scholarly journals Larvicidal Activity against Aedes aegypti and Chemical Characterization of the Inflorescences of Tagetes patula

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Letícia Maria Krzyzaniak ◽  
Tânia Mara Antonelli-Ushirobira ◽  
Gean Panizzon ◽  
Ana Luiza Sereia ◽  
José Roberto Pinto de Souza ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Insecticidal Activity ◽  
Chemical Characterization ◽  
Reversed Phase ◽  
Acetone Extract ◽  
Tagetes Patula ◽  
Larval Population

The crude acetone extract (CAE) of defatted inflorescences of Tagetes patula was partitioned into five semipurified fractions: n-hexane (HF), dichloromethane (DF), ethyl acetate (EAF), n-butanol (BF), and aqueous (AQF). BF was fractionated by reversed-phase polyamide column chromatography, obtaining 34 subfractions, which were subjected to HSCCC, where patuletin and patulitrin were isolated. CAE and the fractions BF, EAF, DF, and AQF were analyzed by LC-DAD-MS, and patuletin and patulitrin were determined as the major substances in EAF and BF, respectively. BF was also analyzed by HPLC and capillary electrophoresis (CE), and patulitrin was again determined to be the main substance in this fraction. CAE and the semipurified fractions (750, 500, 300, 100, and 50 mg/L) were assayed for larvicidal activity against Aedes aegypti, with mortality rate expressed as percentage. All fractions except AQF showed insecticidal activity after 24 h exposure of larvae to the highest concentration. However, EAF showed the highest activity with more than 50% reduction in larval population at 50 mg/L. The insecticidal activity observed with EAF might have been due to the higher concentration of patuletin present in this fraction.

scholarly journals Larvicidal Enzyme Inhibition and Repellent Activity of Red Mangrove Rhizophora mucronata (Lam.) Leaf Extracts and Their Biomolecules against Three Medically Challenging Arthropod Vectors

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3844
Author(s):  
Sengodan Karthi ◽  
Karthic Uthirarajan ◽  
Vinothkumar Manohar ◽  
Manigandan Venkatesan ◽  
Kamaraj Chinnaperumal ◽  
...  
Keyword(s):  
Larvicidal Activity ◽  
Mosquito Control ◽  
Chemical Characterization ◽  
Acetone Extract ◽  
Bioactive Molecules ◽  
Repellent Activity ◽  
Rhizophora Mucronata ◽  
Mosquito Vectors ◽  
Leaf Extracts ◽  
Red Mangrove

The larvicidal potential of crude leaf extracts of Rhizophora mucronata, the red mangrove, using diverse solvent extracts of the plant against the early fourth instar larvae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti mosquito vectors was analyzed. The acetone extract of R. mucronata showed the greatest efficacy: for Cx. quinquefasciatus (LC50 = 0.13 mg/mL; LC90 = 2.84 mg/mL), An. stephensi (LC50 = 0.34 mg/mL; LC90 = 6.03 mg/mL), and Ae. aegypti (LC50 = 0.11 mg/mL; LC90 = 1.35 mg/mL). The acetone extract was further fractionated into four fractions and tested for its larvicidal activity. Fraction 3 showed stronger larvicidal activity against all the three mosquito larvae. Chemical characterization of the acetone extract displayed the existence of several identifiable compounds like phytol, 3,7,11,15-tetramethyl-2-hexadecen-1-ol, 1-hexyl-2-nitrocyclohexane, eicosanoic acid etc. Enzyme assay displayed that R. mucronata active F3-fractions exert divergent effects on all three mosquitos’ biochemical defensive mechanisms. The plant fractions displayed significant repellent activity against all the three mosquito vectors up to the maximum repellent time of 210 min. Thus, the bioactive molecules in the acetone extract of R. murconata leaves showed significant larvicidal and enzyme inhibitory activity and displayed novel eco-friendly tool for mosquito control.

scholarly journals Insecticidal Activity of Bacillus laterosporus

1998 ◽  
Vol 64 (7) ◽  
pp. 2723-2725 ◽  
Author(s):  
Margarita V. Orlova ◽  
Tatyana A. Smirnova ◽  
Lyudmila A. Ganushkina ◽  
Victoria Y. Yacubovich ◽  
Roudolf R. Azizbekyan
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Insecticidal Activity ◽  
Culex Pipiens ◽  
Anopheles Stephensi ◽  
Crystalline Inclusions ◽  
Bacillus Laterosporus

