (IC50:1.77 μg/mL) and the higher cytotoxicity (IC50:133.57 μg/mL). There was a strong positive correlation (correlation coefficient=0.957) between antioxidant and cytotoxic activity in 4T1 cell line, but the correlation was not significant (p=0.188).Keywords: Parijoto (Medinilla speciosa Blume), antioxidant, cytotoxic, 4T1 cell line.

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Anticancer Activity of Silver Nan Particles against Cell Line MCF-7–Human - Breast Cancer through MTT Assay

10.4108/eai.7-12-2021.2314712 ◽

2021 ◽

Author(s):

C. K. Senthilkumaran ◽

S. Sugapriya

Keyword(s):

Breast Cancer ◽

Anticancer Activity ◽

Cell Line ◽

Mtt Assay ◽

Human Breast Cancer ◽

Human Breast ◽

Mcf 7

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Synergistic cytotoxic effects of arsenite and tetrandrine in human breast cancer cell line MCF-7

International Journal of Oncology ◽

10.3892/ijo.2017.4052 ◽

2017 ◽

Vol 51 (2) ◽

pp. 587-598 ◽

Cited By ~ 13

Author(s):

Mingjiang Yao ◽

Bo Yuan ◽

Xiao Wang ◽

Ai Sato ◽

Kana Sakuma ◽

...

Keyword(s):

Breast Cancer ◽

Cell Line ◽

Breast Cancer Cell ◽

Breast Cancer Cell Line ◽

Human Breast Cancer ◽

Cancer Cell Line ◽

Human Breast Cancer Cell ◽

Human Breast ◽

Cytotoxic Effects ◽

Mcf 7

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Comparison of Anticancer Activity of Dorycnium pentaphyllum Extract on MCF-7 and MCF-12A Cell Line: Correlation with Invasion and Adhesion

Biomolecules ◽

10.3390/biom11050671 ◽

2021 ◽

Vol 11 (5) ◽

pp. 671

Author(s):

Gözde Koygun ◽

Emine Arslan ◽

Gökhan Zengin ◽

Giustino Orlando ◽

Claudio Ferrante

Keyword(s):

Breast Cancer ◽

Lipid Peroxidation ◽

Cell Line ◽

Cytotoxic Effect ◽

Bioinformatics Analysis ◽

Biological Activities ◽

Xtt Assay ◽

Cytotoxic Effects ◽

Antiproliferative Effects ◽

Mcf 7

Dorycnium pentaphyllum subsp. haussknechtii is an important medicinal plant in several countries, including Turkey. This study aimed to evaluate the cytotoxicity of a crude extract of D. pentaphyllum subsp. haussknechtii against different breast cell lines to determine invasion, adhesion, and lipid peroxidation. The cytotoxic effects on MCF-7 breast cancer and MCF-12A as the immortalized cell line were examined by the XTT assay. Invasion and adhesion studies were performed according to the manufacturer’s kit procedure to IC50 values for 48 h. Lipid peroxidation was measured in the MCF-7 cell. A bioinformatics analysis was conducted to unravel the mechanism of action underlying antiproliferative effects, as well. According to XTT results, the tested extract showed a time- and a concentration-dependent cytotoxic effect. The most effective concentration was 100.5 µg/mL (48 h), which was selected for biological activities, such as apoptotic activity, invasion, adhesion, and lipid peroxidation assays. The extract caused tumoral cell death, and it did not have a cytotoxic effect on healthy human breast cells. Duplication times and measurement of CI analyses of cells were performed using the real-time cell analysis system xCELLigence. Finally, the bioinformatics analysis indicated the prominent role of quercetin as an extract component exerting a key role in the observed antiproliferative effects. This was supported by the micromolar/submicromolar affinity of quercetin towards proto-oncogene serine/threonine–protein kinase (PIM-1) and hematopoietic cell kinase (HCK), both involved in breast cancer. Altogether, our findings proposed that the extraction of the plant can be an effective strategy to isolate biomolecules with promising cytotoxic effects against breast cancer cells.

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