scholarly journals Effect of follicular ablation and gonadotropin priming on the recovery and quality of oocytes in Boran cows

2021 ◽  
pp. 138-143
Author(s):  
Demissie Tilaye ◽  
Yilma Tefera ◽  
Degefa Tamrat ◽  
Wirtu Gemechu ◽  
Lemma Alemayehu
Keyword(s):  
Corpus Luteum ◽  
Divided Dose ◽  
Recovery Rate ◽  
Oocyte Quality ◽  
Cleavage Rate ◽  
Exogenous Hormones ◽  
Maturation Rate ◽  
The Mean ◽  
Possible Cause

Genetic differences have been suggested as a possible cause for variation in responses to exogenous hormones. Here we evaluated the effect of follicle ablation, exogenous FSH and coasting time prior to ovum pick-up (OPU) on the number of follicles suitable for aspiration, oocyte quality, and cleavage rate in Ethiopian Boran cows. The experiment was carried out in three parts, I) Cows were synchronized using 500µg PGF2α given 11 days apart. Cows were then subjected to a biweekly ovum pickup session before ovulation (n=5) or starting Day 7 after ovulation (n=4) for three weeks. II) Cows were similarly synchronized and all visible follicles were ablated on the first days of overt estrus which were then further grouped into cows that received a divided dose of 350IU FSH (n=5) or 175IU FSH (n=5) over three days. In both groups OPU was carried out weekly starting 48h after the last FSH for six weeks. III) A similar protocol as in part II was carried out but coasting period was increased to 72hrs for cows that received 350IU FSH as divided dose (n=5) and 48hrs coasting period for single 350IU FSH dose (n=5). The covariates of follicles and oocyte were not affected (P>0.05) by corpus luteum presence at OPU. The mean number of medium (7.36±0.57) and large (8.28±0.96) follicles were significantly higher (P<0.05) in group that received divided 350IU FSH. Similarly, the mean number of Grade-1 (4.19±0.24) and Grade-2 (4.32±.27) COC, maturation rate (70.41%) and cleavage rate (47.5%) were significantly higher (P<0.05) in group that received 350IU FSH. COC quality was significantly (P<0.05) influenced by costing period. However, both maturation and cleavage rates were not affected by the coasting period. This study demonstrated that follicular ablation and treatment with FSH improves follicular population and oocyte recovery rate in Boran cows.

19 Invitro embryonic development from oocytes collected by ovum pickup of superstimulated females and nonstimulated slaughterhouse ovaries of alpaca (Vicugna pacos)

2021 ◽  
Vol 33 (2) ◽  
pp. 117
Author(s):  
L. Landeo ◽  
M. Zuñiga ◽  
T. R. Gastelu ◽  
J. A. Ruiz
Keyword(s):  
Embryonic Development ◽  
Statistical Significance ◽  
Calf Serum ◽  
Oocyte Quality ◽  
Blastocyst Stage ◽  
Cleavage Rate ◽  
Hormonal Stimulation ◽  
Array Transducer ◽  
The Mean

