scholarly journals Root-to-shoot organogenesis in Citrus jambhiri Lush. v1 (protocols.io.bq4emyte)

protocols.io ◽  
2020 ◽  
Author(s):  
Tongbram Roshni ◽  
Madhumita Dasgupta ◽  
MANAS RANJAN ◽  
Paresh Chandra ◽  
Narendra Prakash
1992 ◽  
Vol 84 (4) ◽  
pp. 521-530
Author(s):  
Jacques Julliard ◽  
Lucienne Sossountzov ◽  
Yvette Habricot ◽  
Georges Pelletier

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 461f-462
Author(s):  
Guochen Yang ◽  
Marihelen Kamp-Glass

Alfalfa seeds of Cimarron VR, CW1446, CW2440, C94-118, C94-785, and WL311 were used as explants. BA, zeatin, and TDZ were evaluated on callus initiation, development, and shoot production. Callus initiation and development toward shoot organogenesis were enhanced when BA was added in the culture medium. Calli produced from BA treatments were compact, solid, and dark green. Similar results were obtained when zeatin was added in the culture medium. However, no shoots were produced from such calli. Multiple shoots were produced directly from each individual explant when TDZ was added to culture medium. However, when higher concentration of TDZ was used, number of shoots per explant was decreased, and dwarf shoots were produced. No apparent difference on shoot production was observed among the cultivars tested so far. Data on number of shoots per explant from two of these cultivars need to be statistically analyzed.


2012 ◽  
Vol 169 (14) ◽  
pp. 1401-1407 ◽  
Author(s):  
Hodaka Shishido ◽  
Yoko Miyamoto ◽  
Rika Ozawa ◽  
Shiduku Taniguchi ◽  
Junji Takabayashi ◽  
...  

1986 ◽  
Vol 93 (2) ◽  
pp. 231-239 ◽  
Author(s):  
S. Nemec ◽  
J. Syversten ◽  
Y. Levy

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1261
Author(s):  
Robin Lardon ◽  
Danny Geelen

Plant regeneration is essential for survival upon wounding and is, hence, considered to be a strong natural selective trait. The capacity of plant tissues to regenerate in vitro, however, varies substantially between and within species and depends on the applied incubation conditions. Insight into the genetic factors underlying this variation may help to improve numerous biotechnological applications that exploit in vitro regeneration. Here, we review the state of the art on the molecular framework of de novo shoot organogenesis from root explants in Arabidopsis, which is a complex process controlled by multiple quantitative trait loci of various effect sizes. Two types of factors are distinguished that contribute to natural regenerative variation: master regulators that are conserved in all experimental systems (e.g., WUSCHEL and related homeobox genes) and conditional regulators whose relative role depends on the explant and the incubation settings. We further elaborate on epigenetic variation and protocol variables that likely contribute to differential explant responsivity within species and conclude that in vitro shoot organogenesis occurs at the intersection between (epi) genetics, endogenous hormone levels, and environmental influences.


2021 ◽  
Vol 289 ◽  
pp. 110400
Author(s):  
Eveline Y.Y. Kong ◽  
Julianne Biddle ◽  
Mike Foale ◽  
Bart Panis ◽  
Stephen W. Adkins

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