scholarly journals Characterization of Clove Oil Nanoparticles and Their Insecticidal Activity against Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae)

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Silvi Ikawati ◽  
Toto Himawan ◽  
Abdul Latief Abadi ◽  
Hagus Tarno
Keyword(s):  
Insecticidal Activity ◽  
Clove Oil ◽  
Cryptolestes Ferrugineus

Isolation and characterization of clove oil from clove leaves (Syzygiumaromaticum) and its application as anti-bacterial

2021 ◽  
Author(s):  
Nini Munirah Renur ◽  
Sutrisno ◽  
Emmy Darmawati ◽  
Nurjanah
Keyword(s):  
Clove Oil ◽  
Isolation And Characterization

Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L.

2014 ◽  
Vol 30 (10) ◽  
pp. 2655-2662 ◽  
Author(s):  
Zhizhen Pan ◽  
Lian Xu ◽  
Yujing Zhu ◽  
Huai Shi ◽  
Zheng Chen ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity ◽  
Plutella Xylostella

scholarly journals Larvicidal Activity against Aedes aegypti and Chemical Characterization of the Inflorescences of Tagetes patula

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Letícia Maria Krzyzaniak ◽  
Tânia Mara Antonelli-Ushirobira ◽  
Gean Panizzon ◽  
Ana Luiza Sereia ◽  
José Roberto Pinto de Souza ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Insecticidal Activity ◽  
Chemical Characterization ◽  
Reversed Phase ◽  
Acetone Extract ◽  
Tagetes Patula ◽  
Larval Population

The crude acetone extract (CAE) of defatted inflorescences of Tagetes patula was partitioned into five semipurified fractions: n-hexane (HF), dichloromethane (DF), ethyl acetate (EAF), n-butanol (BF), and aqueous (AQF). BF was fractionated by reversed-phase polyamide column chromatography, obtaining 34 subfractions, which were subjected to HSCCC, where patuletin and patulitrin were isolated. CAE and the fractions BF, EAF, DF, and AQF were analyzed by LC-DAD-MS, and patuletin and patulitrin were determined as the major substances in EAF and BF, respectively. BF was also analyzed by HPLC and capillary electrophoresis (CE), and patulitrin was again determined to be the main substance in this fraction. CAE and the semipurified fractions (750, 500, 300, 100, and 50 mg/L) were assayed for larvicidal activity against Aedes aegypti, with mortality rate expressed as percentage. All fractions except AQF showed insecticidal activity after 24 h exposure of larvae to the highest concentration. However, EAF showed the highest activity with more than 50% reduction in larval population at 50 mg/L. The insecticidal activity observed with EAF might have been due to the higher concentration of patuletin present in this fraction.

scholarly journals Insecticidal Activity of Bacteria from Larvae Breeding Site with Natural Larvae Mortality: Screening of Separated Supernatant and Pellet Fractions

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 486 ◽  
Author(s):  
Handi Dahmana ◽  
Didier Raoult ◽  
Florence Fenollar ◽  
Oleg Mediannikov
Keyword(s):  
Biological Control ◽  
Insecticidal Activity ◽  
Breeding Site ◽  
Bacterial Strains ◽  
Activity Prediction ◽  
Pcr Screening ◽  
Breeding Sites ◽  
Insecticide Activity ◽  
New Compounds

Mosquitoes can transmit to humans devastating and deadly pathogens. As many chemical insecticides are banned due to environmental side effects or are of reduced efficacy due to resistance, biological control, including the use of bacterial strains with insecticidal activity, is of increasing interest and importance. The urgent actual need relies on the discovery of new compounds, preferably of a biological nature. Here, we explored the phenomenon of natural larvae mortality in larval breeding sites to identify potential novel compounds that may be used in biological control. From there, we isolated 14 bacterial strains of the phylum Firmicutes, most of the order Bacillales. Cultures were carried out under controlled conditions and were separated on supernatant and pellet fractions. The two fractions and a 1:1 mixture of the two fractions were tested on L3 and early L4 Aedes albopictus. Two concentrations were tested (2 and 6 mg/L). Larvae mortality was recorded at 24, 48 and 72 h and compared to that induced by the commercialized B. thuringiensis subsp. israelensis. Of the 14 strains isolated, 11 were active against the A. albopictus larvae: 10 of the supernatant fractions and one pellet fraction, and mortality increased with the concentration. For the insecticide activity prediction in three strains of the Bacillus cereus complex, PCR screening of the crystal (Cry) and cytolytic (Cyt) protein families characteristic to B. thuringiensis subsp. israelensis was performed. Most of the genes coding for these proteins’ synthesis were not detected. We identified bacterial strains that exhibit higher insecticidal activity compared with a commercial product. Further studies are needed for the characterization of active compounds.