The objective of this study was to evaluate the embryonic development of alpaca oocytes collected by ovum pickup from superstimulated females (OPU, Group 1) and from slaughterhouse ovaries of 8 non-superstimulated females (SHO, Group 2) using a conventional aspiration technique (20G needle and a 3-mL syringe). A total of 8 nonpregnant alpacas, 3 to 4 years old, were superstimulated with a single dose of 200IU of equine chorionic gonadotrophin (eCG, Day=0). Three days later, alpacas were examined by transrectal ultrasonography with a 7.5-MHz linear-array transducer to determine the number and diameter of follicles available for aspiration. A total of 101 follicles were aspirated, recovering 67 oocytes (66.3%) by OPU using an endocavity transducer attached to a 21G needle adapted for alpacas. The follicular fluid was aspirated using a regulated vacuum pump (40 mmHg) into a tube containing 5mL of phosphate-buffered saline (PBS), 0.2% bovine serum albumin (BSA), and 10IUmL−1 heparin, at 37°C. In the SHO group we used 16 ovaries maintained at 28°C. The recovery of oocytes was carried out within 3h of ovary collection. We aspirated 155 follicles from SHO and recovered 117 oocytes (75.5%). After collection, all oocytes recovered were morphologically classified into categories (I and II) and cultured for 26h in an incubator (5% CO2 in air at 38.5°C), in TCM-199 supplemented with 0.2mmol sodium pyruvate, 50µgmL−1 gentamicin sulphate, 0.02IUmL−1 FSH, 1µgmL−1 oestradiol 17β, and 10% fetal calf serum (FCS). After maturation, oocytes were invitro fertilized with epididymal spermatozoa recovered from postmortem males and co-cultured for 18 to 20h. After this period, all cleaved oocytes were incubated (5% CO2 in air, 38.5°C) for 6 days in synthetic oviductal fluid-serum medium. Number and morphological quality of oocytes collected, invitro cleaved, and embryos ratea were registered and compared between groups. Statistical significance was determined using Kruskal–Wallis test. The mean and standard error were calculated from average of the percentages obtained in each repetition. Results indicated that the mean number of oocytes collected per ovary was higher (P&lt;0.05) using SHO (7.8±2.4) than OPU (4.5±3.0). Also, the number of oocytes classified as category I, was higher in the SHO compared with OPU group (56% vs. 30% respectively; P&lt;0.05); however, category II oocytes were the same (16% vs. 15%, respectively). There was no difference in early development (cleavage) rate between OPU (57±2.0) and SHO (49±1.5) groups. However, there was difference in the rate of development (P&lt;0.05) between OPU and SHO groups to reach the morula stage (56±2.0 vs. 42±1.7, respectively) and early blastocyst stage (55±2.0 vs. 34±1.4, respectively). In conclusion, oocyte quality could be affected by hormonal stimulation or by the quality of follicles aspirated by OPU. In contrast, oocytes recovered from live animals by OPU have greater capability of embryonic development invitro than oocytes recovered from slaughterhouse ovaries.

277 FOLLICULAR GROWTH, SUBSEQUENT OVUM PICKUP, AND DOMINANT FOLLICLE REMOVAL IN COWS

2006 ◽  
Vol 18 (2) ◽  
pp. 246
Author(s):  
K. Imai ◽  
M. Tagawa ◽  
S. Matoba ◽  
M. Narita ◽  
K. Kanayama
Keyword(s):  
Formation Rate ◽  
Cumulus Cell ◽  
Oocyte Quality ◽  
Developmental Competence ◽  
Dominant Follicle ◽  
Cleavage Rate ◽  
Significant Difference ◽  
The Mean ◽  
Follicle Aspiration ◽  
Removal Group