ABSTRACT The Bacillus laterosporus strains 921 and 615 were shown to have toxicity for larvae of the mosquitoes Aedes aegypti, Anopheles stephensi, and Culex pipiens. The larvicidal activity of B. laterosporus was associated with spores and crystalline inclusions. Purified B. laterosporus 615 crystals were highly toxic for Aedes aegypti and Anopheles stephensi.

scholarly journals Priprioca (Cyperus articulatus var. nodosus, Cyperaceae) Hydrolate as Larvicidal against Aedes aegypti

2020 ◽  
pp. 91-98
Author(s):  
Inês Ribeiro Machado ◽  
Keila Rêgo Mendes ◽  
Adriano Rodrigues De Paula ◽  
Michelly Rios Arévalo ◽  
Amanda Sousa Silva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Plant Extracts ◽  
Chemical Characterization ◽  
Bioactive Substances ◽  
Commercial Products ◽  
Larvicidal Effect ◽  
The Third ◽  
Hydrolysis Of

Dengue cases have grown significantly in Brazil in recent years. Studies with plant extracts show the insecticidal potential of bioactive substances that become candidates for future commercial products. This work evaluated the larvicidal effect of essential oil, without hydrolysis of Cyperus articulatus var. nodosus against the third and fourth wide stage of Aedes aegypti, which reduces larvae survival by as much as the 3th and 4th instar of Ae. aegypti. A chemical characterization of the hydrogen skeletons was performed by the GC-MS, revealing verbenone (%), trans-sabinol (%) and mirtenol (%) as major compounds. 


Characterization and expression of a novel holotype insecticidal crystal protein gene from nativeBacillus thuringiensisBM59-2

2010 ◽  
Vol 56 (2) ◽  
pp. 156-161 ◽  
Author(s):  
Aiping Zheng ◽  
Jun Zhu ◽  
Lingxia Wang ◽  
Shuangcheng Li ◽  
Qiming Deng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Insecticidal Activity ◽  
Protein Gene ◽  
Crystal Protein ◽  
Insecticidal Crystal Protein ◽  
Escherichia Coli Bl21

We characterized a novel holotype cry gene (cry52Ba1) harbored in a Bacillus thuringiensis isolate BM59-2 native to China that expresses a spherical crystal protein of about 80 kDa. In this study, the full length of the cry52Ba1 gene was cloned from this strain. Sequence analysis of the gene was also performed. Furthermore, cry52Ba1 was expressed in Escherichia coli BL21(DE)pLysS, and the resulting insecticidal activity showed that the Cry52Ba1 protein exhibited high larvicidal activity against Aedes aegypti (Diptera), with a lethal concentration 50 of 1.526 µg/mL (95% confidence: 0.740–3.499 µg/mL).

scholarly journals CHEMICAL COMPOSITION, LARVICIDAL ACTIVITY AND RESIDUAL EFFECT OF Pterodon polygalaeflorus (BENTH.) BENTH. (FABACEAE) FRUIT OIL EXTRACTS AGAINST Aedes aegypti (DIPTERA: CULICIDAE)

2020 ◽  
Vol 48 (4) ◽  
Author(s):  
Armanda Amando Teles de Menezes ◽  
Tayrrana Silva Beltrão ◽  
Liliane Sousa Silva ◽  
Heloisa Helena Garcia da Silva ◽  
Ionizete Garcia da Silva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Chemical Characterization ◽  
Residual Effect ◽  
Small Scale ◽  
Mosquito Vector ◽  
Germacrene D ◽  
Oil Extract ◽  
Fruit Oil

The purpose of this study was to investigate the larvicidal activity of Pterodon polygalaeflorus oil extract against the mosquito vector Aedes aegypti. For this, crushed P. polygalaeflorus fruit underwent solvent extraction to obtain the oil extract. The chemical characterization was performed by gas chromatography coupled to mass spectrometry. For the bioassays third instar larvae of Ae. aegypti were utilized. Tests were carried out to determine the larvicidal activity and the residual effect under laboratory conditions, as well as field screening (small scale).The major components of P. polygalaeflorus oil extract were, respectively, E-caryophyllene, germacrene D and bicyclogermacrene. Lethal concentrations of 50% and 90% were 36.5 and 64.8 μg/mL respectively. The solution presented a residual effect for seven days and the efficiency of the product was preserved under field conditions. The results encourage continuing studies with the oil extract of P. polygalaeflorus as a research target for bioinsecticides.KEY WORDS: Bioinsecticide; sucupira; terpenes; vector control.

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