Molecular and Morphological characterization of multiple nucleopolyhedrovirus from Mexico and their insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

2019 ◽  
Vol 144 (1-2) ◽  
pp. 123-132
Author(s):  
Magali Ordóñez‐García ◽  
Claudio Rios‐Velasco ◽  
José de Jesús Ornelas‐Paz ◽  
Juan Carlos Bustillos‐Rodríguez ◽  
Carlos Horacio Acosta‐Muñiz ◽  
...  
Keyword(s):  
Insecticidal Activity ◽  
Spodoptera Frugiperda ◽  
Morphological Characterization ◽  
Multiple Nucleopolyhedrovirus ◽  
Lepidoptera Noctuidae

Purification and Characterization of a High-Molecular-Weight Insecticidal Protein Complex Produced by the Entomopathogenic BacteriumPhotorhabdus luminescens

1998 ◽  
Vol 64 (8) ◽  
pp. 3029-3035 ◽  
Author(s):  
David J. Bowen ◽  
Jerald C. Ensign
Keyword(s):  
Molecular Weight ◽  
Insecticidal Activity ◽  
Protein Complex ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Protein Toxin ◽  
The Family ◽  
Toxin Complex

ABSTRACT Photorhabdus luminescens is a gram-negative enteric bacterium that is found in association with entomopathogenic nematodes of the family Heterorhabditidae. The nematodes infect a variety of soil-dwelling insects. Upon entering an insect host, the nematode releases P. luminescens cells from its intestinal tract, and the bacteria quickly establish a lethal septicemia. When grown in peptone broth, in the absence of the nematodes, the bacteria produce a protein toxin complex that is lethal when fed to, or injected into the hemolymph of, Manduca sexta larvae and several other insect species. The toxin purified as a protein complex which has an estimated molecular weight of 1,000,000 and contains no protease, phospholipase, or hemolytic activity and only a trace of lipase activity. The purified toxin possesses insecticidal activity whether injected or given orally. Analyses of the denatured complex by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed it to be composed of several protein subunits ranging in size from 30 to 200 kDa. The complex was further separated by native gel electrophoresis into three components, two of which retained insecticidal activity. The purified native toxin complex was found to be active in nanogram concentrations against insects representing four orders of the classInsecta.

Preparation and Characterization of Chitosan-Based Core–Shell Microcapsules Containing Clove Oil

2015 ◽  
Vol 15 (1) ◽  
pp. 600-605 ◽  
Author(s):  
Ping Jiang ◽  
Duxin Li ◽  
Ya Xiao ◽  
Xingxing Yang ◽  
Yuejun Liu
Keyword(s):  
Core Shell ◽  
Clove Oil

scholarly journals Characterization of Two Novel Bacillus thuringiensis Cry8 Toxins Reveal Differential Specificity of Protoxins or Activated Toxins against Chrysomeloidea Coleopteran Superfamily

Toxins ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 642
Author(s):  
Changlong Shu ◽  
Guixin Yan ◽  
Shizhi Huang ◽  
Yongxin Geng ◽  
Mario Soberón ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Genome Sequencing ◽  
Insecticidal Activity ◽  
Insect Pest ◽  
Anoplophora Glabripennis ◽  
Insecticidal Proteins ◽  
Holotrichia Parallela ◽  
Larval Stages ◽  
Differential Toxicity

Scarabaeoidea and Chrysomeloidea insects are agriculture-destructive coleopteran pests. Few effective Bacillus thuringiensis (Bt) insecticidal proteins against these species have been described. Bt isolate BtSU4 was found to be active against coleopteran insects. Genome sequencing revealed two new cry8 genes in BtSU4, designated as cry8Ha1 and cry8Ia1. Both genes expressed a 135 kDa protoxin forming irregular shape crystals. Bioassays performed with Cry8Ha1 protoxin showed that it was toxic to both larvae and adult stages of Holotrichia parallela, also to Holotrichia oblita adults and to Anoplophora glabripennis larvae, but was not toxic to larval stages of H. oblita or Colaphellus bowringi. The Cry8Ia1 protoxin only showed toxicity against H. parallela larvae. After activation with chymotrypsin, the Cry8Ha1 activated toxin lost its insecticidal activity against H. oblita adults and reduced its activity on H. parallela adults, but gained toxicity against C. bowringi larvae, a Chrysomeloidea insect pest that feeds on crucifer crops. The chymotrypsin activated Cry8Ia1 toxin did not show toxicity to any one of these insects. These data show that Cry8Ha1 and Cry8Ia1 protoxin and activated toxin proteins have differential toxicity to diverse coleopteran species, and that protoxin is a more robust protein for the control of coleopteran insects.

Sign in / Sign up

Export Citation Format

Share Document