The present study was designed to assess the recruitment of follicles after ovum pickup (OPU) and dominant follicle (DF) removal on the follicular wave after OPU in Holstein dry cows. Cows were reared under the same feeding and environmental conditions. In Experiment 1, follicle aspiration (>2 mm in diameter) by OPU using a 7.5-MHz linear transducer with needle (COVA needle; Misawa Medical, Tokyo, Japan) connected to an ultrasound scanner (SSD-1200; ALOKA, Tokyo, Japan) was performed in four cows. Then, ovaries were observed after OPU from Day 1 (Day 0 = the day of OPU) to Day 11 to assess the number of follicles developed. In Experiment 2, two sessions of OPU were performed with a 7 day interval between sessions, with or without dominant follicle removal, to assess the quality of developing follicles and oocytes. In the DF removal group, >8-mm follicles were aspirated at Day 5 after the first OPU session, and the same cows without DF removal were designated as a control (n = 4, crossover trial). Oocytes were evaluated by their cumulus cell morphology, cytoplasmic color, and density. To assess the developmental competence of oocytes, Grades 1 and 2 cumulus-oocyte complexes (COCs) were collected, matured, fertilized, and cultured as described by Imai et al. (2002 J. Vet. Med. Sci. 64(10), 887-891). Embryo development was assessed by the cleavage rate on Day 2 and the blastocyst formation rate on Days 7 to 9 (the day of insemination = Day 0). Data were analyzed by ANOVA or Student t-test. In Experiment 1, a dominant follicle (>8 mm in diameter) was developed during Days 3 to 5 after OPU in each donor. The mean number of developing follicles (>2 mm in diameter) were increased from Day 1 to Day 9 (Day 1: 7.5 � 2.1, Day 3: 19.0 � 1.2, Day 5: 23.3 � 9.0, Day 7: 30.3 � 11.0, Day 9: 42.0 � 15.8 and Day 11: 41.0 � 16.7 (mean � SD), P < 0.05). In Experiment 2, there was no difference in the mean number of developing follicles on the day of OPU and collected oocytes between DF removal and control groups (follicles: 47.8 � 23.0 and 39.3 � 6.2; oocytes: 27.0 � 11.6 and 26.5 � 5.4, respectively). The number of Grades 1 and 2 oocytes for the DF removal group was significantly higher (P < 0.05) than that for the control (83.6 � 1.5 and 63.2 � 14.2, respectively), and no significant difference was found within cleavage (60.0 � 37.2, 53.6 � 23.2) and blastocyst rates (34.1 � 33.9, 34.4 � 16.8). These results indicate that populations of follicles were increased till Day 9 after OPU, and the DF removal was effective at increasing oocyte quality in the developing follicles.

206 EFFECT OF FOLLICULAR WAVE SYNCHRONIZATION AND SUPERSTIMULATION ON IN VITRO EMBRYO PRODUCTION

2008 ◽  
Vol 20 (1) ◽  
pp. 182 ◽  
Author(s):  
K. Imai ◽  
Y. Inaba ◽  
H. Yoshioka ◽  
Y. Aikawa ◽  
M. Ohtake ◽  
...  
Keyword(s):  
Formation Rate ◽  
Cumulus Cell ◽  
Oocyte Quality ◽  
Annual Conference ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Follicular Wave ◽  
The Mean ◽  
In Vitro Embryo Production

We previously reported that follicular wave synchronization, by removal of the dominant follicle on Day 5 after ovum pickup (OPU), was effective in increasing oocyte quality in the developing follicles (Imai et al. 2006 32th Annual Conference of the IETS, poster presentation no. 277). The current study was designed to examine the effect of superstimulatory treatment to induce subsequent follicular wave synchronization on embryo production by OPU and IVM-IVF-IVC in Holstein dry cows. Cows were reared under the same feeding and environmental conditions, and 2 OPU sessions were conducted in each cow. In the first session, OPU was performed in 8 cows on arbitrary days of the estrous cycle by using a 7.5-MHz linear transducer with needle (Cova needle, Misawa Medical, Tokyo, Japan) connected to an ultrasound scanner (SSD-1200, Aloka, Tokyo, Japan). Follicles larger than 8 mm in diameter were then aspirated and a CIDR was inserted on Day 5 (the day of first OPU session = Day 0). Cows then received 30 mg of FSH (Antrin-R10; Kawasaki Mitaka Pharmaceutical Co., Tokyo, Japan) twice a day from Days 7 to 10 in decreasing doses (6, 6, 4, 4, 3, 3, 2, 2 mg) by i.m. injection. Cloprostenol (PGF; Clopromate C; Sumitomo Pharmaceuticals Co., Tokyo, Japan; 0.75 mg) was administered in the morning of Day 9 (third day of superstimulation). The second OPU session was performed 48 h after PGF administration (Day 11), and only follicles larger than 5 mm in diameter were aspirated. The CIDR was removed from the cows just before OPU. Collected oocytes were evaluated by their cumulus cell morphology, cytoplasmic color, and density. Grades 1 and 2 COC were matured, fertilized, and cultured as described by Imai et al. [2006 J. Reprod. Dev. 52(Suppl.), S19–S29]. Embryo development was assessed by the cleavage rate on Day 2 and by the blastocyst formation rate on Days 7 to 8 (the day of insemination = Day 0). Data were analyzed by Student's t-test. There were no differences in the mean (� SD) number of aspirated follicles or collected oocytes between the first (32.5 � 6.8 and 26.0 � 12.7, respectively) and second (29.3 � 10.4 and 19.0 � 9.4, respectively) OPU sessions (P > 0.1). The percentage of Grade 1 and 2 oocytes for the second OPU session (90.5 � 13.8%) was significantly higher (P < 0.01) than for the first OPU session (63.1 � 6.3%), and significant differences were found for cleavage (79.4 � 14.1, 61.8 � 25.1, P < 0.01) and blastocyst rates (68.1 � 16.7, 24.2 � 22.3, P < 0.001) between sessions. The mean numbers of blastocysts obtained per session were 4.3 � 2.9 and 12.8 � 8.7 in the first and second sessions, respectively (P < 0.01). These results indicate that superstimulatory treatment and subsequent follicular wave synchronization were effective on in vitro embryo production by increasing the oocyte quality.

