Fluorescence microscope (Zeiss) v1

Author(s):  
x.chu not provided

How to operate the fluorescence microscope in the lab. Beware of safety hazards: This instrument uses led radiation. Do not stare at the operating lamp for a prolonged period of time in fluorescence mode.

Author(s):  
Brian Cross

A relatively new entry, in the field of microscopy, is the Scanning X-Ray Fluorescence Microscope (SXRFM). Using this type of instrument (e.g. Kevex Omicron X-ray Microprobe), one can obtain multiple elemental x-ray images, from the analysis of materials which show heterogeneity. The SXRFM obtains images by collimating an x-ray beam (e.g. 100 μm diameter), and then scanning the sample with a high-speed x-y stage. To speed up the image acquisition, data is acquired "on-the-fly" by slew-scanning the stage along the x-axis, like a TV or SEM scan. To reduce the overhead from "fly-back," the images can be acquired by bi-directional scanning of the x-axis. This results in very little overhead with the re-positioning of the sample stage. The image acquisition rate is dominated by the x-ray acquisition rate. Therefore, the total x-ray image acquisition rate, using the SXRFM, is very comparable to an SEM. Although the x-ray spatial resolution of the SXRFM is worse than an SEM (say 100 vs. 2 μm), there are several other advantages.


Author(s):  
Dawn N. Castillo ◽  
Timothy J. Pizatella ◽  
Nancy A. Stout

This chapter describes occupational injuries and their prevention. It describes in detail the causes of injuries and epidemiology of injuries. Occupational injuries are caused by acute exposure in the workplace to safety hazards, such as mechanical energy, electricity, chemicals, and ionizing radiation, or from the sudden lack of essential agents, such as oxygen or heat. This chapter describes the nature and the magnitude of occupational injuries in the United States. It provides data on risk of injuries in different occupations and industries. Finally, it discusses prevention of injuries, using a hierarchical approach to occupational injury control.


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