193 Effect of clinical metritis on oocyte recovery, oocyte quality, and early invitro developmental competence of embryos in Bos indicus dairy cattle

2020 ◽  
Vol 32 (2) ◽  
pp. 225
Author(s):  
M. Saleem ◽  
Z. Sarwar ◽  
M. Saad ◽  
I. Zahoor ◽  
N. Ahmad ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Recovery Rate ◽  
Body Condition Score ◽  
Bos Indicus ◽  
Oocyte Quality ◽  
Developmental Competence ◽  
Cleavage Rate ◽  
Healthy Group ◽  
Oocyte Recovery ◽  
Frozen Semen

Unhygienic practices at the time of parturition or AI lead to uterine infections. The uterine infections ultimately result in genetic drain by culling the elite animals. The invivo developmental competence of embryos is compromised in clinically metritic animals. The genetic potential of problematic females could be harvested by invitro embryo production (IVEP). Therefore, the objective of the present study was to evaluate the effect of clinical metritis on oocyte recovery, oocyte quality, and early invitro developmental competence of embryos in Bos indicus dairy cattle. This experiment was carried out from December 2017 to April 2018. Ovaries were collected from a local abattoir (Bos indicus; 5- to 8-year-old dairy cattle, body condition score 2.75±0.25, mixed parity). These ovaries (n=982) were divided into two groups: (1) clinically metritic (n=184), and (2) healthy (n=798), based upon the presence or absence of pus in the uterine lumen. Oocytes were aspirated from follicles using an 18G needle attached to a 10-mL syringe. Cumulus-oocyte complexes (COCs) were categorized into A, B, C, and D grades based on the number of layers of cumulus cells and integrity of ooplasm. The oocytes of grades A and B were subsequently transferred in groups (10/group) in four-well plates containing 100-μL droplets. The droplets with oocytes were covered with prewarmed mineral oil and incubated for 24h at 38.5°C, 5% CO2, and 95% relative humidity. The oocytes were evaluated for IVM on the basis of cumulus expansion. Frozen semen was thawed and prepared using the sperm swim-up procedure for each group. Spermatozoa and oocytes were incubated together for a period of 18h. The presumptive zygotes were invitro cultured for 4 days in a CO2 incubator under similar culture conditions. The cleavage rate, 4-cell, and 8-cell stages were recorded on Days 2, 3, and 4 after the day of insemination, respectively. Data on oocyte recovery, oocyte quality, IVM, cleavage rate, and 4-cell and 8-cell stages were analysed by Chi-squared test using SPSS software (version 20; IBM Corp.) for Windows. Results demonstrated that recovery rate was lower (63.8% vs. 71.7%; P&lt;0.05) in clinically metritic compared with healthy cattle. Similarly, oocytes of grade A and B quality were lower (41.0% vs. 51.1%; P&lt;0.05), whereas those of C and D quality were higher (59.0% vs. 48.9%; P&lt;0.05) in clinically metritic compared with the healthy group. Moreover, 4-cell (38.2% vs. 54.8%) and 8-cell stage embryos (11.3% vs. 29.1%), were lower (P&lt;0.05) in the clinically metritic compared with the healthy group, respectively. However, maturation rate and cleavage rate did not differ (P&gt;0.05) between groups. In conclusion, metritis in slaughterhouse ovaries negatively affects oocyte recovery rate, oocyte quality, and early invitro developmental competence of embryos in Bos indicus dairy cattle.

scholarly journals Comparative seasonal assessment on the quality of Black Bengal goat oocytes in view of in vitro embryo production

2020 ◽  
Vol 5 (3) ◽  
pp. 78-87
Author(s):  
Shikha Sarkar ◽  
Md Saiful Islam ◽  
Md Enayet Kabir ◽  
Falguni Dadok ◽  
Md Abdullah Al Zaber ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Recovery Rate ◽  
In Vitro Maturation ◽  
Winter Season ◽  
Excellent Opportunity ◽  
Different Seasons ◽  
In Vitro Embryo Production ◽  
Black Bengal Goat

In vitro maturation (IVM) of oocytes provide an excellent opportunity for cheap and abundant embryos for carrying out animal improvement. With the aim for studying the quality of oocyte in different seasons of Black Bengal goat, both right and left ovaries were collected from the slaughter houses. For each of the specimens, gross parameters such as right, left, corpus luteum (CL)-present and corpus luteum (CL)-absent group were evaluated on the basis of weight (g), length (cm), width (cm), follicles aspirated and number and state of cumulus-oocyte-complexes (COCs), normal COCs and abnormal COCs, the effect of season on cumulus oocyte complexes (COCs) of goat oocytes. Our study revealed that the average number of follicles and oocytes recovery rate were higher in summer than in winter season and recovery rate of grade A and B oocytes were higher in summer than that of in winter and recovery rate of grade C and D were higher in winter than that of in summer. In the present study a significantly greater number of oocytes per ovary were recovered from ovaries without a corpus luteum than from ovaries with a corpus luteum. So the summer was the best season for recovery of COCs which have a worthy competence to be matured in vitro. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (3), 78-87

143 Effect of corpus luteum on recovery rate, quality, and in vitro developmental competence of oocytes in Bos indicus dairy cows

2019 ◽  
Vol 31 (1) ◽  
pp. 196
Author(s):  
M. Saad ◽  
Z. Sarwar ◽  
M. Saleem ◽  
U. Arshad ◽  
M. Shahzad ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Corpus Luteum ◽  
Recovery Rate ◽  
Bos Indicus ◽  
Developmental Competence ◽  
Serum Concentrations ◽  
Embryo Production ◽  
The Mean ◽  
In Vitro Embryo Production

Assisted reproductive technologies have modernized the dairy industry for the rapid multiplication of superior genetic traits. However, the exploitation of genetics through in vitro embryo production in farm animals has been struggling during the last 3 decades. The presence or absence of a corpus luteum (CL) on ovaries from which cumulus-oocyte complexes were recovered has been shown to affect the quality of oocytes and in vitro embryo production outcome in Bos taurus cows. Similar information is lacking in Bos indicus cows. Therefore, the objectives of the present study were to determine the effect of presence or absence of a CL on recovery rate, quality, and in vitro developmental competence of oocytes in Bos indicus dairy cows. This study was conducted from December 2017 to April 2018. The ovaries were harvested from a local abattoir (Bos indicus; 5- to 8-year-old cows having mixed parity with clinically normal reproductive tracts). These ovaries (n=750) were divided into 2 groups [(1) CYCLIC (n=318) and (2) ACYCLIC (n=432)] based on the presence or absence of mature CL having follicles on either left or right ovaries of slaughtered cows. Blood samples from the jugular vein were collected at slaughter. Serum concentrations of progesterone of CYCLIC and ACYCLIC cows were measured using radioimmunoassay. Mean number of recovered oocytes per ovary and serum concentration of progesterone were compared using PROC TTEST model. However, quality, maturation, cleavage, 8-cell, 16-cell, and morula rates were analysed by PROC FREQ model of Chi-squared. All the data was analysed using SAS (SAS Institute Inc., Cary, NC, USA). Results revealed that the mean serum concentrations of progesterone (4.21±0.4v. 0.5±0.2ng mL−1; P&lt;0.05) were higher in CYCLIC as compared with ACYCLIC cows, respectively. The mean number of oocytes recovered per ovary (6.5±4.5v. 4.0±4.4; P&lt;0.05) was higher in CYCLIC than in ACYCLIC cows. The oocytes with grade I+II quality (55.3v. 47.6%; P&lt;0.05) were higher, whereas oocytes with grade III+IV quality (44.5v. 52.4%; P&lt;0.05) were lower, in CYCLIC as compared with ACYCLIC cows, respectively. Furthermore, cleavage rate (70.9v. 52.8%; P&lt;0.05) was higher in CYCLIC than in ACYCLIC cows, respectively. Similarly, the 8-cell (38.5v. 20.8%; P&lt;0.05) and 16-cell (20.0v. 10.9%; P&lt;0.05) stage embryos were greater in CYCLIC as compared with ACYCLIC cows, respectively. In conclusion, the presence of CL has a beneficial effect on recovery rate, quality, and in vitro embryo production outcomes in Bos indicus dairy cows. It is implied that for ovum pickup, cyclic cows should be preferred.

The effects of in vitro maturation duration on maturation rate and intracytoplasmic sperm injection-derived embryonic development in goats

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
A. H. Nor Farizah ◽  
M. M. Rahman ◽  
W. E. Wan Khadijah ◽  
R. B. Abdullah
Keyword(s):  
Embryonic Development ◽  
Intracytoplasmic Sperm Injection ◽  
Cumulus Cell ◽  
Cleavage Rate ◽  
Maturation Rate ◽  
Cell Layers ◽  
Embryo Cleavage

The aim of the present study was to evaluate the effects of <italic>in vitro</italic> maturation (IVM) duration on maturation rate and intracytoplasmic sperm injection (ICSI)-derived embryonic development in goat embryos. Donor goats were superovulated following oestrus synchronisation prior to laparoscopic oocyte pick-up. The quality of oocytes was scored based on cumulus cell layers, which were graded A, B and C. The oocytes were cultured in IVM medium with two different IVM durations (18-21 h and 22-25 h) for the ICSI procedure. A total of 327 matured oocytes were used, of which 157 and 170 oocytes were used for 18-21 h and 22-25 h IVM duration, respectively. The embryo cleavage rate of Grade A from the 18-21 h IVM group was significantly (P<0.05) higher than that of grades B and C. However, in the 22-25 h IVM duration group, the cleavage rates for all grades of oocytes were not significantly (P>0.05) different. Regardless of oocyte grades, no significant differences in maturation rates and cleavage rates for all stages of embryonic development between the two groups of IVM durations were observed. The results suggest that both IVM durations have the same potential in ICSI-derived embryonic development.

scholarly journals Effect of ovarian types and collection techniques on the number of follicles and the quality of cumulus-oocyte-complexes in cow

2017 ◽  
Vol 45 (3) ◽  
pp. 10-16
Author(s):  
Khandoker MAMY ◽  
NF Atiqah ◽  
N Ariani
Keyword(s):  
Corpus Luteum ◽  
Recovery Rate ◽  
Normal Basis ◽  
Blunt Dissection ◽  
Embryo Production ◽  
Aspiration Technique ◽  
In Vitro Embryo Production ◽  
Dissection Technique

This experiment was conducted for evaluation of bovine slaughterhouse ovary, follicles, and cumulus-oocyte-complexes (COCs) and to compare the effect of collection techniques on the recovery rate of COCs.  The collected slaughterhouse ovaries was classified as corpus luteum present (CL+) and corpus luteum absent (CL-) groups.  It was found that 62.5% of the ovaries collected were CL- type and 37.5% were CL+ type.  For collection of COCs, blunt dissection and aspiration techniques were performed and number of follicles collected was recorded.  The higher number of follicles were dissected and aspirated from CL - ovaries (11.2±1.8and 37.8±14.9, respectively) compared to CL+ ovaries 10.5±1.5and 28.3±15.6, respectively).  The follicular materials collected from both techniques were observed under microscope to categorize the COCs as A (oocyte surrounded with cumulous cells homogenously), B (oocyte surrounded with cumulous cells partially), C (oocyte not surrounded at all by cumulous cells) and D (degeneration observed both in oocyte and cumulous cells).  Grade A and grade B were classified as normal and grade C and grade D were considered as abnormal COCs.  The result indicated that ovaries having no CL contributing more total number of COCs per ovary (6.8±1.0) and also contributing higher normal COCs (5.7±0.9) than that of ovaries with CL (6.0±2.0 and 4.5±1.5, respectively) in blunt dissection technique.  But same trend of result was not found in aspiration technique.  Similarly, higher percentage of COCs recovery rate was also recorded in blunt dissection (61.6±4.6% vs 16.5±4.9%, on total basis) than aspiration (48.6±2.9% vs 11.7±4.1%, on normal basis) technique.  So, ovaries without CL and blunt dissection technique found more suitable for harvesting the higher number and superior quality of COCs for extending the in vitro embryo production experiment.Bang. J. Anim. Sci. 2016. 45 (3): 10-16

scholarly journals Supplementation of cows with linseed: effects on antral follicle count, oocyte quality, and nutritional and plasma metabolite parameters

2019 ◽  
Vol 40 (6) ◽  
pp. 2637
Author(s):  
Christopher Junior Tavares Cardoso ◽  
Ana Caroline Bini de Lima ◽  
Wilian Aparecido Leite da Silva ◽  
Mirela Brochado Souza-Cáceres ◽  
Daniela Moraes Pereira ◽  
...  
Keyword(s):  
Transvaginal Ultrasound ◽  
Antral Follicle ◽  
Oocyte Quality ◽  
Antral Follicle Count ◽  
Neutral Detergent Fiber ◽  
Significant Difference ◽  
Lipid Supplementation ◽  
The Mean ◽  
Plasma Metabolite

Linseed is distinguished by its composition of fatty acids, which are rich in unsaturated acids, and by the slow release of oil in the rumen, consequently providing a greater amount of lipids of interest for intestinal absorption. The aim of this study was to evaluate the effect of linseed supplementation on the antral follicle count (AFC) and oocyte quality of Girolando cows, as well as their digestive parameters and plasma metabolite. Twelve Girolando cows were used in the study and were randomly distributed in two experimental groups: control (CTL, n = 6) and linseed (LINS, n = 6). During the 126 days of supplementation, seven transvaginal ultrasound-guided ovum pick-up (OPU) sessions were performed at intervals of 21 days. AFC was performed in each OPU session. The cows fed with linseed showed no significant difference in the dry matter coefficient of digestibility (63.11 and 62.88), crude protein (62.7 and 55.26), neutral detergent fiber (NDF; 75.36 and 77.15), or acid detergent fiber (ADF; 72.45 and 74.77) for LINS and CTL, respectively. On the contrary, a higher level of ethereal extract was observed for LINS (69.31 vs. 40.7). There was no effect (p > 0.05) of lipid supplementation on plasma metabolite between groups and OPU sessions. There was no difference (p > 0.05) in the mean number of antral follicles (CTL: 31.14 ± 1.97; LINS: 25.52 ± 2.68), cumulus oocyte complexes recovered (CTL: 6.45 ± 1.66; LINS: 5.28 ± 1.18), or oocyte quality (CTL: 60.48% ± 8.46; LINS: 64.54% ± 7.77). The supplementation of 800g of linseed in the diet of Girolando cows did not alter the apparent digestibility of nutrients, AFC, or quality of oocytes obtained by OPU.

scholarly journals 246 FOLLICULAR DEVELOPMENT AND OOCYTE QUALITY AFTER OVUM PICKUP IN DONOR COWS

2005 ◽  
Vol 17 (2) ◽  
pp. 273
Author(s):  
K. Imai ◽  
M. Tagawa ◽  
S. Matoba ◽  
M. Narita ◽  
N. Saito
Keyword(s):  
Formation Rate ◽  
Calf Serum ◽  
Cumulus Cell ◽  
Follicular Development ◽  
Oocyte Quality ◽  
Developmental Competence ◽  
Cleavage Rate ◽  
The Mean ◽  
Follicle Aspiration ◽  
Student’S T

The present study was designed to assess the renewal of follicular development and oocyte quality after ovum pickup (OPU) in Holstein dry cows. Cows were kept under the same feeding and environmental conditions. In Experiment 1, follicle aspiration (more than 2 mm) by OPU using a 7.5 MHz linear transducer with needle (cova needle, Misawa Medical, Tokyo, Japan) connected to an ultrasound scanner (SSD-1200, ALOKA, Tokyo, Japan) was performed in four cows. After OPU ovaries were observed from Day 4 (Day 0 = the day of OPU) to Day 11 to assess the number of follicles that developed. In Experiment 2, two sessions of OPU (n = 11) were performed with a 7-day interval between to assess the quality of developing follicles and oocytes. Oocytes were evaluated by their cumulus cell morphology, cytoplasmic color, and density. To assess the developmental competence of oocytes, collected cumulus-oocyte complexes (COCs) were cultured for 20 h in TCM-199 supplemented with 5% calf serum (CS) in a microdroplet (volume was adjusted to 5 μL/oocyte) at 38.5°C under atmosphere of 5% CO2 in air. After maturation, the COCs were inseminated with frozen-thawed semen collected from the same ejaculation of a single bull. The fertilization was performed with BO solution as described by Imai et al. (2002 J. Vet. Med. Sci. 64(10), 887–891). The putative zygotes were then cultured in CR1aa supplemented with 5% CS under the same conditions as maturation culture for nine days. Embryo development was assessed by the cleavage rate on Day 2 and the blastocyst formation rate on Days 7 to 9 (the day of insemination = Day 0). Data were analyzed by ANOVA or Student's t-test. In Experiment 1, the mean number of developing follicles (larger than 2 mm in diameter) were increased from Day 4 to Day 11 (Day 4: 19.8 ± 10.0, Day 7: 32.5 ± 9.5; Day 11: 39.5 ± 10.7 (mean ± SD), respectively, P < 0.05). In Experiment 2, the mean number of developing follicles and collected oocytes on the day of OPU were significantly (P < 0.05) different between the first and second sessions (54.2 ± 12.4 and 40.8 ± 12.7, 45.7 ± 20.2 and 27.7 ± 8.7, respectively). The percentage of Grade 1 and 2 oocytes for the first session was significantly lower (P < 0.05) than those for the second session (59.1 ± 8.4 and 69.0 ± 11.8), and no significant differences were found within cleavage and blastocyst rates. The mean numbers of blastocysts obtained per session were 14.2 ± 8.9 and 9.7 ± 6.3 in the first and second sessions, respectively. These results indicate that populations of follicles were increased till Day 11 after OPU, and proportion of normal oocytes were increased in the renewal follicles.

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